Analysis of the Behavior of Trypanosoma cruzi in Culture Against Different Dosage Forms of Benznidazole: Experimental Insights

IF 1.5 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2025-09-03 DOI:10.1007/s11686-025-01125-9

Jaime López-Domínguez, Aracely López-Monteon, Paulina Ochoa-Martínez, Josue Rafael González-García, Rodolfo Peña-Rodríguez, Etienne Waleckx, Joel Moo-Millán, Angel Ramos-Ligonio

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Abstract

Introduction

This study aimed to investigate the influence of benznidazole (BZN) origin on its in vitro potency against various Trypanosoma cruzi strains.

Methods

Pure BZN, purified BZN, and the pharmaceutical formulation Abarax were evaluated for their activity against several parasite strains.

Results

Results demonstrated significant variability in BZN’s effectiveness, contingent upon both its specific form and the T. cruzi strain under investigation. Half-maximal inhibitory concentrations (IC₅₀ values) exhibited a wide range, indicating that the potency of BZN is not uniform across all its forms or against all parasite strains.

Conclusion

In conclusion, this study elucidates the complex interaction between BZN and T. cruzi. The parasite’s response to BZN can vary even within a single strain, influenced by the drug’s specific presentation. These findings underscore the critical importance of considering both parasite diversity and drug formulation when assessing the efficacy of treatments in laboratory assays.

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克氏锥虫对不同剂型苯并硝唑培养行为的实验分析

本研究旨在探讨苯并硝唑（BZN）产地对其体外抗克氏锥虫效力的影响。方法对纯BZN、纯化BZN和制剂Abarax对几种寄生虫的活性进行了评价。结果表明，BZN的有效性存在显著差异，这取决于其具体形式和所调查的克氏T.菌株。半数最大抑制浓度（IC50值）的变化范围很广，表明BZN在所有形式或对所有寄生虫品系的效力不是统一的。结论本研究阐明了BZN与克氏锥虫的复杂相互作用。寄生虫对BZN的反应甚至在单一菌株内也会变化，受药物特定表现的影响。这些发现强调了在实验室分析中评估治疗效果时同时考虑寄生虫多样性和药物配方的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.