Journal of Antibiotics最新文献_第3页

A new cannabigerolic acid derivative and its unprenylated precursor produced through the reconstitution of cannabinoid biosynthesis in Streptomyces 一种新的大麻酚酸衍生物及其未烯丙基化前体通过重组大麻素在链霉菌中的生物合成。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2024-12-03 DOI: 10.1038/s41429-024-00793-5

Kei Kudo, Takehiro Nishimura, Kei Miyako, Hikaru Suenaga, Kazuo Shin-ya

引用次数: 0

Isolation and identification of three new isomer impurities in milbemycin oxime drug substance 米尔霉素肟原料药中三个新的异构体杂质的分离鉴定。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2024-11-28 DOI: 10.1038/s41429-024-00791-7

Xiaohan Ren, Jianwei Lu, Yefei Wu, Shaoyong Zhang, Huan Qi, Hui Zhang, Jidong Wang, Linghui Zheng

引用次数: 0

Acknowledgments 致谢

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2024-11-27 DOI: 10.1038/s41429-024-00779-3

Kazuro Shiomi

引用次数: 0

Identification of nanaomycin A and its analogs by a newly established screening method for functional inhibitors of the type IX secretion system in Porphyromonas gingivalis 通过新建立的牙龈卟啉单胞菌 IX 型分泌系统功能抑制剂筛选方法鉴定纳诺霉素 A 及其类似物。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2024-11-22 DOI: 10.1038/s41429-024-00790-8

Yuko Sasaki, Takehiro Matsuo, Yoshihiro Watanabe, Masato Iwatsuki, Yuki Inahashi, Satoshi Nishida, Mariko Naito, Mikio Shoji

{"title":"Identification of nanaomycin A and its analogs by a newly established screening method for functional inhibitors of the type IX secretion system in Porphyromonas gingivalis","authors":"Yuko Sasaki, Takehiro Matsuo, Yoshihiro Watanabe, Masato Iwatsuki, Yuki Inahashi, Satoshi Nishida, Mariko Naito, Mikio Shoji","doi":"10.1038/s41429-024-00790-8","DOIUrl":"10.1038/s41429-024-00790-8","url":null,"abstract":"Porphyromonas gingivalis, a Gram-negative anaerobic bacterium, is a key pathogen in chronic periodontitis. P. gingivalis has a type IX secretion system (T9SS) that secretes highly hydrolytic proteinases called gingipains for obtaining peptides as an energy source. Although most T9SS-related proteins have been identified, no specific inhibitor of T9SS has been reported. To screen T9SS inhibitors, we focused on and characterized a minimal liquid medium called mC medium that contains milk casein as the sole protein source. We found that P. gingivalis wild-type strain ATCC 33277 caused cloudiness of mC medium without growth. In mC medium, an alkylating agent, iodoacetamide (IAM) that is an inhibitor of gingipains, and a protonophore, carbonyl cyanide 3-chlorophenylhydrazone (CCCP) that dissipates the proton motive force required for T9SS-mediated secretion, clearly inhibited the increase in turbidity. Moreover, neither the gingipain-null mutant nor the T9SS-deficient mutant caused mC medium cloudiness, suggesting that mC medium cloudiness is dependent on gingipain activity and T9SS. These results indicated that mC medium can be used to assess P. gingivalis gingipain activity and its functional T9SS. Using an assay system with mC medium, we discovered that OM-173αA and OM-173βA in the Ōmura Natural Compound Library and nanaomycin A were probable T9SS inhibitors. The compounds need to be further investigated as tools for analyzing T9SS and as potential therapeutic agents for periodontal disease.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":"78 2","pages":"90-105"},"PeriodicalIF":2.1,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41429-024-00790-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of new AMR drugs targeting modulators of antimicrobial activity using in vivo silkworm screening systems 利用体内家蚕筛选系统发现以抗菌活性调节剂为目标的新型 AMR 药物。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2024-11-14 DOI: 10.1038/s41429-024-00788-2

Fumiaki Tabuchi, Kazuhiro Mikami, Masanobu Miyauchi, Kazuhisa Sekimizu, Atsushi Miyashita

{"title":"Discovery of new AMR drugs targeting modulators of antimicrobial activity using in vivo silkworm screening systems","authors":"Fumiaki Tabuchi, Kazuhiro Mikami, Masanobu Miyauchi, Kazuhisa Sekimizu, Atsushi Miyashita","doi":"10.1038/s41429-024-00788-2","DOIUrl":"10.1038/s41429-024-00788-2","url":null,"abstract":"Global concerns about drug-resistant bacteria have underscored the need for new antimicrobial drugs. Emerging strategies in drug discovery include considering the third factors that influence drug activity. These factors include host-derived elements, adjuvants, and drug combinations, which are crucial in regulating antimicrobial efficacy. Traditional in vivo assessments have relied on animal models to study drug absorption, distribution, metabolism, excretion, and toxicity (ADMET). Alternative models, such as silkworms, are being explored to overcome the ethical and financial barriers associated with mammalian models. The silkworm has been proven effective in evaluating ADMET and in highlighting the therapeutic potential enhanced by third factors. Host factors (either mammalian or non-mammalian) enhance the antimicrobial activity of antimicrobial agents such as lysocin E. Additionally, using d-cycloserine to potentiate vancomycin has successfully combated vancomycin-resistant infections in silkworms. Leveraging silkworms in drug discovery could establish a novel screening method incorporating interactions with third factors, whether host related or non-host-related, thus promising new pathways for identifying antimicrobial drugs with unique mechanisms of action.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":"78 2","pages":"69-77"},"PeriodicalIF":2.1,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11769840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142632972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-activity relationship studies of ME1111, a novel antifungal agent for topical treatment of onychomycosis 用于局部治疗甲癣的新型抗真菌剂 ME1111 的结构-活性关系研究。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2024-11-14 DOI: 10.1038/s41429-024-00789-1

Naomi Takei-Masuda, Maiko Iida, Makoto Ohyama, Kaori Kaneda, Kenji Ueda, Yuji Tabata

引用次数: 0

Celludinone C, a new dihydroisobenzofuran isolated from Talaromyces cellulolyticus BF-0307 Celludinone C，一种从纤维素溶解塔拉酵母菌 BF-0307 中分离出来的新型二氢异苯并呋喃。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2024-11-14 DOI: 10.1038/s41429-024-00785-5

Reiko Seki, Kenichiro Nagai, Keisuke Kobayashi, Satoru Shigeno, Tatsuya Shirahata, Yoshinori Kobayashi, Taichi Ohshiro, Hiroshi Tomoda

引用次数: 0

Antimicrobial activity of α/β hybrid peptides incorporating tBu-β3,3Ac6c against methicillin-resistant Staphylococcus aureus 含有 tBu-β3,3Ac6c 的 α/β 杂交肽对耐甲氧西林金黄色葡萄球菌的抗菌活性。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2024-10-29 DOI: 10.1038/s41429-024-00773-9

Aminur Rahman Sarkar, Jyoti Kumari, Arti Rathore, Rubina Chowdhary, Rakshit Manhas, Shifa Firdous, Avisek Mahapa, Rajkishor Rai

{"title":"Antimicrobial activity of α/β hybrid peptides incorporating tBu-β3,3Ac6c against methicillin-resistant Staphylococcus aureus","authors":"Aminur Rahman Sarkar, Jyoti Kumari, Arti Rathore, Rubina Chowdhary, Rakshit Manhas, Shifa Firdous, Avisek Mahapa, Rajkishor Rai","doi":"10.1038/s41429-024-00773-9","DOIUrl":"10.1038/s41429-024-00773-9","url":null,"abstract":"The incorporation of β-amino acids into peptides is a promising approach to develop proteolytically stable therapeutic agents. Short α/β hybrid peptides containing tBu-β3,3Ac6cː H2N-Lys-tBu-β3,3Ac6c-PEA, P1; H2N-Orn-tBu-β3,3Ac6c-PEA, P2; H2N-Arg-tBu-β3,3Ac6c-PEA, P3; LA-Lys-tBu-β3,3Ac6c-PEA, P4; LA-Orn-tBu-β3,3Ac6c-PEA, P5; LA-Arg-tBu-β3,3Ac6c-PEA, P6; LAu-Lys-tBu-β3,3Ac6c-PEA, P7; LAu-Orn-tBu-β3,3Ac6c-PEA, P8; and LAu-Arg-tBu-β3,3Ac6c-PEA, P9 were prepared. The antimicrobial efficacies of all the peptides were evaluated against ESKAPE pathogens, along with a small panel of multi-drug resistant (MDR) clinical isolates of S. aureus. Among all the peptides, P4, P6, and P7 showed significant efficacies against P. aeruginosa, S. aureus, and MRSA with an MIC value ranging from 6.25 to 12.5 μM. Further, in vitro, anti-staphylococcal assessment with their antimicrobial synergy of the peptides P4, P6, and P7 was carried out against MRSA, due to its better efficacy. The peptides P6 and P7 exhibited MRSA biofilm inhibition of 70% and 77%, respectively, at 4×MIC concentration. At its MIC concentration, about 19% hemolysis was observed for P4, P6, and P7.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":"77 12","pages":"794-801"},"PeriodicalIF":2.1,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thiazoplanomicin, a new thiazolyl peptide antibiotic from the leaf-litter actinomycete Actinoplanes sp. MM794L-181F6 来自叶片放线菌 MM794L-181F6 的新型噻唑肽抗生素 Thiazoplanomicin。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2024-10-28 DOI: 10.1038/s41429-024-00783-7

Yasuhiro Takehana, Hideyuki Muramatsu, Masaki Hatano, Yoshimasa Ishizaki, Maya Umekita, Yuko Shibuya, Chigusa Hayashi, Tomoyuki Kimura, Toshifumi Takeuchi, Ken Shimuta, Ryuichi Sawa, Masayuki Igarashi

{"title":"Thiazoplanomicin, a new thiazolyl peptide antibiotic from the leaf-litter actinomycete Actinoplanes sp. MM794L-181F6","authors":"Yasuhiro Takehana, Hideyuki Muramatsu, Masaki Hatano, Yoshimasa Ishizaki, Maya Umekita, Yuko Shibuya, Chigusa Hayashi, Tomoyuki Kimura, Toshifumi Takeuchi, Ken Shimuta, Ryuichi Sawa, Masayuki Igarashi","doi":"10.1038/s41429-024-00783-7","DOIUrl":"10.1038/s41429-024-00783-7","url":null,"abstract":"A new bioactive substance was identified from a leaf-litter actinomycete strain by screening for antibacterial activity against Neisseria gonorrhoeae. The thiazolyl peptide antibiotic, named thiazoplanomicin, was isolated from the secondary metabolites of the leaf-litter actinomycetes Actinoplanes sp. MM794L-181F6 by extraction with n-butanol, silica gel column chromatography, Sephadex LH-20 column chromatography, and preparative HPLC. Thiazoplanomicin was characterized by LC-HR-ESI-MS, NMR, and X-ray analyses, along with analysis of the degradation products and chemical derivatives, and determined to be a nocathiacin-like multiple macrocyclic thiazolyl peptide. Thiazoplanomicin showed potent antimicrobial activity against gonococcal strains, including those resistant to known anti-gonococcal compounds such as telithromycin, azithromycin, and ceftriaxone, with MIC values ranging from 0.0312 to 0.125 µg ml−1. Such anti-gonococcal activity has not been reported on nocathiacin-like thiazolyl peptide antibiotic so far. Similar to other thiazolyl peptide antibiotics, thiazoplanomicin also showed potent antibacterial activity against Gram-positive bacteria with MIC values ranging from 0.0005 to 0.0156 µg ml−1 but showed no antibacterial activity against Escherichia coli.","PeriodicalId":54884,"journal":{"name":"Journal of Antibiotics","volume":"78 1","pages":"14-25"},"PeriodicalIF":2.1,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41429-024-00783-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142523721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sinulariapeptide F, a new peptide from culture broth of marine-derived fungus Simplicillium sp. SCSIO 41222 从海洋源真菌 Simplicillium sp. SCSIO 41222 的培养液中提取的新肽 Sinulariapeptide F。

IF 2.1 4区医学

Journal of Antibiotics Pub Date : 2024-10-25 DOI: 10.1038/s41429-024-00780-w

Yan-Chun He, Meng-Qin Wang, Qing-Qing Tie, Xiao-Wen Huang, Yong-Hong Liu, Yun-Qiu Li, Bin Yang

引用次数: 0