Journal of Antibiotics最新文献

Streptomyces secundicynarae sp. nov., a novel actinomycete isolated from the leaves of Cynara scolymus. 次级链霉菌（Streptomyces secondicynarae sp. nov.），一种从猪头菇（Cynara scolymus）叶片中分离的新型放线菌。

IF 2.7 4区医学

Journal of Antibiotics Pub Date : 2025-07-29 DOI: 10.1038/s41429-025-00853-4

Yu Song, Ting Tang, Ping Mo, Zhilong Wu, Fengying Liu, Aihua Deng

{"title":"Streptomyces secundicynarae sp. nov., a novel actinomycete isolated from the leaves of Cynara scolymus.","authors":"Yu Song, Ting Tang, Ping Mo, Zhilong Wu, Fengying Liu, Aihua Deng","doi":"10.1038/s41429-025-00853-4","DOIUrl":"10.1038/s41429-025-00853-4","url":null,"abstract":"A novel actinobacterium strain, designated HUAS ZL42T, was isolated from the leaves of Cynara scolymus. Its taxonomic position was characterized using a polyphasic method. Strain HUAS ZL42T produced spiral spore chains consisting of rod-shaped or ovoid spores with smooth surfaces on Gause's synthetic No.1 medium. The cell wall of strain HUAS ZL42T contained LL-diaminopimelic acid as a diagnostic amino acid. Whole-cell hydrolysates contained galactose and mannose. The predominant cellular fatty acids (>10.0%) of strain HUAS ZL42T were iso-C14:0, anteiso-C15:0 and iso-C16:0. The menaquinones were MK-9 (H6), MK-9 (H4) and MK-9 (H8). The 16S rRNA gene sequence analysis showed that strain HUAS ZL42T exhibited the highest similarities to Streptomyces cyslabdanicus K04-0144T (98.6%), Streptomyces cinnabarigriseus JS360T (98.6%), Streptomyces cyaneus CGMCC 4.1671T (98.4%) and Streptomyces corchorusii DSM 40340T (98.4%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HUAS ZL42T formed an independent subclade. Phylogenetic analysis based on five housekeeping gene sequences demonstrated that strain HUAS ZL42T was most closely related to S. cyaneus CGMCC 4.1671T. However, the ANIm and dDDH between strain HUAS ZL42T and S. cyaneus CGMCC 4.1671T were 87.17% and 28.90%, respectively, far less than 96.7% and 70% cut-off points recommended for delineating Streptomyces species, suggesting that strain HUAS ZL42T was a novel Streptomyces species. On the basis of phenotypic and genotypic differentiation from tested strains, it is proposed that strain HUAS ZL42T represents a novel species of the genus Streptomyces, named Streptomyces secundicynarae sp. nov. The type strain is HUAS ZL42T (=MCCC 1K09444T = JCM 37325T).","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144746001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emericellopsic acid, a helvolic acid derivative with a 6/5/7/5 tetracyclic skeleton from sponge-derived Emericellopsis maritima. 海苔绿藻酸：一种从海苔绿藻中提取的具有6/5/7/5四环骨架的helvolic衍生物。

IF 2.7 4区医学

Journal of Antibiotics Pub Date : 2025-07-29 DOI: 10.1038/s41429-025-00852-5

Shasha Li, Xiaomeng Hao, Yan Li, Maoluo Gan

引用次数: 0

New benzoyl sesquiterpenoid, aoganolide, produced by Talaromyces sp. KTF-0021 strain (laeA-introduced mutant of FKI-5759 strain). Talaromyces sp. KTF-0021菌株（FKI-5759菌株laea引种突变体）产新苯甲酰倍半萜类。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-07-25 DOI: 10.1038/s41429-025-00851-6

Karin Hamada, Yoshihiro Watanabe, Yuta Kikuchi, Hayama Tsutsumi, Haruki Azami, Hiroki Kojima, Sho Kato, Akihiro Sugawara, Yuki Inahashi, Kenichi Nonaka, Rei Hokari, Aki Ishiyama, Yasuko Araki, Kona Yamashita, Tadashi Takahashi, Kotaro Ito, Yukihiro Asami, Masato Iwatsuki

{"title":"New benzoyl sesquiterpenoid, aoganolide, produced by Talaromyces sp. KTF-0021 strain (laeA-introduced mutant of FKI-5759 strain).","authors":"Karin Hamada, Yoshihiro Watanabe, Yuta Kikuchi, Hayama Tsutsumi, Haruki Azami, Hiroki Kojima, Sho Kato, Akihiro Sugawara, Yuki Inahashi, Kenichi Nonaka, Rei Hokari, Aki Ishiyama, Yasuko Araki, Kona Yamashita, Tadashi Takahashi, Kotaro Ito, Yukihiro Asami, Masato Iwatsuki","doi":"10.1038/s41429-025-00851-6","DOIUrl":"https://doi.org/10.1038/s41429-025-00851-6","url":null,"abstract":"To discover novel natural compounds by awakening unexplored or silent BGCs in fungi, our research group has explored the cultured broths of the laeA-introduced mutant fungal strains. In this study, we report the isolation of a new benzoyl sesquiterpenoid, aoganolide (4), as well as three known compounds, decarboxyaltenusin (1), altenusin (2), and penipyranicin B (3), from the cultured broth of Talaromyces sp. KTF-0021, which was a laeA-introduced mutant of FKI-5759 strain. The planar structure of 4 was elucidated by spectroscopic analysis and the absolute configuration of 4 was determined by the calculated ECD spectral method. Among them, 4 showed antimalarial activity with IC50 values of 4.37 and 6.46 µg ml-1 against Plasmodium falciparum FCR3 and K1 strains, respectively. The laeA-introduced mutant strain produced 2, 3, and 4 with higher productivity (47, 623, and 38 mg l-1) than the wild-type strain (1.4, 0.8, and 7.7 mg l-1), respectively, suggesting this method is useful to expand the chemical diversity of natural products.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144719070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioinformatics-driven discovery of skin microbiota bacteriocins as potential antibiotics and probiotics. 生物信息学驱动的皮肤微生物群细菌素作为潜在的抗生素和益生菌的发现。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-07-24 DOI: 10.1038/s41429-025-00847-2

Anum Munir, Alan Janbey, Bilawal Sajjad, Kowsalya V Perumal, Hajra Qayyum, Marriam Bakhtiar

{"title":"Bioinformatics-driven discovery of skin microbiota bacteriocins as potential antibiotics and probiotics.","authors":"Anum Munir, Alan Janbey, Bilawal Sajjad, Kowsalya V Perumal, Hajra Qayyum, Marriam Bakhtiar","doi":"10.1038/s41429-025-00847-2","DOIUrl":"https://doi.org/10.1038/s41429-025-00847-2","url":null,"abstract":"The human skin microbiota, comprising a diverse range of microorganisms, including bacteria, viruses, and fungi, plays an important role in maintaining skin health and protecting against pathogenic invasions. Among these microorganisms, certain bacteria produce bacteriocins, which are ribosomal peptides with potent antimicrobial properties. This study presents a novel computational approach to identify and predict bacteriocins from microbial genomes comprising sebaceous region of the skin, aiming to explore their therapeutic potential. Through genome analysis using advanced bioinformatics tools, we identified potential genes, operons, open reading frames (ORFs), and promoter regions linked to bacteriocin production. The BAGEL4 platform was employed to detect structural bacteriocin genes, while modelling bacterial growth and bacteriocin expression under various environmental conditions was conducted using MATLAB's SimBiology application. The results revealed the optimal conditions for bacteriocin production and highlighted promising candidates for further experimental validation. These findings underscore the significance of skin microbiota as a source of novel bacteriocins, offering potential alternatives to traditional antibiotics amidst rising antimicrobial resistance.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144700409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New amino-naphthoic acid derivatives produced by biological transformation using the deep-sea-derived bacterium Serinicoccus marinus KDM482. 深海来源的海洋丝氨酸球菌KDM482生物转化制备新的氨基萘衍生物。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-07-23 DOI: 10.1038/s41429-025-00845-4

Mai Sakaguchi, Katsuki Kikuchi, Ryota Okamura, Akito Taniguchi, Kenichiro Nagai, Reiko Seki, Masashi Ando, Teruyoshi Tanaka, Takashi Fukuda

引用次数: 0

In vivo efficacy of atorvastatin in the treatment of Tinea pedis: stepping forward into drug repositioning. 阿托伐他汀治疗足癣的体内疗效：迈向药物重新定位。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-07-16 DOI: 10.1038/s41429-025-00848-1

Ari Soares de Oliveira Neto, Maria Eliza Samuel Amorim, Rodrigo Luiz Fabri, Rene Oliveira do Couto, Marcelo Gonzaga de Freitas Araújo

引用次数: 0

The effect of exogenous glutathione on meropenem susceptibility in Klebsiella pneumoniae-carbapenemases (KPC)-producing bacteria. 外源性谷胱甘肽对肺炎克雷伯菌碳青霉烯酶（KPC）产生菌美罗培南敏感性的影响。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-07-14 DOI: 10.1038/s41429-025-00850-7

Dong H Kwon, Mital Vasoya, Danya Sankaranarayanan

{"title":"The effect of exogenous glutathione on meropenem susceptibility in Klebsiella pneumoniae-carbapenemases (KPC)-producing bacteria.","authors":"Dong H Kwon, Mital Vasoya, Danya Sankaranarayanan","doi":"10.1038/s41429-025-00850-7","DOIUrl":"https://doi.org/10.1038/s41429-025-00850-7","url":null,"abstract":"Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae and Pseudomonas aeruginosa, associated with systemic and hospital-acquired infections, have spread globally and pose a significant public health concern. Glutathione is a multifunctional thiol-antioxidant compound synthesized in most Gram-negative bacteria and crucial in maintaining intracellular redox homeostasis. Exogenous glutathione exhibits antibiotic properties and has differential effects on conventional antibiotics. Therefore, its effect on specific antibiotics needs to be clarified in bacterial species. In this study, we investigated the antibacterial activity of glutathione and its effect on meropenem susceptibility in KPC-producing bacteria. Two major KPC-encoding genes cloned from two different clinical KPC-producing K. pneumoniae were introduced into E. coli and P. aeruginosa. Then, the KPC-producing K. pneumoniae, E. coli, and P. aeruginosa were used for minimum inhibitory concentration (MIC), population analysis, checkerboard, and time-killing assays. The results showed that glutathione exhibited antibacterial activity at >10 mM in K. pneumoniae, E. coli, and P. aeruginosa. MIC levels of meropenem combined with 10 mM of glutathione were synergistically decreased by 8- to ≥ 256-fold in KPC-producing bacteria. Furthermore, this combination killed 100% of the KPC-producing bacteria at 2 to 4 μg mL-1 of meropenem. These findings suggest that exogenous glutathione may be applicable in fighting infections caused by KPC-producing bacteria.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Halobacin, a herbicidal acyloin derivative from a marine bacterium of the genus Halobacillus. 盐杆菌素，从盐杆菌属的海洋细菌中提取的一种除草剂半胱氨酸。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-07-10 DOI: 10.1038/s41429-025-00842-7

Md Mehebub Al Raji, Desy Wulan Triningsih, Agus Trianto, Yasuhiro Igarashi

引用次数: 0

Altered gene expression of cold shock proteins under antibiotic exposure. 抗生素暴露下冷休克蛋白基因表达的改变。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-07-09 DOI: 10.1038/s41429-025-00849-0

Evieann Cardoza, Darsh Vira, Advait Rao, Harinder Singh

{"title":"Altered gene expression of cold shock proteins under antibiotic exposure.","authors":"Evieann Cardoza, Darsh Vira, Advait Rao, Harinder Singh","doi":"10.1038/s41429-025-00849-0","DOIUrl":"https://doi.org/10.1038/s41429-025-00849-0","url":null,"abstract":"Stressors like translational inhibitors stall protein synthesis and produce a response specific to temperature extremes. Yet, little is known about the expression of temperature-related proteins, particularly the cold shock proteins (Csps), under antibiotic stress. Here, we demonstrate the expression pattern of all nine Csps of Escherichia coli to a sub-lethal concentration of chloramphenicol, tetracycline, gentamicin, kanamycin, and ampicillin. The five antibiotics represent different classes that target different areas of the translational apparatus and the cell wall. To investigate whether all nine csps are expressed in response to antibiotics, we measured the survival of E. coli across the antibiotics and further analyzed expression by qPCR. We find that the expression pattern of csps varies between the Csp groups, and within a Csp group, certain members are more prominently expressed than the rest. The C-group antibiotics, which include chloramphenicol and tetracycline, upregulated the expression of cold-inducible and uncharacterized Csp groups. The H-group antibiotic, kanamycin, along with the uncharacterized antibiotics gentamicin and ampicillin, induced csps as well as heat shock proteins (hsps). To the best of our knowledge, this study is the first to demonstrate the expression pattern of all nine csps in response to antibiotics. Moreover, our study has implications for understanding the triggers of Csps and, in a broader context, their role in stress tolerance, virulence, and pathogenesis.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Griseolutein T from Streptomyces seoulensis, newly identified via combined-culture with Tsukamurella pulmonis, as an efficacious therapeutic agent against multidrug-resistant bacteria. 首尔链霉菌灰质蛋白T与肺恙螨联合培养新发现的抗多药耐药菌的有效药物。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-07-09 DOI: 10.1038/s41429-025-00846-3

Sung-Jin Kawai, Shumpei Asamizu, Hiroaki Suzuki, Hiroyasu Onaka, Yoshichika Arakawa, Kouji Kimura, Makoto Ojika

{"title":"Griseolutein T from Streptomyces seoulensis, newly identified via combined-culture with Tsukamurella pulmonis, as an efficacious therapeutic agent against multidrug-resistant bacteria.","authors":"Sung-Jin Kawai, Shumpei Asamizu, Hiroaki Suzuki, Hiroyasu Onaka, Yoshichika Arakawa, Kouji Kimura, Makoto Ojika","doi":"10.1038/s41429-025-00846-3","DOIUrl":"https://doi.org/10.1038/s41429-025-00846-3","url":null,"abstract":"Bacterial interactions can affect the production of secondary metabolites and, therefore, provide a promising approach to exploring new microbial compounds. In this study, we screened actinomycetes isolated from Hegura Island, Ishikawa Prefecture, Japan, to discover new antibiotics through combined-culture with Tsukamurella pulmonis TP-B0596. Three new phenazine-class antibiotics, griseoluteins T (1), C (2), and D (3), along with two known related metabolites, griseoluteic acid (4) and griseolutein A (5), were detected in both mono- and combined-cultures of Streptomyces seoulensis HEK131 with T. pulmonis at different production levels. Detailed spectroscopic analysis revealed that 1 contained a dihydrophenazine core, and was converted to 5 by accepting oxidation spontaneously. 1, containing a dihydrophenazine group, was relatively unstable under oxidative conditions, and the addition of ascorbate was required during the isolation of the compound. 2 and 3 were found to be cysteine-adducts analogous to 4, and their productivity was increased in the combined-culture. We further assessed the antibacterial activities of 1 against clinically significant Gram-positive pathogenic bacteria, including 30 methicillin-resistant Staphylococcus aureus (MRSA), 27 vancomycin-resistant Enterococci (VRE), and 17 Clostridioides difficile. Notably, 1 was found to possess higher antibacterial activity against these microorganisms than several clinically important antibiotics, while displaying lower cytotoxicity against HeLa-S3 cells.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0