Journal of Antibiotics最新文献_第2页

Streptomyces maremycinicus sp. nov. and its secondary metabolite-biosynthetic gene clusters. 马雷霉素链霉菌及其次生代谢-生物合成基因簇。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-07-09 DOI: 10.1038/s41429-025-00844-5

Hisayuki Komaki, Yasuhiro Igarashi, Tomohiko Tamura

{"title":"Streptomyces maremycinicus sp. nov. and its secondary metabolite-biosynthetic gene clusters.","authors":"Hisayuki Komaki, Yasuhiro Igarashi, Tomohiko Tamura","doi":"10.1038/s41429-025-00844-5","DOIUrl":"https://doi.org/10.1038/s41429-025-00844-5","url":null,"abstract":"Streptomyces strain TP-A0890T, isolated from a soil sample, is a producer of FR-900452 and A-74863a. The taxonomic status was clarified by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Streptomyces coriariae, with similarity of 99.7%. Strain TP-A0890T comprised LL-diaminopimelic acid, glutamic acid, glycine and alanine in its peptidoglycan. The predominant menaquinones were MK-9(H8) and MK-9(H6), and major fatty acids were anteiso-C17:0, anteiso-C15:0, iso-C16:0 and iso-C17:0. The chemotaxonomic features matched those described for the genus Streptomyces. The genome size and G + C content were 8.72 Mb and 71.5%, respectively. The results of digital DNA-DNA hybridization along with differences in phenotypic characteristics between the strains suggested strain TP-A0890T to assign to a novel species, for which Streptomyces maremycinicus sp. nov. is proposed; the type strain is TP-A0890T ( = NBRC 110468T). We also show that Streptomyces strain B9173, a producer of FR-900452 and maremycins that was isolated from coastal sediment in Chile, belonged to S. maremycinicus. Twenty-two to 23 secondary metabolite-biosynthetic gene clusters (smBGCs) were present in the genomes of S. maremycinicus strains. Seventeen of them were conserved in the genome of S. coriariae CMB-FBT but the others were not.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144602284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The antirheumatic drug sulfasalazine ameliorates acute renal failure (ARF) induced by polymyxin B. 抗风湿药物磺胺氮嗪可改善多粘菌素B诱导的急性肾功能衰竭。

IF 2.7 4区医学

Journal of Antibiotics Pub Date : 2025-07-01 Epub Date: 2025-06-02 DOI: 10.1038/s41429-025-00835-6

Kohei Otani, Tomohiro Kagi, Takuya Noguchi, Sara Suzuki, Yusuke Hirata, Atsushi Matsuzawa

引用次数: 0

Micrococcins from Peribacillus sp. KDM594; efficacy against vancomycin-resistant enterococci and drug metabolism in a silkworm model. 从周芽孢杆菌sp. KDM594提取微球菌素家蚕模型抗万古霉素耐药肠球菌疗效及药物代谢。

IF 2.7 4区医学

Journal of Antibiotics Pub Date : 2025-07-01 Epub Date: 2025-06-11 DOI: 10.1038/s41429-025-00838-3

Akiho Yagi, Mayu Sato, Katsuki Kikuchi, Akito Taniguchi, Takashi Fukuda, Ryuji Uchida

{"title":"Micrococcins from Peribacillus sp. KDM594; efficacy against vancomycin-resistant enterococci and drug metabolism in a silkworm model.","authors":"Akiho Yagi, Mayu Sato, Katsuki Kikuchi, Akito Taniguchi, Takashi Fukuda, Ryuji Uchida","doi":"10.1038/s41429-025-00838-3","DOIUrl":"10.1038/s41429-025-00838-3","url":null,"abstract":"The screening of antibiotics derived from microbial resources to combat vancomycin-resistant enterococci (VRE) revealed that a culture of marine-derived Peribacillus sp. KDM594 exhibited significant therapeutic efficacy in an infected in vivo-mimic silkworm model. Bioassay-guided purification led to the isolation of micrococcins P1 (1) and P2 (2), which exhibited potent antimicrobial activities against Gram-positive bacteria, including VRE, methicillin-resistant Staphylococcus aureus (MRSA), and Mycobacterium spp., with MIC values ranging from 0.25 to 8.0 µg ml-1 using the microdilution method. In the silkworm models infected with VRE or MRSA, 1 and 2 exerted moderate therapeutic effects, with ED50 values ranging from 3.2 to 51 µg larva-1 g-1. Furthermore, a pharmacokinetic analysis revealed that 2 was metabolized to 1 in the silkworm hemolymph, and their elimination half-lives were 3.2 and 3.0 h, respectively. These results suggest that micrococcins are promising lead compounds for the development of anti-VRE and MRSA drugs.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":"481-487"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144276748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

"Global divide in carbapenem resistance and hypervirulence of Klebsiella pneumonia: comparing trends in India and developed nations"- a comprehensive review. “克雷伯菌肺炎碳青霉烯耐药性和高毒力的全球差异：比较印度和发达国家的趋势”——一篇综合综述。

IF 2.7 4区医学

Journal of Antibiotics Pub Date : 2025-07-01 Epub Date: 2025-06-03 DOI: 10.1038/s41429-025-00833-8

Saloni V Koli, Snehal V Mali, Jisha Geevarghese

{"title":"\"Global divide in carbapenem resistance and hypervirulence of Klebsiella pneumonia: comparing trends in India and developed nations\"- a comprehensive review.","authors":"Saloni V Koli, Snehal V Mali, Jisha Geevarghese","doi":"10.1038/s41429-025-00833-8","DOIUrl":"10.1038/s41429-025-00833-8","url":null,"abstract":"The global health problem of carbapenem-resistant and hypervirulent Klebsiella pneumoniae (CRKP) is exacerbated by the lack of effective treatments and increasing resistance. This study compares the clinical impact, resistance mechanisms, and prevalence of CR-hvKP in industrialized and emerging nations, analyzing 300 papers from national databases and selecting 70 for further examination. Treatment effectiveness, clinical outcomes, resistance mechanisms, and prevalence were assessed in the study, which concentrated on CRKP and hvKP strains that carried virulence genes (rmpA, aerobactin, yersiniabactin) and carbapenemase genes (blaKPC, blaNDM, blaOXA-12). With 25% of studies identifying blaOXA-48, 30% reporting blaKPC, and 45% tying blaNDM to resistance, analysis showed β-lactamase genes are mostly responsible for carbapenem resistance in Klebsiella pneumoniae strains. Resistance rates for CR-hvKP range from 4.4% to 100% in India, where the condition is disproportionately common. Clinical results are alarming: hospital-admitted patients had death rates ranging from 30% to 60% and morbidity rates as high as 90%. The study emphasizes how plasmid-mediated resistance, immune evasion mechanisms, and enhanced biofilm development contribute to the pathophysiology of CR-hvKP, making therapy more difficult. Urgent action is needed to battle CR-hvKP and lessen its catastrophic clinical impact, as it is more common in India than developed countries.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":"457-471"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tuberculosis drug development; fluoroquinolone structural tailoring. 结核病药物开发；氟喹诺酮结构裁剪。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-07-01 DOI: 10.1038/s41429-025-00839-2

Alicia M Gutiérrez-Mauricio, Juan Valentín Trujillo-Paez, Luis Alberto Trejo-Martinez, Bruno Rivas-Santiago, Paola Pérez-García, Saúl Noriega, Juan Ernesto López-Ramos, Jaime Cardoso-Ortiz, Adrián Rodríguez-Carlos

{"title":"Tuberculosis drug development; fluoroquinolone structural tailoring.","authors":"Alicia M Gutiérrez-Mauricio, Juan Valentín Trujillo-Paez, Luis Alberto Trejo-Martinez, Bruno Rivas-Santiago, Paola Pérez-García, Saúl Noriega, Juan Ernesto López-Ramos, Jaime Cardoso-Ortiz, Adrián Rodríguez-Carlos","doi":"10.1038/s41429-025-00839-2","DOIUrl":"https://doi.org/10.1038/s41429-025-00839-2","url":null,"abstract":"Tuberculosis (TB) is a contagious infectious disease caused by the bacillus Mycobacterium tuberculosis (Mtb). It is transmitted through small particles in the air (<5 µm) expelled by active tuberculosis patients; when inhaled by a new host, they can potentially cause infection. Nowadays, TB is still the major cause of morbidity and mortality by a single infectious agent, this is further exacerbated by the worldwide emergence of multidrug-resistant strains of Mtb. Thus, effective methods of diagnosis, prophylaxis, and new pharmacological therapies must be carried out in order to control this disease. Fluoroquinolones (FQ) are synthetic antibiotics with a broad spectrum against Gram-negative and Gram-positive bacteria, including M. tuberculosis. The treatment with FQ plays an important role in managing drug-resistant tuberculosis. Modifications on FQ structure have been extensively studied, thereby, four generations of FQ have emerged having a broad spectrum of antibacterial properties. These modifications improve the overall efficiency of FQ by increasing tissue penetration, reducing side effects, and addressing emerging bacterial resistance. In this scenario, current trends on FQ research have focused on new synthetic approaches that allow fluoroquinolones to address the worldwide issue of multidrug-resistant tuberculosis. The aim of this review is to highlight the overall effects of newly synthesized FQ molecules having antitubercular activity.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144546247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survival strategies of imipenem-resistant P. aeruginosa: a comparative analysis of surface-bound biofilms and unbound aggregates. 耐亚胺培南铜绿假单胞菌的生存策略：表面结合生物膜和非结合聚集体的比较分析。

IF 2.7 4区医学

Journal of Antibiotics Pub Date : 2025-07-01 Epub Date: 2025-06-06 DOI: 10.1038/s41429-025-00834-7

Mahwish Saleem, Mona Siddiqui, Hafiza Noor Ul Huda, Shaista Urooj, Bushra Jabeen, Fouzia Zeeshan Khan, Yasir Raza, Nisar Ahmed Shar, Saeed Khan, Ayaz Ahemd, Zulfiqar Ali Mirani

{"title":"Survival strategies of imipenem-resistant P. aeruginosa: a comparative analysis of surface-bound biofilms and unbound aggregates.","authors":"Mahwish Saleem, Mona Siddiqui, Hafiza Noor Ul Huda, Shaista Urooj, Bushra Jabeen, Fouzia Zeeshan Khan, Yasir Raza, Nisar Ahmed Shar, Saeed Khan, Ayaz Ahemd, Zulfiqar Ali Mirani","doi":"10.1038/s41429-025-00834-7","DOIUrl":"10.1038/s41429-025-00834-7","url":null,"abstract":"This study investigated imipenem-resistant Pseudomonas aeruginosa (P. aeruginosa) isolates recovered from various ready-to-eat food items. Isolates were identified as P. aeruginosa based on growth on selective P-Pseudomonas media and confirmed by PCR amplification of the oprI and oprL genes. These isolates formed biofilms under laboratory conditions at 35 °C in Tryptic Soy Broth (TSB). The biofilms were induced by a sub-inhibitory dose of imipenem. Two types of biofilm aggregates were observed: surface-bound biofilms and unbound cell aggregates. Surface-bound biofilms appeared after 48 h of incubation and reached maximum biomass after 96 h. Unbound aggregates were observed after 72 h of incubation. The biomass of aggregates was measured using a crystal violet binding assay. Further characterization revealed two types of unbound or floating aggregates: aggregates detached from surface-bound biofilms and spontaneously formed cell clusters. Both aggregate types exhibited similar imipenem resistance profiles. Comparative analysis of surface-bound and unbound biofilm cells revealed that surface-bound cells were more hydrophobic and relatively more resistant to high temperatures. Both types of aggregates survived at 80 °C for 12 h. Atomic force microscopy showed that surface-bound P. aeruginosa cells were stiffer, with an average force constant of 56.36 ± 5.21 pN nm-1, compared to cells from unbound aggregates [44.55 ± 4.87 pN nm-1]. Similarly, surface-bound cells exhibited greater adhesiveness, with an average adhesion force of 553.25 ± 62.18 pN, whereas cells from floating aggregates demonstrated lower adhesion force values, averaging 451.81 ± 58.32 pN.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":"500-510"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144250959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rare distribution of butenolide-type signaling molecules among Streptomyces strains and functional importance as inducing factors for secondary metabolite production in Streptomyces rochei 7434AN4. 丁烯内酯型信号分子在链霉菌中的罕见分布及其作为罗氏链霉菌7434AN4次生代谢物产生诱导因子的功能重要性

IF 2.7 4区医学

Journal of Antibiotics Pub Date : 2025-07-01 Epub Date: 2025-06-12 DOI: 10.1038/s41429-025-00840-9

Asahi Hirata, Miho Sumiyoshi, Hazuki Fujita, Momoko Akimoto, Mary Hannah Rose A Padayao, Yuto Eguchi, Maki Matsuura, Miyuki Otsuka, Kuninobu Inada, Aiko Teshima, Kenji Arakawa

{"title":"Rare distribution of butenolide-type signaling molecules among Streptomyces strains and functional importance as inducing factors for secondary metabolite production in Streptomyces rochei 7434AN4.","authors":"Asahi Hirata, Miho Sumiyoshi, Hazuki Fujita, Momoko Akimoto, Mary Hannah Rose A Padayao, Yuto Eguchi, Maki Matsuura, Miyuki Otsuka, Kuninobu Inada, Aiko Teshima, Kenji Arakawa","doi":"10.1038/s41429-025-00840-9","DOIUrl":"10.1038/s41429-025-00840-9","url":null,"abstract":"Streptomyces rochei 7434AN4 produces two structurally unrelated polyketide antibiotics, lankacidin (LC) and lankamycin (LM), and their biosynthesis is tightly controlled by 2,3-disubstituted butenolide-type signaling molecules SRB1 and SRB2. We here investigated the distribution of 2,3-disubstituted butenolides (SRB-type butenolides) among randomly selected 122 Streptomyces strains using two approaches; (1) feeding of their culture extracts into an srrX-deficient strain KA20 of S. rochei, and (2) co-fermentation with strain KA20. All the randomly selected donor strains, except for Streptomyces cellostaticus (a LC and LM producer), failed to restore LC and LM production in strain KA20. These findings strongly revealed the rare distribution of SRB-type butenolide molecules in Streptomyces species. One of the SRB-type butenolide, SAB1, an inducing molecule for nikkomycin production in Streptomyces ansochromogenes, was unable to restore antibiotic production in strain KA20 even at 1 mM concentration. Furthermore, we noticed the accumulation of 4-dehydroxy-SRB1 as a novel compound when SRB1 was fed into strain KA20. Purified 4-dehydroxy-SRB1 has no inducing activity of antibiotic production in strain KA20 even at 1000-fold higher concentration (50 µM) against a minimum inducing concentration of natural SRB1 (40 nM). These findings suggested the importance of the length of a hydrocarbon chain attached at C-2 and a hydroxyl group at C-4 for inducing activity in S. rochei.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":"488-499"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12301235/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144287140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Evaluation of lung epithelial lining fluid concentrations of lascufloxacin against Streptococcus pneumoniae in a hollow-fiber infection model. 更正：在中空纤维感染模型中评估肺上皮内层液中拉库沙星抗肺炎链球菌的浓度。

IF 2.7 4区医学

Journal of Antibiotics Pub Date : 2025-07-01 DOI: 10.1038/s41429-025-00837-4

Haruka Nakagawa Kamura, Tetsuo Yamaguchi, Toshihiro Kasama, Yukitaka Hayashi, Masakaze Hamada, Kazuaki Matsumoto, Ryo Miyake, Yoshikazu Ishii

引用次数: 0

New antimicrobial butanolides from a deep sea-derived fungus Aspergillus tabacinus LW82. 深海衍生真菌烟曲霉LW82的新型抗菌丁烷内酯。

IF 2.7 4区医学

Journal of Antibiotics Pub Date : 2025-07-01 Epub Date: 2025-06-09 DOI: 10.1038/s41429-025-00836-5

Yu Tu, Jinxin Zhang, Lei Cai, Ling Liu

引用次数: 0

Isolation and structure determination of streptospherins B-F, novel cancer stem cell inhibitors, produced by Streptomyces sp. KUSC-240. Streptomyces sp. KUSC-240新型肿瘤干细胞抑制剂streptospherin B-F的分离与结构分析

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2025-06-19 DOI: 10.1038/s41429-025-00843-6

Morihiro Shibasaki, Hiroaki Ikeda, Ami Mimura, Junko Hashimoto, Taiki Suo, Takefumi Kuranaga, Kazuo Shin-Ya, Masaya Imoto, Hideaki Kakeya

{"title":"Isolation and structure determination of streptospherins B-F, novel cancer stem cell inhibitors, produced by Streptomyces sp. KUSC-240.","authors":"Morihiro Shibasaki, Hiroaki Ikeda, Ami Mimura, Junko Hashimoto, Taiki Suo, Takefumi Kuranaga, Kazuo Shin-Ya, Masaya Imoto, Hideaki Kakeya","doi":"10.1038/s41429-025-00843-6","DOIUrl":"10.1038/s41429-025-00843-6","url":null,"abstract":"Streptospherin A (1), which has pentasubstituted benzene and tetrahydropyran moieties, was recently isolated in our laboratory from Streptomyces sp. KUSC-240 as a novel inhibitor of cancer stem cell (CSC) sphere formation. Given the potential of CSCs as target for cancer therapy because of their high malignancy, identification of CSC inhibitors is urgently needed. The chemical structure and biological activity of 1 prompted us to isolate other derivatives from Streptomyces sp. KUSC-240, leading here to the identification of new streptospherins B-F (2-6). Their planar structures were determined by HR-ESI-MS and NMR analyses. The absolute configuration of 4 was proposed by using a modified Mosher's method, acetonide formation, and the J-based configuration analysis (JBCA) method. The absolute configurations of 3 and 5 were also determined by using ECD spectra and comparison with that of 4. Analysis of the whole genome sequence of the producing strain suggested a plausible biosynthesis pathway for 3-5. Compounds 2-6 inhibited CSC sphere formation and suppressed CSC growth, indicating that streptospherins are promising seed compounds for CSC inhibitors for cancer chemotherapy.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144334519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0