Genetic Characteristics of Enterocytozoon bieneusi in Cattle from Hebei Province, China

IF 1.5 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2025-08-07 DOI:10.1007/s11686-025-01114-y

Yuxin Sun, Jing Zhang, Yan Cui, Yan Zhao, Xin Yang, Huizhu Nan, Lei Ma

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引用次数: 0

Abstract

Purpose

Enterocytozoon bieneusi (E. bieneusi) is a microsporidium that causes intestinal disease in animals and humans, and transmits person to person and/or animals to people. A survey was conducted to determine the prevalence and genetic diversity E. bieneusi infection in cattle in Hebei Province, China.

Methods

A total of 2,746 cattle fecal samples were collected from 11 cities in Hebei Province. PCR amplification targeting the ITS gene of E. bieneusi was performed to detect infections. The subtypes, sequence signatures, pairwise genetic distances, and genetic diversity indices were analyzed using MEGA, PhyloSuite, and PopART.

Results

The overall prevalence of E. bieneusi in cattle was 4.70% (129/2746). The infection rate varied significantly by region, age, breeding mode, and cattle type: higher in southern Hebei, cattle under one year old, intensively raised cattle, and dairy cattle. Six genotypes were identified (CHG3, SX1, BEB4, BEB6, BEB8, and J), with BEB6 being the dominant genotype. Sequence analysis revealed 260 polymorphic and conserved sites, including 41 variable sites (15.77% of total nucleotides). The nucleotide diversity index (Pi) was 0.06472, and the haplotype diversity index (Hd) was 1.000.

Conclusion

This study provides the first comprehensive epidemiological data on E. bieneusi infection in cattle in Hebei Province and highlights its rich genetic diversity. These findings offer insights into the potential risk of zoonotic transmission and serve as a reference for further control and prevention strategies.

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河北省牛双氏肠细胞虫的遗传特征。

目的：bieneusenterocytozoon bieneusi （E. bieneusi）是一种引起动物和人类肠道疾病的微孢子虫，并在人与人之间和/或动物之间传播。对河北省牛中布氏伊布氏体感染的流行率和遗传多样性进行了调查。方法：在河北省11个地市采集牛粪标本2746份。采用PCR方法扩增bieneusi ITS基因，检测感染情况。利用MEGA、PhyloSuite和PopART分析其亚型、序列特征、配对遗传距离和遗传多样性指数。结果：牛中总流行率为4.70%（129/2746）。不同地区、年龄、饲养方式、牛种的感染率差异显著：冀南、1岁以下牛、集约化养殖牛、奶牛感染率较高。共鉴定出6个基因型（CHG3、SX1、BEB4、BEB6、BEB8和J），其中BEB6为优势基因型。序列分析发现260个多态性和保守位点，其中41个可变位点（占总核苷酸的15.77%）。核苷酸多样性指数（Pi）为0.06472，单倍型多样性指数（Hd）为1.000。结论：本研究首次提供了河北省牛中布氏伊布氏绦虫感染的综合流行病学资料，并突出了其丰富的遗传多样性。这些发现对人畜共患传播的潜在风险提供了见解，并可作为进一步控制和预防战略的参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.