Acta Parasitologica最新文献

{"title":"Effects of Hydatid Cyst Fluid on Inflammation and Epithelial-Mesenchymal Transition in Colorectal Adenocarcinoma (Caco-2) Cell Line.","authors":"Emin Yagmur, İpek Baysal, Serra Örsten, Fatma İnanç Tolun","doi":"10.1007/s11686-025-01086-z","DOIUrl":"10.1007/s11686-025-01086-z","url":null,"abstract":"<p><strong>Purpose: </strong>Anti-tumor immune responses and certain pathogens can exhibit various effects, including signals that reduce the risk of tumor formation or lead to cancer regression. Multiple studies have reported that infectious agents and the products of a wide range of host structures can modulate cancer development and growth positively or negatively and regulate the activation of immune responses. Numerous studies have reported that the parasite Echinococcus granulosus may have anti-cancer or carcinogenic effects on cancer cell proliferation in various cell cultures and animal models. The primary purpose of the study is to investigate the effect of animal-derived hydatid cyst fluid (HCF) at various concentrations (1/2, 1/3, 1/5) on the viability of human colorectal adenocarcinoma (Caco-2) and human colon epithelial (CoEpi) cell lines using the cell proliferation assay (XTT).</p><p><strong>Methods: </strong>Subsequently, the study aims to investigate cytokine concentrations and gene expression profiles of inflammatory cytokines TNF-α and IL-4, as well as epithelial-mesenchymal transition (EMT) regulatory signaling proteins TGF- β1, vimentin and E-cadherin, using ELISA and RT-PCR methods, respectively.</p><p><strong>Results: </strong>Following the HCF application, EMT was consistently detected in the Caco-2 cell line compared to the CoEpi cell line in the 1/5 volume application group, as confirmed by ELISA, RT-PCR and cell proliferation assays. On the other hand, a linear relationship was observed between the levels of TGF-β1 and TNF-α, which regulate the pro-inflammatory signaling mechanism based on the cell micro-environment and the decrease in cell viability. As the HCF concentration volume decreased (1/5), an increase in cell viability was observed (P < 0.01), along with an increase in TGF-β1 and TNF-α levels. Otherwise, in Caco-2 cells, as the HCF application concentrations increased (1/2), significant decreases in TGF-β1 and TNF-α levels, as well as in cell viability, were observed (P < 0.01).</p><p><strong>Conclusions: </strong>In this context, the common antigen-receptor structure between E. granulosus and cancer may modulate the signals of the immune response it regulates, affecting immune system cells and contributing to the progression of tumor cells.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 4","pages":"146"},"PeriodicalIF":1.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12234636/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cercarial Dermatitis in Norway - An Emerging Zoonotic Disease.","authors":"Arnulf Soleng, Thora Gundersen, Heidi Lindstedt","doi":"10.1007/s11686-025-01083-2","DOIUrl":"10.1007/s11686-025-01083-2","url":null,"abstract":"","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 4","pages":"143"},"PeriodicalIF":1.2,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence and Risk Variables Related To T. Gondii Infections in Sheep and Goats, Jimma Arjo District, East Wallaga Zone, Oromia, Ethiopia.","authors":"Misganu Bulla, Efrem Degneh, Tigist Ashagire","doi":"10.1007/s11686-025-01082-3","DOIUrl":"https://doi.org/10.1007/s11686-025-01082-3","url":null,"abstract":"<p><strong>Purpose: </strong>The research was carried out in the Jimma Arjo district between January and July 2022 to assess the seroprevalence and risk variables linked to infections caused by T. gondii in small ruminants.</p><p><strong>Methods: </strong>An indirect ELISA was used to assess blood samples from 267 randomly selected small ruminants (111 goats' and 156 sheep) for T. gondii IgG antibodies. The data was evaluated with SPSS version 25 and STATA 14.0.</p><p><strong>Result: </strong>An overall seroprevalence of T. gondii was 54.7% (CI = 48.5-60.7). Toxoplasma gondi seroprevalence was significantly higher in sheep (62.2%) compared to goats (44.1%) (P = 0.00). The findings of the research revealed that age and species were significantly (P < 0.05) linked with exposure to T. gondii. Adult sheep and goats had a higher T. gondii infection (67.3%) than in younger animals (34.3%) (P = 0.00). While the seroprevalence did not vary statistically between the villages under study (P = 0.57). Tibe Chafe (61.4%) had the highest seroprevalence, followed by Jarso Kiltu (56.1%), Jamo Gambala (55.7%), and Hidhe village (48.5%) (< 0xC8 > 2 = 2.02; P = 0.57).</p><p><strong>Conclusion: </strong>In conclusion, it was discovered that T. gondii was widespread in sheep and goats across the research areas, and the infection was found to be strongly associated with the animals' age, species and abortion history. Therefore, in order to fully understand the epidemiology of diseases, it is important to conduct molecular studies on all susceptible animals. Additionally, health education to prevent cat feces from contaminating animals' food is necessary.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 4","pages":"145"},"PeriodicalIF":1.2,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Evolution of the Order Siphonaptera Inferred from the First Mitogenome of the Family Pygiopsyllidae.","authors":"Xiaoxia Lin, Ju Pu, Wenge Dong","doi":"10.1007/s11686-025-01051-w","DOIUrl":"https://doi.org/10.1007/s11686-025-01051-w","url":null,"abstract":"","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 4","pages":"144"},"PeriodicalIF":1.2,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome diversity in Haemaphysalis flava (life stage-host dependent) and Haemaphysalis longicornis ticks with zoonotic implications in Nantong, China.","authors":"Jing Su, Wei-Bing Zhang, Yu-Jie Chen, Bin Sun, Yun-Peng Zhai, Jian-Ming Yuan","doi":"10.1007/s11686-025-01088-x","DOIUrl":"https://doi.org/10.1007/s11686-025-01088-x","url":null,"abstract":"<p><strong>Purpose: </strong>This study characterized the microbial communities of Haemaphysalis flava (H. flava) and Haemaphysalis longicornis (H. longicornis), in Nantong, China, and assessed the zoonotic implications.</p><p><strong>Methods: </strong>We collected both on-host and off-host ticks and performed 16S rRNA amplicon sequencing. Subsequent bioinformatic analyses included taxonomic composition assessment, community diversity evaluation, differential abundance analysis, interspecies abundance correlation and functional inference.</p><p><strong>Results: </strong>Rickettsia dominated in H. flava (77.31%), while H. longicornis exhibited higher abundances of Stenotrophomonas (10.78%), Coxiella (10.04%), and Psychrobacter (9.70%). Comparative analyses of life stages and host associations were only performed for H. flava due to limited sample sizes of H. longicornis across developmental stages. Rickettsia was enriched in on-host H. flava (90.41-90.51%) compared to off-host specimens (46.12%). α-diversity analysis showed higher microbial richness in off-host nymphs than in on-host adults. β-diversity revealed strong species-specific clustering. Network analysis demonstrated more complex microbial interactions in adult ticks. Pathogen screening detected Rickettsia japonica (R. japonica, host-specific to H. flava), Ehrlichia ewingii (E. ewingii), and Anaplasma bovis (A. bovis). Functional prediction highlighted elevated B vitamin biosynthesis pathways in nymphs, aligning with Coxiella-like endosymbionts (CLEs)'s putative nutritional role.</p><p><strong>Conclusion: </strong>This study emphasizes the importance of enhanced tick surveillance and regular pathogen screening in domestic animals, particularly for spotted fever group (SFG) Rickettsia. CLEs may exhibit stage-specific abundance patterns aligned with the host's developmental nutritional requirements. These findings highlight the need for integrated One Health surveillance to mitigate tick-borne disease threats.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 4","pages":"142"},"PeriodicalIF":1.2,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence of Ascaris Lumbricoides Over 20 Years in the Population of the United Mexican States (2003-2022) and Projection Through 2030.","authors":"José Manuel Hernández, Gabriela-Ibañez-Cervantes, Benjamín Nogueda-Torres, Minerva Camacho-Nuez, Alejandro D Camacho, Diego Fuentes-Domínguez, Gloria León-Avila","doi":"10.1007/s11686-025-01080-5","DOIUrl":"10.1007/s11686-025-01080-5","url":null,"abstract":"<p><strong>Purpose: </strong>Soil-transmitted helminth infections affect people who live in areas with poor sanitation and low socioeconomic levels. Ascaris lumbricoides is one of the most frequent parasites worldwide and in México. The General Directorate of Epidemiology reports data related to infections weekly, and this study was based on that. The aim of this study was to analyze the SINAVE data for twenty years (2003-2022) to assess the incidence of ascariasis in the United Mexican States.</p><p><strong>Methods: </strong>We conducted a retrospective observational analysis of ascariasis from January 2003 to December 2022, classifying data by year, age group, state, and gender. A predictive model was used to estimate the new cases until 2030.</p><p><strong>Results: </strong>From 2003 to 2022, the total number of new cases registered in the whole country decreased by 81.2%. The geographical distribution of the new cases was more pronounced in the Neotropical region of Yucatan, Tabasco, and Chiapas States. Women and infants from 1 to 9 years old exhibited the highest incidence. The prediction model showed that in 2030, there will be an incidence higher than 15,000 new cases in the United Mexican States.</p><p><strong>Conclusions: </strong>This work indicates that Ascaris infection tends to decrease an is in accordance to our model, and could be related to hand hygiene and the look-down approach during the COVID-19 pandemic.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 4","pages":"139"},"PeriodicalIF":1.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of intestinal parasites in laboratory rats and mice and effectiveness of aqueous extracts from carob pods, Moringa oleifera, and Artemisia alba on the zoonotic parasite Hymenolepis diminuta infection.","authors":"Reham G A Anter, Hoda A Abd-Ellatieff, Hanaa Mohammed, Ahmed Bendari, Rehab E Mohamed, Ashraf Fawzy Mosa Ahmed, Rasha Kamal Elsaid Fahmy, Marwa Alkaffas, Fatima M Ibrahim, Doaa Naguib, Asmaa Aboelabbas Gouda","doi":"10.1007/s11686-025-01062-7","DOIUrl":"https://doi.org/10.1007/s11686-025-01062-7","url":null,"abstract":"","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 4","pages":"141"},"PeriodicalIF":1.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic Analysis of Gene Expression in Immune Pathways in the Spleen of Takifugu rubripes After Infection with Scuticociliate Uronema marinum.","authors":"Qingmeihui Sun, Yanqi Gao, Xiaohang Wang, Gengtong Zhao, Xiuli Wang, Ruijun Li","doi":"10.1007/s11686-025-01076-1","DOIUrl":"https://doi.org/10.1007/s11686-025-01076-1","url":null,"abstract":"","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 4","pages":"140"},"PeriodicalIF":1.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Data on the Endoparasitic Fauna of the Northern White-Breasted Hedgehog Erinaceus roumanicus Barett-Hamilton 1900, in Romania.","authors":"Oana Cristiana Vasiliu, Ioan Liviu Mitrea, Tiberiu C Sahlean, Mariana Ionita","doi":"10.1007/s11686-025-01075-2","DOIUrl":"https://doi.org/10.1007/s11686-025-01075-2","url":null,"abstract":"<p><strong>Purpose: </strong>Despite increasing interest in hedgehog parasitology across Europe, the endoparasitic fauna of the northern white-breasted hedgehog (Erinaceus roumanicus) in Romania remains insufficiently documented. This study aimed to assess the prevalence and diversity of endoparasitic infections and evaluate the influence of age, sex, and habitat on infection patterns.</p><p><strong>Methods: </strong>Between March 2021 and June 2022, data and samples were collected from 135 hedgehogs across Romania, primarily from the Bucharest Metropolitan Area (90/135). Coprological analysis was performed on 97 live individuals admitted to a wildlife rehabilitation centre, using a flotation technique with hyper-saturated sodium chloride solution and the Baermann method. Necropsies were conducted on 38 deceased individuals. Parasites were preserved in 70% ethanol and identified using morphological keys.</p><p><strong>Results: </strong>Endoparasites were detected in 82.47% (80/97) of hedgehogs examined by coproscopy, with mixed infections in 40.21% (39/97). Six parasite species were identified: Crenosoma striatum (61.86%), Capillaria spp. (23.71%), Eucoleus aerophilus (15.46%), Hymenolepis erinacei (14.43%), Brachylaemus erinacei (2.06%), and Cystoisospora rastegaievae (26.80%). Necropsy revealed a helminth prevalence of 76.3% (29/38), with Physaloptera clausa (55.26%) and Nephridiacanthus major (5.26%) found exclusively post-mortem. Statistical analysis of coprological data showed significantly higher C. striatum prevalence in adults (73.68%) compared to juveniles (31.82%), and in males (71.74%) versus females (52.94%) (p < 0.05).</p><p><strong>Conclusion: </strong>This study provides the first comprehensive overview of endoparasites in E. roumanicus in Romania, highlighting notable infection rates and species richness. Results emphasize the influence of age and sex on infection trends, the need for zoonotic risk monitoring, and further research on the potential long-term impacts of parasitism on urban hedgehog populations.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 4","pages":"138"},"PeriodicalIF":1.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Superoxide Dismutase in the Metacestode of Echinococcus granulosus Sensu Stricto (s. s.) and Buffalo Liver.","authors":"Humera Aslam, Monika Rani, Malik Irshadullah","doi":"10.1007/s11686-025-01081-4","DOIUrl":"10.1007/s11686-025-01081-4","url":null,"abstract":"<p><strong>Purpose: </strong>The present study assessed the level of superoxide dismutase (SOD) in the metacestode of Echinococcus granulosus sensu stricto (s. s.) as well as in the liver of Indian water buffalo (Bubalus bubalis).</p><p><strong>Methods: </strong>SOD activity was analyzed by the pyrogallol autoxidation assay and electrophoretic fractionation by native polyacrylamide gel electrophoresis, followed by specific staining of the enzyme. Besides this, metallic co-factor of SOD was determined using different concentrations of inhibitors.</p><p><strong>Results: </strong>The protoscoleces and cyst wall have appreciable amount of SOD, and the level of SOD was found to be significantly (p ≤ 0.001) higher in the fertile compared to non-fertile cyst wall and the infected than non-infected liver. Four SOD activity bands were observed in the protoscoleces, while one band each in the fertile and non-fertile cyst wall, suggesting the occurrence of isozymes in the protoscoleces but not in the cyst wall. Approximately 99% and 16% of enzyme activity were inhibited at 5 mM concentration of KCN and H₂O₂, respectively. Furthermore, no activity band of SOD was detected in the gels treated with 5 mM KCN whereas, the same concentration of H₂O₂ and NaN₃ does not affect the visibility of SOD activity band.</p><p><strong>Conclusion: </strong>The present study indicated that the protoscoleces and cyst wall contain a significant amount of SOD, which likely contributes to their survival within the hostile microenvironment of the host. Inhibition studies revealed the presence of Cu/Zn SOD in the protoscoleces, cyst wall, and host liver. The elevated SOD levels in infected liver may be a protective adaptation against self-generated reactive oxygen species (ROS).</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 4","pages":"137"},"PeriodicalIF":1.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}