Neurophotonics最新文献

{"title":"The fNIRS glossary project: a consensus-based resource for functional near-infrared spectroscopy terminology.","authors":"Katharina Stute, Louisa K Gossé, Samuel Montero-Hernandez, Guy A Perkins, Meryem A Yücel, Simone Cutini, Turgut Durduran, Ann-Christine Ehlis, Marco Ferrari, Judit Gervain, Rickson C Mesquita, Felipe Orihuela-Espina, Valentina Quaresima, Felix Scholkmann, Ilias Tachtsidis, Alessandro Torricelli, Heidrun Wabnitz, Arjun G Yodh, Stefan A Carp, Hamid Dehghani, Qianqian Fang, Sergio Fantini, Yoko Hoshi, Haijing Niu, Hellmuth Obrig, Franziska Klein, Christina Artemenko, Aahana Bajracharya, Beatrix Barth, Christian Bartkowski, Lénac Borot, Chiara Bulgarelli, David R Busch, Malgorzata Chojak, Jason M DeFreitas, Laura Diprossimo, Thomas Dresler, Aykut Eken, Mahmoud M Elsherif, Lauren L Emberson, Anna Exner, Talukdar Raian Ferdous, Abigail Fiske, Samuel H Forbes, Jessica Gemignani, Christian Gerloff, Ségolène M R Guérin, Edgar Guevara, Antonia F de C Hamilton, S M Hadi Hosseini, Divya Jain, Anastasia N Kerr-German, Haiyan Kong, Agnes Kroczek, Jason K Longhurst, Michael Lührs, Rob J MacLennan, David M A Mehler, Kimberly L Meidenbauer, David Moreau, Murat C Mutlu, Renato Orti, Ishara Paranawithana, Paola Pinti, Ali Rahimpour Jounghani, Vanessa Reindl, Nicholas A Ross, Sara Sanchez-Alonso, Oliver Seidel-Marzi, Mohinish Shukla, Syed A Usama, Musa Talati, Grégoire Vergotte, M Atif Yaqub, Chia-Chuan Yu, Hanieh Zainodini","doi":"10.1117/1.NPh.12.2.027801","DOIUrl":"https://doi.org/10.1117/1.NPh.12.2.027801","url":null,"abstract":"<p><strong>Significance: </strong>A shared understanding of terminology is essential for clear scientific communication and minimizing misconceptions. This is particularly challenging in rapidly expanding, interdisciplinary domains that utilize functional near-infrared spectroscopy (fNIRS), where researchers come from diverse backgrounds and apply their expertise in fields such as engineering, neuroscience, and psychology.</p><p><strong>Aim: </strong>The fNIRS Glossary Project was established to develop a community-sourced glossary covering key fNIRS terms, including those related to the continuous-wave (CW), frequency-domain (FD), and time-domain (TD) NIRS techniques.</p><p><strong>Approach: </strong>The glossary was collaboratively developed by a diverse group of 76 fNIRS researchers, representing a wide range of career stages (from PhD students to experts) and disciplines. This collaborative process, structured across five phases, ensured the glossary's depth and comprehensiveness.</p><p><strong>Results: </strong>The glossary features over 300 terms categorized into six key domains: analysis, experimental design, hardware, neuroscience, mathematics, and physics. It also includes abbreviations, symbols, synonyms, references, alternative definitions, and figures where relevant.</p><p><strong>Conclusions: </strong>The fNIRS glossary provides a community-sourced resource that facilitates education and effective scientific communication within the fNIRS community and related fields. By lowering barriers to learning and engaging with fNIRS, the glossary is poised to benefit a broad spectrum of researchers, including those with limited access to educational resources.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 2","pages":"027801"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12007957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A low-cost FPGA-based approach for pile-up corrected high-speed <i>in vivo</i> FLIM imaging.","authors":"Felipe Velasquez Moros, Dorian Amiet, Rachel M Meister, Alexandra von Faber-Castell, Matthias Wyss, Aiman S Saab, Paul Zbinden, Bruno Weber, Luca Ravotto","doi":"10.1117/1.NPh.12.2.025009","DOIUrl":"https://doi.org/10.1117/1.NPh.12.2.025009","url":null,"abstract":"<p><strong>Significance: </strong>Intensity-based two-photon microscopy is a cornerstone of neuroscience research but lacks the ability to measure concentrations, a pivotal task for longitudinal studies and quantitative comparisons. Fluorescence lifetime imaging (FLIM) based on time-correlated single photon counting (TCSPC) can overcome those limits but suffers from \"pile-up\" distortions at high photon count rates, severely limiting acquisition speed.</p><p><strong>Aim: </strong>We introduce the \"laser period blind time\" (LPBT) method to correct pile-up distortions in photon counting electronics, enabling reliable low-cost TCSPC-FLIM at high count rates.</p><p><strong>Approach: </strong>Using a realistic simulation of the TCSPC data collection, we evaluated the LPBT method's performance <i>in silico</i>. The correction was then implemented on low-cost hardware based on a field programable gate array and validated using <i>in vitro</i>, <i>ex vivo</i>, and <i>in vivo</i> measurements.</p><p><strong>Results: </strong>The LBPT approach achieves <math><mrow><mo><</mo> <mn>3</mn> <mo>%</mo></mrow> </math> error in lifetime measurements at count rates more than 10 times higher than traditional limits, allowing robust FLIM imaging of subsecond metabolite dynamics with subcellular resolution.</p><p><strong>Conclusions: </strong>We enable high-precision, cost-effective FLIM imaging at acquisition speeds comparable with state-of-the-art commercial systems, facilitating the adoption of FLIM in neuroscience and other fields of research needing robust quantitative live imaging solutions.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 2","pages":"025009"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12052397/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144055868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retinal changes detected by diffuse reflectance spectroscopy in parkinsonian monkeys.","authors":"Jonathan Munro, Elahe Parham, Damon DePaoli, Nicolas Lapointe, Cleophace Akitegetse, Shirley Fecteau, Dominic Sauvageau, Thérèse Di Paolo, Daniel C Côté, Martin Parent","doi":"10.1117/1.NPh.12.2.025008","DOIUrl":"https://doi.org/10.1117/1.NPh.12.2.025008","url":null,"abstract":"<p><strong>Significance: </strong>Parkinson's disease (PD) is diagnosed when 50% neurodegeneration has occurred. The retina could provide biomarkers that would allow for earlier diagnosis. Retinal spectroscopy is a technique that could be used to find such biomarkers.</p><p><strong>Aim: </strong>We aimed to find new diagnostic biomarkers for PD following detailed spectral examinations of the retina.</p><p><strong>Approach: </strong>The newly developed Zilia Ocular device was used to perform spectrometric scans of the optic nerve head (ONH) and the retina of four cynomolgus monkeys (<i>Macaca fascicularis</i>) before and after the administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), a neurotoxin used to produce the gold-standard animal model of PD. From the spectrometric data, the blood oximetry was calculated, and the diffuse reflectance spectra (DRS) were analyzed to find variations between the two experimental conditions. Post-mortem analyses were also performed on the retina of the four parkinsonian monkeys and four additional control animals.</p><p><strong>Results: </strong>The analysis of the DRS indicated a lower slope between the 480- and 525-nm wavelengths in both the ONH and the retina. Post-mortem measurements of the retinal layer thicknesses showed that the outer nuclear layer was significantly thinner in MPTP-intoxicated monkeys, compared with controls. Altogether, these results indicate that MPTP altered the optical properties of the ONH and the retina and show that these variations might be explained by MPTP-induced structural changes in the eye fundus, as observed post-mortem.</p><p><strong>Conclusions: </strong>Overall, our results indicate that spectroscopy could be used as a noninvasive method to detect changes in the retina that occur in PD and that such changes could represent retinal biomarkers for improved diagnosis.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 2","pages":"025008"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12052396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NIRSTORM: a Brainstorm extension dedicated to functional near-infrared spectroscopy data analysis, advanced 3D reconstructions, and optimal probe design.","authors":"Édouard Delaire, Thomas Vincent, Zhengchen Cai, Alexis Machado, Laurent Hugueville, Denis Schwartz, Francois Tadel, Raymundo Cassani, Louis Bherer, Jean-Marc Lina, Mélanie Pélégrini-Issac, Christophe Grova","doi":"10.1117/1.NPh.12.2.025011","DOIUrl":"https://doi.org/10.1117/1.NPh.12.2.025011","url":null,"abstract":"<p><strong>Significance: </strong>Understanding the brain's complex functions requires multimodal approaches that combine data from various neuroimaging techniques. Functional near-infrared spectroscopy (fNIRS) offers valuable insights into hemodynamic responses, complementing other modalities such as electroencephalography (EEG), magnetoencephalography (MEG), and magnetic resonance imaging. However, there is a lack of comprehensive and accessible toolboxes able to integrate fNIRS advanced analyses with other modalities. NIRSTORM addresses this gap by offering a unified platform for multimodal neuroimaging analysis.</p><p><strong>Aim: </strong>NIRSTORM aims to provide a user-friendly and comprehensive environment for multimodal analysis while supporting the entire fNIRS analysis pipeline, from experiment planning to the reconstruction of hemodynamic fluctuations on the cortex.</p><p><strong>Approach: </strong>Developed in MATLAB^®, NIRSTORM operates as a Brainstorm plugin, enhancing Brainstorm's capabilities for analyzing fNIRS data. Brainstorm is a widely used, GUI-based software originally designed for statistical analysis and source imaging of EEG and MEG data.</p><p><strong>Results: </strong>NIRSTORM supports conventional fNIRS preprocessing and statistical analyses while introducing new advanced features such as optimal montage for planning optode placement and maximum entropy on the mean (MEM) for reconstructing hemodynamic fluctuations on the cortical surface.</p><p><strong>Conclusion: </strong>As an open-access and user-friendly plugin, NIRSTORM extends Brainstorm's functionality to fNIRS, bridging the gap between EEG/MEG and hemodynamic analyses.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 2","pages":"025011"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144082075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brain-wide 3D neuron detection and mapping with deep learning.","authors":"Yuanyang Liu, Ziyan Gao, Zhehao Xu, Chaoyue Yang, Pei Sun, Longhui Li, Hongbo Jia, Xiaowei Chen, Xiang Liao, Junxia Pan, Meng Wang","doi":"10.1117/1.NPh.12.2.025012","DOIUrl":"10.1117/1.NPh.12.2.025012","url":null,"abstract":"<p><strong>Significance: </strong>Mapping the spatial distribution of specific neurons across the entire brain is essential for understanding the neural circuits associated with various brain functions, which in turn requires automated and reliable neuron detection and mapping techniques.</p><p><strong>Aim: </strong>To accurately identify somatic regions from 3D imaging data and generate reliable soma locations for mapping to diverse brain regions, we introduce NeuronMapper, a brain-wide 3D neuron detection and mapping approach that leverages the power of deep learning.</p><p><strong>Approach: </strong>NeuronMapper is implemented as a four-stage framework encompassing preprocessing, classification, detection, and mapping. Initially, whole-brain imaging data is divided into 3D sub-blocks during the preprocessing phase. A lightweight classification network then identifies the sub-blocks containing somata. Following this, a Video Swin Transformer-based segmentation network delineates the soma regions within the identified sub-blocks. Last, the locations of the somata are extracted and registered with the Allen Brain Atlas for comprehensive whole-brain neuron mapping.</p><p><strong>Results: </strong>Through the accurate detection and localization of somata, we achieved the mapping of somata at the one million level within the mouse brain. Comparative analyses with other soma detection techniques demonstrated that our method exhibits remarkably superior performance for whole-brain 3D soma detection.</p><p><strong>Conclusions: </strong>Our approach has demonstrated its effectiveness in detecting and mapping somata within whole-brain imaging data. This method can serve as a computational tool to facilitate a deeper understanding of the brain's complex networks and functions.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 2","pages":"025012"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12093273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of the brain function characteristics in children with cerebral palsy during walking using functional near-infrared spectroscopy.","authors":"Tengyu Zhang, Gongcheng Xu, Yajie Chang, Zichao Nie, Aiping Sun, Zengyong Li, Ping Xie","doi":"10.1117/1.NPh.12.2.025004","DOIUrl":"10.1117/1.NPh.12.2.025004","url":null,"abstract":"<p><strong>Significance: </strong>Abnormal gait of children with cerebral palsy (CP) is caused by brain damage or developmental defects, exploring the brain's functional characteristics and regulatory mechanisms is essential for rehabilitation.</p><p><strong>Aim: </strong>We aim to study the brain function characteristics in children with CP during walking.</p><p><strong>Approach: </strong>The cortical activation, functional connectivity, information flow, and dynamic state transitions of 17 children with CP and 13 healthy children (HC) were analyzed in the resting and walking states.</p><p><strong>Results: </strong>The motor cortex (MC) of HC is significantly activated in the walking state, whereas both the prefrontal cortex (PFC) and MC of children with CP are significantly activated. The resting brain functional connectivity of children with CP decreased and showed higher global efficiency and modularity and lower clustering coefficients and local efficiency. During walking, the brain network of children with CP was difficult to maintain a stable global high-connectivity state so the local high-connectivity state became the main connectivity state. For children with CP, more brain resources were allocated to the non-dominant MC during walking, whereas more brain resources were allocated to the dominant MC in HC.</p><p><strong>Conclusions: </strong>These indicators reflect the characteristics of brain activation, network connectivity, and information regulation in children with CP, which provide the theoretical basis for targeted rehabilitation treatment.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 2","pages":"025004"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11957398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visible-light optical coherence tomography and its applications.","authors":"Siyu Song, Tristan T Hormel, Yali Jia","doi":"10.1117/1.NPh.12.2.020601","DOIUrl":"https://doi.org/10.1117/1.NPh.12.2.020601","url":null,"abstract":"<p><p>Visible-light optical coherence tomography (vis-OCT) is an emerging OCT technology that uses visible rather than near-infrared illumination and is useful for pre-clinical and clinical imaging. It provides one-micron level axial resolution and distinct scattering and absorption contrast that enables oximetry but requires additional considerations in system implementation and practical settings. We review the development of vis-OCT and demonstrated applications. We also provide insights into prospects and possible technological improvements that may address current challenges.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 2","pages":"020601"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11981582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized laser speckle-based imaging system and methods for deep tissue cerebral blood flow imaging in small rodents.","authors":"Ria Paul, Soumyajit Sarkar, Susweta Das, Shruti D Marathe, Murali Krishnamoorthy, Nixon M Abraham, Hari M Varma","doi":"10.1117/1.NPh.12.2.025017","DOIUrl":"10.1117/1.NPh.12.2.025017","url":null,"abstract":"<p><strong>Significance: </strong>The imaging of cerebral blood flow in small rodents is crucial for a better understanding of brain functions in healthy and diseased conditions. Existing methods often struggle to provide both superficial and deep tissue blood flow measurements in a non-invasive, flexible, and reliable manner, creating a need for an integrated platform that addresses these limitations.</p><p><strong>Aim: </strong>We aim to design and develop a multi-modal laser speckle-based imaging platform and associated algorithms to image superficial and deep tissue cerebral blood flow in small rodents.</p><p><strong>Approach: </strong>A modular design has been adopted to integrate laser speckle contrast imaging and multi-speckle diffuse correlation tomography to a single cerebral blood flow imaging platform for small rodents with an independent module for animal holding and handling. A topographic imaging method, equipped with a filter to remove surface artifacts, was incorporated to image cerebral blood flow changes in response to forepaw and olfactory stimuli activations, with the skull and scalp kept intact.</p><p><strong>Results: </strong>A significant increase in blood flow was found in the olfactory bulbs of mice post-stimulation by various odors ( <math><mrow><mi>p</mi> <mo><</mo> <mn>0.01</mn></mrow> </math> ). Similarly, forepaw stimulation resulted in a significant increase in blood flow in the contralateral side of the somatosensory cortex with the application of the filter for skull and scalp intact, skull intact, and skull removed cases ( <math><mrow><mi>p</mi> <mo><</mo> <mn>0.01</mn></mrow> </math> ).</p><p><strong>Conclusions: </strong>We have validated our system through functional studies, demonstrating its capability to detect enhanced blood flow changes across the olfactory bulbs and somatosensory cortex in rodents with potential for broad applications in preclinical research.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 2","pages":"025017"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12185355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-localized optode-electrode design for multimodal functional near infrared spectroscopy and electroencephalography.","authors":"De'Ja Rogers, Walker Joseph O'Brien, Yuanyuan Gao, Bernhard Zimmermann, Shrey Grover, Yiwen Zhang, Anna Kawai Gaona, Sudan Duwadi, Jessica E Anderson, Laura Carlton, Parisa Rahimi, Parya Y Farzam, Alexander von Lühmann, Robert M G Reinhart, David A Boas, Meryem A Yücel","doi":"10.1117/1.NPh.12.2.025006","DOIUrl":"10.1117/1.NPh.12.2.025006","url":null,"abstract":"<p><strong>Significance: </strong>Neuroscience of the everyday world requires continuous mobile brain imaging in real time and in ecologically valid environments, which aids in directly translating research for human benefit. Combined functional near-infrared spectroscopy (fNIRS) and electroencephalography (EEG) studies have increased in demand, as the combined systems can provide great insights into cortical hemodynamics, neuronal activity, and neurovascular coupling. However, fNIRS-EEG studies remain limited in modularity and portability due to restrictions in combined cap designs, especially for high-density (HD) fNIRS measurements.</p><p><strong>Aim: </strong>We have built and tested custom fNIRS sources that attach to electrodes without decreasing the overall modularity and portability of the probe.</p><p><strong>Approach: </strong>To demonstrate the design's utility, we screened for any potential interference and performed a HD-fNIRS-EEG measurement with co-located opto-electrode positions during a modified Stroop task.</p><p><strong>Results: </strong>No observable interference was present from the fNIRS source optodes in the EEG spectral analysis. The performance, fNIRS, and EEG results of the Stroop task supported the trends from previous research. We observed increased activation with both fNIRS and EEG within the regions of interest.</p><p><strong>Conclusion: </strong>Overall, these results suggest that the co-localization method is a promising approach to multimodal imaging.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 2","pages":"025006"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11978466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shallow-angle intracranial cannula for repeated infusion and <i>in vivo</i> imaging with multiphoton microscopy.","authors":"Steven S Hou, Joyce Yang, Yeseo Kwon, Qi Pian, Yijing Tang, Christine A Dauphinais, Maria Calvo-Rodriguez, Mirna El Khatib, Sergei A Vinogradov, Sava Sakadzic, Brian J Bacskai","doi":"10.1117/1.NPh.12.2.025001","DOIUrl":"10.1117/1.NPh.12.2.025001","url":null,"abstract":"<p><strong>Significance: </strong>Multiphoton microscopy serves as an essential tool for high-resolution imaging of the living mouse brain. To facilitate optical access to the brain during imaging, cranial window surgery is commonly used. However, this procedure restricts physical access above the imaging area and hinders the direct delivery of imaging agents and chemical compounds to the brain.</p><p><strong>Aim: </strong>We aim to develop a method that allows the repeated administration of imaging agents and compounds to the mouse brain while performing <i>in vivo</i> imaging with multiphoton microscopy.</p><p><strong>Approach: </strong>We have developed a cannula delivery system that enables the implantation of a low-profile cannula nearly parallel to the brain surface at angles as shallow as 8 deg while maintaining compatibility with multiphoton microscopy.</p><p><strong>Results: </strong>To validate our shallow-angle cannula approach, we performed direct infusion and imaging of various fluorescent cell markers in the brain. In addition, we successfully demonstrated tracking of degenerating neurons over time in Alzheimer's disease mice using Fluoro-Jade C. Furthermore, we showed longitudinal imaging of the partial pressure of oxygen in brain tissue using a phosphorescent oxygen sensor.</p><p><strong>Conclusions: </strong>Our developed technique should enable a wide range of longitudinal imaging studies in the mouse brain.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"12 2","pages":"025001"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143722523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}