Purification and Characterization of Enterocins A, B, and a Novel High-Mass Bacteriocin from Enterococcus lactis-67 with Antilisterial Activity.

IF 4.6 2区医学 Q1 INFECTIOUS DISEASES

Antibiotics-Basel Pub Date : 2025-09-06 DOI:10.3390/antibiotics14090903

Ezequiel Hernandez-Mendoza, Miguel Ángel Martínez-Téllez, Humberto González-Ríos, Emmanuel Aispuro-Hernández, María de la Cruz Paredes-Aguilar, Alexa Rubí-Soberanes, Etna Aida Peña-Ramos

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引用次数: 0

Abstract

Background/Objectives:Listeria monocytogenes is a high-risk pathogen in the food industry involved in several outbreaks. Bacteriocins are natural-origin antimicrobial peptides or proteins that represent a good alternative to synthetic antimicrobials capable of inhibiting the growth of pathogens. This study aimed to purify and identify bacteriocins from the cell-free supernatant of Enterococcus lactis-67, which exhibits antagonistic activity against L. monocytogenes. Methods: Protein purification was performed by precipitation with ammonium sulfate, dialysis, and fast protein liquid chromatography. Active protein fractions were analyzed by SDS-PAGE and identified by mass spectrometry. Results: In addition to enterocins A and B, a novel 47 kDa bacteriocin with LysM and NlpC/P60 domains, on the N- and C-terminal regions, respectively, was identified. This enterocin has not been described for Enterococcus before. Conclusions: This study contributes to the identification of new natural and effective strategies for ensuring food safety.

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乳酸肠球菌-67中具有抗李斯特菌活性的肠球菌素A、B和新型高质量细菌素的纯化和特性

背景/目的：单核细胞增生李斯特菌是食品工业中一种高风险病原体，曾多次暴发。细菌素是天然来源的抗菌肽或蛋白质，代表了能够抑制病原体生长的合成抗菌药物的良好替代品。本研究旨在从乳酸肠球菌-67的无细胞上清液中纯化和鉴定对单核增生乳杆菌具有拮抗活性的细菌素。方法：采用硫酸铵沉淀、透析、快速蛋白液相色谱法纯化蛋白质。活性蛋白组分经SDS-PAGE分析，质谱鉴定。结果：除了肠毒素A和肠毒素B外，还鉴定出一种新的47 kDa的细菌素，分别在N端和c端具有LysM和NlpC/P60结构域。这种肠球菌蛋白以前没有被描述过。结论：本研究有助于确定新的天然有效的食品安全保障策略。

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来源期刊

Antibiotics-Basel Pharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics

CiteScore

7.30

自引率

14.60%

发文量

1547

审稿时长

11 weeks

期刊介绍： Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.