Journal of Consumer Protection and Food Safety最新文献

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Meta-analysis of the prevalence of non-typhoidal Salmonella (NTS) in layer poultry eggs and feces in India 印度蛋鸡蛋和粪便中非伤寒沙门氏菌流行情况的荟萃分析
IF 1.7 3区 农林科学
Journal of Consumer Protection and Food Safety Pub Date : 2026-02-07 DOI: 10.1007/s00003-026-01599-3
Kushal Grakh, Vijay Jayawant Jadhav, Vinay Ganeshrao Joshi, Rajesh Khurana, Naresh Jindal
{"title":"Meta-analysis of the prevalence of non-typhoidal Salmonella (NTS) in layer poultry eggs and feces in India","authors":"Kushal Grakh,&nbsp;Vijay Jayawant Jadhav,&nbsp;Vinay Ganeshrao Joshi,&nbsp;Rajesh Khurana,&nbsp;Naresh Jindal","doi":"10.1007/s00003-026-01599-3","DOIUrl":"10.1007/s00003-026-01599-3","url":null,"abstract":"<div>\u0000 \u0000 <p>The rising production and consumption of eggs, coupled with the increased movement of people, birds, and feed, have heightened the threat of <i>Salmonella</i> infections in several countries, including India. This study was undertaken to estimate the prevalence of non-typhoidal <i>Salmonella</i> in poultry eggs (surface and content) and feces (cloacal, caecal, and fecal swabs) in India using meta-analytical methods. Analysis revealed that out of 5,050 samples processed across the included studies, the pooled prevalence of non-typhoidal <i>Salmonella</i> was 4.4%. Both Egger’s regression asymmetry test (<i>p</i> value = 0.343) and the funnel plot indicated no evidence of publication bias or small-study effects. Subgroup meta-analysis found a significantly higher occurrence of <i>Salmonella</i> in feces (8%) compared to egg surface (4%) and egg content (2%) samples. <i>S</i>. Typhimurium was the dominant serovar (70/218), followed by <i>S</i>. Enteritidis (56/218). Other major serovars included <i>S</i>. Worthington, <i>S</i>. Bareilly, <i>S</i>. Gallinarum, and <i>S</i>. Dublin. While all the studies employed serotyping for identification, 7 also used PCR, with 3 of these targeting fimbrial genes for serotype detection. Most non-typhoidal <i>Salmonella</i> serovars possess zoonotic potential and pose a significant risk to public health. Implementing safety measures at poultry farms, egg processing facilities, and retail outlets—alongside educating roadside vendors and consumers—could substantially reduce the risk of <i>Salmonella</i> transmission to humans.</p>\u0000 </div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"21 2","pages":"127 - 137"},"PeriodicalIF":1.7,"publicationDate":"2026-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148060535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Amtliche Mitteilungen 官方新闻稿
IF 1.7 3区 农林科学
Journal of Consumer Protection and Food Safety Pub Date : 2026-02-05 DOI: 10.1007/s00003-026-01598-4
{"title":"Amtliche Mitteilungen","authors":"","doi":"10.1007/s00003-026-01598-4","DOIUrl":"10.1007/s00003-026-01598-4","url":null,"abstract":"","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"21 1","pages":"119 - 120"},"PeriodicalIF":1.7,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147579094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction: Dissipation of six insecticides and evaluation of their processing factors in different chilli samples 更正:6种杀虫剂在不同辣椒样品中的耗散及处理因素评价
IF 1.7 3区 农林科学
Journal of Consumer Protection and Food Safety Pub Date : 2026-01-30 DOI: 10.1007/s00003-026-01596-6
Jay V. Italiya, Ravi L. Kalasariya, Suchi Chawla, Paresh H. Rathod, R. R. Acharya
{"title":"Correction: Dissipation of six insecticides and evaluation of their processing factors in different chilli samples","authors":"Jay V. Italiya,&nbsp;Ravi L. Kalasariya,&nbsp;Suchi Chawla,&nbsp;Paresh H. Rathod,&nbsp;R. R. Acharya","doi":"10.1007/s00003-026-01596-6","DOIUrl":"10.1007/s00003-026-01596-6","url":null,"abstract":"","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"21 1","pages":"123 - 123"},"PeriodicalIF":1.7,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147579558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Beyond fixed pre-harvest intervals: a statistical analysis supporting BBCH-based pesticide late application timing in cereals in Europe 超越固定的收获前间隔:一项支持欧洲谷物中基于bbch的农药晚施用时间的统计分析
IF 1.7 3区 农林科学
Journal of Consumer Protection and Food Safety Pub Date : 2026-01-15 DOI: 10.1007/s00003-025-01594-0
Adeline Mousques, Christian J. Kuster, Luke Settles, Oliver Schaudt
{"title":"Beyond fixed pre-harvest intervals: a statistical analysis supporting BBCH-based pesticide late application timing in cereals in Europe","authors":"Adeline Mousques,&nbsp;Christian J. Kuster,&nbsp;Luke Settles,&nbsp;Oliver Schaudt","doi":"10.1007/s00003-025-01594-0","DOIUrl":"10.1007/s00003-025-01594-0","url":null,"abstract":"<div><p>Plant protection product applications in cereals require careful timing to ensure food safety while maintaining efficacy. European regulatory frameworks employ 2 approaches for flowering applications: (a) fixing a growth stage for the last application (BBCH scale) or (b) establishing a fixed pre-harvest interval (PHI), expressed as days before harvest. Understanding the relationship between these approaches is crucial for designing effective supervised residue trials and ensuring reliable residue assessments. This case study uses residue trial data from fungicide applications at growth stages BBCH 61 and 69 in barley and wheat respectively. We evaluated how well-fixed PHI requirements align with natural crop development patterns across European cereal production regions. Results reveal a significant mismatch between fixed PHI of 35 days (window acceptance 26–44 days) and biological crop development, with only 34–50% of trials achieving regulatory compliance depending on crop and region. This biological-temporal disconnect necessitates conducting 15–35 trials to achieve a complete residue package of eight valid trials with reasonable confidence. When crops develop more slowly than prescribed PHIs, stakeholders face the dilemma of collecting immature samples (contradicting the “as low as reasonably achievable” principle) or risking trial invalidation. The consistency of these patterns across Europe indicates the challenge stems from imposing fixed intervals on inherently variable biological processes rather than regional variations. Based on these scientific findings, we propose transitioning to purely BBCH-based label instructions for flowering cereal applications to better align with agronomic reality, improve farmer compliance, and provide more accurate dietary exposure assessments for consumers.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"21 1","pages":"53 - 60"},"PeriodicalIF":1.7,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-025-01594-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147579164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Residues, decontamination, processing factors and risk assessment of organophosphorus insecticides in cauliflower 菜花中有机磷杀虫剂残留、去污、加工因素及风险评价
IF 1.7 3区 农林科学
Journal of Consumer Protection and Food Safety Pub Date : 2026-01-09 DOI: 10.1007/s00003-025-01595-z
Anju Padmanabhan, M. Paramasivam, N. Muthukrishnan, K. Bhuvaneswari
{"title":"Residues, decontamination, processing factors and risk assessment of organophosphorus insecticides in cauliflower","authors":"Anju Padmanabhan,&nbsp;M. Paramasivam,&nbsp;N. Muthukrishnan,&nbsp;K. Bhuvaneswari","doi":"10.1007/s00003-025-01595-z","DOIUrl":"10.1007/s00003-025-01595-z","url":null,"abstract":"<div><p>Residue studies and dietary risk assessments are essential for evaluating the safe use of pesticides in crops. In the present study, a Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) extraction coupled with gas chromatography-flame photometric detection was developed to quantify chlorpyrifos, ethion, and triazophos residues in cauliflower. In addition, field trials were conducted to investigate the dissipation and residue behaviour of chlorpyrifos, ethion, and triazophos in cauliflower. The dietary risk for Indian consumers was evaluated using risk quotient methods. The average recoveries of chlorpyrifos, ethion, and triazophos ranged from 103 to 115%, 103 to 114%, and 96 to 99%, respectively, with a relative standard deviation (RSD) of &lt; 5%. Chlorpyrifos, ethion, and triazophos residues dissipated with half-lives of 2.8, 4.3, and 2.7 days, respectively. Washing the cauliflower, followed by boiling decreased the residues by over 70%, resulting in a processing factor of &lt; 1. Chronic risk quotients (RQs) were &lt; 1, except for triazophos on the first day after application, indicating that consumption of cauliflower was safe for all age groups of Indian consumers. The pre-harvest intervals required to ensure compliance with the maximum residue limit (MRL) for chlorpyrifos, ethion, and triazophos in cauliflower were 3, 10, and 24 days, respectively.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"21 1","pages":"75 - 85"},"PeriodicalIF":1.7,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147579307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhancing microbial food safety in Indonesia’s artisanal cheese sector: A strategic framework for consumer protection 加强印尼手工奶酪行业的微生物食品安全:保护消费者的战略框架
IF 2.2 3区 农林科学
Journal of Consumer Protection and Food Safety Pub Date : 2026-01-07 DOI: 10.1007/s00003-025-01592-2
Nodali Ndraha, Ema Damayanti, Andri Frediansyah, Vertileni Ndraha, Siska Aditya
{"title":"Enhancing microbial food safety in Indonesia’s artisanal cheese sector: A strategic framework for consumer protection","authors":"Nodali Ndraha,&nbsp;Ema Damayanti,&nbsp;Andri Frediansyah,&nbsp;Vertileni Ndraha,&nbsp;Siska Aditya","doi":"10.1007/s00003-025-01592-2","DOIUrl":"10.1007/s00003-025-01592-2","url":null,"abstract":"<div>\u0000 \u0000 <p>Artisanal cheese production, a high-value component of global culinary heritage, presents significant public health risks when operating without rigorous safety controls. This challenge is particularly acute in emerging markets like Indonesia, where the sector’s rapid expansion is outpacing regulatory development, thereby creating a direct threat to consumer protection. Here, we systematically review global and regional data on the prevalence of key pathogens—such as <i>Listeria monocytogenes</i>, <i>Salmonella</i> spp., Shiga toxin-producing <i>E. coli</i> (STEC), and <i>Staphylococcus aureus—</i>and emerging threats, including <i>Coxiella burnetii</i>, in artisanal cheeses. We apply these findings to Indonesian context to develop a framework for mitigation strategies that prioritizes consumer protection and food safety. The review found that pathogen prevalence in raw milk artisanal cheeses globally is highly variable, with very high rates in informal markets. These risks are exacerbated in Indonesia due to poor raw milk hygiene, inadequate cold chain infrastructure, unvalidated cheese-making and ripening practices, and gaps in microbial surveillance. These vulnerabilities are further compounded by emerging threats from climate change, antimicrobial resistance, and mycotoxin contamination. In response, we propose a comprehensive framework to improve safety, strengthen consumer protection, and ensure sustainable sector growth. Key strategies include standardized hygiene protocols from farm to final product, adoption of accessible technologies, validation of ripening processes to ensure pathogen inactivation, and evidence-based regulations specifically designed for small-scale producers. Implementation of this framework is intended to safeguard public health, build consumer trust, and ensure the long-term viability of Indonesia’s artisanal cheese, offering a model for other nations facing similar food safety challenges.</p>\u0000 </div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"21 3","pages":"247 - 255"},"PeriodicalIF":2.2,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148903264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Toxicological evaluation of a fungal-derived natural colorant using Caenorhabditis elegans: implications for food safety 使用秀丽隐杆线虫的真菌衍生天然着色剂的毒理学评价:对食品安全的影响
IF 1.7 3区 农林科学
Journal of Consumer Protection and Food Safety Pub Date : 2025-12-05 DOI: 10.1007/s00003-025-01590-4
Talita Cristina Mena Segato, Francisco Javier Novoa San Miguel, Erika Leão Ajala Caetano, Vicente André Araya Salcedo, Caio de Azevedo Lima, Valéria Carvalho Santos-Ebinuma, Maria Fernanda Hornos Carneiro, Denise Grotto
{"title":"Toxicological evaluation of a fungal-derived natural colorant using Caenorhabditis elegans: implications for food safety","authors":"Talita Cristina Mena Segato,&nbsp;Francisco Javier Novoa San Miguel,&nbsp;Erika Leão Ajala Caetano,&nbsp;Vicente André Araya Salcedo,&nbsp;Caio de Azevedo Lima,&nbsp;Valéria Carvalho Santos-Ebinuma,&nbsp;Maria Fernanda Hornos Carneiro,&nbsp;Denise Grotto","doi":"10.1007/s00003-025-01590-4","DOIUrl":"10.1007/s00003-025-01590-4","url":null,"abstract":"<div><p>The growing demand for natural colorants as an alternative to synthetic dyes has raised the need for robust safety assessments to support their regulatory approval and safe use in food products. This study evaluated the toxicological profile of a novel azaphilone pigment produced by <i>Talaromyces amestolkiae</i> using <i>Caenorhabditis elegans</i> as a validated <i>in vivo</i> alternative model. Synchronized L1-stage nematodes were exposed for 72 h to azaphilone-contaminated <i>Escherichia coli</i> at concentrations ranging from 3.1 to 50%. Toxicological endpoints included body length, motility, intra-uterine egg count, reproductive performance, larval lethality, and lifespan. Exposure to the colorant led to significant increases in worm length and motility, induced larval lethality, and reduced lifespan at the lowest concentration tested. No mortality was observed, but the effects were sufficient to preclude the identification of a No Observed Adverse Effect Level (NOAEL). A Low Observed Adverse Effect Level (LOAEL) was established below 3.1%. The presence of glucose (1.54 g/100 mL) in the extract may have contributed to some of the observed effects, reflecting the need for improved production processes. The concentrations tested exceeded typical food-use levels. The present study provides important preliminary data to guide future risk assessments. In conclusion, our findings demonstrate that while the azaphilone pigment from <i>Talaromyces amestolkiae</i> shows biological activity at high concentrations, its safety at realistic dietary exposure levels warrants further investigation.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"21 1","pages":"105 - 113"},"PeriodicalIF":1.7,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147579643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of hygienic conditions and bacteria of public health relevance on abiotic surfaces and cleaning tools in Greek university cafeterias 希腊大学食堂非生物表面和清洁工具的卫生条件和与公共卫生相关的细菌评估
IF 1.7 3区 农林科学
Journal of Consumer Protection and Food Safety Pub Date : 2025-12-05 DOI: 10.1007/s00003-025-01591-3
Thomai Lazou, Serafeim Chaintoutis
{"title":"Assessment of hygienic conditions and bacteria of public health relevance on abiotic surfaces and cleaning tools in Greek university cafeterias","authors":"Thomai Lazou,&nbsp;Serafeim Chaintoutis","doi":"10.1007/s00003-025-01591-3","DOIUrl":"10.1007/s00003-025-01591-3","url":null,"abstract":"<div><p>This study aimed to evaluate the hygienic conditions and the presence and antimicrobial susceptibility of foodborne pathogens and indicator organisms in environmental samples from 15 out of 18 (83%) operating cafeterias at public universities in Northern Greece. A checklist with 7 dichotomous (yes/no) evaluation criteria was used to assess the observed hygienic conditions. One dish sponge and one kitchen cloth were aseptically collected, and 5 types of abiotic surfaces were sampled with sterile sponges (refrigerator door handle, food serving tray, food display refrigerator shelf, kitchen countertop, table) per cafeteria. All environmental samples (n = 105) were analysed according to microbiological ISO standard methods. VITEK<sup>®</sup> 2 was used for the identification and antimicrobial susceptibility testing (AST) of bacterial isolates. Overall, satisfactory hygienic conditions were observed, but deviations were also detected in the form of visibly unclean cloths (13%), lack of hot tap water (27%), and refrigerators operating above 5 °C (87%). No <i>Campylobacter</i>, <i>Salmonella</i>, <i>Listeria monocytogenes,</i> or <i>Escherichia coli</i> were detected<i>.</i> However, the samples yielded <i>Staphylococcus aureus</i> (7%), coagulase-negative staphylococci (CoNS) (33%), and coliforms (18%) at levels exceeding the available recommended thresholds. Eight CoNS species were identified, which exhibited 12 diverse antimicrobial resistance phenotypes of public health interest, mainly against beta-lactams, macrolides, and lincosamides, and to a lesser extent against aminoglycosides and tetracyclines. This study provides the most extensive available report on the concurrent detection, species identification, and AST of CoNS isolated from food service environments. The results underscore the need for targeted environmental monitoring in food-handling areas including the detection of bacteria with antimicrobial resistance that could pose a risk to public health.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"21 2","pages":"197 - 205"},"PeriodicalIF":1.7,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148060837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Practical application of BREAM2 in bystander/resident spray drift exposure assessments: accounting for variations in water volume BREAM2在旁观者/居民喷雾漂移暴露评估中的实际应用:考虑水量的变化
IF 1.7 3区 农林科学
Journal of Consumer Protection and Food Safety Pub Date : 2025-11-22 DOI: 10.1007/s00003-025-01589-x
Kathrin Bürling, Edgars Felkers, Christian J. Kuster, Siân L. Wright-Williams, M. Clare Butler Ellis
{"title":"Practical application of BREAM2 in bystander/resident spray drift exposure assessments: accounting for variations in water volume","authors":"Kathrin Bürling,&nbsp;Edgars Felkers,&nbsp;Christian J. Kuster,&nbsp;Siân L. Wright-Williams,&nbsp;M. Clare Butler Ellis","doi":"10.1007/s00003-025-01589-x","DOIUrl":"10.1007/s00003-025-01589-x","url":null,"abstract":"<div><p>The EFSA 2022 guidance on non-dietary human exposure, including operators, workers, residents and bystanders, relies on output from the original BREAM model, including simplified extrapolation for different application volumes. This study aims to present the current limitations and challenges in the European Plant Protection Product (PPP) authorizations for non-dietary exposure and risk assessments of residents and bystanders. It also and outlines a concept that could help overcome these limitations while maintaining a health-protective approach. The study demonstrates that refining the value of spray liquid (mL/ person) by more reliably incorporating application volume as a variable in the model leads to a more realistic and scientifically sound risk assessment. This refined approach has been shown to be both reasonable and applicable in the context of PPP registration processes, ahead of its anticipated inclusion in the next EFSA guidance update, where the implementation of BREAM2 is expected.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"21 1","pages":"3 - 10"},"PeriodicalIF":1.7,"publicationDate":"2025-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-025-01589-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147579150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Retrieving sequences of genetically modified plants from public databases to widen screening approaches 从公共数据库中检索转基因植物序列以扩大筛选方法
IF 1.7 3区 农林科学
Journal of Consumer Protection and Food Safety Pub Date : 2025-11-20 DOI: 10.1007/s00003-025-01581-5
M. Colaiacovo, L. Bonfini, M. Maretti, M. Petrillo, C. Savini
{"title":"Retrieving sequences of genetically modified plants from public databases to widen screening approaches","authors":"M. Colaiacovo,&nbsp;L. Bonfini,&nbsp;M. Maretti,&nbsp;M. Petrillo,&nbsp;C. Savini","doi":"10.1007/s00003-025-01581-5","DOIUrl":"10.1007/s00003-025-01581-5","url":null,"abstract":"<div><p>The development of effective screening methods and implementation of official controls on genetically modified organisms (GMOs) relies heavily on the availability of comprehensive and reliable GMO sequence repositories. Existing GMO databases, including the one developed by the Joint Research Centre (JRC), do not cover all events authorised worldwide. To bridge this gap, we designed a bioinformatics pipeline to retrieve new GMO sequences from public databases. We used for our initial query a 25 bp Ti plasmid sequence, which is commonly retained in the host genome after <i>Agrobacterium tumefaciens</i>-mediated transformation. Analysis of the initial dataset enabled us to identify longer query sequences, subsequently used to retrieve additional GMO sequences. This approach yielded the complete sequences of 23 GMOs. The bioinformatics strategy can be extended to other genetic targets frequently used in GMO development, facilitating the design of more effective screening approaches. As a proof of concept, we developed a new real-time PCR screening method targeting the <i>Agrobacterium</i>-derived region, using common sequences identified in our bioinformatics analysis. The resulting method demonstrated good sensitivity and specificity, successfully detecting 13 unauthorised GMOs in the European Union that were developed using <i>Agrobacterium</i>-mediated transformation.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"21 1","pages":"41 - 51"},"PeriodicalIF":1.7,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-025-01581-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147579540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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