次级链霉菌（Streptomyces secondicynarae sp. nov.），一种从猪头菇（Cynara scolymus）叶片中分离的新型放线菌。

IF 2.7 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Antibiotics Pub Date : 2025-07-29 DOI:10.1038/s41429-025-00853-4

Yu Song, Ting Tang, Ping Mo, Zhilong Wu, Fengying Liu, Aihua Deng

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引用次数: 0

摘要

从猪头草（Cynara scolymus）叶片中分离到一株放线菌，命名为HUAS ZL42T。用多相法对其分类位置进行了鉴定。菌株HUAS ZL42T在Gause合成1号培养基上产生表面光滑的杆状或卵球形螺旋孢子链。菌株HUAS ZL42T细胞壁含有l -二氨基亚苯酸作为诊断氨基酸。全细胞水解物含有半乳糖和甘露糖。菌株HUAS ZL42T的主要细胞脂肪酸为iso-C14:0、anteiso-C15:0和iso-C16:0（>10.0%）。甲基萘醌类分别为MK-9 （H6）、MK-9 （H4）和MK-9 （H8）。16S rRNA基因序列分析显示，菌株HUAS ZL42T与cylabdanstreptomyces K04-0144T（98.6%）、朱红链霉菌JS360T（98.6%）、蓝链霉菌CGMCC 4.1671T（98.4%）和corchorusii Streptomyces DSM 40340T（98.4%）的相似性最高。基于16S rRNA基因序列的系统发育分析表明，菌株HUAS ZL42T形成一个独立的亚支系。基于5个管家基因序列的系统发育分析表明，菌株HUAS ZL42T与S. cyaneus CGMCC 4.1671T亲缘关系最为密切。菌株HUAS ZL42T与S. cyaneus CGMCC 4.1671T之间的ANIm和dDDH分别为87.17%和28.90%，远低于推荐的链霉菌种划分截断点96.7%和70%，提示菌株HUAS ZL42T为链霉菌新种。根据与被试菌株的表型和基因型分化，提出菌株HUAS ZL42T代表链霉菌属的一个新种，命名为Streptomyces secundicynarae sp. nov.，型菌株为HUAS ZL42T （=MCCC 1K09444T = JCM 37325T）。

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Streptomyces secundicynarae sp. nov., a novel actinomycete isolated from the leaves of Cynara scolymus.

A novel actinobacterium strain, designated HUAS ZL42^T, was isolated from the leaves of Cynara scolymus. Its taxonomic position was characterized using a polyphasic method. Strain HUAS ZL42^T produced spiral spore chains consisting of rod-shaped or ovoid spores with smooth surfaces on Gause's synthetic No.1 medium. The cell wall of strain HUAS ZL42^T contained LL-diaminopimelic acid as a diagnostic amino acid. Whole-cell hydrolysates contained galactose and mannose. The predominant cellular fatty acids (>10.0%) of strain HUAS ZL42^T were iso-C_14:0, anteiso-C_15:0 and iso-C_16:0. The menaquinones were MK-9 (H₆), MK-9 (H₄) and MK-9 (H₈). The 16S rRNA gene sequence analysis showed that strain HUAS ZL42^T exhibited the highest similarities to Streptomyces cyslabdanicus K04-0144^T (98.6%), Streptomyces cinnabarigriseus JS360^T (98.6%), Streptomyces cyaneus CGMCC 4.1671^T (98.4%) and Streptomyces corchorusii DSM 40340^T (98.4%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HUAS ZL42^T formed an independent subclade. Phylogenetic analysis based on five housekeeping gene sequences demonstrated that strain HUAS ZL42^T was most closely related to S. cyaneus CGMCC 4.1671^T. However, the ANIm and dDDH between strain HUAS ZL42^T and S. cyaneus CGMCC 4.1671^T were 87.17% and 28.90%, respectively, far less than 96.7% and 70% cut-off points recommended for delineating Streptomyces species, suggesting that strain HUAS ZL42^T was a novel Streptomyces species. On the basis of phenotypic and genotypic differentiation from tested strains, it is proposed that strain HUAS ZL42^T represents a novel species of the genus Streptomyces, named Streptomyces secundicynarae sp. nov. The type strain is HUAS ZL42^T (=MCCC 1K09444^T = JCM 37325^T).

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来源期刊

Journal of Antibiotics 医学-免疫学

CiteScore

6.60

自引率

3.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Antibiotics seeks to promote research on antibiotics and related types of biologically active substances and publishes Articles, Review Articles, Brief Communication, Correspondence and other specially commissioned reports. The Journal of Antibiotics accepts papers on biochemical, chemical, microbiological and pharmacological studies. However, studies regarding human therapy do not fall under the journal’s scope. Contributions regarding recently discovered antibiotics and biologically active microbial products are particularly encouraged. Topics of particular interest within the journal''s scope include, but are not limited to, those listed below: Discovery of new antibiotics and related types of biologically active substances Production, isolation, characterization, structural elucidation, chemical synthesis and derivatization, biological activities, mechanisms of action, and structure-activity relationships of antibiotics and related types of biologically active substances Biosynthesis, bioconversion, taxonomy and genetic studies on producing microorganisms, as well as improvement of production of antibiotics and related types of biologically active substances Novel physical, chemical, biochemical, microbiological or pharmacological methods for detection, assay, determination, structural elucidation and evaluation of antibiotics and related types of biologically active substances Newly found properties, mechanisms of action and resistance-development of antibiotics and related types of biologically active substances.