Zafer Saad Alshehri, Abdullah D. Alanazi, Hanadi B. A. Baghdadi
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引用次数: 0
Abstract
Purpose
This study aimed to green synthesized copper nanoparticles (CNP) using Lupinus arcticus extract and assess the protoscolicidal effects and the effectiveness of these nanoparticles in experimental animal settings to combat hydatid cysts.
Methods
The protoscolicidal properties of CNP were examined in vitro on hydatid cyst PS via the eosin exclusion assay. The in vivo effectiveness of CNP at doses of 20, 40, and 80 mg/kg/day for 28 days on hydatid cyst-infected mice was determined by assessing the number, size, weight of hydatid cysts the gene expression levels of caspase-3, antioxidant (glutathione peroxidase (GPx) and superoxide dismutase (SOD)) and inflammatory cytokines (Interleukin-4 (IL-4), IL-10, and inducible nitric oxide synthase (iNOS)) in the infected mice were also assessed by Real-time PCE.
Results
Analyses revealed that the CNP had a spherical shape, ranging in size from 10 to 85 nm. The half-maximal inhibitory concentration (IC50) values for CNP against protoscoleces ranged from 39.9 to 341.3 µg/mL. Caspase-3 gene expression in the PS treated with CNP at 1/3 IC50, ½ IC50, and IC50 was 1.79-, 3.11-, and 5.39-fold change (p < 0.001). Upon administration of CNP, particularly at doses of 80 mg/kg, a significant decrease (p < 0.001) in hydatid cysts’ quantity, size, and weight was evident. CNP treatment caused a notable reduction in oxidative stress markers and inflammatory cytokines and increased the gene expression of antioxidant enzymes (p < 0.001), whereas it modulated the serum levels of liver function parameters.
Conclusion
The study indicated the potential of green-synthesized CNP for eliminating the protoscoleces and in managing hydatid cysts through leveraging its antioxidant and anti-inflammatory characteristics. Nevertheless, additional investigations are necessary to elucidate the precise mechanisms of action and evaluate its effectiveness in clinical trials.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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