Journal of Antibiotics最新文献_第8页

Actinomycetospora termitidis sp. nov., an insect-derived actinomycete isolated from termite (Odontotermes formosanus) 白蚁放线菌（Actinomycetospora termitidis sp.）

IF 3.3 4区医学

Journal of Antibiotics Pub Date : 2024-03-25 DOI: 10.1038/s41429-024-00712-8

Khomsan Supong, Nantawan Niemhom, Chanwit Suriyachadkun, Wongsakorn Phongsopitanun, Somboon Tanasupawat, Pattama Pittayakhajonwut

{"title":"Actinomycetospora termitidis sp. nov., an insect-derived actinomycete isolated from termite (Odontotermes formosanus)","authors":"Khomsan Supong, Nantawan Niemhom, Chanwit Suriyachadkun, Wongsakorn Phongsopitanun, Somboon Tanasupawat, Pattama Pittayakhajonwut","doi":"10.1038/s41429-024-00712-8","DOIUrl":"10.1038/s41429-024-00712-8","url":null,"abstract":"Strain Odt1-22T, an insect-derived actinomycete was isolated from a termite (Odontotermes formosanus) that was collected from Chanthaburi province, Thailand. Strain Odt1-22T was aerobic, Gram-stain-positive, and produced bud-like spore chain on the substrate hypha. According to chemotaxonomic analysis, strain Odt1-22T contained meso-diaminopimelic acid in peptidoglycan and the whole-cell hydrolysates contained arabinose, galactose, glucose, and ribose. The major menaquinone was MK-8(H4). The diagnostic phospholipids were diphosphatidylglycerol, hydroxyphosphatidylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analysis based on 16 S rRNA gene sequence revealed that strain Odt1-22T was identified to the genus Actinomycetospora and showed high similarity values with A. chiangmaiensis DSM 45062 T (99.24%), A. soli SF1T (99.24%) and A. corticicola 014-5 T (98.17%). The genomic size of strain Odt1-22T was 6.6 Mbp with 73.8% G + C content and 6355 coding sequences (CDSs). The genomic analysis, strain Odt1-22T and closely related species A. chiangmaiensis DSM 45062 T, A. soli SF1T and A. corticicola DSM 45772 T displayed the values of average nucleotide identity-blast (ANIb) at 83.7–84.1% and MUMmer (ANIm) at 86.6–87.0%. Moreover, the results of digital DNA-DNA hybridization values between strain Odt1-22T and related Actinomycetospora species were 45.8−50.5% that lower than the threshold value of commonly used to delineate separated species level. On the basis of phenotypic, chemotaxonomic, and genotypic data, strain Odt1-22T represented a novel species within the genus Actinomycetospora, for which the name Actinomycetospora termitidis sp. nov. is proposed. The type strain of the species is Odt1-22T (= TBRC 16192 T = NBRC 115965 T).","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":"77 5","pages":"299-305"},"PeriodicalIF":3.3,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140289625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tumescenamide C, a cyclic lipodepsipeptide from Streptomyces sp. KUSC_F05, exerts antimicrobial activity against the scab-forming actinomycete Streptomyces scabiei 来自链霉菌 KUSC_F05 的环脂二肽 Tumescenamide C 对疥疮形成放线菌 Streptomyces scabiei 具有抗菌活性。

IF 3.3 4区医学

Journal of Antibiotics Pub Date : 2024-03-25 DOI: 10.1038/s41429-024-00716-4

Kensuke Kaneko, Marika Mieda, Yulu Jiang, Nobuaki Takahashi, Hideaki Kakeya

{"title":"Tumescenamide C, a cyclic lipodepsipeptide from Streptomyces sp. KUSC_F05, exerts antimicrobial activity against the scab-forming actinomycete Streptomyces scabiei","authors":"Kensuke Kaneko, Marika Mieda, Yulu Jiang, Nobuaki Takahashi, Hideaki Kakeya","doi":"10.1038/s41429-024-00716-4","DOIUrl":"10.1038/s41429-024-00716-4","url":null,"abstract":"The antimicrobial activity of tumescenamide C against the scab-forming S. scabiei NBRC13768 was confirmed with a potent IC50 value (1.5 μg/mL). Three tumescenamide C-resistant S. scabiei strains were generated to compare their gene variants. All three resistant strains contained nonsynonymous variants in genes related to cellobiose/cellotriose transport system components; cebF1, cebF2, and cebG2, which are responsible for the production of the phytotoxin thaxtomin A. Decrease in thaxtomin A production and the virulence of the three resistant strains were revealed by the LC/MS analysis and necrosis assay, respectively. Although the nonsynonymous variants were insufficient for identifying the molecular target of tumescenamide C, the cell wall component wall teichoic acid (WTA) was observed to bind significantly to tumescenamide C. Moreover, changes in the WTA contents were detected in the tumescenamide C-resistant strains. These results imply that tumescenamide C targets the cell wall system to exert antimicrobial effects on S. scabiei.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":"77 6","pages":"353-364"},"PeriodicalIF":3.3,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140208200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Okichromanone, a new antiviral chromanone from a marine-derived Microbispora Okichromanone，一种来自海洋的小孢子菌的新型抗病毒色满酮。

IF 3.3 4区医学

Journal of Antibiotics Pub Date : 2024-03-22 DOI: 10.1038/s41429-024-00718-2

Marwa Elsbaey, Takahiro Jomori, Junichi Tanaka, Naoya Oku, Yasuhiro Igarashi

引用次数: 0

Three new phthalide derivatives from culture broth of Dentipellis fragilis and their cytotoxic activities 从脆弱双孢蘑菇培养液中提取的三种新的邻苯二甲酸酯衍生物及其细胞毒性活性。

IF 3.3 4区医学

Journal of Antibiotics Pub Date : 2024-03-22 DOI: 10.1038/s41429-024-00720-8

Dae-Won Ki, Dae-Cheol Choi, Yeong-Seon Won, Seung-Jae Lee, Young-Hee Kim, In-Kyoung Lee, Bong-Sik Yun

引用次数: 0

Uncovering the potentiality of quinazoline derivatives against Pseudomonas aeruginosa with antimicrobial synergy and SAR analysis 通过抗菌协同作用和 SAR 分析揭示喹唑啉衍生物抗铜绿假单胞菌的潜力。

IF 3.3 4区医学

Journal of Antibiotics Pub Date : 2024-03-21 DOI: 10.1038/s41429-024-00717-3

Rakshit Manhas, Arti Rathore, Ujwal Havelikar, Shavi Mahajan, Sumit G. Gandhi, Avisek Mahapa

{"title":"Uncovering the potentiality of quinazoline derivatives against Pseudomonas aeruginosa with antimicrobial synergy and SAR analysis","authors":"Rakshit Manhas, Arti Rathore, Ujwal Havelikar, Shavi Mahajan, Sumit G. Gandhi, Avisek Mahapa","doi":"10.1038/s41429-024-00717-3","DOIUrl":"10.1038/s41429-024-00717-3","url":null,"abstract":"Antimicrobial resistance has emerged as a covert global health crisis, posing a significant threat to humanity. If left unaddressed, it is poised to become the foremost cause of mortality worldwide. Among the multitude of resistant bacterial pathogens, Pseudomonas aeruginosa, a Gram-negative, facultative bacterium, has been responsible for mild to deadly infections. It is now enlisted as a global critical priority pathogen by WHO. Urgent measures are required to combat this formidable pathogen, necessitating the development of novel anti-pseudomonal drugs. To confront this pressing issue, we conducted an extensive screening of 3561 compounds from the ChemDiv library, resulting in the discovery of potent anti-pseudomonal quinazoline derivatives. Among the identified compounds, IDD-8E has emerged as a lead molecule, exhibiting exceptional efficacy against P. aeruginosa while displaying no cytotoxicity. Moreover, IDD-8E demonstrated significant pseudomonal killing, disruption of pseudomonal biofilm and other anti-bacterial properties comparable to a well-known antibiotic rifampicin. Additionally, IDD-8E’s synergy with different antibiotics further strengthens its potential as a powerful anti-pseudomonal agent. IDD-8E also exhibited significant antimicrobial efficacy against other ESKAPE pathogens. Moreover, we elucidated the Structure-Activity-Relationship (SAR) of IDD-8E targeting the essential WaaP protein in P. aeruginosa. Altogether, our findings emphasize the promise of IDD-8E as a clinical candidate for novel anti-pseudomonal drugs, offering hope in the battle against antibiotic resistance and its devastating impact on global health.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":"77 6","pages":"365-381"},"PeriodicalIF":3.3,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140186338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure of prolylrapamycin: confirmation through a revised and detailed NMR assignment study 丙烯拉霉素的结构：通过修订和详细的核磁共振赋值研究加以确认

IF 3.3 4区医学

Journal of Antibiotics Pub Date : 2024-03-19 DOI: 10.1038/s41429-024-00714-6

Annalisa Mortoni, Eugenio Castelli, Teresa Recca, Paolo Quadrelli

引用次数: 0

Correlation between the spread of IMP-producing bacteria and the promoter strength of blaIMP genes 产 IMP 细菌的传播与 blaIMP 基因启动子强度之间的相关性。

IF 3.3 4区医学

Journal of Antibiotics Pub Date : 2024-03-15 DOI: 10.1038/s41429-024-00715-5

Yuta Kikuchi, Mariko Yoshida, Asaomi Kuwae, Yukihiro Asami, Yuki Inahashi, Akio Abe

{"title":"Correlation between the spread of IMP-producing bacteria and the promoter strength of blaIMP genes","authors":"Yuta Kikuchi, Mariko Yoshida, Asaomi Kuwae, Yukihiro Asami, Yuki Inahashi, Akio Abe","doi":"10.1038/s41429-024-00715-5","DOIUrl":"10.1038/s41429-024-00715-5","url":null,"abstract":"The first report of transmissible carbapenem resistance encoded by blaIMP-1 was discovered in Pseudomonas aeruginosa GN17203 in 1988, and blaIMP-1 has since been detected in other bacteria, including Enterobacterales. Currently, many variants of blaIMPs exist, and point mutations in the blaIMP promoter have been shown to alter promoter strength. For example, the promoter (Pc) of blaIMP-1, first reported in P. aeruginosa GN17203, was a weak promoter (PcW) with low-level expression intensity. This study investigates whether point mutations in the promoter region have helped to create strong promoters under antimicrobial selection pressure. Using bioinformatic approaches, we retrieved 115 blaIMPs from 14,529 genome data of Pseudomonadota and performed multiple alignment analyses. The results of promoter analysis of the 115 retrieved blaIMPs showed that most of them used the Pc located in class 1 integrons (n = 112, 97.4%). The promoter analysis by year revealed that the blaIMP population with the strong promoter, PcS, was transient. In contrast, the PcW-TG population, which had acquired a TGn-extended −10 motif in PcW and had an intermediate promoter strength, gradually spread throughout the world. An inverse correlation between Pc promoter strength and Intl1 integrase excision efficiency has been reported previously [1]. Because of this trade-off, it is unlikely that blaIMPs with strong promoters will increase rapidly, but the possibility that promoter strength will increase with the use of other integrons cannot be ruled out. Monitoring of the blaIMP genes, including promoter analysis, is necessary for global surveillance of carbapenem-resistant bacteria.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":"77 5","pages":"315-323"},"PeriodicalIF":3.3,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41429-024-00715-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140141165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetics, tissue distribution, bioavailability and excretion of the anti-virulence drug Fluorothiazinon in rats and rabbits 抗病毒药物氟噻嗪酮在大鼠和兔子体内的药代动力学、组织分布、生物利用度和排泄。

IF 3.3 4区医学

Journal of Antibiotics Pub Date : 2024-03-15 DOI: 10.1038/s41429-024-00719-1

Mark V. Savitskii, Natalia E. Moskaleva, Alex Brito, Pavel A. Markin, Nailya A. Zigangirova, Anna V. Soloveva, Anna B. Sheremet, Natalia E. Bondareva, Nadezhda L. Lubenec, Franco Tagliaro, Vadim V. Tarasov, Kristina A. Tatzhikova, Svetlana A. Appolonova

{"title":"Pharmacokinetics, tissue distribution, bioavailability and excretion of the anti-virulence drug Fluorothiazinon in rats and rabbits","authors":"Mark V. Savitskii, Natalia E. Moskaleva, Alex Brito, Pavel A. Markin, Nailya A. Zigangirova, Anna V. Soloveva, Anna B. Sheremet, Natalia E. Bondareva, Nadezhda L. Lubenec, Franco Tagliaro, Vadim V. Tarasov, Kristina A. Tatzhikova, Svetlana A. Appolonova","doi":"10.1038/s41429-024-00719-1","DOIUrl":"10.1038/s41429-024-00719-1","url":null,"abstract":"Growing antimicrobial resistance has accelerated the development of anti-virulence drugs to suppress bacterial toxicity without affecting cell viability. Fluorothiazinon (FT), an anti-virulence, type three secretion system and flagella motility inhibitor which has shown promise to suppress drug-resistant pathogens having the potential to enhance the efficacy of commonly prescribed antibiotics when used in combination. In this study we characterized the pharmacokinetics, tissue distribution, bioavailability and excretion of FT in rats and rabbits. FT presented a dose-proportional linear increase in the blood of rats. Tissue distribution profiling confirmed that FT distributes to all organs being substantially higher than in the blood of rats. The bioavailability of FT was higher when administered with starch than with water implying FT should be ideally dosed with food. FT was primarily excreted in the feces in rats and rabbits while negligible amounts are recovered from the urine.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":"77 6","pages":"382-388"},"PeriodicalIF":3.3,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140141166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efflux pump inhibitor, phenylalanine-arginine beta-naphthylamide analog potentiates the activity of 5-O-mycaminosyltylonolide for multi-drug resistant Pseudomonas aeruginosa 外排泵抑制剂苯丙氨酸-精氨酸-β-萘甲酰胺类似物增强了 5-O-mycaminosyltylonolide 对多重耐药铜绿假单胞菌的活性。

IF 3.3 4区医学

Journal of Antibiotics Pub Date : 2024-03-11 DOI: 10.1038/s41429-024-00713-7

Aoi Kimishima, Masako Honsho, Junsei Terai, Paul Wasuwanich, Sota Honma, Hidehito Matsui, Hideaki Hanaki, Yukihiro Asami

引用次数: 0

New antimalarial iromycin analogs produced by Streptomyces sp. RBL-0292 链霉菌 RBL-0292 产生的新型抗疟霉素类似物。

IF 3.3 4区医学

Journal of Antibiotics Pub Date : 2024-03-04 DOI: 10.1038/s41429-024-00707-5

So-ichiro Kimura, Yoshihiro Watanabe, Shiori Shibasaki, Naoya Shinzato, Yuki Inahashi, Toshiaki Sunazuka, Rei Hokari, Aki Ishiyama, Masato Iwatsuki

引用次数: 0