Antibiotics-Basel最新文献

{"title":"Comparative Analysis of Phenotypic and Genotypic Antibiotic Susceptibility of <i>Pasteurella multocida</i> Isolated from Various Host Species in France and Hungary.","authors":"Krisztina Pintér, Marianna Domán, Enikő Wehmann, Hubert Gantelet, Tibor Magyar","doi":"10.3390/antibiotics14090906","DOIUrl":"10.3390/antibiotics14090906","url":null,"abstract":"<p><p><b>Background/Objectives</b>: <i>Pasteurella multocida</i> is responsible for a wide variety of animal diseases worldwide, causing major economic losses. These infections are usually treated with antibiotics; however, the emergence of multidrug-resistant (MDR) strains is increasingly hindering. Understanding antibiotic resistance in <i>P. multocida</i> is important for effective treatment strategies and public health, as it impacts both animal and human welfare. <b>Methods</b>: In this study, the antibiotic susceptibility of 80 <i>P. multocida</i> isolates was evaluated by phenotypic (disk diffusion and broth microdilution) and genotypic analysis via whole-genome sequencing, with particular attention to the occurrence of MDR strains. The strains were tested against antibiotics from nine antimicrobial classes (penicillins, cephalosporins, aminoglycosides, tetracyclines, macrolides, fluoroquinolones, lincosamides, phenicols, and sulfonamides). Antimicrobial resistance gene (ARG) sequences and single-nucleotide polymorphisms (SNPs) were evaluated in paired reads using the Bacterial and Viral Bioinformatics Resource Center (BV-BRC) and the Comprehensive Antibiotic Resistance Database (CARD) via Resistance Gene Identifier (RGI), respectively. <b>Results</b>: Phenotypic results indicated that cephalosporins and phenicols were the most effective drugs against <i>P. multocida</i>; however, the majority of strains were also susceptible to fluoroquinolones and tetracyclines. In contrast, high resistance rates were observed to sulfamethoxazole and clindamycin. The most prevalent resistance genes were <i>str</i>A, <i>sul</i>2, and <i>tet</i>H, while none of the strains harbored the <i>bla</i>-_TEM or <i>erm</i> (42) genes. <b>Conclusions</b>: Of the two phenotypic methods, MIC values showed a stronger positive correlation with genotypic results, making it a more suitable method for determining antibiotic susceptibility. The phenotypic results for phenicols, tetracyclines, and fluoroquinolones showed a strong correlation with the detected resistance genes. In contrast, resistance to sulfamethoxazole, β-lactams, and macrolides remained genetically unexplained, suggesting the existence of additional resistance mechanisms to be explored.</p>","PeriodicalId":54246,"journal":{"name":"Antibiotics-Basel","volume":"14 9","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145180155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Resistance and Therapy in the Intensive Care Unit.","authors":"Pablo Vidal-Cortés, Borja Suberviola-Cañas, David Andaluz-Ojeda","doi":"10.3390/antibiotics14090904","DOIUrl":"10.3390/antibiotics14090904","url":null,"abstract":"<p><p>Antimicrobial resistance (AMR) has emerged as one of the greatest public health challenges in the last decades [...].</p>","PeriodicalId":54246,"journal":{"name":"Antibiotics-Basel","volume":"14 9","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145180227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Antimicrobial Resistance of <i>Enterobacteriaceae</i> in Wild Birds Across Europe: A Systematic Review.","authors":"Daiana-Ionela Cocoș, Eugenia Dumitrescu, Florin Muselin, Diana Brezovan, János Degi, Oana-Maria Boldura, Romeo T Cristina","doi":"10.3390/antibiotics14090905","DOIUrl":"10.3390/antibiotics14090905","url":null,"abstract":"<p><p>Wild birds are increasingly recognized as reservoirs and vectors of antimicrobial-resistant (AMR) <i>Enterobacteriaceae</i>, but comprehensive assessments across Europe remain limited. AMR represents a growing threat to global health under the One Health framework. <b>Background/Objectives:</b> This review aimed to evaluate the occurrence, diversity, and resistance patterns of <i>Enterobacteriaceae</i> in wild birds across Europe (1969-2025), and to identify ecological and methodological trends. <b>Methods:</b> Following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidelines, we searched PubMed and Web of Science until July 2025. Inclusion criteria targeted studies reporting <i>Enterobacteriaceae</i> isolation and/or AMR in free-living European birds. Data were synthesized thematically by bacterial species, avian order, resistance profile, and country. Risk of bias was assessed based on sampling, reporting, and diagnostic clarity. <b>Results:</b> Eighty studies met the inclusion criteria, covering over 18,000 wild birds from 25 countries. <i>Escherichia coli</i> and <i>Salmonella enterica</i> were most reported, often exhibiting resistance to β-lactams, fluoroquinolones, and tetracyclines. AMR was detected in birds from both urban and natural areas. Study designs varied widely, with inconsistent methods for bacterial identification and susceptibility testing. <b>Conclusions:</b> Wild birds in Europe carry resistant <i>Enterobacteriaceae</i>, including strains with clinically relevant resistance profiles. These findings support their inclusion in One Health AMR surveillance and highlight the need for harmonized protocols, expanded molecular tools, and ecological integration.</p>","PeriodicalId":54246,"journal":{"name":"Antibiotics-Basel","volume":"14 9","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145180285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High Prevalence of Antimicrobial Resistance Genes in Rabbit Farms from Sumy Region, Ukraine.","authors":"Sara Gomes-Gonçalves, Jaqueline T Bento, Ana Machado, Yevheniia Dudnyk, Oksana Shkromada, Halyna Rebenko, Adriano A Bordalo, João R Mesquita","doi":"10.3390/antibiotics14090907","DOIUrl":"10.3390/antibiotics14090907","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Antimicrobial resistance (AMR) poses a significant threat to public health, food security, and sustainable livestock production. Despite increasing concern, AMR remains poorly studied in cuniculture, particularly in regions where rabbit farming is predominantly small-scale and household-based. This study aimed to assess the prevalence and diversity of antimicrobial resistance genes (ARGs) in rabbit farms in northeastern Ukraine. <b>Methods</b>: A total of 100 fecal samples were collected from clinically healthy rabbits housed in two farms and one vivarium. DNA was extracted and analyzed using real-time PCR targeting 21 ARGs associated with resistance to major antibiotic classes, including tetracyclines, sulfonamides, β-lactams, macrolides, quinolones, carbapenems, and multidrug efflux systems. <b>Results</b>: A diverse and widespread resistome was identified. The most prevalent genes included sul1 (96%), <i>blaTEM</i> (95%), <i>tetM</i> (94%), and <i>ermB</i> (93%). <i>AcrB</i> (78%) and <i>qnrS</i>/<i>oqxB</i> (approximately 67%) were also frequently detected. Carbapenemase genes (<i>blaKPC</i>, <i>blaNDM</i>, <i>blaVIM</i>) were identified in 6% of samples, despite limited veterinary use of carbapenems. Notably, 96% of samples harbored ARGs from three or more antibiotic classes, indicating a high potential for multidrug resistance. <b>Conclusions</b>: The findings demonstrate a high prevalence and diversity of ARGs in rabbit farming systems in northeastern Ukraine. The presence of clinically significant resistance genes, including those conferring carbapenem resistance, underscores the urgent need for targeted AMR surveillance and improved antibiotic stewardship in cuniculture, particularly in regions with minimal regulatory oversight of antimicrobial use.</p>","PeriodicalId":54246,"journal":{"name":"Antibiotics-Basel","volume":"14 9","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145180309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification and Characterization of Enterocins A, B, and a Novel High-Mass Bacteriocin from <i>Enterococcus lactis</i>-67 with Antilisterial Activity.","authors":"Ezequiel Hernandez-Mendoza, Miguel Ángel Martínez-Téllez, Humberto González-Ríos, Emmanuel Aispuro-Hernández, María de la Cruz Paredes-Aguilar, Alexa Rubí-Soberanes, Etna Aida Peña-Ramos","doi":"10.3390/antibiotics14090903","DOIUrl":"10.3390/antibiotics14090903","url":null,"abstract":"<p><p><b>Background/Objectives:</b><i>Listeria monocytogenes</i> is a high-risk pathogen in the food industry involved in several outbreaks. Bacteriocins are natural-origin antimicrobial peptides or proteins that represent a good alternative to synthetic antimicrobials capable of inhibiting the growth of pathogens. This study aimed to purify and identify bacteriocins from the cell-free supernatant of <i>Enterococcus lactis</i>-67, which exhibits antagonistic activity against <i>L. monocytogenes</i>. <b>Methods:</b> Protein purification was performed by precipitation with ammonium sulfate, dialysis, and fast protein liquid chromatography. Active protein fractions were analyzed by SDS-PAGE and identified by mass spectrometry. <b>Results:</b> In addition to enterocins A and B, a novel 47 kDa bacteriocin with LysM and NlpC/P60 domains, on the N- and C-terminal regions, respectively, was identified. This enterocin has not been described for <i>Enterococcus</i> before. <b>Conclusions:</b> This study contributes to the identification of new natural and effective strategies for ensuring food safety.</p>","PeriodicalId":54246,"journal":{"name":"Antibiotics-Basel","volume":"14 9","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145180357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined Toxicity of Microplastics and Antimicrobials on Animals: A Review.","authors":"Kuok Ho Daniel Tang","doi":"10.3390/antibiotics14090896","DOIUrl":"10.3390/antibiotics14090896","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Microplastics are ubiquitous pollutants that pose physical toxicity and serve as vectors for antimicrobial agents, altering their bioavailability and toxicity. Unlike previous reviews that focus solely on antibiotics and terrestrial or aquatic ecosystems, this review integrates recent findings on the combined impacts of microplastics and antimicrobials on both aquatic and terrestrial animals, highlighting their biological responses. <b>Methods</b>: Recent experimental studies involving aquatic and terrestrial animals published in peer-reviewed journals were reviewed. These studies employed co-exposure designs using microplastics of different sizes, aging conditions, and surface chemistries in combination with antimicrobial compounds. <b>Results</b>: Microplastics combined with antimicrobials cause species-specific and often synergistic toxicity in aquatic organisms, affecting reproduction, immunity, oxidative stress, gene expression, and microbiota, with co-exposure often amplifying adverse physiological and developmental effects. Similarly, co-exposure to microplastics and antimicrobials in rodents, amphibians, birds, and soil invertebrates frequently leads to synergistic toxicity, oxidative stress, disrupted gut microbiota, and enhanced accumulation and bioavailability of pollutants, promoting inflammation, neurotoxicity, metabolic dysfunction, and increased antibiotic resistance gene propagation. Particle size, aging, and antimicrobial type influence toxicity severity. Certain microplastic-antimicrobial combinations can exhibit antagonistic effects, though less frequently reported. <b>Conclusions</b>: The interactions between microplastics and antimicrobials pose heightened risks to the health of organisms and ecological stability. These findings underscore the need to revise current risk assessment protocols to consider pollutant mixtures and microplastics-mediated transport. Future research should focus on environmentally relevant exposures, mechanistic studies using omics tools, and long-term ecological impacts. Integrated regulatory strategies are essential to address the compounded effects of microplastics and chemical contaminants.</p>","PeriodicalId":54246,"journal":{"name":"Antibiotics-Basel","volume":"14 9","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145180061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Resistance Profiles of Clinically Relevant Enterobacteriaceae Isolates: A Retrospective Study at a Southern Italian Hospital.","authors":"Giorgia Stornelli, Beatrice Marinacci, Valentina Puca, Benedetta Pellegrini, Roberta Zita Marulli, Ivana Cataldo, Francesca Sisto, Vittoria Perrotti, Arianna Pompilio, Mattia Mori, Pamela Di Giovanni, Rossella Grande","doi":"10.3390/antibiotics14090899","DOIUrl":"10.3390/antibiotics14090899","url":null,"abstract":"<p><strong>Background: </strong>The Enterobacteriaceae family is the most heterogeneous group of Gram-negative bacilli, with both environmental and clinical relevance. Although many of these species are part of the normal intestinal microbiota, species such as <i>Escherichia coli</i>, <i>Klebsiella pneumoniae</i>, and <i>Proteus mirabilis</i> are among the most common opportunistic pathogens, frequently responsible for nosocomial infections, including urinary tract infections, bacteraemia, and pneumonia. Based on these concerns, these species are increasingly being studied for their ability to develop antimicrobial resistance, often mediated by extended spectrum β-lactamase or carbapenemase production. The present study aims to analyse the antimicrobial resistance profiles of Enterobacteriaceae isolated from a southern Italian hospital focusing on the species of major clinical importance.</p><p><strong>Methods: </strong>A retrospective analysis was carried out on biological samples collected between 2019 and 2023 at the Microbiology Laboratory of the San Pio Hospital, Vasto (Italy). Eight hundred ninety patients were included, with an average age of 73 years. Bacterial identification was carried out using bacterial culture and biochemical methods, while antimicrobial sensitivity was assessed by using the automated Walk Away System.</p><p><strong>Results: </strong>The most frequently isolated species were <i>Escherichia coli</i> (63.2%), <i>Klebsiella</i> spp. (21.9%), and <i>Proteus</i> spp. (8.8%). All isolates showed resistance to at least one antimicrobial and most to more than four. The highest resistance rates were observed for Cefotaxime (16.0%), followed by Ampicillin (15.6%) and Ciprofloxacin (13.2%).</p><p><strong>Conclusions: </strong>The high prevalence of antimicrobial resistance among clinically relevant Enterobacteriaceae species is a growing clinical challenge. The results of this study, which describe the trend of resistance among Enterobacteriaceae in a local hospital, could help to raise awareness highlighting the urgent need for more advanced diagnostic tools and new therapeutic strategies to treat infections caused by multi-resistant microorganisms.</p>","PeriodicalId":54246,"journal":{"name":"Antibiotics-Basel","volume":"14 9","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145180353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological Trends of Carbapenemase-Producing <i>Pseudomonas aeruginosa</i> in a Tertiary Care Hospital in Athens, Greece, During 2020-2023.","authors":"Vasiliki Koumaki, Eleni Voudanta, Aikaterini Michelaki, Maria Orfanidou, Eleni Vagiakou, Georgia Vrioni, Athanasios Tsakris","doi":"10.3390/antibiotics14090898","DOIUrl":"10.3390/antibiotics14090898","url":null,"abstract":"<p><p><b>Background:</b> Infections caused by carbapenemase-producing <i>Pseudomonas aeruginosa</i> (CPPA) isolates have become a worldwide clinical challenge for clinicians due to the limited treatment options. This study provides epidemiological data on CPPA clinical isolates recovered from one of the largest tertiary care hospitals in Athens, Greece, serving a diverse patient population during and after the COVID-19 pandemic. <b>Materials and Methods</b>: The study included all consecutive single-patient CPPA clinical isolates identified from January 2020 to December 2023 in the clinical laboratory. Identification and antimicrobial susceptibility testing were performed using the VITEK-2 automated system. A lateral flow immunoassay and the FilmArray system for blood cultures only were used for the detection of the five most prevalent carbapenemases. Their epidemiological and antimicrobial susceptibility trends were retrospectively analyzed. <b>Results:</b> During the study period, a total of 628 single-patient CPPA were identified among 902 carbapenem-resistant <i>P. aeruginosa</i> clinical isolates. An increasing number of CPPA was revealed during the survey, especially in the post-COVID period (rising from <i>n</i> = 102 in 2020, <i>n</i> = 105 in 2021, and <i>n</i> = 123 in 2022 to <i>n</i> = 298 in 2023; <i>p</i> < 0.05). Regarding the type of carbapenemase, VIM metallo-beta-lactamase was the only carbapenemase identified during the first two years of the study (2020 to 2021). In 2022, VIM- and NDM-producing isolates split almost evenly at proportions of 57% and 43%, respectively. In 2023, NDM-producing isolates seem to have surpassed VIM producers with rates of 54.4% and 45.6%, respectively. As far as antimicrobial resistance profiles, high rates of resistance were observed for most of the antipseudomonal drugs, exceeding 90% across all study years, with little significant variation. However, aztreonam exhibited moderate activity and colistin exhibited excellent activity, remaining the most viable drugs in this setting. <b>Conclusions:</b> Following the COVID-19 pandemic, an increase in CPPA pathogens was identified, while an epidemiological shift was also observed, with the carbapenemase NDM dominating over VIM since 2023. Continuous surveillance is required to track resistance patterns and guide empirical therapy. In this context, new antimicrobials and antimicrobial combinations are also urgently needed.</p>","PeriodicalId":54246,"journal":{"name":"Antibiotics-Basel","volume":"14 9","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145180095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Cost Savings of Methods of Prevention of Orthopedic Implant-Associated Infection in Arthroplasty and Closed Fracture Surgery Patients.","authors":"Pedro Augusto Maciel Moreira, Thiago de Carvalho Gontijo, Gabriel Costa Colen, Ana Carolina Morganti, Felipe Ismael Ulloa Gómez, Pedro Assis Mourão, Gabrielle Adriane Rodrigues Mota, Braulio R G M Couto, Patrícia Almeida de Vasconcellos Rocha, Laila Gonçalves Machado, Raquel Bandeira da Silva, Mauro José Salles","doi":"10.3390/antibiotics14090900","DOIUrl":"10.3390/antibiotics14090900","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Surgical site infections (SSIs) are serious complications in orthopedic implant-associated procedures, increasing morbidity, mortality, and hospital costs. The purpose of this study was to evaluate the impact of a structured infection prevention and control (IPC) service on SSI incidence and cost savings across hip arthroplasty (HA), knee arthroplasty (KA), and open reduction and internal fixation (ORIF). <b>Methods</b>: A retrospective analysis included 6930 patients treated between 2019 and 2024, divided into pre-intervention (2019-2022) and post-intervention (2023-2024) cohorts. Preventive methods (PMs) comprised enhanced antibiotic prophylaxis, Staphylococcus aureus screening, chlorhexidine bathing, intraoperative audits, and behavioral interventions. Economic evaluation used literature-based costs, standardized to 2024 US dollars (USD 2024), with sensitivity analyses performed. <b>Results</b>: SSI incidence decreased from 5.6% to 1.1% overall (<i>p</i> < 0.001), with consistent reductions across procedures: ORIF (5.2%→1.0%), HA (9.2%→2.4%), and KA (4.8%→1.2%). In 2023, approximately 31 SSIs and one infection-related death were prevented, avoiding 308 hospital days. Cost savings ranged from USD 209,188 to USD 376,898, with cost saving confirmed in 93% of simulations. <b>Conclusions</b>: Comprehensive infection-prevention protocols, delivered through a structured IPC service, significantly reduced SSIs and generated substantial cost savings. These findings support wider use of these PMs in orthopedic surgery.</p>","PeriodicalId":54246,"journal":{"name":"Antibiotics-Basel","volume":"14 9","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145180118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotransformation of Antibiotics by <i>Coriolopsis gallica</i>: Degradation of Compounds Does Not Always Eliminate Their Toxicity.","authors":"Bouthaina Ghariani, Héla Zouari-Mechichi, Abdulrahman H Alessa, Hussain Alqahtani, Ahmad A Alsaigh, Tahar Mechichi","doi":"10.3390/antibiotics14090897","DOIUrl":"10.3390/antibiotics14090897","url":null,"abstract":"<p><strong>Background/objectives: </strong>Wastewaters containing antibiotics pose risks to human health and soil ecosystems. In this study, the white-rot fungus <i>Coriolopsis gallica</i> (a basidiomycete exhibiting high laccase production) was used for the biotransformation of three antibiotics (50 mg L^-1): tetracycline, chloramphenicol, and sulfanilamide.</p><p><strong>Methods: </strong>The biotransformation process was investigated in liquid and solid media using high-performance liquid chromatography (HPLC) and the bacterial growth inhibition agar well diffusion method, respectively.</p><p><strong>Results: </strong>Among the three antibiotics tested, tetracycline showed the highest biotransformation efficiency, achieving a 100% removal rate in the liquid medium and a 100% decrease in the growth inhibition of <i>Escherichia coli</i> in the solid medium. Chloramphenicol and sulfanilamide were partially removed (20% and 16%, respectively) after 12 days of treatment in more than one step without the loss of their antibacterial activities. The presence of these antibiotics in the culture medium of <i>C. gallica</i> enhanced laccase activity, indicating that this ligninolytic enzyme might participate in the biotransformation process.</p><p><strong>Conclusions: </strong>Thus, the results reported in this article extend our knowledge of the catalytic potential of <i>C. gallica</i> and give further perspectives for its application in the biodegradation of antibiotics. To the best of our knowledge, this is the first study wherein <i>C. gallica</i> was used for the treatment of tetracycline, chloramphenicol, and sulfanilamide.</p>","PeriodicalId":54246,"journal":{"name":"Antibiotics-Basel","volume":"14 9","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145180008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}