Journal of Herbal Medicine最新文献

{"title":"Chemical analysis and bioactivity evaluation of Citrus limon leaves volatile oil from Palestine: investigating phytochemical, anti-inflammatory, antimicrobial, and cytotoxic properties","authors":"Nidal Jaradat ,&nbsp;Mohammed Hawash ,&nbsp;Murad Abualhasan ,&nbsp;Nawaf Al-Maharik ,&nbsp;Mohammad Qadi ,&nbsp;Raneen Qabaha ,&nbsp;Khariya Amarneh ,&nbsp;Aya Qabaha ,&nbsp;Sama’ Qassarwi ,&nbsp;Linda Issa ,&nbsp;Trobjon Makhkamov ,&nbsp;Nilufar Ergasheva ,&nbsp;Abdumurod Sattarov","doi":"10.1016/j.hermed.2024.100954","DOIUrl":"10.1016/j.hermed.2024.100954","url":null,"abstract":"<div><h3>Introduction</h3><div>Scientists consider repurposing discarded waste into valuable resources for the pharmaceutical, cosmetic, and food flavoring manufacturing industries a challenge to generate new revenue streams, lower production costs, and reduce waste. Therefore, the current study aimed to identify <em>Citrus limon</em> leaf volatile oil (VO) constituents from Palestine and assess its in vitro antimicrobial, anti-inflammatory, and cytotoxic properties.</div></div><div><h3>Methods</h3><div>The components of the VO were analyzed using Gas Chromatography/Mass Spectrometry (GC/MS). The antimicrobial activity of VO was assessed using a microdilution technique. The cyclooxygenases (COX-1 and COX-2) assay was utilized to assess the anti-inflammatory effect. An aqueous one solution cell proliferation (MTS) assay was employed to determine the cytotoxic effect of the VO.</div></div><div><h3>Results</h3><div>Thirty-six molecules were identified in the oil, and geranial, neral, and limonene are the most abundant molecules, comprising 31.06%, 23.98%, and 14.32%, respectively. The antimicrobial results showed that the VO has notable inhibition against <em>Proteus vulgaris,</em> Methicillin-resistant <em>Staphylococcus aureus</em>, and <em>Candida albicans</em>. The COX IC₅₀ calculations revealed that it has high potency against COX-2 IC₅₀ <!-->=<!--> <!-->9.32<!--> <!-->±<!--> <!-->0.88<!--> <!-->µg/ml. The most potent cytotoxic effect of <em>C. limon</em> VO was noticed against MCF-7, with an IC₅₀ dose of 162.90<!--> <!-->±<!--> <!-->1.85 mg/ml.</div></div><div><h3>Conclusion</h3><div>The GC-MS analyses revealed that geranial, neral, and limonene are the predominant compounds in the VO of <em>C. limon</em> leaves from Palestine. The biological test results demonstrated that the VO has potential inhibitory actions against some cancer cells, bacterial and fungal species, and COX-2 enzyme, suggesting it may be a viable choice for treating or preventing microbial infections, cancer, and inflammatory illnesses.</div></div>","PeriodicalId":56077,"journal":{"name":"Journal of Herbal Medicine","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}