Neurophotonics最新文献

{"title":"Neurophotonics beyond the surface: unmasking the brain’s complexity exploiting optical scattering","authors":"Fei Xia, Caio Vaz Rimoli, Walther Akemann, Cathie Ventalon, Laurent Bourdieu, Sylvain Gigan, Hilton B. de Aguiar","doi":"10.1117/1.nph.11.s1.s11510","DOIUrl":"https://doi.org/10.1117/1.nph.11.s1.s11510","url":null,"abstract":"The intricate nature of the brain necessitates the application of advanced probing techniques to comprehensively study and understand its working mechanisms. Neurophotonics offers minimally invasive methods to probe the brain using optics at cellular and even molecular levels. However, multiple challenges persist, especially concerning imaging depth, field of view, speed, and biocompatibility. A major hindrance to solving these challenges in optics is the scattering nature of the brain. This perspective highlights the potential of complex media optics, a specialized area of study focused on light propagation in materials with intricate heterogeneous optical properties, in advancing and improving neuronal readouts for structural imaging and optical recordings of neuronal activity. Key strategies include wavefront shaping techniques and computational imaging and sensing techniques that exploit scattering properties for enhanced performance. We discuss the potential merger of the two fields as well as potential challenges and perspectives toward longer term in vivo applications.","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"111 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140571444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-photon imaging of excitatory and inhibitory neural response to infrared neural stimulation.","authors":"Peng Fu, Yin Liu, Liang Zhu, Mengqi Wang, Yuan Yu, Fen Yang, Weijie Zhang, Hequn Zhang, Shy Shoham, Anna Wang Roe, Wang Xi","doi":"10.1117/1.NPh.11.2.025003","DOIUrl":"10.1117/1.NPh.11.2.025003","url":null,"abstract":"<p><strong>Significance: </strong>Pulsed infrared neural stimulation (INS, 1875 nm) is an emerging neurostimulation technology that delivers focal pulsed heat to activate functionally specific mesoscale networks and holds promise for clinical application. However, little is known about its effect on excitatory and inhibitory cell types in cerebral cortex.</p><p><strong>Aim: </strong>Estimates of summed population neuronal response time courses provide a potential basis for neural and hemodynamic signals described in other studies.</p><p><strong>Approach: </strong>Using two-photon calcium imaging in mouse somatosensory cortex, we have examined the effect of INS pulse train application on hSyn neurons and mDlx neurons tagged with GCaMP6s.</p><p><strong>Results: </strong>We find that, in anesthetized mice, each INS pulse train reliably induces robust response in hSyn neurons exhibiting positive going responses. Surprisingly, mDlx neurons exhibit negative going responses. Quantification using the index of correlation illustrates responses are reproducible, intensity-dependent, and focal. Also, a contralateral activation is observed when INS applied.</p><p><strong>Conclusions: </strong>In sum, the population of neurons stimulated by INS includes both hSyn and mDlx neurons; within a range of stimulation intensities, this leads to overall excitation in the stimulated population, leading to the previously observed activations at distant post-synaptic sites.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 2","pages":"025003"},"PeriodicalIF":5.3,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11125280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toward a brighter constellation: multiorgan neuroimaging of neural and vascular dynamics in the spinal cord and brain.","authors":"Dmitrijs Celinskis, Christopher J Black, Jeremy Murphy, Adriel Barrios-Anderson, Nina G Friedman, Nathan C Shaner, Carl Y Saab, Manuel Gomez-Ramirez, David A Borton, Christopher I Moore","doi":"10.1117/1.NPh.11.2.024209","DOIUrl":"10.1117/1.NPh.11.2.024209","url":null,"abstract":"<p><strong>Significance: </strong>Pain comprises a complex interaction between motor action and somatosensation that is dependent on dynamic interactions between the brain and spinal cord. This makes understanding pain particularly challenging as it involves rich interactions between many circuits (e.g., neural and vascular) and signaling cascades throughout the body. As such, experimentation on a single region may lead to an incomplete and potentially incorrect understanding of crucial underlying mechanisms.</p><p><strong>Aim: </strong>We aimed to develop and validate tools to enable detailed and extended observation of neural and vascular activity in the brain and spinal cord. The first key set of innovations was targeted to developing novel imaging hardware that addresses the many challenges of multisite imaging. The second key set of innovations was targeted to enabling bioluminescent (BL) imaging, as this approach can address limitations of fluorescent microscopy including photobleaching, phototoxicity, and decreased resolution due to scattering of excitation signals.</p><p><strong>Approach: </strong>We designed 3D-printed brain and spinal cord implants to enable effective surgical implantations and optical access with wearable miniscopes or an open window (e.g., for one- or two-photon microscopy or optogenetic stimulation). We also tested the viability for BL imaging and developed a novel modified miniscope optimized for these signals (BLmini).</p><p><strong>Results: </strong>We describe \"universal\" implants for acute and chronic simultaneous brain-spinal cord imaging and optical stimulation. We further describe successful imaging of BL signals in both foci and a new miniscope, the \"BLmini,\" which has reduced weight, cost, and form-factor relative to standard wearable miniscopes.</p><p><strong>Conclusions: </strong>The combination of 3D-printed implants, advanced imaging tools, and bioluminescence imaging techniques offers a coalition of methods for understanding spinal cord-brain interactions. Our work has the potential for use in future research into neuropathic pain and other sensory disorders and motor behavior.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 2","pages":"024209"},"PeriodicalIF":4.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11079446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in bioluminescent probes for neurobiology.","authors":"Katherine M Townsend, Jennifer A Prescher","doi":"10.1117/1.NPh.11.2.024204","DOIUrl":"10.1117/1.NPh.11.2.024204","url":null,"abstract":"<p><p>Bioluminescence is a popular modality for imaging in living organisms. The platform relies on enzymatically (luciferase) generated light via the oxidation of small molecule luciferins. Since no external light is needed for photon production, there are no concerns with background autofluorescence or photobleaching over time-features that have historically limited other optical readouts. Bioluminescence is thus routinely used for longitudinal tracking across whole animals. Applications in the brain, though, have been more challenging due to a lack of sufficiently bioavailable, bright, and easily multiplexed probes. Recent years have seen the development of designer luciferase and luciferin pairs that address these issues, providing more sensitive and real-time readouts of biochemical features relevant to neurobiology. This review highlights many of the advances in bioluminescent probe design, with a focus on the small molecule light emitter, the luciferin. Specific efforts to improve luciferin pharmacokinetics and tissue-penetrant emission are covered, in addition to applications that such probes have enabled. The continued development of improved bioluminescent probes will aid in illuminating critical neurochemical processes in the brain.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 2","pages":"024204"},"PeriodicalIF":5.3,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10883388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139934234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Method for optimizing imaging parameters to record neuronal and cellular activity at depth with bioluminescence.","authors":"Alexander D Silvagnoli, Kaylee A Taylor, Ashley N Slaviero, Eric D Petersen","doi":"10.1117/1.NPh.11.2.024206","DOIUrl":"10.1117/1.NPh.11.2.024206","url":null,"abstract":"<p><strong>Significance: </strong>Optical imaging has accelerated neuroscience in recent years. Genetically encoded fluorescent activity sensors of calcium, neurotransmitters, and voltage are commonly used for optical recording of neuronal activity. However, fluorescence imaging is limited to superficial regions for <i>in vivo</i> activity imaging, due to photon scattering and absorbance. Bioluminescence imaging offers a promising alternative for achieving activity imaging in deeper brain regions without hardware implanted within the brain. Bioluminescent reporters can be genetically encoded and produce photons without external excitation. The use of enzymatic photon production also enables prolonged imaging sessions without the risk of photobleaching or phototoxicity, making bioluminescence suitable for non-invasive imaging of deep neuronal populations.</p><p><strong>Aim: </strong>To facilitate the adoption of bioluminescent activity imaging, we sought to develop a low cost, simple <i>in vitro</i> method that simulates <i>in vivo</i> conditions to optimize imaging parameters for determining optimal exposure times and optical hardware configurations to determine what frame rates can be captured with an individual lab's imaging hardware with sufficient signal-to-noise ratios without the use of animals prior to starting an <i>in vivo</i> experiment.</p><p><strong>Approach: </strong>We developed an assay for modeling <i>in vivo</i> optical conditions with a brain tissue phantom paired with engineered cells that produce bioluminescence. We then used this assay to limit-test the detection depth versus maximum frame rate for bioluminescence imaging at experimentally relevant tissue depths using off-the-shelf imaging hardware.</p><p><strong>Results: </strong>We developed an assay for modeling <i>in vivo</i> optical conditions with a brain tissue phantom paired with engineered cells that produce bioluminescence. With this method, we demonstrate an effective means for increasing the utility of bioluminescent tools and lowering the barrier to adoption of bioluminescence activity imaging.</p><p><strong>Conclusions: </strong>We demonstrated an improved method for optimizing imaging parameters for activity imaging <i>in vivo</i> with bioluminescent sensors.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 2","pages":"024206"},"PeriodicalIF":5.3,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10976037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140319925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioluminescence as a functional tool for visualizing and controlling neuronal activity <i>in vivo</i>.","authors":"Montserrat Porta-de-la-Riva, Luis-Felipe Morales-Curiel, Adriana Carolina Gonzalez, Michael Krieg","doi":"10.1117/1.NPh.11.2.024203","DOIUrl":"10.1117/1.NPh.11.2.024203","url":null,"abstract":"<p><p>The use of bioluminescence as a reporter for physiology in neuroscience is as old as the discovery of the calcium-dependent photon emission of aequorin. Over the years, luciferases have been largely replaced by fluorescent reporters, but recently, the field has seen a renaissance of bioluminescent probes, catalyzed by unique developments in imaging technology, bioengineering, and biochemistry to produce luciferases with previously unseen colors and intensity. This is not surprising as the advantages of bioluminescence make luciferases very attractive for noninvasive, longitudinal <i>in vivo</i> observations without the need of an excitation light source. Here, we review how the development of dedicated and specific sensor-luciferases afforded, among others, transcranial imaging of calcium and neurotransmitters, or cellular metabolites and physical quantities such as forces and membrane voltage. Further, the increased versatility and light output of luciferases have paved the way for a new field of functional bioluminescence optogenetics, in which the photon emission of the luciferase is coupled to the gating of a photosensor, e.g., a channelrhodopsin and we review how they have been successfully used to engineer synthetic neuronal connections. Finally, we provide a primer to consider important factors in setting up functional bioluminescence experiments, with a particular focus on the genetic model <i>Caenorhabditis elegans</i>, and discuss the leading challenges that the field needs to overcome to regain a competitive advantage over fluorescence modalities. Together, our paper caters to experienced users of bioluminescence as well as novices who would like to experience the advantages of luciferases in their own hand.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 2","pages":"024203"},"PeriodicalIF":5.3,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10861157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139725009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in light patterned optogenetic photostimulation in freely moving mice","authors":"Antonio Lorca-Cámara, François G. C. Blot, Nicolò Accanto","doi":"10.1117/1.nph.11.s1.s11508","DOIUrl":"https://doi.org/10.1117/1.nph.11.s1.s11508","url":null,"abstract":"Optogenetics opened the door to a new era of neuroscience. New optical developments are under way to enable high-resolution neuronal activity imaging and selective photostimulation of neuronal ensembles in freely moving animals. These advancements could allow researchers to interrogate, with cellular precision, functionally relevant neuronal circuits in the framework of naturalistic brain activity. We provide an overview of the current state-of-the-art of imaging and photostimulation in freely moving rodents and present a road map for future optical and engineering developments toward miniaturized microscopes that could reach beyond the currently existing systems.","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"128 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139954670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2023 List of Reviewers","authors":"","doi":"10.1117/1.oe.63.1.010102","DOIUrl":"https://doi.org/10.1117/1.oe.63.1.010102","url":null,"abstract":"Abstract. Thanks to reviewers who served Neurophotonics in 2023.","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"84 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139458199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing the performance potential of speckle contrast optical spectroscopy and diffuse correlation spectroscopy for cerebral blood flow monitoring using Monte Carlo simulations in realistic head geometries.","authors":"Mitchell B Robinson, Tom Y Cheng, Marco Renna, Melissa M Wu, Byungchan Kim, Xiaojun Cheng, David A Boas, Maria Angela Franceschini, Stefan A Carp","doi":"10.1117/1.NPh.11.1.015004","DOIUrl":"10.1117/1.NPh.11.1.015004","url":null,"abstract":"<p><strong>Significance: </strong>The non-invasive measurement of cerebral blood flow based on diffuse optical techniques has seen increased interest as a research tool for cerebral perfusion monitoring in critical care and functional brain imaging. Diffuse correlation spectroscopy (DCS) and speckle contrast optical spectroscopy (SCOS) are two such techniques that measure complementary aspects of the fluctuating intensity signal, with DCS quantifying the temporal fluctuations of the signal and SCOS quantifying the spatial blurring of a speckle pattern. With the increasing interest in the use of these techniques, a thorough comparison would inform new adopters of the benefits of each technique.</p><p><strong>Aim: </strong>We systematically evaluate the performance of DCS and SCOS for the measurement of cerebral blood flow.</p><p><strong>Approach: </strong>Monte Carlo simulations of dynamic light scattering in an MRI-derived head model were performed. For both DCS and SCOS, estimates of sensitivity to cerebral blood flow changes, coefficient of variation of the measured blood flow, and the contrast-to-noise ratio of the measurement to the cerebral perfusion signal were calculated. By varying complementary aspects of data collection between the two methods, we investigated the performance benefits of different measurement strategies, including altering the number of modes per optical detector, the integration time/fitting time of the speckle measurement, and the laser source delivery strategy.</p><p><strong>Results: </strong>Through comparison across these metrics with simulated detectors having realistic noise properties, we determine several guiding principles for the optimization of these techniques and report the performance comparison between the two over a range of measurement properties and tissue geometries. We find that SCOS outperforms DCS in terms of contrast-to-noise ratio for the cerebral blood flow signal in the ideal case simulated here but note that SCOS requires careful experimental calibrations to ensure accurate measurements of cerebral blood flow.</p><p><strong>Conclusion: </strong>We provide design principles by which to evaluate the development of DCS and SCOS systems for their use in the measurement of cerebral blood flow.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 1","pages":"015004"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10821780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139572203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FiPhA: an open-source platform for fiber photometry analysis.","authors":"Matthew F Bridge, Leslie R Wilson, Sambit Panda, Korey D Stevanovic, Ayland C Letsinger, Sandra McBride, Jesse D Cushman","doi":"10.1117/1.NPh.11.1.014305","DOIUrl":"10.1117/1.NPh.11.1.014305","url":null,"abstract":"<p><strong>Significance: </strong>Fiber photometry (FP) is a widely used technique in modern behavioral neuroscience, employing genetically encoded fluorescent sensors to monitor neural activity and neurotransmitter release in awake-behaving animals. However, analyzing photometry data can be both laborious and time-consuming.</p><p><strong>Aim: </strong>We propose the fiber photometry analysis (FiPhA) app, which is a general-purpose FP analysis application. The goal is to develop a pipeline suitable for a wide range of photometry approaches, including spectrally resolved, camera-based, and lock-in demodulation.</p><p><strong>Approach: </strong>FiPhA was developed using the R Shiny framework and offers interactive visualization, quality control, and batch processing functionalities in a user-friendly interface.</p><p><strong>Results: </strong>This application simplifies and streamlines the analysis process, thereby reducing labor and time requirements. It offers interactive visualizations, event-triggered average processing, powerful tools for filtering behavioral events, and quality control features.</p><p><strong>Conclusions: </strong>FiPhA is a valuable tool for behavioral neuroscientists working with discrete, event-based FP data. It addresses the challenges associated with analyzing and investigating such data, offering a robust and user-friendly solution without the complexity of having to hand-design custom analysis pipelines. This application thus helps standardize an approach to FP analysis.</p>","PeriodicalId":54335,"journal":{"name":"Neurophotonics","volume":"11 1","pages":"014305"},"PeriodicalIF":5.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10885510/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139974593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}