Journal of Consumer Protection and Food Safety最新文献

{"title":"Gram-negative bacterial diversity and antimicrobial resistance patterns in fish fillets from a seafood market in Brazil","authors":"Fernanda Freitas Dominguez,&nbsp;Felipe Vásquez-Ponce,&nbsp;Johana Becerra,&nbsp;Jessica Bordin,&nbsp;Fábio Parra Sellera,&nbsp;Nilton Lincopan,&nbsp;Marcelo Barbosa Henriques","doi":"10.1007/s00003-025-01551-x","DOIUrl":"10.1007/s00003-025-01551-x","url":null,"abstract":"<div><p>Fish and seafood can harbor pathogenic Gram-negative bacteria, posing health risks to consumers. These bacteria, known for causing severe gastrointestinal diseases, may also carry antimicrobial resistance (AMR) genes. This study investigated the occurrence and AMR patterns of Gram-negative bacteria in raw fish fillets from a Brazilian seafood market, based on a limited sample of species commonly consumed raw, including trout, tilapia, and pangasius. Muscle samples were analyzed using microbiological methods, including isolation on selective media supplemented with antimicrobials. Bacterial species identification was conducted using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS), while antimicrobial susceptibility testing was performed using the disk diffusion method. A diverse array of bacterial species was detected in 28 colonies selected for identification, including <i>Aeromonas veronii</i> (60.71%), <i>Morganella morganii</i> (17.85%) <i>Pseudomonas otitidis</i> (7.14%), <i>Acinetobacter baumannii</i> (3.57%), <i>Aeromonas jandaei</i> (3.57%), and <i>Citrobacter braakii</i> (3.57%). Antimicrobial susceptibility testing indicated varying resistance profiles. Excluding intrinsic resistance, 50% of the isolates were resistant to amoxicillin + clavulanic acid, 7.14% to ceftriaxone, 7.14% to chloramphenicol, and 3.57% to cefotaxime, with no multidrug resistance detected. All fish originated from aquaculture, contamination may stem from antimicrobial use or mishandling during transportation and storage. These findings highlight the need for strengthened monitoring and management strategies to ensure seafood safety, particularly for raw-consumed species popular in Japanese cuisine.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"20 2","pages":"175 - 180"},"PeriodicalIF":1.7,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145168314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probabilistic and deterministic risk assessment of chloramphenicol residues in broiler chicken meat","authors":"Davit Pipoyan,&nbsp;Meline Beglaryan,&nbsp;Viktoriia Chirkova,&nbsp;Alberto Mantovani","doi":"10.1007/s00003-025-01549-5","DOIUrl":"10.1007/s00003-025-01549-5","url":null,"abstract":"<div><p>This study is the first in the Caucasus region to investigate the presence of chloramphenicol (CAP) residues in broiler chicken meat sold in Yerevan, Armenia, and to assess potential health risks. A food frequency questionnaire was distributed among 1,040 adults in Yerevan to determine chicken consumption patterns. Respondents were then classified into 3 clusters using K-means clustering. 30 broiler chicken meat samples, including 18 local and 12 imported products, were collected and analyzed for CAP residues using Enzyme-Linked Immunosorbent Assay (ELISA) and High-Performance Liquid Chromatography (HPLC). Potential health risks were evaluated by calculating Daily Intake (DI) and Margin of Exposure (MOE) for each consumer cluster, using both deterministic and probabilistic approaches. Due to CAP genotoxicity, an MOE below 10,000 was considered indicative of a potential health concern. CAP residues were detected in 60% of the broiler chicken meat samples, with higher detection rates and concentrations in imported products compared to local ones. Both deterministic and probabilistic analyses, including Monte Carlo simulations, indicated that CAP residues in chicken meat is unlikely to pose a health risk to the studied population. While exposure through chicken meat alone does not raise immediate concern, available literature indicates that CAP exposure may also occur through the consumption of other food products, calling for a comprehensive risk assessment. These findings highlight the need of stricter monitoring and regulation of CAP residues from farm to market, especially in countries like Armenia, where chicken meat is a major dietary component. The study also emphasizes the importance of using comprehensive risk assessment approaches for veterinary drug residues, also by combining deterministic and probabilistic methods.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"20 2","pages":"155 - 164"},"PeriodicalIF":1.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145167359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"94. Arbeitstagung des ALTS","authors":"","doi":"10.1007/s00003-025-01548-6","DOIUrl":"10.1007/s00003-025-01548-6","url":null,"abstract":"","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"20 2","pages":"199 - 205"},"PeriodicalIF":1.7,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145165588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pressure-assisted isolation of micro- and nanoplastics from food of animal origin with special emphasis on seafood","authors":"Julia Süssmann,&nbsp;Elke Walz,&nbsp;Birgit Hetzer,&nbsp;Ralf Greiner,&nbsp;Elke Kerstin Fischer,&nbsp;Sascha Rohn,&nbsp;Jan Fritsche","doi":"10.1007/s00003-025-01543-x","DOIUrl":"10.1007/s00003-025-01543-x","url":null,"abstract":"<div><p>Recent studies have indicated the presence of micro- and nanoplastics in food of animal origin. To address the concerns regarding human dietary plastic uptake, extensive monitoring using validated analytical methods is required. However, a major challenge in analysis lies in the high complexity of preparing food samples rich in macromolecular content (e.g., proteins, complex lipids), such as seafood. Consequently, this study aimed to optimise sample preparation methods for seafood, meat, milk, honey, and eggs, ensuring negligible impact on plastic integrity. Sample matrices were digested using combinations of alkaline, oxidative, and enzymatic approaches, with incubation periods ranging from 6 to 16 h. Particles were rapidly isolated by pressure-assisted filtration, which retained particles in the lower micron range. This method, used for isolating microplastic from food, was compared to the commonly employed vacuum filtration technique. Pressure-assisted filtration improved filtration rates and matrix removal while demonstrating comparable impact on procedural contamination and polymer integrity to vacuum filtration. The feasibility of microplastic analysis was demonstrated using fluorescence imaging. In contrast to microplastics, nanoplastics (&lt; 1 µm) have greater potential for negative biological effects due to their small size, warranting detailed analysis. However, their low particle mass, susceptibility for agglomeration, and the detection limits of conventional spectroscopic techniques pose unique analytical challenges. Addressing these challenges is essential for developing standardised methodologies, including certified food reference material, to enable safety assessments of micro- and nanoplastic in food.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"20 2","pages":"141 - 154"},"PeriodicalIF":1.7,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-025-01543-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145163356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Climate change impacts on livestock and resulting effects on animal health: current challenges in food safety, consumer protection, and animal welfare","authors":"Diego Antonio Sicuso,&nbsp;Annalisa Previti,&nbsp;Michela Pugliese,&nbsp;Annamaria Passantino","doi":"10.1007/s00003-025-01544-w","DOIUrl":"10.1007/s00003-025-01544-w","url":null,"abstract":"","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"20 1","pages":"1 - 3"},"PeriodicalIF":1.4,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upcoming events","authors":"","doi":"10.1007/s00003-025-01545-9","DOIUrl":"10.1007/s00003-025-01545-9","url":null,"abstract":"","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"20 1","pages":"111 - 113"},"PeriodicalIF":1.4,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current status and trends in the analysis of GMO and new genomic techniques","authors":"Hermann Broll,&nbsp;Joachim Bendiek,&nbsp;Albert Braeuning,&nbsp;Kolja Neil Eckermann,&nbsp;Anuscha Gebhardt,&nbsp;Lutz Grohmann,&nbsp;Nina Keiss,&nbsp;Jörn Lämke,&nbsp;Joachim Mankertz,&nbsp;Werner Schenkel,&nbsp;Ursula Vincent,&nbsp;Christopher Weidner,&nbsp;Maddalena Querci","doi":"10.1007/s00003-025-01542-y","DOIUrl":"10.1007/s00003-025-01542-y","url":null,"abstract":"<div><p>The detection of genetically modified organisms (GMO) is crucial for regulatory compliance and market control. Therefore, detection methods are constantly being developed and improved. New genomic techniques (NGT) present unique challenges for detection due to the minimal genetic changes they introduce. The ‘International Conference on GMO Analysis and New Genomic Techniques’ held in March 2023 in Berlin, explored advances in detection methods, such as Next-Generation Sequencing and digital PCR, and highlighted the need for global cooperation and capacity-building initiatives. The conference emphasized international collaboration and addressed the challenges of establishing robust methods for identifying NGT-derived organisms.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"20 1","pages":"89 - 92"},"PeriodicalIF":1.4,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-025-01542-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colchicine as a food contaminant: rare occurrence but persistent in stored honey and during yogurt fermentation","authors":"Florian Kaltner,&nbsp;Gerd Hamscher","doi":"10.1007/s00003-025-01541-z","DOIUrl":"10.1007/s00003-025-01541-z","url":null,"abstract":"<div><p>Colchicine, a plant toxin with aneugenic and potentially genotoxic properties, is predominately derived from the autumn crocus (<i>Colchicum autumnale</i>). Although it has been used as a drug since ancient times, severe poisoning or even death may occur in humans and animals when colchicine is ingested in larger amounts. If consumed by food-producing animals, the transfer of colchicine to animal-based food products, as observed with other plant toxins, seems likely, posing a potential health risk to consumers. In the late flowering period of <i>C. autumnale</i>, honey bees may have limited alternatives and may collect its nectar and pollen, potentially transferring colchicine to honey. A literature research on the relevance of colchicine as food contaminant was conducted, followed by experiments to examine its stability in stored honey and milk fermented into yoghurt. The literature review revealed that colchicine is rarely detected as a food contaminant. However, it has been shown to transfer into milk of ruminants following the ingestion of <i>C. autumnale</i>. Experiments with artificially contaminated samples demonstrated, for the first time, the persistence of colchicine in honey stored for 4 weeks under dark conditions and during the fermentation of milk into yoghurt. Although the overall risk to consumers currently appears low, further research is needed to determine whether the continued spread of <i>C. autumnale</i> poses a higher risk to livestock and consumers than previously assumed.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"20 1","pages":"81 - 87"},"PeriodicalIF":1.4,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-025-01541-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and in-house validation of two real-time PCR methods for the detection of genome-editing events in soybean FAD2 gene variants","authors":"Steffen Heinz,&nbsp;Daniel Neusius,&nbsp;Kolja Neil Eckermann,&nbsp;Klaus Pietsch,&nbsp;Patrick Guertler","doi":"10.1007/s00003-024-01538-0","DOIUrl":"10.1007/s00003-024-01538-0","url":null,"abstract":"<div><p>Since the commercialization of the first genetically modified (GM) crops, their relevance in agricultural applications has increased. Soybean (<i>Glycine max</i>) ranks as the most widely cultivated GM crop globally. With the advancements in site-specific genome-editing tools, the development of GM organisms (GMO) using new genomic techniques has accelerated, particularly in agriculture. In the US, a genome-edited soybean variety, Calyno, was developed with altered fatty acid composition by inactivating two members of the <i>fatty acid desaturase 2</i> (<i>FAD2</i>) gene family, <i>FAD2-1A</i> and <i>FAD2-1B</i>, using TALEN technology. Although deregulated in the US, Calyno soybeans are not authorized for cultivation or import in the European Union and therefore cannot be placed on the European market. To address the need for reliable detection methods in routine analysis for GMO by public authorities, two event-specific qPCR methods were developed to detect the <i>FAD2-1A-Δ63bp</i> and <i>FAD2-1B-Δ23bp</i> gene variants in the Calyno soybeans. In the absence of reference material, methods were validated in-house using synthetic plasmids carrying the target regions’ sequences, following European Network of GMO Laboratories (ENGL) guidelines. Both assays meet the minimum performance requirements for GMO testing, demonstrating high sensitivity, qPCR efficiency, specificity and robustness. An interlaboratory comparison study further confirmed the reliability of these methods. These qPCR assays provide an effective tool for detecting the <i>FAD2-1A-Δ63bp</i> and <i>FAD2-1B-Δ23bp</i> gene variants in Calyno soybeans, supporting GMO testing requirements in the European Union and ensuring accurate monitoring of GM crops.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"20 1","pages":"53 - 62"},"PeriodicalIF":1.4,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-024-01538-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Food wastage along the global food supply chain and the impact of food packaging","authors":"Elisa Uhlig,&nbsp;Anna Sadzik,&nbsp;Mara Strenger,&nbsp;Anna-Maria Schneider,&nbsp;Markus Schmid","doi":"10.1007/s00003-024-01539-z","DOIUrl":"10.1007/s00003-024-01539-z","url":null,"abstract":"<div><p>Inadequate food handling throughout the food supply chain can result in food loss and waste, leading to significant environmental, economic and social impacts. Identifying the causes of food loss and waste across the food supply chain, including the role of packaging, is critical for developing reduction and prevention strategies. This review examines 54 scientific studies to identify packaging-related causes of food loss and waste, evaluate whether and how packaging contributes to or mitigates these issues, and propose measures to address them. The findings highlight the need for standardized terminology and methodologies to improve comparability among studies. Key packaging-related issues identified include difficulty emptying, damage and oversized packaging, which could be addressed through improved packaging design and machinery, customized packaging sizes, and consumer education. The packaging design must be adapted to address these key issues and simultaneously consider geographical areas, specific food products and types of packaging. Further research is needed to explore these connections more comprehensively and improve data comparability across methodologies and nomenclature.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"20 1","pages":"5 - 17"},"PeriodicalIF":1.4,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-024-01539-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}