The Evolution of the Order Siphonaptera Inferred from the First Mitogenome of the Family Pygiopsyllidae

IF 1.5 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2025-07-04 DOI:10.1007/s11686-025-01051-w

Xiaoxia Lin, Ju Pu, Wenge Dong

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引用次数: 0

Abstract

AbstractSection Purpose

This study presents the first mitogenome sequencing of Aviostivalius klossi bispiniformis and provides a morphological description, while evaluating the phylogenetic position of Pygiopsyllidae within Siphonaptera.

AbstractSection Methods

Morphological identification and imaging of A. k. bispiniformis were performed using a SZ2-ILST dissecting microscope, while its mitogenome was sequenced on the Illumina NovaSeq 6000 high-throughput sequencing platform. Bioinformatics softwares were used to annotate and analyze the structural characteristics and variations of the A. k. bispiniformis. Phylogenetic trees of Siphonaptera were constructed using both Maximum Likelihood (ML) and Bayesian analyses (BI).

AbstractSection Results

The mitogenome of A. k. bispiniformis was 18,669 bp with a high AT content (79.7%). In A. k. bispiniformis, the trnL2 (UAA) gene exhibits a 9-bp anticodon loop, and the nad4-nad4L gene overlapping sequence was 10 bp. Both ML and BI analyses revealed an unstable phylogenetic position of Pygiopsyllidae within Siphonaptera.

AbstractSection Conclusion

In conclusion, available phylogenetic analysis for fleas is subjected to partial taxa data and more taxa data is needed for further researches. This study promotes the understanding of evolution, genomics, and phylogeny of Pygiopsyllidae.

查看原文本刊更多论文

从水蚤科第一有丝分裂基因组推断的虹吸翅目进化。

【摘要】【章节目的】本研究首次对双翼克氏飞蝇（Aviostivalius klossi bispiniformis）进行有丝分裂基因组测序，并对其形态进行描述，同时对其在虹吸翅目中的系统发育地位进行评价。【摘要】方法采用SZ2-ILST解剖显微镜对双尖沙蚕进行形态学鉴定和成像，在Illumina NovaSeq 6000高通量测序平台上对双尖沙蚕进行有丝分裂基因组测序。利用生物信息学软件对双翼沙蚕的结构特征和变异进行了注释和分析。采用最大似然分析（ML）和贝叶斯分析（BI）构建了虹吸翅目的系统发育树。【摘要】【切片】结果双翼沙蚕丝裂基因组全长18669 bp， AT含量高达79.7%。双翼沙蚕trnL2 （UAA）基因有一个9 bp的反密码子环，nad4-nad4L基因重叠序列为10 bp。ML和BI分析均显示该属在虹吸翅目中的系统发育地位不稳定。综上所述，现有的蚤类系统发育分析仅局限于部分类群数据，需要更多的类群数据进行进一步的研究。这一研究促进了人们对Pygiopsyllidae的进化、基因组学和系统发育的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.