Acta Parasitologica最新文献

{"title":"Potential Development Ability of Residual Zoites, a Second-Generation Meront, Inducing Long-Term Infection by the Mouse Eimerian Parasite, Eimeria krijgsmanni","authors":"Masanobu Mizuno,&nbsp;Satoru Kiyotake,&nbsp;Makoto Matsubayashi,&nbsp;Takane Kaneko,&nbsp;Hitoshi Hatai,&nbsp;Yoshikazu Fujimoto,&nbsp;Moe Ijiri,&nbsp;Hiroaki Kawaguchi,&nbsp;Toshihiro Matsui,&nbsp;Tomohide Matsuo","doi":"10.1007/s11686-024-00910-2","DOIUrl":"10.1007/s11686-024-00910-2","url":null,"abstract":"<div><h3>Purpose</h3><p>Coccidiosis caused by eimerian parasites results in lethal watery or bloody diarrhea in hosts, and markedly impairs the growth of and feed utilization by host animals. We previously investigated detailed the life cycle of <i>Eimeria krijgsmanni</i> as a mouse eimerian parasite. Only second-generation meronts, as an asexual stage, were morphologically detected in the epithelium of the host cecum for at least 8 weeks after infection, even though oocyst shedding finished approximately 3 weeks after infection. The presence of zoites was of interest because infection by eimerian parasites is considered to be self-limited after their patent period.</p><h3>Methods</h3><p>To clarify the significance of residual second-generation meronts in <i>E</i>. <i>krijgsmanni</i> infection, we performed infection experiments using immunocompetent mice under artificial immunosuppression and congenital immunodeficient mice.</p><h3>Results</h3><p>The number of oocysts discharged and the duration of oocyst discharge both increased in immunosuppressed mice. In immunodeficient mice, numerous oocysts were shed over a markedly longer period, and oocyst discharge did not finish until 56 days after inoculation.</p><h3>Conclusions</h3><p>The present results suggest that the second-generation meronts survived in the epithelial cells of the cecum after the patent period, thereby contributing to extended infection as an asexual stage. The results obtained on <i>E</i>. <i>krijgsmanni</i> indicate that infections by <i>Eimeria</i> spp. are not self-limited and potentially continue for a long period of time.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"1860 - 1865"},"PeriodicalIF":1.2,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-024-00910-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Effects of Papain on Schistosoma mansoni: Miracidial Infection Capacity, Infection Prevalence, Cercarial Shedding and Molecular Changes in Biomphalaria alexandrina","authors":"Amina M. Ibrahim,&nbsp;Sami M. Nasr","doi":"10.1007/s11686-024-00898-9","DOIUrl":"10.1007/s11686-024-00898-9","url":null,"abstract":"<div><h3>Purpose</h3><p>The aim of the present study is to assess the molluscicidal, larvicidal and genotoxicological activities of papain and how it can affect the host-parasite interactions.</p><h3>Methods</h3><p>Toxicity of papain on snails by making series of concentrations to calculate LC₅₀, and then study its larvicide effect on the free larval stages of <i>S. mansoni</i> and infection rate of snails.</p><h3>Results</h3><p>Papain has a molluscicidal activity on adult snails of <i>Biomphalaria alexandrina</i> with a lethal concentration LC₅₀ equals to 43.1 mg/L. In addition, it has activity on miracidia with half Lethal time (LT₅₀) of 16.11 min., and on cercariae with 12.1 min. compared to control ones. The sub lethal concentration LC₁₀ and LC₂₅ (6.9 or 24.1 mg/L, respectively) decreased the survival rate of snails at the first cercarial shedding, the rate of infection, the average total number of cercariae per snail, the shedding period and the life span of snails, while the prepatent period was significantly increased than the control ones. The morphological alterations in cercariae after exposure to papain were occurred where the cercariae lacked motility and some had a dark tail with complete detachment of head and tail. Compared to the control group, the levels of cytochrome oxidase subunit I (COI) and (ND1) genes significantly decreased in snails after exposure to papain.</p><h3>Conclusions</h3><p>Papain could be used as a potential molluscicide for elimination of schistosomiasis and decrease its transmission and deterioration of host-parasite interaction.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"1819 - 1828"},"PeriodicalIF":1.2,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-024-00898-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Leishmania RNA Virus 2 (LRV2) among Clinical Isolates of Leishmania Major in Four Endemic Regions of Iran","authors":"Samaneh Mazaherifar,&nbsp;Saiedeh Erfanian,&nbsp;Kavous Solhjoo,&nbsp;Abazar Roustazadeh,&nbsp;Mohammad Darayesh,&nbsp;Ali Taghipour,&nbsp;Shahab Falahi,&nbsp;Azra Kenarkoohi,&nbsp;Milad Badri,&nbsp;Seyede Manizhe Heidarnejadi,&nbsp;Sima Rasti,&nbsp;Amir Abdoli","doi":"10.1007/s11686-024-00915-x","DOIUrl":"10.1007/s11686-024-00915-x","url":null,"abstract":"<div><h3>Purpose</h3><p><i>Leishmania</i> RNA viruses (LRV) are double-stranded RNA viruses (dsRNA viruses) that play a role in the pathogenesis of <i>Leishmania</i> parasites. Cutaneous leishmaniasis (CL) is endemic in various parts of Iran. Our aimed was to investigate presence of LRV among the <i>Leishmania major</i> isolates in four endemic regions of Iran.</p><h3>Methods</h3><p>In a cross-sectional study, we assessed the presence of LRV1 and LRV2 in 181 clinical isolates of <i>L. major</i> from four endemic cities in Iran using reverse transcription polymerase chain reaction (RT-PCR). After RNA extraction and cDNA synthesis, RT-PCR tests were conducted with LRV1 and LRV2 specific primers. Human beta-actin and <i>kmp</i> genes served as internal and external controls, respectively, and the Allele ID software was used to optimize melting curves.</p><h3>Results</h3><p>LRV2 was detected in 27.6% (50 out of 181) of <i>L. major</i> isolates, while no LRV1 was found. We did not observe a statistically significant difference in the presence of LRV2 based on age group, number, or location of lesions.</p><h3>Conclusion</h3><p>This study confirms the presence of LRV2 in clinical isolates of <i>L. major</i> from endemic regions of Iran. Further researches with larger sample sizes is recommended to explore the association between LRV and clinical symptoms as well as treatment response.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"2046 - 2050"},"PeriodicalIF":1.2,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Echinogammarus lusitanicus (Amphipoda), a New Intermediate Host of Echinorhynchus truttae (Acanthocephala: Echinorhynchidae)","authors":"Seila Couso-Pérez,&nbsp;Alberto Gayoso,&nbsp;Elvira Ares-Mazás,&nbsp;Hipólito Gómez-Couso","doi":"10.1007/s11686-024-00913-z","DOIUrl":"10.1007/s11686-024-00913-z","url":null,"abstract":"<div><h3>Purpose</h3><p>The infection of brown trout (<i>Salmo trutta</i>) by the acanthocephalan parasite <i>Echinorhynchus truttae</i> is initiated by the ingestion of gammarid crustaceans harbouring the cystacanth form. <i>Gammarus pulex</i> has been reported as the common intermediate host of this parasite species. The absence of <i>G. pulex</i> in the rivers of Spain suggests that native gammarid species may play the role of intermediate host for <i>E. truttae</i>, which is the only acanthocephalan species reported in salmonids in this country. The aim of the present study was to investigate whether native gammarid species of Galician rivers can act as intermediate hosts of <i>E. truttae</i>.</p><h3>Methods</h3><p>A total of 263 gammarid specimens were collected from rivers in Galicia and fixed in 70% ethanol. They were examined under stereomicroscope for the presence of acanthocephalan larval stages.</p><h3>Results</h3><p>In one gammarid specimen identified as <i>Echinogammarus lusitanicus</i>, one cystacanth of <i>Echinorhynchus truttae</i> was observed.</p><h3>Conclusion</h3><p>The presence of a cystacanth of <i>E. truttae</i> in the gammarid <i>E. lusitanicus</i> indicates that this species play a role as an intermediate host in the life-cycle of this parasite in Galicia, where <i>G. pulex</i> (the most frequently reported intermediate host of this parasite species) is lacking.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"2043 - 2045"},"PeriodicalIF":1.2,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Species of Creptotrematina (Trematoda: Allocreadiidae) from the Red Minor Tetra, Hyphessobrycon eques (Steindachner, 1882) (Characidae) from Brazil, with Comments on the Genetic Divergence of C. Aguirrepequenoi Jiménez-Guzmán, 1973 across a Wide Geographical Range in Middle America","authors":"Mariana Bertholdi Ebert,&nbsp;David I. Hernández-Mena,&nbsp;Gerardo Pérez-Ponce de León,&nbsp;Reinaldo José da Silva","doi":"10.1007/s11686-024-00877-0","DOIUrl":"10.1007/s11686-024-00877-0","url":null,"abstract":"<div><h3>Background</h3><p>Allocreadiids are relatively small digeneans that appear to be restricted to freshwater systems distributed across the world. Allocreadiids are highly diverse in the Americas, particularly in the Neotropical biogeographical region. Their taxonomic history has been rather controversial, with several taxonomic reassessments and the description of new genera and species.</p><h3>Methods</h3><p>We sampled <i>Creptotrematina</i> specimens from a characid collected in the Pardo River, Paranapanema River basin in Brazil, and specimens of <i>C. aguirrepequenoi</i>, from <i>Astyanax</i> spp. in several localities between northern Mexico and Costa Rica. The specimens were studied through integrative approaches using morphological and molecular analyses of the 28S rDNA gene and two different regions of the COI mtDNA gene.</p><h3>Results</h3><p>We describe a new species of <i>Creptotrematina</i> which is differentiated from other congeners by the overall body size, but in particular by the size and position of the cirrus-sac, distribution of the vitelline follicles, and extension of uterine loops in the posterior end of body. Phylogenetic analyses of the 28S rDNA and COI mtDNA genes placed the new species in a monophyletic clade together with all other sequenced species of <i>Creptotrematina</i>, and as a sister species of <i>C. batalhensis</i>. Genetic divergences between the new species and other <i>Creptotrematina</i> spp. varied from 1.1 to 1.2% for the 28S rDNA and 12.4–14.3% for the COI mtDNA. Phylogenetic analysis based on COI mtDNA showed the isolates of <i>C. aguirrepequenoi</i> grouped in four monophyletic clades representing populations geographically separated along a wide geographical range spanning between northern Mexico and Costa Rica, with an estimated genetic divergence between 3.9% and 8.9%.</p><h3>Conclusions</h3><p>Our findings based on integrative analyses recognize <i>Creptotrematina saltograndensis</i> n. sp. from a characid collected in the Pardo River, Paranapanema River basin in Brazil and provide validation of the wide geographical distribution of <i>C. aguirrepequenoi</i> across Middle-America parasitizing <i>Astyanax</i> spp.; the genetic divergence of the species through the analysis of two regions of COI mtDNA points towards considering it represent a species complex, although we refrain at the moment on describing a new species, awaiting for further verification using other molecular markers, and obtaining fresh material for a more detailed taxonomic analyses. This study increases the known diversity of allocreadiids and contributes to the understanding of evolutionary relationships, host-parasite relationships, and biogeographic history of the group.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"1768 - 1784"},"PeriodicalIF":1.2,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Th1/Th2/Th17 Balance in Pulmonary Cystic Echinococcosis Patients","authors":"Umut Gazi,&nbsp;Yunus Emre Beyhan,&nbsp;Ozgur Tosun,&nbsp;Djursun Karasartova,&nbsp;Ufuk Cobanoglu,&nbsp;Aysegul Taylan-Ozkan","doi":"10.1007/s11686-024-00907-x","DOIUrl":"10.1007/s11686-024-00907-x","url":null,"abstract":"<div><h3>Purpose</h3><p>Cystic echinococcosis (CE) is a neglected tropical disease prevalent worldwide, particularly in rural areas. Previous studies evaluated immune responses in patients with hepatic CE, however none had assessed Th1, Th2 and Th17 levels simultaneously in pulmonary CE patients. This study aimed to fill this gap in literature by using flow cytometry analysis.</p><h3>Methods</h3><p>Peripheral blood mononuclear cells (PBMCs) were isolated from blood samples collected from healthy control (HC) volunteers and patients with active pulmonary CE cysts. The PBMCs were analysed to evaluate Th1, Th2, and Th17 cell levels within the CD3 + CD4 + T-cell population, using antibodies against interferon (IFN)-γ, interleukin (IL)-4, and IL-17, respectively.</p><h3>Results</h3><p>Our analysis revealed elevated Th2 levels in CE patients, while Th1 and Th17 cell counts showed no significant difference between HC volunteers and patients with pulmonary CE.</p><h3>Conclusion</h3><p>The results indicate an imbalanced Th1/Th2/Th17 cell regulation in the pathogenesis of pulmonary CE. Future studies are recommended to compare immune responses between pulmonary and hepatic CE to confirm these findings and evaluate any potential difference in the immunopathology associated with the two clinical forms of CE.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"1829 - 1834"},"PeriodicalIF":1.2,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redescription and Molecular Characterization of the External Attaching Fish Parasitic Cymothoid, Nerocila phaiopleura Bleeker, 1857 (Crustacea: Isopoda) off the Southwest Coast of India","authors":"Amurtha Shyla Suresh,&nbsp;Balamurali Raghavan Pillai Sreekumaran Nair,&nbsp;Binumon Thankachan Mangalathettu,&nbsp;Panakkool Thamban Aneesh","doi":"10.1007/s11686-024-00870-7","DOIUrl":"10.1007/s11686-024-00870-7","url":null,"abstract":"<div><h3>Purpose</h3><p>The identification of the external attaching fish parasitic cymothoid, <i>Nerocila phaiopleura</i> Bleeker 1857, is still based on the brief description of Australian specimens provided by Bruce (1987). The present study aimed to provide a redescription and molecular characterisation of Indian specimens of <i>N. phaiopleura</i>.</p><h3>Materials and Methods</h3><p>Morphological identification was carried out based on microscopic examinations and taxonomic drawings. mitochondrial DNA cox1 was selected as the target gene for sequencing and molecular identification. Nucleotide genetic divergence (p-distance) and base-pair differences among the different species were determined using MEGA11.</p><h3>Results</h3><p><i>Nerocila phaiopleura</i> can be well separated from its congeners by the following combination of characteristics: Body about 2.4 times as long as wide, cephalon broadly rounded anteriorly; coxae posteriorly directed, acute and extending beyond their corresponding pereonite; pereonite 7 posterior angle produced, extending to the pleonite 1; pleonites 1 and 2 ventrolateral process posteriorly directed; uropod exopod straight and elongate, 1.7–2.0 times longer than endopod; uropod endopod lateral margin not serrate, no notch on medial margin; pereopods with short ischium; pleotelson triangular. The p-distance among <i>N. phaiopleura</i> and other available <i>Nerocila</i> spp. ranged from 21 to 19%.</p><h3>Conclusion</h3><p>This study represents the first detailed taxonomic redescription of Indian specimens of <i>N. phaiopleura</i>. Key taxonomic features of the life stages and molecular data are provided here to identify the species properly. Interspecific genetic divergence between <i>N. phaiopleura</i> and other <i>Nerocila</i> spp. is assessed for the first time. Studies in cymothoid life histories, genetics, and morphology are necessary to understand one of the least understood parasite families.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"1749 - 1767"},"PeriodicalIF":1.2,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11686-024-00870-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Molecular Characterization of Clogmia (Telmatoscopus) albipunctatus (Drain Fly) Larva in a Rare Case of Urinary Myiasis in a Domestic Dog in India","authors":"Bindu Lakshmanan,&nbsp;Jacob Alexander,&nbsp;Amrutha Anand,&nbsp;Krishnanunni Nair P. K,&nbsp;Devada K","doi":"10.1007/s11686-024-00881-4","DOIUrl":"10.1007/s11686-024-00881-4","url":null,"abstract":"<div><h3>Purpose</h3><p>The study aimed to identify <i>Clogmia (Telmatoscopus) albipunctata</i> larva recovered from the urinary tract of a four-year-old female Siberian Husky dog in India using morphological assessment and molecular techniques.</p><h3>Methods</h3><p>Larval specimen obtained from the dog urine underwent preliminary morphological assessments followed by polymerase chain reaction (PCR) to selectively amplify segments of the Cytochrome b (Cytb), NADH1 genes, and the tRNA-Ser gene within mitochondrial DNA. Species identification was achieved through sequence analysis of the amplified product and subsequent phylogenetic analysis.</p><h3>Results</h3><p>Larva recovered from dog urine underwent morphological examination, revealing notable features characteristic of <i>Clogmia</i> genus. The primers targeting the mitochondrial DNA gene region of fly larva, amplified a distinct product of approximately 788 bp. Integration of morphological and molecular biology techniques confirmed the identification of the <i>C. albipunctata</i> larva in the dog urinary tract.</p><h3>Conclusion</h3><p>This study elucidates the initial molecular evidence of <i>C. albipunctata</i> presence in a four-year-old female Siberian Husky dog exhibiting symptoms of haematuria and frequent micturition in India. It reveals an instance of a domestic dog in India contracting a <i>C. albipunctata</i> larva infection in its urinary tract, emphasizing the importance of clinical vigilance towards this infection.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"2031 - 2036"},"PeriodicalIF":1.2,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Detection and Characterization of Cryptosporidium spp. and Giardia duodenalis in Dairy Calves from Ningxia, China","authors":"Yue Ma,&nbsp;Xiao-Qing Zan,&nbsp;Ji-Bing Liu,&nbsp;Li-Hua Xu,&nbsp;Hong-Xi Zhao","doi":"10.1007/s11686-024-00914-y","DOIUrl":"10.1007/s11686-024-00914-y","url":null,"abstract":"<div><h3>Purpose</h3><p><i>Cryptosporidium</i> spp. and <i>Giardia duodenalis</i> are two important foodborne human and animal parasites that can be disseminated through both food and water, leading to diarrheal disease. Nevertheless, available information on the circumstances of <i>Cryptosporidium</i> and <i>Giardia duodenalis</i> from Ningxia is limited.</p><h3>Methods</h3><p>A total of 208 stool samples of dairy calves derived from large-scale farms (&gt; 1000 heads) of five cities randomly in Ningxia were gathered randomly, were amplified and analyzed by nested PCR based on the three target genes (18S rRNA, gp60 and tpi)and phylogenetic systematics.</p><h3>Results</h3><p>The prevalence of cryptosporidiosis and giardiasis in dairy calves in Ningxia were 13.0% (27/208 samples, 95% CI 9.1–18.2%) and 1.9% (4/208, 95% CI 0.8–4.9%) respectively. Three <i>Cryptosporidium</i> species appeared in this study which are <i>Cryptosporidium parvum</i> (<i>C. parvum</i>), <i>Cryptosporidium andersoni</i> (<i>C. andersoni</i>) and <i>Cryptosporidium ryanae</i> (<i>C. ryanae</i>) based on the 18S rRNA gene sequence. IIdA15G1 and IIdA13G1 belonging to the subtypes of <i>Cryptosporidium</i> were detected by the <i>gp60</i> PCR. The genotypes of <i>Giardia duodenalis</i> were only assemblage E through the amplification of the triosephosphate-isomerase gene (<i>tpi</i> gene).</p><h3>Conclusion</h3><p>There is a risk of transmission to humans in Ningxia because of zoonotic genotypes (<i>C. parvum, C. andersoni,</i> assemblage E) and subtypes (IId) of <i>Cryptosporidium</i> spp. and <i>G. duodenalis</i> in dairy calves, and it is necessary to pay attention to the disease to prevent a widespread epidemic of the disease with the purpose to protect human and livestock health.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"1876 - 1885"},"PeriodicalIF":1.2,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species of the Genus Paroniella Fuhrmann, 1920 (Cestoda: Davaineidae) from Bulbuls (Passeriformes: Pycnonotidae): Redescription of P. perreti (Mahon, 1954) and Description of P. neolestes n. sp. from Africa","authors":"Yana D. Dimitrova,&nbsp;Gergana P. Vasileva,&nbsp;Boyko B. Georgiev","doi":"10.1007/s11686-024-00908-w","DOIUrl":"10.1007/s11686-024-00908-w","url":null,"abstract":"<div><h3>Purpose</h3><p>The purpose of the present study is to contribute to the knowledge on the diversity of cestodes of the genus <i>Paroniella</i> Fuhrmann, 1920 parasitising passerine birds of the family Pycnonotidae (bulbuls) in the Afrotropical Region. The only known species of this groups, <i>Paroniella perreti</i> (Mahon, 1954) from <i>Pycnonotus tricolor</i> (Hartlaub) in the Democratic Republic of the Congo, is poorly described. Therefore, it needed a detailed redescription in order to make a reliable comparison and provide differentiation among this species and newly-collected davaineid specimens. Based on new materials collected from <i>Neolestes torquatus</i> Cabanis in Gabon, <i>Paroniella neolestes</i> n. sp. is described.</p><h3>Methods</h3><p>The type series of <i>P. perreti</i> from the collection of the Natural History Museum of Geneva is redescribed and figured using conventional light microscopy and interference-contrast microscopy. The specimens of the new species were stained by iron acetocarmine and mounted in Canada balsam.</p><h3>Results</h3><p>The present study provided more detailed and accurate data on <i>P. perreti</i> (compared to its original description) in relation mostly to the armament of suckers (not mentioned in the original description), the structure of the copulative apparatus, the number of rostellar hooks and the number of testes. The morphological comparison of the specimens from <i>Neolestes torquatus</i> from Gabon with the known 53 species of the genus <i>Paroniella</i> (presented in a table format) characterised them as belonging to a new species. The new combination <i>Paroniella oitaensis</i> (Kugi, 1990) n. comb. was proposed.</p><h3>Conclusions</h3><p>Based on the present state of knowledge, <i>P. perreti</i> and <i>P. neolestes</i> seem to be specific each to a single host species, i.e. <i>Pycnonotus tricolor</i> and <i>Neolestes torquatus</i>, respectively. Further studies of davaineid cestodes from pycnonotids from Africa as well as from South and Southeast Asia may result in discovering much greater diversity of this group than currently known.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"1835 - 1851"},"PeriodicalIF":1.2,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142046074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}