Neurophotonics最新文献

{"title":"Intra-subject test-retest reliability for auditory-evoked functional near-infrared spectroscopy responses: effects of systemic physiology correction.","authors":"Victoria C Sinfield, Dalton Aaker, Abigail Metzger, Yunjie Tong, Maureen J Shader","doi":"10.1117/1.NPh.12.1.015015","DOIUrl":"10.1117/1.NPh.12.1.015015","url":null,"abstract":"<p><strong>Significance: </strong>Functional near-infrared spectroscopy (fNIRS) is a valuable neuroimaging tool for non-invasively measuring hemodynamic changes in response to neural activity, particularly in auditory research. Although fNIRS shows strong test-retest reliability at the group level, individual-subject level reliability is often compromised by systemic noise.</p><p><strong>Aim: </strong>We investigate how correcting for systemic-physiological signals affects reliability in single-subject fNIRS data.</p><p><strong>Approach: </strong>fNIRS data were collected from one participant over 10 sessions during a passive auditory task. Using general linear modeling, six correction approaches were compared: no correction, physiology correction, short-channel correction, short-channel + physiology correction, short-channel + physiology + lag correction, and short-channel + tCCA correction.</p><p><strong>Results: </strong>Intraclass correlation coefficient analysis revealed that physiology correction yielded the highest test-retest reliability score, whereas short-channel correction had the lowest. These results align with previous findings suggesting that global systemic artifacts bolster reliability, and regressing such artifacts enhances the clarity of the observed neuronal response, as supported by visual comparisons of raw and denoised signals.</p><p><strong>Conclusions: </strong>We highlight the impact of correcting for extra-cerebral signals in single-subject auditory research and demonstrate that, while incorporating short channels in fNIRS data collection may reduce reliability, it offers a more accurate representation of the neuronal response.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 1","pages":"015015"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11924667/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polarization optical coherence tomography optoretinography: verifying light-induced photoreceptor outer segment shrinkage and subretinal space expansion.","authors":"Shaiban Ahmed, Taeyoon Son, Guangying Ma, Xincheng Yao","doi":"10.1117/1.NPh.12.1.015005","DOIUrl":"10.1117/1.NPh.12.1.015005","url":null,"abstract":"<p><strong>Significance: </strong>Stimulus-evoked intrinsic optical signal (IOS) changes in retinal photoreceptors are critical for functional optoretinography (ORG). Optical coherence tomography (OCT), with its depth-resolved imaging capability, has been actively explored for IOS imaging of retinal photoreceptors. However, recent OCT studies have reported conflicting results regarding light-induced changes in the photoreceptor outer segments (OSs), with both elongation and shrinkage being observed. These discrepancies may stem from the difficulty in reliably identifying OS boundaries, particularly the inner segment/outer segment (IS/OS) junction and OS tip, as well as potential confusion with subretinal space dynamics. Gaining a better understanding of these light-induced OS changes is essential for accurate interpretation of ORG measurements and for optimizing IOS imaging systems to enhance sensitivity.</p><p><strong>Aim: </strong>We aim to develop a method for the reliable identification of OS boundaries and to verify light-induced photoreceptor OS shrinkage and subretinal space expansion.</p><p><strong>Approach: </strong>We employed a polarization-resolved full-field swept-source optical coherence tomography system capable of sequentially capturing parallel-polarization and cross-polarization OCT signals. The parallel-polarization mode is optimized to detect ballistically reflected photons from well-defined retinal boundaries, such as the IS/OS junction and the photoreceptor tips, whereas cross-polarization primarily captures multiply scattered photons. This differentiation enables parallel-polarization OCT to minimize the interference from scattered photons, enhancing the precision of OCT band quantification.</p><p><strong>Results: </strong>Parallel-polarization OCT revealed photoreceptor OS shrinkage and subretinal space expansion in light conditions compared with dark conditions. Moreover, the overall outer retinal length appeared to swell under light. These observations were consistently confirmed in four healthy adult human subjects.</p><p><strong>Conclusions: </strong>Parallel-polarization OCT provides a reliable method for identifying the IS/OS junction and OS tip, confirming light-induced photoreceptor OS shrinkage and subretinal space expansion.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 1","pages":"015005"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11770343/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiplexed fiber meta-tip-based circular polarimetry for label-free pathological analysis of ischemic stroke.","authors":"Wenlin Luan, Qingcheng Song, Quancheng Cheng, Chunhua Chen, Xia Yu","doi":"10.1117/1.NPh.12.1.015012","DOIUrl":"10.1117/1.NPh.12.1.015012","url":null,"abstract":"<p><strong>Significance: </strong>We present an optical technology for a full-process label-free method for brain slice screening. This proposed multiplexed circular polarimetric method has the advantages of simple operation and high accuracy which may provide easily accessible evidence for the future diagnosis of related diseases.</p><p><strong>Aim: </strong>One of its missions is to provide a quantifiable, reproducible analysis methodology that can replace or supplement traditional qualitative, subjective pathological analysis.</p><p><strong>Approach: </strong>A label-free, sensitive, and rapid circular polarimetric method based on a multiplexed optical fiber meta-tip is proposed for the digital pathology of ischemic stroke. Polarization information of forward-scattered light is used to identify pathological variations of axon distribution in ischemic stroke tissues. The newly designed optical fiber meta-tip with four channels offers miniature illumination in the multiplexed circular polarimetry method.</p><p><strong>Results: </strong>Our automated approach achieves more than 90% area under the curve in classifying ischemic stroke brain tissue in around 1 min.</p><p><strong>Conclusions: </strong>The high-sensitivity and label-free circular polarimetric method based on the multiplexed optical fiber meta-tip renders its potential for rapid digital pathology of various diseases. It will empower the application of digital pathology in future disease diagnosis by quantitatively introducing a new reliable data modality without altering currently established processes.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 1","pages":"015012"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11888776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143588121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced spectral resolution and reduced acquisition time in fiber-based wavelength-swept source Raman spectroscopy.","authors":"Elahe Parham, Maxime Tousignant-Tremblay, Mireille Quémener, Martin Parent, Daniel C Côté","doi":"10.1117/1.NPh.12.1.015014","DOIUrl":"10.1117/1.NPh.12.1.015014","url":null,"abstract":"<p><strong>Significance: </strong>We introduce a fast Raman spectroscopy (SSRS) system that reduces acquisition time and enhances data quality, providing a breakthrough in SSRS for real-time applications. We demonstrate its utility in differentiating brain tissue regions based on lipid and protein content.</p><p><strong>Aim: </strong>Our primary goal was to develop a fast SSRS system that enables rapid data acquisition for <i>in vivo</i> applications. We aimed to investigate its effectiveness in differentiating brain tissue types by analyzing lipid and protein content, ultimately enhancing classification accuracy and supporting advancements in medical diagnostics.</p><p><strong>Approach: </strong>We implemented an optimized circuit and signal processing technique to reduce high-frequency noise and improve signal-to-noise ratio. Brain tissue measurements were validated against staining models, and classification accuracy was tested with principal component analysis (PCA) and support vector machine (SVM).</p><p><strong>Results: </strong>Our SSRS system captures spectra in 1 s which is significantly faster than similar systems. This rapid method enables real-time monitoring and accurate classification of brain regions based on lipid-protein content, confirmed by neurofilament and Nissl staining correlations ( <math> <mrow> <msup><mrow><mi>R</mi></mrow> <mrow><mn>2</mn></mrow> </msup> <mo>=</mo> <mn>0.75</mn></mrow> </math> and 0.55, respectively). Tissue classification showed 80.20% accuracy using spectral intensity at the wavenumbers associated with C-H, <math> <mrow><msub><mi>CH</mi> <mn>3</mn></msub> </mrow> </math> , and <math> <mrow><msub><mi>CH</mi> <mn>2</mn></msub> </mrow> </math> vibrations and 81.23% accuracy using PCA-derived features (PC1, PC2, and PC3).</p><p><strong>Conclusions: </strong>The fast-SSRS system marks a significant advance in Raman spectroscopy, improving speed and data quality. Our setup captures finer spectral details, facilitating reliable differentiation of tissue types, as verified by staining methods and PCA. This method shows promise for real-time tissue analysis and medical diagnostics, outperforming traditional Raman techniques in speed and data throughput.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 1","pages":"015014"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viscocohesive hyaluronan gel enhances stability of intravital multiphoton imaging with subcellular resolution.","authors":"Ryan A Morton, Tyson N Kim","doi":"10.1117/1.NPh.12.S1.S14602","DOIUrl":"10.1117/1.NPh.12.S1.S14602","url":null,"abstract":"<p><p>Multiphoton microscopy (MPM) has become a preferred technique for intravital imaging deep in living tissues with subcellular detail, where resolution and working depths are typically optimized utilizing high numerical aperture, water-immersion objectives with long focusing distances. However, this approach requires the maintenance of water between the specimen and the objective lens, which can be challenging or impossible for many intravital preparations with complex tissues and spatial arrangements. We introduce the novel use of cohesive hyaluronan gel (HG) as an immersion medium that can be used in place of water within existing optical setups to enable multiphoton imaging with equivalent quality and far superior stability. We characterize and compare imaging performance, longevity, and feasibility of preparations in various configurations. This combination of HG with MPM is highly accessible and opens the doors to new intravital imaging applications.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 Suppl 1","pages":"S14602"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11582905/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurovascular coupling over cortical brain areas and resting state network connectivity with and without rigidified carotid artery.","authors":"Marleen E Bakker, Cong Zhang, Matthieu P Vanni, Frédéric Lesage","doi":"10.1117/1.NPh.12.S1.S14606","DOIUrl":"10.1117/1.NPh.12.S1.S14606","url":null,"abstract":"<p><strong>Significance: </strong>Neurovascular coupling (NVC) is key to research as hemodynamics can reflect neuronal activation and is often used in studies regarding the resting state network (RSN). However, several circumstances, including diseases that reduce blood vessel elasticity, can diminish NVC. In these cases, hemodynamic proxies might not accurately reflect the neuronal RSN.</p><p><strong>Aim: </strong>We aim to investigate in resting state if (1) NVC differs over brain regions, (2) NVC remains intact with a mild rigidification of the carotid artery, (3) hemodynamic-based RSN reflects neuronal-based RSN, and (4) RSN differs with a mildly rigidified artery.</p><p><strong>Approach: </strong>We rigidified the right common carotid artery of mice ( <math><mrow><mi>n</mi> <mo>=</mo> <mn>15</mn></mrow> </math> ) by applying a <math> <mrow> <msub><mrow><mi>CaCl</mi></mrow> <mrow><mn>2</mn></mrow> </msub> </mrow> </math> -soaked cloth to it (NaCl for Sham, <math><mrow><mi>n</mi> <mo>=</mo> <mn>17</mn></mrow> </math> ). With simultaneous GCaMP and intrinsic optical imaging, we compared neuronal activation to hemodynamic changes over the entire cortex.</p><p><strong>Results: </strong>NVC parameters did not differ between the CaCl and Sham groups. Likewise, GCaMP and hemodynamic RSN showed similar connections in both groups. However, the parameters of NVC differed over brain regions. Retrosplenial regions had a slower response and a higher HbR peak than sensory and visual regions, and the motor cortex showed less HbO influx than sensory and visual regions.</p><p><strong>Conclusions: </strong>NVC in a resting state differs over brain regions but is not altered by mild rigidification of the carotid artery.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 Suppl 1","pages":"S14606"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11792086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Important role of the right hemisphere in post-stroke cognitive impairment: a functional near-infrared spectroscopy study.","authors":"Yinan Ai, Yu Zhang, Fang Zheng, Haojie Hu, Mingyu Yin, Ziying Ye, Haiqing Zheng, Liying Zhang, Xiquan Hu","doi":"10.1117/1.NPh.12.1.015008","DOIUrl":"10.1117/1.NPh.12.1.015008","url":null,"abstract":"<p><strong>Significance: </strong>The current neuromodulation treatment for post-stroke cognitive impairment (PSCI) is formulated based on interhemispheric inhibition, which is particularly relevant in the context of motor disorders after stroke. However, the pathological mechanism of PSCI remains unclear, which is completely different from motor disorders. Therefore, exploring the pathological brain characteristics of PSCI can provide a reliable theoretical basis for effective neuromodulation treatment for it.</p><p><strong>Aim: </strong>We explored different functional connectivity (FC) manifestations of PSCI with or without aphasia via functional near-infrared spectroscopy (fNIRS) to provide a pathological basis for the neuromodulation strategy.</p><p><strong>Approach: </strong>We collected cognitive performance and fNIRS data from patients with PSCI without aphasia (PSCI group, <math><mrow><mi>n</mi> <mo>=</mo> <mn>33</mn></mrow> </math> ) and patients with post-stroke aphasia (PSA group, <math><mrow><mi>n</mi> <mo>=</mo> <mn>31</mn></mrow> </math> ), using normal cognition stroke patients (SC group, <math><mrow><mi>n</mi> <mo>=</mo> <mn>32</mn></mrow> </math> ) and healthy subjects (HC group, <math><mrow><mi>n</mi> <mo>=</mo> <mn>31</mn></mrow> </math> ) as controls. Differences in FC among different types of stroke-related cognitive impairment were analyzed.</p><p><strong>Results: </strong>The overall FC in the PSCI group was lower than that in the SC or HC group, and the FCs of the right hemisphere, the right default mode network (DMN), and the right central executive network (CEN) of PSCI patients were significantly lower than those of the left ones. In the PSA group, the FCs of the DMN and CEN were not lower than those in the SC and HC groups, and the FC of the left hemisphere was significantly greater than that of the right hemisphere. In addition, the FC of PSCI patients with right lesions was weaker than that of left lesions, which was closely correlated with the cognitive scale.</p><p><strong>Conclusions: </strong>Unlike the left hemisphere activation strategy commonly used previously, our results suggest that the important role of the right hemisphere may be overlooked in PSCI patients with or without aphasia. Future treatment options and studies could consider focusing on the right hemisphere or bilateral hemispheres.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 1","pages":"015008"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11832076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143442554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex difference in language cognition in the elderly group: a near-infrared spectroscopy study.","authors":"Yizhu Tian, Wenyu Jiang, Mingxi Yang, Di Wu, Xiang Li, Deyu Li, Daifa Wang, Meiyun Xia","doi":"10.1117/1.NPh.12.1.015007","DOIUrl":"10.1117/1.NPh.12.1.015007","url":null,"abstract":"<p><strong>Significance: </strong>There are sex differences in the incidence and prevalence of cognitive disorders, such as Alzheimer's disease. Whether this difference is already present in the preclinical stage of the disease is unclear.</p><p><strong>Aim: </strong>We aim to explore whether there are sex differences in brain functional activities of specific cognitive tasks in the elderly and identify sex-related biomarkers of specific cognitive functions, which may provide important references for the mechanism disclosure and clinical early screening and diagnosis of cognitive disorders.</p><p><strong>Approach: </strong>We measured global cerebral hemoglobin concentrations and connectivity in elderly male ( <math><mrow><mi>n</mi> <mo>=</mo> <mn>45</mn></mrow> </math> ) and female ( <math><mrow><mi>n</mi> <mo>=</mo> <mn>44</mn></mrow> </math> ) groups during the letter and category verbal fluency tasks. The sex differences in activation and connectivity and their relationship with task performance were explored.</p><p><strong>Results: </strong>We found that there is a significant sex difference in connectivity, especially connectivity between the left inferior parietal and right prefrontal and left and right occipital in letter tasks, including the connectivity in parietal, left inferior parietal, and left occipital in category tasks. These connectivities were also significantly negatively correlated with the task performance of male groups.</p><p><strong>Conclusions: </strong>Our results indicated the connectivity between the left inferior parietal and right prefrontal, left and right occipital in letter tasks; the internal connectivity in the parietal; and the connectivity between parietal and the left inferior parietal and right occipital in category tasks may be crucial for verbal assessment of aging males. It is expected that the results will assist in cognitive assessment in the elderly group.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 1","pages":"015007"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11817810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143411458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Voluntary locomotion induces an early and remote hemodynamic decrease in the large cerebral veins.","authors":"Beth Eyre, Kira Shaw, Sheila Francis, Clare Howarth, Jason Berwick","doi":"10.1117/1.NPh.12.S1.S14609","DOIUrl":"10.1117/1.NPh.12.S1.S14609","url":null,"abstract":"<p><strong>Significance: </strong>Behavior regulates dural and cerebral vessels, with spontaneous locomotion inducing dural vessel constriction and increasing stimulus-evoked cerebral hemodynamic responses. It is vital to investigate the function of different vascular network components, surrounding and within the brain, to better understand the role of the neurovascular unit in health and neurodegeneration.</p><p><strong>Aim: </strong>We characterized locomotion-induced hemodynamic responses across vascular compartments of the whisker barrel cortex: artery, vein, parenchyma, draining, and meningeal vein.</p><p><strong>Approach: </strong>Using 2D-OIS, hemodynamic responses during locomotion were recorded in 9- to 12-month-old awake mice: wild-type, Alzheimer's disease (AD), atherosclerosis, or mixed (atherosclerosis/AD) models. Within the somatosensory cortex, responses were taken from pial vessels inside the whisker barrel region [(WBR): \"whisker artery\" and \"whisker vein\"], a large vein from the sagittal sinus adjacent to the WBR (draining vein), and meningeal vessels from the dura mater (which do not penetrate cortical tissue).</p><p><strong>Results: </strong>We demonstrate that locomotion evokes an initial decrease in total hemoglobin (HbT) within the draining vein before the increase in HbT within WBR vessels. The locomotion event size influences the magnitude of the HbT increase in the pial vessels of the WBR but not of the early HbT decrease within the draining veins. Following locomotion onset, an early HbT decrease was also observed in the overlying meningeal vessels, which unlike within the cortex did not go on to exceed baseline HbT levels during the remainder of the locomotion response. We show that locomotion-induced hemodynamic responses are altered in disease in the draining vein and whisker artery, suggesting this could be an important neurodegeneration biomarker.</p><p><strong>Conclusions: </strong>This initial reduction in HbT within the draining and meningeal veins potentially serves as a \"space-saving\" mechanism, allowing for large increases in cortical HbT associated with locomotion. Given this mechanism is impacted by disease, it may provide an important target for vascular-based therapeutic interventions.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 Suppl 1","pages":"S14609"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143702326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Programmable scanning diffuse speckle contrast imaging of cerebral blood flow.","authors":"Faezeh Akbari, Xuhui Liu, Fatemeh Hamedi, Mehrana Mohtasebi, Li Chen, Lei Chen, Guoqiang Yu","doi":"10.1117/1.NPh.12.1.015006","DOIUrl":"10.1117/1.NPh.12.1.015006","url":null,"abstract":"<p><strong>Significance: </strong>Cerebral blood flow (CBF) imaging is crucial for diagnosing cerebrovascular diseases. However, existing large neuroimaging techniques with high cost, low sampling rate, and poor mobility make them unsuitable for continuous and longitudinal CBF monitoring at the bedside.</p><p><strong>Aim: </strong>We aimed to develop a low-cost, portable, programmable scanning diffuse speckle contrast imaging (PS-DSCI) technology for fast, high-density, and depth-sensitive imaging of CBF in rodents.</p><p><strong>Approach: </strong>The PS-DSCI employed a programmable digital micromirror device (DMD) for remote line-shaped laser (785 nm) scanning on tissue surface and synchronized a 2D camera for capturing boundary diffuse laser speckle contrasts. New algorithms were developed to address deformations of line-shaped scanning, thus minimizing CBF reconstruction artifacts. The PS-DSCI was examined in head-simulating phantoms and adult mice.</p><p><strong>Results: </strong>The PS-DSCI enables resolving intralipid particle flow contrasts at different tissue depths. <i>In vivo</i> experiments in adult mice demonstrated the capability of PS-DSCI to image global/regional CBF variations induced by 8% <math> <mrow> <msub><mrow><mi>CO</mi></mrow> <mrow><mn>2</mn></mrow> </msub> </mrow> </math> inhalation and transient carotid artery ligations.</p><p><strong>Conclusions: </strong>Compared with conventional point scanning, line scanning in PS-DSCI significantly increases spatiotemporal resolution. The high sampling rate of PS-DSCI is crucial for capturing rapid CBF changes while high spatial resolution is important for visualizing brain vasculature.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 1","pages":"015006"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11770344/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}