{"title":"绵羊和山羊中卵黄杆菌的分子流行、相关危险因素和遗传特征","authors":"Faleh A. AlFaleh","doi":"10.1007/s11686-025-01110-2","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p><i>Theileria</i> species is an important tickborne pathogen of sheep and goats globally. This study was performed in western Saudi Arabia to investigate the prevalence, associated risk factors, and phylogenetic analysis of <i>Theileria ovis</i> (<i>T. ovis)</i> in sheep and goats.</p><h3>Methods</h3><p>A total of 300 blood samples, 190 from sheep and 110 from goats, were collected. The investigation was performed using PCR targeting the small subunit ribosomal RNA (<i>ssu rRNA</i>) gene, in addition to genetic characterization.</p><h3>Results</h3><p>The proportion of goats and sheep testing positive was variable ranging from 9.1 to 13.2%. The total prevalence of <i>T. ovis</i> was found to be 9.1% in goats and 13.2% in sheep. The enrolled sheep and goats were confirmed to be infected with <i>T. ovis</i> by sequencing the partial <i>ssu rRNA</i> gene, and a similar genotype was identified. Age, housing, sex, and tick infestation factors were significantly associated with the infection (<i>p <</i> 0.05). Multivariable model analysis indicated that <i>T. ovis</i> infection was 4.6, 0.4, 3.1, and 8.2 times higher in ages <i>></i> 1 year, females, closed housing, and tick infestation, respectively. Phylogenetic analysis and high nucleotide identities revealed similar genotypes of <i>T. ovis</i> exist in the field, and were close to Turkish <i>T. ovis</i> (OM066225, a goat strain).</p><h3>Conclusions</h3><p>We reported a low to relatively moderate molecular prevalence of <i>T. ovis</i> in goats and sheep, respectively, from the western region. However, it is recommended to adopt control measures to reduce <i>T. ovis</i> infection in small ruminants in this area.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 4","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular Prevalence, Associated Risk Factors, and Genetic Characterization of Theileria Ovis in Sheep and Goats\",\"authors\":\"Faleh A. AlFaleh\",\"doi\":\"10.1007/s11686-025-01110-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p><i>Theileria</i> species is an important tickborne pathogen of sheep and goats globally. This study was performed in western Saudi Arabia to investigate the prevalence, associated risk factors, and phylogenetic analysis of <i>Theileria ovis</i> (<i>T. ovis)</i> in sheep and goats.</p><h3>Methods</h3><p>A total of 300 blood samples, 190 from sheep and 110 from goats, were collected. The investigation was performed using PCR targeting the small subunit ribosomal RNA (<i>ssu rRNA</i>) gene, in addition to genetic characterization.</p><h3>Results</h3><p>The proportion of goats and sheep testing positive was variable ranging from 9.1 to 13.2%. The total prevalence of <i>T. ovis</i> was found to be 9.1% in goats and 13.2% in sheep. The enrolled sheep and goats were confirmed to be infected with <i>T. ovis</i> by sequencing the partial <i>ssu rRNA</i> gene, and a similar genotype was identified. Age, housing, sex, and tick infestation factors were significantly associated with the infection (<i>p <</i> 0.05). Multivariable model analysis indicated that <i>T. ovis</i> infection was 4.6, 0.4, 3.1, and 8.2 times higher in ages <i>></i> 1 year, females, closed housing, and tick infestation, respectively. Phylogenetic analysis and high nucleotide identities revealed similar genotypes of <i>T. ovis</i> exist in the field, and were close to Turkish <i>T. ovis</i> (OM066225, a goat strain).</p><h3>Conclusions</h3><p>We reported a low to relatively moderate molecular prevalence of <i>T. ovis</i> in goats and sheep, respectively, from the western region. However, it is recommended to adopt control measures to reduce <i>T. ovis</i> infection in small ruminants in this area.</p></div>\",\"PeriodicalId\":6932,\"journal\":{\"name\":\"Acta Parasitologica\",\"volume\":\"70 4\",\"pages\":\"\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2025-08-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Parasitologica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11686-025-01110-2\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Parasitologica","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s11686-025-01110-2","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Molecular Prevalence, Associated Risk Factors, and Genetic Characterization of Theileria Ovis in Sheep and Goats
Background
Theileria species is an important tickborne pathogen of sheep and goats globally. This study was performed in western Saudi Arabia to investigate the prevalence, associated risk factors, and phylogenetic analysis of Theileria ovis (T. ovis) in sheep and goats.
Methods
A total of 300 blood samples, 190 from sheep and 110 from goats, were collected. The investigation was performed using PCR targeting the small subunit ribosomal RNA (ssu rRNA) gene, in addition to genetic characterization.
Results
The proportion of goats and sheep testing positive was variable ranging from 9.1 to 13.2%. The total prevalence of T. ovis was found to be 9.1% in goats and 13.2% in sheep. The enrolled sheep and goats were confirmed to be infected with T. ovis by sequencing the partial ssu rRNA gene, and a similar genotype was identified. Age, housing, sex, and tick infestation factors were significantly associated with the infection (p < 0.05). Multivariable model analysis indicated that T. ovis infection was 4.6, 0.4, 3.1, and 8.2 times higher in ages > 1 year, females, closed housing, and tick infestation, respectively. Phylogenetic analysis and high nucleotide identities revealed similar genotypes of T. ovis exist in the field, and were close to Turkish T. ovis (OM066225, a goat strain).
Conclusions
We reported a low to relatively moderate molecular prevalence of T. ovis in goats and sheep, respectively, from the western region. However, it is recommended to adopt control measures to reduce T. ovis infection in small ruminants in this area.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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