Journal of Antibiotics最新文献

The secondary metabolites of the alga-derived fungus Aspergillus niveoglaucus КММ 4176 and their antimicrobial and antibiofilm activities.

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-02-21 DOI: 10.1038/s41429-025-00811-0

Nadezhda P Shlyk, Ekaterina A Yurchenko, Elena V Leshchenko, Ekaterina A Chingizova, Artur R Chingizov, Viktoria E Chausova, Natalya N Kirichuk, Yuliya V Khudyakova, Mikhail V Pivkin, Alexandr S Antonov, Roman S Popov, Marina P Isaeva, Anton N Yurchenko

{"title":"The secondary metabolites of the alga-derived fungus Aspergillus niveoglaucus КММ 4176 and their antimicrobial and antibiofilm activities.","authors":"Nadezhda P Shlyk, Ekaterina A Yurchenko, Elena V Leshchenko, Ekaterina A Chingizova, Artur R Chingizov, Viktoria E Chausova, Natalya N Kirichuk, Yuliya V Khudyakova, Mikhail V Pivkin, Alexandr S Antonov, Roman S Popov, Marina P Isaeva, Anton N Yurchenko","doi":"10.1038/s41429-025-00811-0","DOIUrl":"https://doi.org/10.1038/s41429-025-00811-0","url":null,"abstract":"Marine alga-derived fungal strain КММ 4176 was identified as Aspergillus niveoglaucus based on ITS region BenA, CaM and RPB2 gene sequence analysis. The anthraquinone derivatives emodin anthrone (1) and 4-hydroxyemodin anthrone (2), chromone derivative aloesone (3), and indole diketopiperazine alkaloid neoechinulin B (4) were isolated from the ethyl acetate extract of this fungus. In addition, UPLC MS data analysis of the KMM 4176 extract showed the presence of 17 echinulin-family alkaloids, as well as their biogenetic precursor cyclo(L-alanyl-L-tryptophyl) and a number of polyketide compounds. Emodin anthrone and 4-hydroxyemodin anthrone were found as inhibitors of biofilm formation by Staphylococcus aureus with half-maximal inhibitory concentrations (IC50) of 5.5 µM and 23.7 µM, respectively. Moreover, emodin anthrone (1) and 4-hydroxyemodin anthrone (2) inhibited staphylococcal sortase A activity with IC50 of 9.2 µM and 37.6 µM, respectively. Aloesone (3) also inhibited S. aureus biofilm formation but was less active. The first data on neoechinulin B (4) antibiofilm activity and sortase A inhibition were obtained. The positive effects of the isolated compounds on the growth of HaCaT keratinocytes infected with S. aureus were also observed.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143473314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blue benzoquinone from scorpion venom shows bactericidal activity against drug-resistant strains of the priority pathogen Acinetobacter baumannii.

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-02-18 DOI: 10.1038/s41429-025-00809-8

Ramses Gallegos-Monterrosa, Jimena I Cid-Uribe, Gustavo Delgado-Prudencio, Deyanira Pérez-Morales, María M Banda, Alexis Téllez-Galván, Edson N Carcamo-Noriega, Ulises Garza-Ramos, Richard N Zare, Lourival D Possani, Víctor H Bustamante

{"title":"Blue benzoquinone from scorpion venom shows bactericidal activity against drug-resistant strains of the priority pathogen Acinetobacter baumannii.","authors":"Ramses Gallegos-Monterrosa, Jimena I Cid-Uribe, Gustavo Delgado-Prudencio, Deyanira Pérez-Morales, María M Banda, Alexis Téllez-Galván, Edson N Carcamo-Noriega, Ulises Garza-Ramos, Richard N Zare, Lourival D Possani, Víctor H Bustamante","doi":"10.1038/s41429-025-00809-8","DOIUrl":"https://doi.org/10.1038/s41429-025-00809-8","url":null,"abstract":"Antibiotic-resistant bacteria pose a significant global health threat, particularly pathogens resistant to last-resort antibiotics, such as those listed as priority pathogens by the World Health Organization. Addressing this challenge requires the development of novel antimicrobial agents. Previously, we identified a blue 1,4-benzoquinone isolated from the venom of the Mexican scorpion Diplocentrus melici as a potent antimicrobial compound effective against Staphylococcus aureus and Mycobacterium tuberculosis. Moreover, we devised a cost-effective synthetic route for its production. In this study, we demonstrate that the blue benzoquinone exhibits antibacterial activity against additional pathogens, including the priority pathogen Acinetobacter baumannii. Notably, the compound effectively killed clinical strains of A. baumannii resistant to multiple antibiotics, including carbapenem and colistin. Furthermore, A. baumannii did not develop resistance to the benzoquinone even after multiple growth cycles under sub-inhibitory concentrations, unlike the tested antibiotics. These findings underscore the potential of this blue benzoquinone as a lead compound for the development of a new class of antibiotics targeting multidrug-resistant bacteria.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143451049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streptomyces tabacisoli sp. nov, isolated from the rhizosphere soil of Nicotiana tabacum.

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-02-17 DOI: 10.1038/s41429-024-00802-7

Kaiqin Li, Hao Zhang, Ying Qian, Ping Mo, Aihua Deng, Jian Gao, Wei Liang

{"title":"Streptomyces tabacisoli sp. nov, isolated from the rhizosphere soil of Nicotiana tabacum.","authors":"Kaiqin Li, Hao Zhang, Ying Qian, Ping Mo, Aihua Deng, Jian Gao, Wei Liang","doi":"10.1038/s41429-024-00802-7","DOIUrl":"https://doi.org/10.1038/s41429-024-00802-7","url":null,"abstract":"Strain HUAS MG91T, a novel actinobacterium, was isolated from the rhizosphere soil of Nicotiana tabacum collected from Changde City, China. This strain could form short, rod-shaped spores with a smooth surface and Rectiflexibles spore chains on Gause's synthetic No.1 medium. Comparison of 16S rRNA gene sequences showed that strain HUAS MG91T exhibited the highest similarity to Streptomyces kunmingensis NBRC 14463 T (98.95%). Phylogenetic analysis based on 16S rRNA gene and genome sequences indicated that strain HUAS MG91T was closely related to S. kunmingensis NBRC 14463 T. However, the ANIm and dDDH values between them were 87.25% and 28.60%, respectively, lower than the 96.7% ANIm threshold and 70.0% cutoff point used as guideline values for Streptomyces species delineation. On the basis of phenotypic and genotypic differentiation from tested strains, strain HUAS MG91T is proposed to represent a novel species of the genus Streptomyces, named Streptomyces tabacisoli sp. nov. The type strain is HUAS MG91T (=MCCC 1K09288T = JCM 37027 T).","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143442896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of tirandamycins with selective activity against Enterococci in the silkworm infection model.

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-02-14 DOI: 10.1038/s41429-024-00805-4

Akiho Yagi, Taku Sato, Chihiro Kano, Taeko Igari, Natsuki Oshima, Satoshi Ohte, Taichi Ohshiro, Ryuji Uchida

{"title":"Evaluation of tirandamycins with selective activity against Enterococci in the silkworm infection model.","authors":"Akiho Yagi, Taku Sato, Chihiro Kano, Taeko Igari, Natsuki Oshima, Satoshi Ohte, Taichi Ohshiro, Ryuji Uchida","doi":"10.1038/s41429-024-00805-4","DOIUrl":"https://doi.org/10.1038/s41429-024-00805-4","url":null,"abstract":"In the course of screening for anti-enterococcal antibiotics from microbial resources, a new tirandamycin congener (1), together with four known tirandamycins (2 to 5), were isolated from Streptomyces tirandamycinicus TMPU-20A040. The structures of these tirandamycins were elucidated using NMR and MS analyses; 1 was identified as 12-carboxy tirandamycin A and 2 to 5 as known tirandamycins A (2), B (3), E (4), and J (5). Compounds 1 to 3 exhibited selective anti-Enterococci activity, including vancomycin-resistant strains, with MIC in the range of 1.0 to 64 µg ml-1 in the microdilution method. 2 and 3 exerted weak therapeutic effects in the in vivo-mimic silkworm Enterococcus faecalis infection model with ED50 values of 150 and 75 µg larva-1 g-1, respectively, indicating that the in vivo activities of 2 and 3 were lower than their in vitro activities. Further investigations into the causes of the decreased in vivo activities of 2 and 3 suggested the low plasma protein binding ratio of these compounds, but revealed short half-lives of 6.3 and 16 min, respectively, in the silkworm hemolymph.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143426825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KRN7000 analogues as biofilm disrupting agents against Streptococcus pyogenes and Proteus mirabilis.

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-02-14 DOI: 10.1038/s41429-025-00810-1

K A Krishnakumar, R Remya Babu, Shiburaj Sugathan, Ravi S Lankalapalli

引用次数: 0

In vitro assessment of the synergistic effects of cefotaxime, colistin, and fosfomycin combinations against foodborne resistant Escherichia coli and Salmonella isolates.

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-02-05 DOI: 10.1038/s41429-025-00808-9

Hazim O Khalifa, Temesgen Mohammed, Mohamed-Yousif Ibrahim Mohamed, Hamada Hashem, Ihab Habib

{"title":"In vitro assessment of the synergistic effects of cefotaxime, colistin, and fosfomycin combinations against foodborne resistant Escherichia coli and Salmonella isolates.","authors":"Hazim O Khalifa, Temesgen Mohammed, Mohamed-Yousif Ibrahim Mohamed, Hamada Hashem, Ihab Habib","doi":"10.1038/s41429-025-00808-9","DOIUrl":"https://doi.org/10.1038/s41429-025-00808-9","url":null,"abstract":"The emergence of multidrug-resistant pathogens, particularly β-lactam, colistin, and fosfomycin-resistant Escherichia coli and Salmonella, is a significant public health concern. This study evaluated the in vitro synergistic effects of antimicrobial combinations against these resistant isolates. Ten isolates that originated from retail chicken meat, including five E. coli and five Salmonella isolates, were tested against cefotaxime (CTA), fosfomycin (FOS), and colistin (COL), both individually and in combinations. Antimicrobial susceptibility was assessed using the broth microdilution method, and synergistic interactions were evaluated using checkerboard and time-killing assays. All isolates were multidrug-resistant (MDR) and were resistant to CTA, COL, and FOS. The checkerboard assay showed varying levels of synergy: two out of five E. coli isolates exhibited synergy with FOS-COL, while one E. coli isolates out of four isolates showed synergy with CTA-COL. No E. coli isolates showed synergy with FOS-CTA. For Salmonella, two out of five isolates exhibited synergy with both FOS-CTA and FOS-COL, while three out of four isolates showed synergy with CTA-COL. The time-killing assay confirmed these results, with the FOS-COL combinations showing synergy against both E. coli and Salmonella strains. Notably, the FOS-COL combination demonstrated bactericidal effects against E. coli, and all three combinations were bactericidal against Salmonella. The study highlights the potential of antimicrobial combinations, particularly FOS-COL, in combating MDR E. coli and Salmonella. These findings support the use of combination therapy as a promising strategy to in effectively treating multi-drug-resistant foodborne infections, ensuring better medical outcomes and enhanced food safety, warranting further investigation into their mechanisms and clinical applications.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PPtase-activated production of borrelidin from extremophilic actinobacteria against multidrug-resistant cotton pathogen Verticillium dahliae.

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-02-05 DOI: 10.1038/s41429-025-00807-w

Fei Zhang, Xingzhi Jiao, Yuzhu Wu, Runze Sun, Chuanxing Wan, Linquan Bai, Xiaoxia Luo, Lei Li

{"title":"PPtase-activated production of borrelidin from extremophilic actinobacteria against multidrug-resistant cotton pathogen Verticillium dahliae.","authors":"Fei Zhang, Xingzhi Jiao, Yuzhu Wu, Runze Sun, Chuanxing Wan, Linquan Bai, Xiaoxia Luo, Lei Li","doi":"10.1038/s41429-025-00807-w","DOIUrl":"https://doi.org/10.1038/s41429-025-00807-w","url":null,"abstract":"As the most devastating disease in cotton crops, Verticillium wilt caused by Verticillium dahliae led to fiber quality reduction and extensive yield loss. In recent years, Verticillium wilt has been increasingly serious in Xinjiang, China, the world's largest cotton production area. In this study, 52 actinobacterial strains were selected for the discovery of NPs with anti-V. dahliae activity, from our strain collection isolated from three types of extreme habitats (high salinity, high temperature or plant endophytes) in Xinjiang. In the culture broth extracts of phosphopantetheinyl transferase (PPtase)-activated actinobacterial strains, four showed good anti-V. dahliae activities. By isolation, purification and spectral analysis of the antifungal metabolite, its structure was elucidated as borrelidin from Streptomyces rochei TRM46813. This is the first report that borrelidin shows anti-V. dahliae activity. Notably, borrelidin could effectively inhibit different commercial fungicides-resistant V. dahliae pathogens with MICs of 0.125 to 2 μg/mL. The growth-inhibitory effect was antagonized by L-threonine in a dose-dependent manner, suggesting that threonyl-tRNA synthetase (ThrRS) may be the target of borrelidin in V. dahliae. Interestingly, although borrelidin was previously reported to bind to ThrRS and thus inhibit protein translation in Gram-positive pathogens, our mode-of-action analysis indicates that borrelidin led to the accumulation of cell wall precursor, which may be due to the inhibited effects on protein translation. Our findings suggest that borrelidin is a promising candidate for the development of novel antifungal agents to overcome the growing problem of Verticillium wilt.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Substance Index for Volume 77

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-01-27 DOI: 10.1038/s41429-024-00786-4

引用次数: 0

Author Index for Volume 77

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-01-27 DOI: 10.1038/s41429-024-00787-3

引用次数: 0

Investigating the validity of mCIM and sCIM phenotypic methods in screening Pseudomonas aeruginosa isolates producing IMP, VIM, and NDM metallo-beta-lactamases isolated from burn wounds. 探讨mCIM和sCIM表型方法在筛选从烧伤创面分离的产生IMP、VIM和NDM金属内酰胺酶的铜绿假单胞菌分离株中的有效性。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-01-21 DOI: 10.1038/s41429-025-00806-x

Hossein Hatami, Shiva Motamedi, Ghazaleh Talebi, Mojdeh Hakemi-Vala

{"title":"Investigating the validity of mCIM and sCIM phenotypic methods in screening Pseudomonas aeruginosa isolates producing IMP, VIM, and NDM metallo-beta-lactamases isolated from burn wounds.","authors":"Hossein Hatami, Shiva Motamedi, Ghazaleh Talebi, Mojdeh Hakemi-Vala","doi":"10.1038/s41429-025-00806-x","DOIUrl":"https://doi.org/10.1038/s41429-025-00806-x","url":null,"abstract":"Metallo-beta-lactamase-producing Pseudomonas aeruginosa (P. aeruginosa) is a major pathogen in burn wounds, often exhibiting high levels of antibiotic resistance, which complicates treatment strategies. This study deals with the validity of the modified Carbapenem Inactivation Method (mCIM) and the simplified Carbapenem Inactivation Method (sCIM) phenotypic tests for screening metallo-beta-lactamase (MBL) production by P. aeruginosa isolates from a referral burn center in Iran. Forty isolates were obtained between January and June 2021 and identified using conventional biochemical methods. Antimicrobial susceptibility testing was conducted following Clinical and Laboratory Standards Institute (CLSI) 2021 guidelines. mCIM based on CLSI 2023 guidelines was used to detect carbapenemase production. sCIM was also used based on previously developed protocols. PCR was performed to detect blaIMP, blaVIM, and blaNDM genes. The results were analyzed using SPSS and MedCalc. We observed a 90% resistance rate to imipenem and high resistance to other antibiotics, with multidrug-resistant (MDR) strains constituting 95% of the isolates. The mCIM test demonstrated high sensitivity (87.50%) and high negative predictive value (89.47%) and moderate specificity (70.83%) and moderate positive predictive value (66.67%) for detecting MBLs. In contrast, the sCIM test was unreliable, indicating a need for more standardized testing protocols. This study underscores the importance of accurate and timely detection of carbapenemase production to guide effective treatment.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0