Acta Parasitologica最新文献_第2页

Prevalence of Theileria Equi and Babesia Caballi and Associated Hemato-Biochemical Changes in Racing Horses in Erbil Province, Iraq.

IF 1.2 3区农林科学

Acta Parasitologica Pub Date : 2025-01-24 DOI: 10.1007/s11686-024-00987-9

Khalid Jabar Aziz, Lokman Taib Omer Al- Barwary, Nawzat Aboziad Issa, Muhsin Jamil Abdulwahid

{"title":"Prevalence of Theileria Equi and Babesia Caballi and Associated Hemato-Biochemical Changes in Racing Horses in Erbil Province, Iraq.","authors":"Khalid Jabar Aziz, Lokman Taib Omer Al- Barwary, Nawzat Aboziad Issa, Muhsin Jamil Abdulwahid","doi":"10.1007/s11686-024-00987-9","DOIUrl":"https://doi.org/10.1007/s11686-024-00987-9","url":null,"abstract":"Purposes: This study aimed to determine the prevalence of Theileria equi (T. equi) and Babesia caballi (B. caballi) in racing horses in Erbil Province, Iraq, and to assess the associated hemato-biochemical changes in infected animals.Methods: A total of 196 horses, including 155 clinically healthy and 41 clinically suspected infected animals, were examined using Giemsa-stained blood films and PCR to determine the prevalence rates. Additionally, the hemato-biochemical parameters of infected horses were compared to a non-infected control group (n = 10).Results: The prevalence of T. equi and B. caballi was determined using two diagnostic methods. Giemsa-stained blood smear analysis revealed prevalence rates of 15.8% (31/196) for T. equi and 5.1% (10/196) for B. caballi. In comparison, conventional PCR targeting the 18 S rRNA gene detected higher prevalence rates of 22.44% (44/196) for T. equi and 8.2% (16/196) for B. caballi. The prevalence of T. equi was significantly higher than that of B. caballi (P < 0.001). Infected horses exhibited significant hematological alterations, including microcytic anemia, with notable reductions in RBCs, Hb, and PCV, along with increased WBCs, lymphocytes and basophils compared to the healthy control group. Biochemical analysis revealed significant increases in AST, ALT, ALP, and total serum bilirubin, and decreases in total serum protein, albumin, calcium, and glucose in infected horses.Conclusion: These findings highlight the urgent need for heightened awareness, effective diagnostic methods, and targeted treatment strategies to combat equine piroplasmosis among racing horses in Erbil Province, Iraq.","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"38"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Possible Coexistence of Microsporidia, Plasmodium falciparum and Wuchereria bancrofti in Anopheles gambiae s.l within Ahmadu Bello University, Zaria, Nigeria.

IF 1.2 3区农林科学

Acta Parasitologica Pub Date : 2025-01-24 DOI: 10.1007/s11686-024-00971-3

Mukhtar Aliyu, Abdulmalik Abdullahi Salman, Mohammed Auwal Ibrahim, Emmanuel Oluwadare Balogun, Mohammed Nasir Shuaibu

{"title":"Analysis of Possible Coexistence of Microsporidia, Plasmodium falciparum and Wuchereria bancrofti in Anopheles gambiae s.l within Ahmadu Bello University, Zaria, Nigeria.","authors":"Mukhtar Aliyu, Abdulmalik Abdullahi Salman, Mohammed Auwal Ibrahim, Emmanuel Oluwadare Balogun, Mohammed Nasir Shuaibu","doi":"10.1007/s11686-024-00971-3","DOIUrl":"https://doi.org/10.1007/s11686-024-00971-3","url":null,"abstract":"Purpose: Anopheles gambiae is a vector of Plasmodium falciparum and Wuchereria bancrofti. Endosymbionts are reported to block development of various parasites in mosquitoes. Microsporidia was reported to affect the development of P. falciparum in mosquitoes. Data on such observation is limited in Nigeria.Methods: Therefore, the prevalence of Microsporidia and its coinfection with W. bancrofti and P. falciparum in An. gambiae s.l was studied within Ahmadu Bello University, Zaria.Results: Of the 912 mosquitoes sampled, 124 were An. gambiae s.l The midgut assessment of the Anopheles mosquitoes using light microscopy and polymerase chain reaction (PCR) showed a 12% prevalence of a mono microsporidia infection with no coinfection with either P. falciparum or W. bancrofti. Only 4.03% of the An. gambiae s.l. were found to be coinfected with P. falciparum and W. bancrofti while no mosquito harboured all the microorganisms CONCLUSION: This data further supports the potential of Microsporidia as an antagonist for the development of pathogens in mosquitoes.","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"23"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Toxoplasma gondii in Brain Tissues of Pet Birds with Neurological Symptoms Using Loop-Mediated Isothermal Amplification (LAMP) and PCR.

IF 1.2 3区农林科学

Acta Parasitologica Pub Date : 2025-01-24 DOI: 10.1007/s11686-024-00983-z

Mohammad Neshatpour Esfahani, Majid Gholami-Ahangaran, William J Sullivan

{"title":"Detection of Toxoplasma gondii in Brain Tissues of Pet Birds with Neurological Symptoms Using Loop-Mediated Isothermal Amplification (LAMP) and PCR.","authors":"Mohammad Neshatpour Esfahani, Majid Gholami-Ahangaran, William J Sullivan","doi":"10.1007/s11686-024-00983-z","DOIUrl":"https://doi.org/10.1007/s11686-024-00983-z","url":null,"abstract":"Background: This study seeks to close this divide by assessing the occurrence of Toxoplasma gondii (T. gondii) in the brain tissues of pet birds displaying neurological symptoms, utilizing Nested Polymerase Chain Reaction (PCR) and Loop-mediated Isothermal Amplification (LAMP) methods. Furthermore, it aims to evaluate and contrast the sensitivity and specificity of different diagnostic procedures. Seventy bird samples, consisting of 50 sick and 20 asymptomatic samples, were assessed using various methods. Brain tissue DNA was isolated and then analyzed using nested PCR and LAMP assays targeting the T. gondii B1 gene and RE gene. Sensitivity and specificity assessments were conducted, and statistical evaluations were made using SPSS software.Results: Our findings revealed that no T. gondii cysts were detected in the smear preparations. However, T. gondii DNA was found in 6% of the sick birds using nested PCR, and LAMP identified T. gondii DNA in 8% of these individuals. Both techniques demonstrated a high level of specificity, with LAMP showing higher sensitivity and faster processing times than nested PCR. These results significantly contribute to our understanding of toxoplasmosis and highlight the reliability and potential for the widespread use of these diagnostic techniques, providing reassurance and confidence in their effectiveness.Conclusion: The study underscores the importance of molecular diagnostics in promptly identifying and treating toxoplasmosis in birds. This enhances our understanding and control of the disease and underscores the significant impact of this research. The potential for further studies using more extensive and diverse bird populations to enhance surveillance and prevention strategies against T. gondii infections is promising. It should be actively pursued, giving us hope for a better future in avian health.","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"40"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Arum rupicola Boiss rupicola Extracts on Visceral Larva Migrans in Mice.

IF 1.2 3区农林科学

Acta Parasitologica Pub Date : 2025-01-24 DOI: 10.1007/s11686-024-00970-4

Gozde Nur Akkus, Tugrul Atalay, Sinem Akdeniz, Husamettin Ekici, Dincer Yildiz, I Safa Gurcan, Kader Yildiz

{"title":"Effect of Arum rupicola Boiss rupicola Extracts on Visceral Larva Migrans in Mice.","authors":"Gozde Nur Akkus, Tugrul Atalay, Sinem Akdeniz, Husamettin Ekici, Dincer Yildiz, I Safa Gurcan, Kader Yildiz","doi":"10.1007/s11686-024-00970-4","DOIUrl":"https://doi.org/10.1007/s11686-024-00970-4","url":null,"abstract":"Purpose: In the present study, the effects of leaf and rhizome extracts of Arum rupicola Boiss rupicola were searched on the infective stage Toxocara canis larvae (L3) in the experimentally infected mice.Methods: Four-six week-old male BALB/c mice were divided into eight groups (G1-8, each group consisted of 7 mice), and they were infected orally with 500 T. canis eggs with L3. After 24 h, the following treatment protocol was applied to the groups for five days: G-1: Albendazole (ABZ) (100 mg/kg), G-2: Leaf Extract (10 mg/mL) + ABZ (100 mg/kg), G-3: Leaf Extract (40 mg/mL) + ABZ (100 mg/kg), G-4: Rhizome Extract + ABZ (100 mg/kg), G-5: Leaf Extract (10 mg/mL), G-6: Leaf Extract (40 mg/mL), G-7: Rhizome extract, G-8: Negative control. The necropsy was performed on the 45th day of experimental infection.Results: The rhizome extract significantly reduced the effectiveness of ABZ compared to leaf extracts. The number of larvae in rhizome extract-treated mice (G7) was similar to ABZ-treated mice (G1) (P < 0.05). The larval number in mice administered leaf extract (10 mg/mL and 40 mg/mL) was slightly decreased. But no significant difference was detected in the larval number depending on the dose of leaf extracts (P > 0.05).Conclusion: The administration of the leaf and rhizome extracts did not contribute to the effectiveness of ABZ. Also the effect of ABZ on the larvae number obtained from the tissues was reduced by the rhizome extract. The larvae number in the group in which rhizome extract was given alone was almost close to the group in which ABZ was administered. The results provide insight for further research on the antiparasitic properties of A. rupicola Boiss rupicola rhizome extract.","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"31"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Experimental Congenital Toxoplasmosis on the Thyroid Gland: Histopathological and Immunobiochemical Indices Assessment.

IF 1.2 3区农林科学

Acta Parasitologica Pub Date : 2025-01-24 DOI: 10.1007/s11686-024-00969-x

Hanan Abd Elgawad, Manar S Elmehankar, Nairmen Nabih, Heba Sheta, Soha I Awad

{"title":"Impact of Experimental Congenital Toxoplasmosis on the Thyroid Gland: Histopathological and Immunobiochemical Indices Assessment.","authors":"Hanan Abd Elgawad, Manar S Elmehankar, Nairmen Nabih, Heba Sheta, Soha I Awad","doi":"10.1007/s11686-024-00969-x","DOIUrl":"https://doi.org/10.1007/s11686-024-00969-x","url":null,"abstract":"Purpose: The thyroid gland is one of the most vital endocrine organs. It is responsible for the synthesis and secretion of hormones principally triiodothyronine (T3) and thyroxine (T4). These hormones play a significant role in the functions and the metabolism of the body. The thyroid gland could be affected by several infections, among them Toxoplasma gondii. Congenital toxoplasmosis took place when the parasite reached the developing fetus and infected any nucleated cells within it. This study assessed the effect of experimental congenital toxoplasmosis on the thyroid gland structure and function.Methods: We used 34 Wistar rats and allocated them into two groups: normal control group (17 rats) and congenital toxoplasmosis group (17 rats). After euthanasia, the brain and the thyroid gland was assessed through histopathological examination. Thyroid functions examination was performed through measuring the serum levels of T3, thyroxine T4, and thyroid stimulating hormone (TSH). Moreover, serum levels of thyroid antibodies [thyroid-peroxidase antibody (TPO-Ab) and anti-thyroglobulin (TG-Ab)] were examined.Results: The examination of thyroid tissues of the congenital toxoplasmosis group showed decreased or absent colloid secretion. About 47.1% of follicles showed degeneration with different grades. Parafollicular cells hyperplasia were observed in 23.6% of specimens. The serum concentrations of T3, T4, and TSH were significantly higher in congenital toxoplasmosis group than the control group. The congenital toxoplasmosis group had lower serum concentrations of TPO-Abs than the control group.Conclusion: These results indicated that congenital Toxoplasma infection could result in a central hyperthyroidism state with alteration of thyroid gland structure in offspring.","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"43"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trichomoniasis in Men: A Neglected Factor in Male Infertility?

IF 1.2 3区农林科学

Acta Parasitologica Pub Date : 2025-01-24 DOI: 10.1007/s11686-024-00984-y

Seyed Ali Hosseini, Mohammad Matini, Maryam Bahmanzadeh, Reza Aslani, Faeze Foroughi-Parvar

{"title":"Trichomoniasis in Men: A Neglected Factor in Male Infertility?","authors":"Seyed Ali Hosseini, Mohammad Matini, Maryam Bahmanzadeh, Reza Aslani, Faeze Foroughi-Parvar","doi":"10.1007/s11686-024-00984-y","DOIUrl":"https://doi.org/10.1007/s11686-024-00984-y","url":null,"abstract":"Purpose: This study is aimed to detect the frequency of trichomoniasis, a sexually transmitted infection caused by an anaerobic protozoan Trichomonas vaginalis, in men referred to the Fertility and Infertility Research Center Hamadan University of Medical Sciences.Methods: In this cross-sectional study, a group of 197 male volunteers who sought medical attention for issues related to infertility participated. The urine and semen samples were collected in sterile conditions. Both urine and semen sediment were promptly examined under a microscopy to detect the presence of motile trophozoites. Subsequently, 50 µl of urine sediment were inoculated into the Dorset culture medium, whereas 50 µl of semen sediment were inoculated into the Diamond culture (TYI-S-33) and incubated at 35.5oC. Finally, the processed urine samples were used for molecular analysis.Results: The investigated subjects had an average age ranging from 36 to 40 years. There were 181 volunteers with fertility issues, outnumbering the 16 individuals with normal fertility. Spermogram analysis showed that 48% of participants had non-motile or progressive sperms, and 48% had abnormalities in sperm morphology. T. vaginalis was not detected through microscopic assessment, but PCR and sequence analysis revealed one case in a 33-year-old infertile individual, who had only 0.3% normal sperm with 19% motility. The isolated T.vaginalis was found to have the G genotype based on the sequencing analysis.Conclusion: Trichomoniasis in males was considered unimportant, with the belief that it would improve on its own. This study suggests that parasites can be considered as one of the male infertility factors, however, the impact is not fully understood. This investigation confirmed the effectiveness of molecular techniques in detecting trichomoniasis in males.","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"44"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Potential Role of Toxoplasma gondii Infection in Multiple Sclerosis Development: A Seroepidemiological Study in North Khorasan Province, Iran.

IF 1.2 3区农林科学

Acta Parasitologica Pub Date : 2025-01-24 DOI: 10.1007/s11686-024-00962-4

Amirali Ghahremani, Hasan Namdar Ahmadabad, Seyed Mohammad Javadzadeh, Reza Shafiei

{"title":"The Potential Role of Toxoplasma gondii Infection in Multiple Sclerosis Development: A Seroepidemiological Study in North Khorasan Province, Iran.","authors":"Amirali Ghahremani, Hasan Namdar Ahmadabad, Seyed Mohammad Javadzadeh, Reza Shafiei","doi":"10.1007/s11686-024-00962-4","DOIUrl":"https://doi.org/10.1007/s11686-024-00962-4","url":null,"abstract":"Pourpose: This study aimed to investigate the seroepidemiological status of Toxoplasma gondii (T. gondii) infection in Multiple Sclerosis (MS) patients compared to controls.Methods: The present study included 98 MS patients and 100 controls. Demographic and clinical information collected through interviews, electronic medical records, and clinical examinations. Serum samples analyzed for anti-T. gondii IgG and IgM antibodies using ELISA.Results: A significant difference observed in the seroprevalence of anti-T. gondii IgG between 49 MS patients (50%) and 33 controls (33%). MS patients with seropositive anti-T. gondii IgG had significantly different age, Expanded Disability Status Scale (EDSS) scores, MS phenotypes, age of diagnosis, and age of onset compared to seronegative patients. Serum levels of anti-T. gondii IgG significantly elevated in the case group compared to the control group (277.8 ± 28.61 vs. 137.1 ± 9.52, P < 0.0001). In controls, all subjects were seronegative for anti-T. gondii IgM; three MS cases were positive, showing no significant difference in anti-T. gondii IgM seroprevalence between groups.Conclusion: The study suggests a potential association between T. gondii infection and MS, indicating that anti-T. gondii IgG positivity may correlate with MS phenotypes and influence disease progression. This suggests that chronic toxoplasmosis may be risk factor for MS.","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"32"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxoplasma gondii Infection in the Male Reproductive System: A Systematic Review.

IF 1.2 3区农林科学

Acta Parasitologica Pub Date : 2025-01-24 DOI: 10.1007/s11686-024-00978-w

Pablo Tabares Tejada, Walter D Cardona Maya

{"title":"Toxoplasma gondii Infection in the Male Reproductive System: A Systematic Review.","authors":"Pablo Tabares Tejada, Walter D Cardona Maya","doi":"10.1007/s11686-024-00978-w","DOIUrl":"https://doi.org/10.1007/s11686-024-00978-w","url":null,"abstract":"Purpose: Toxoplasmosis is a worldwide widespread parasitic infection; it affects about 30% of the global population, either through acute toxoplasmosis or its sequels. Even though the male reproductive system is not the primary target for Toxoplasma gondii (T. gondii), studies have inquired into the possibility of presenting repercussions in this system directly or indirectly due to toxoplasmosis. Therefore, this systematic literature review aims to summarize the available evidence on the effects of infection caused by T. gondii on the male reproductive tract.Methods: We searched PubMed, Scopus, LILACS and Google Scholar until June 2024 to identify studies of T. gondii and the human male reproductive system. Finally, we analyzed 24 papers published between 1986 and 2024. The Joanna Briggs Institute (JBI) Critical Appraisal Checklist was used to assess the potential risk of bias and the quality of the results.Results: Infertility is a multicausal issue, including various stages in which the infection caused by T. gondii could interfere, but the mechanisms are not fully understood yet. Studies in animals, particularly rats, have shown the harmful effects of the parasite on sperm performance and endocrine function. In the same way, sexual transmission of T. gondii has been extensively studied in animals, with the parasite detected in the semen of various species. In humans, this transmission route remains theoretical due to study limitations. However, discrepancies in findings call for further research to understand the mechanisms and make the T. gondii's infection impact on the male reproductive system a topic of growing interest.Conclusion: Acute and chronic infection by T. gondii in the male reproductive system is a topic of growing interest due to its possible implications for reproductive health.","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"29"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Approaches of Inducing Tolerance to Murine Schistosomiasis mansoni applying Biomphalaria and Bulinus Proteins.

IF 1.2 3区农林科学

Acta Parasitologica Pub Date : 2025-01-24 DOI: 10.1007/s11686-025-00988-2

Hanan S Mossalem, Sami Mohamed Nasr, Azza Moustafa Fahmy, Shimaa Atta, Gehan Labib Abuelenain

{"title":"Approaches of Inducing Tolerance to Murine Schistosomiasis mansoni applying Biomphalaria and Bulinus Proteins.","authors":"Hanan S Mossalem, Sami Mohamed Nasr, Azza Moustafa Fahmy, Shimaa Atta, Gehan Labib Abuelenain","doi":"10.1007/s11686-025-00988-2","DOIUrl":"https://doi.org/10.1007/s11686-025-00988-2","url":null,"abstract":"Background: The freshwater snails Biomphalaria alexandrina and Bulinus trancatus are key contributors to the transmission of S. mansoni and S.haematobium, respectively, for being their intermediate hosts.Objectives: This research study aimed to investigate the potency of the nucleoproteins (NPs) extracted from both snail species on the host immune reactions as an approach to developing a potential vaccine.Methods: Three groups of six-week-old Swiss-Webster mice (n = 18; 15-20 g each) were injected intraperitoneally for three consecutive weeks with single doses (once a week) of B. alexandrina, B. truncatus, or a mixture of their nucleoproteins (50 µg each). On day 21st, the nucleoprotein-treated mice altogether, with six more mice, received subcutaneously S. mansoni cercariae (60/mouse). Eight weeks later, the experimental mice were sacrificed for evaluation of certain parasitological, molecular and immunological responses.Results: The data of mice immunized with the various types of nucleoproteins showed a significant increase of FAS/R gene expressions in hepatic tissues and anti-IgG antibody levels in sera on the one hand and a significant decrease of worm loads and β-actin/R gene expression levels on the other hand when compared to the infected control mice.Conclusion: These findings highlight the role of snails in immunomodulation and shed light on the possibility of antagonizing effects that might occur when the nucleoproteins of different species are mixed. Moreover, this research study might promote the literature spotting the importance of snail proteins against schistosomiasis.","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"41"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Record and Identification of a Microsporidian Pathogen, Nosema Maddoxi in the Population of Brown Marmorated Stink Bug Halyomorpha Halys (Hemiptera: Pentatomidae) in Türkiye.

IF 1.2 3区农林科学

Acta Parasitologica Pub Date : 2025-01-24 DOI: 10.1007/s11686-024-00963-3

Samet Eker, Mustafa Yaman, Ömer Ertürk, Tuğba Sağlam Güvendik, Gönül Algı

{"title":"First Record and Identification of a Microsporidian Pathogen, Nosema Maddoxi in the Population of Brown Marmorated Stink Bug Halyomorpha Halys (Hemiptera: Pentatomidae) in Türkiye.","authors":"Samet Eker, Mustafa Yaman, Ömer Ertürk, Tuğba Sağlam Güvendik, Gönül Algı","doi":"10.1007/s11686-024-00963-3","DOIUrl":"https://doi.org/10.1007/s11686-024-00963-3","url":null,"abstract":"Purpose: The brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae), is an invasive and a highly polyphagous species with a strong dispersal capacity. Unfortunately, there is currently no effective control method that can prevent or reduce the economic loss caused by this pest. Among natural enemies, microsporidia cause infections in insects so that they can generally shorten life span, reduce fertility and inhibit growth.Methods: Current study involved field study, light and electron microscopy and molecular phylogenic analyses of a microsporidian pathogen in the populations of H. halys in Türkiye.Results: The microsporidian infection were mostly observed in malpighian tubules and fatty tissue filled with high spore density. Fresh uninucleate spores are oval, measued as 3.73 × 2.01 µm in dimensions. The mature spore wall is relatively thick and measures 75-85 nm and consists of a clear endospore (40-50 nm) and an electron-dense, uniform, thin exospore (25-30 nm). The polar filament is isofilar, 85-110 mm in diameter and has 7-8 coils. It is found to be most closely related to Nosema maddoxi isolate Mn.6 isolated from H. halys in Georgia in phylogenetic tree.Conclusion: The spore morphology and structural features of the isolate from H. halys identify it as Nosema maddoxi. The phylogenetic analyses confirm both light and electron microscopic observations. This is the first microsporidian record from H. halys and also from the order Hemiptera in Türkiye. It is also confirmed that the invasive pest, H. halys carries its natural pathogen, N. maddoxi to new geographical locations during its distribution.","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":"21"},"PeriodicalIF":1.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0