Isolation, structural determination, and antiviral activities of a novel alanine-conjugated polyketide from Talaromyces sp.

IF 2.1 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Antibiotics Pub Date : 2024-05-30 DOI:10.1038/s41429-024-00740-4

Nozomi Mosu, Mitsuki Yasukochi, Shogo Nakajima, Kou Nakamura, Masaya Ogata, Keita Iguchi, Kazuki Kanno, Tomohiro Ishikawa, Kazutoshi Sugita, Hironobu Murakami, Kouji Kuramochi, Tatsuo Saito, Shiro Takeda, Koichi Watashi, Kan Fujino, Shinji Kamisuki

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Abstract

Antiviral agents are highly sought after. In this study, a novel alkylated decalin-type polyketide, alaspelunin, was isolated from the culture broth of the fungus Talaromyces speluncarum FMR 16671, and its structure was determined using spectroscopic analyses (1D/2D NMR and MS). The compound was condensed with alanine, and its absolute configuration was determined using Marfey’s method. Furthermore, the antiviral activity of alaspelunin against various viruses was evaluated, and it was found to be effective against both severe acute respiratory syndrome coronavirus 2 and pseudorabies (Aujeszky’s disease) virus, a pathogen affecting pigs. Our results suggest that this compound is a potential broad-spectrum antiviral agent.

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从塔拉酵母菌（Talaromyces sp.

抗病毒药物备受青睐。本研究从真菌 Talaromyces speluncarum FMR 16671 的培养液中分离出了一种新型烷基化癸醛多酮--alaspelunin，并利用光谱分析（1D/2D NMR 和 MS）确定了其结构。该化合物与丙氨酸缩合，其绝对构型采用马菲法确定。此外，还评估了天冬氨酰胺对各种病毒的抗病毒活性，结果发现它对严重急性呼吸系统综合征冠状病毒 2 和猪伪狂犬病（奥杰茨基病）病毒均有效。我们的研究结果表明，这种化合物是一种潜在的广谱抗病毒剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Antibiotics 医学-免疫学

CiteScore

6.60

自引率

3.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Antibiotics seeks to promote research on antibiotics and related types of biologically active substances and publishes Articles, Review Articles, Brief Communication, Correspondence and other specially commissioned reports. The Journal of Antibiotics accepts papers on biochemical, chemical, microbiological and pharmacological studies. However, studies regarding human therapy do not fall under the journal’s scope. Contributions regarding recently discovered antibiotics and biologically active microbial products are particularly encouraged. Topics of particular interest within the journal''s scope include, but are not limited to, those listed below: Discovery of new antibiotics and related types of biologically active substances Production, isolation, characterization, structural elucidation, chemical synthesis and derivatization, biological activities, mechanisms of action, and structure-activity relationships of antibiotics and related types of biologically active substances Biosynthesis, bioconversion, taxonomy and genetic studies on producing microorganisms, as well as improvement of production of antibiotics and related types of biologically active substances Novel physical, chemical, biochemical, microbiological or pharmacological methods for detection, assay, determination, structural elucidation and evaluation of antibiotics and related types of biologically active substances Newly found properties, mechanisms of action and resistance-development of antibiotics and related types of biologically active substances.