Problems of Cryobiology and Cryomedicine最新文献_第9页

Dynamics of Dimethyl Sulfoxide Penetration Into L929 Cells and L929-Based Spheroids 二甲基亚砜对L929细胞和L929基球体的渗透动力学

Problems of Cryobiology and Cryomedicine Pub Date : 2021-12-25 DOI: 10.15407/cryo31.04.316

A. Moisieiev, I. Kovalenko, S.Ju. Kovalenko, G. Bozhok, O. Gordienko

引用次数: 2

Anti-Inflammatory Properties of Diclofenac Sodium on Background of Its Combined Use With Cryopreserved Placenta Extract in Experiment 双氯芬酸钠与胎盘冻存液联合应用的抗炎作用研究

Problems of Cryobiology and Cryomedicine Pub Date : 2021-12-25 DOI: 10.15407/cryo31.04.364

F. Hladkykh

引用次数: 7

Placental Cryoextract Corrects the Level of Thyroid Hormones in Rats 胎盘冷冻提取物对大鼠甲状腺激素水平的影响

Problems of Cryobiology and Cryomedicine Pub Date : 2021-12-25 DOI: 10.15407/cryo31.04.353

N. Malova, I. Komarova, L. Sirotenko, N. Brechka, Yuliya Kurilko, T. Komisova, A. Goltsev

{"title":"Placental Cryoextract Corrects the Level of Thyroid Hormones in Rats","authors":"N. Malova, I. Komarova, L. Sirotenko, N. Brechka, Yuliya Kurilko, T. Komisova, A. Goltsev","doi":"10.15407/cryo31.04.353","DOIUrl":"https://doi.org/10.15407/cryo31.04.353","url":null,"abstract":"Effectiveness of cryopreserved placental drug ‘Placental cryoextract’ to correct the reproductive system disorders, occurring against the background of low levels of thyroid hormones, was studied in 50 male rats with mercazolyl hypothyroidism. Using standard test systems for animals, the level of total and free forms of thyroid hormones, total testosterone and estradiol was determined. The morphofunctional state of sperm (number, motility, pathological forms) was traditionally assessed. It was shown that the introduction of the biological product (at a dose of 50–150 μl / kg) restored the functional activity of the thyroid gland of animals, and the lowest dose was more effective. Placental cryoextract also had a dose-dependent effect on the reproductive system of animals with hypothyroidism: the most effective dose was 150 μl / kg, which maximally restored the morphofunctional parameters of sperm and stimulated testosterone production as well as normalized the Ts / E2 ratio. Cryopreserved placenta extract can be considered as a potential drug for the correction of hypothyroidism and reproductive disorders of endocrine origin in male rats.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45997835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cultural Properties of Cryopreserved Thymic Multipotent Stromal Cells and Fetal Skin and Muscle-Derived Cells 胸腺多能基质细胞及胎儿皮肤和肌肉来源细胞的培养特性

Problems of Cryobiology and Cryomedicine Pub Date : 2021-09-25 DOI: 10.15407/cryo31.03.249

Valentina Nikolska, Y. Semenova, Lyuba Taranukha, Ihor Nikolsky

{"title":"Cultural Properties of Cryopreserved Thymic Multipotent Stromal Cells and Fetal Skin and Muscle-Derived Cells","authors":"Valentina Nikolska, Y. Semenova, Lyuba Taranukha, Ihor Nikolsky","doi":"10.15407/cryo31.03.249","DOIUrl":"https://doi.org/10.15407/cryo31.03.249","url":null,"abstract":"The paper provides a comparison of properties of cryopreserved fetal murine multipotent stromal cells (MSCs) of skin-muscular origin and those derived from adult thymus in culture in vitro. Fetal MSCs showed a 30% higher number of average population doublings within 24 hrs, and 41% lower average population doubling time. It was found that the fetal MSCs of the 4th passage had a 39% higher clonogenic activity than the adult thymus-derived ones. Fetal MSCs and those derived from adult thymus differentiated in osteogenic and adipogenic lineages with equal efficiency in special culture media. Fetal and thymus-derived MSCs were characterized by almost the same high ability of contact interaction with thymocytes, and the fibroblast-lymphocyte rosette (FLR) formation. They were far less active in FLR formation with lymph node cells. This indicated the presence of membrane affinity for immature lymphoid cells in both MSC subpopulations. The results showed the fetal MSCs to be significantly different from the adult thymus-derived MSCs by more active kinetics of growth and clonogenic potential. However, both cell subpopulations had virtually the same ability for linear differentiation and showed high activity during contact with immature lymphoid cells. Linear differentiation and the ability to interact with lymphocytes were found to be quite stable properties of MSCs, but a proliferative activity and in vitro colony formation distinguished significantly in different types of MSCs. This can be taken into account when choosing the cells for therapy, research and results assessment.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42361435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Theoretical Estimation of Optimal Linear Cooling Rate for PK-15 Cell Suspension PK-15细胞悬浮液最佳线性冷却速率的理论估计

Problems of Cryobiology and Cryomedicine Pub Date : 2021-09-25 DOI: 10.15407/cryo31.03.214

O. Gordienko, I. Kovalenko, S.Ju. Kovalenko, L. Kuleshova, O. Todrin

{"title":"Theoretical Estimation of Optimal Linear Cooling Rate for PK-15 Cell Suspension","authors":"O. Gordienko, I. Kovalenko, S.Ju. Kovalenko, L. Kuleshova, O. Todrin","doi":"10.15407/cryo31.03.214","DOIUrl":"https://doi.org/10.15407/cryo31.03.214","url":null,"abstract":"Preservation of cells during crystallization of the cell suspension is influenced by two types of damaging factors. The first type of cryoinjury occurs during the crystallization of the extracellular environment and is caused by dehydration of cells, increasing the concentration and ionic strength of extracellular and intracellular solutions. As the cooling rate rises, the damage rate of the first type decreases as a result of the reduced time of action of damaging factors. The second type of cryoinjury is intracellular crystallization, the probability of which enhances at high cooling rates, is considered the most destructive to cells. The optimal linear cooling rate for PK-15 cells is determined using a physico-mathematical model, which describes the probability of cryoinjury of cells in the linear freezing mode and is based on the two-factor theory of cryoinjury, thermodynamic theory of homogeneous crystallization and general theory of activation-type processes. The findings have shown that within the range of cooling rates < 0.5 °C/min the cryoinjury of PK-15 cells occurs mainly due to the effects of the solution, and at cooling rates > 2.5 °C/min this was mainly resulted from an intracellular crystallization. The dependence of the percentage of damaged cells on the cooling rate has a relatively wide minimum within the range of cooling rates of 0.5 °C/min… 2.5 °C/min.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46539074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Impact of Dorsal Root Ganglia Cryoextract on Histological Steatures in Dices and Contractility of Uterus in Differently Aged Rats 背根神经节冷冻提取物对不同年龄大鼠骰子组织学稳定性和子宫收缩能力的影响

Problems of Cryobiology and Cryomedicine Pub Date : 2021-09-25 DOI: 10.15407/cryo31.03.258

H. Nesteruk, V. Ustichenko, N. Alabedalkarim, V. Padalko, O. Protsenko, Ievgen Legach

{"title":"Impact of Dorsal Root Ganglia Cryoextract on Histological Steatures in Dices and Contractility of Uterus in Differently Aged Rats","authors":"H. Nesteruk, V. Ustichenko, N. Alabedalkarim, V. Padalko, O. Protsenko, Ievgen Legach","doi":"10.15407/cryo31.03.258","DOIUrl":"https://doi.org/10.15407/cryo31.03.258","url":null,"abstract":"To date, the number of women giving birth to their first child in late reproductive age is increasing around the world. This stipulates a need in designing the new approaches to restore the uterine contractile activity. In this paper, the histological features of uterus and its contractile activity have been experimentally studied in differently aged rats after the dorsal root ganglia cryoextract (DRGCE) administration. The cryoextract was derived from dorsal root ganglia of neonatal piglets by three-fold freezing down to –196°C in saline, followed by thawing at room temperature, homogenization and centrifugation. Here, we used the female rats of reproductive age (RA, 6-month-old) and those of late reproductive age (LRA, 14-month-old). Animals of both age groups received intraperitoneally either DRGCE (experimental groups) or saline (control groups) for 9 days by 0.2 ml. To days 28–29 after administration beginning the animals were sacrificed and the uterine fragments were taken for histological examination and study of oxytocin (OT)-induced uterine contractile activity (UCA). The strength of OT-induced uterine isometric contraction was found to decrease by 28.6% in LRA rats vs. the RA ones. The DRGCE administration to LRA rats increased the UCA indices, namely the contractile frequency, contractile amplitude and strength of isometric contraction augmented by 25, 9.8 and 30% respectively, as compared with the group of the same age without DRGCE introduction. This effect was observed on the background normal uterine histological structure and unchanged myometrial thickness.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44497752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Influence of Ways to Thaw Bull Sperm on Its Quality 牛精子解冻方式对其品质的影响

Problems of Cryobiology and Cryomedicine Pub Date : 2021-09-25 DOI: 10.15407/cryo31.03.277

Yu. A. Shakhova, A. Paliy, A. Paliy, O. Shkromada, Y. Musiienko, I. Bondarenko

引用次数: 2

Role of Liquid-Liquid Phase Transitions in Mechanism of Erythrocyte Protection During Cooling with CRIHBT-115 Cryopreservative Agent 液-液相变在CRIHBT-115抗冻剂冷却过程中红细胞保护机制中的作用

Problems of Cryobiology and Cryomedicine Pub Date : 2021-09-25 DOI: 10.15407/cryo31.03.236

O. Khodko

引用次数: 0

Manufacturing Characteristics of Probiotic Esherichia Coli Strain M-17 After Immobilization in Gel Carriers and Storage at Various Low Temperatures 大肠埃希氏菌M-17凝胶载体固定化及不同低温贮藏后的生产特性

Problems of Cryobiology and Cryomedicine Pub Date : 2021-09-25 DOI: 10.15407/cryo31.03.273

I. Petrov, I. Vysekantsev, Y. Cherkashina, O. Peretyatko, Yu. Yagniuk

{"title":"Manufacturing Characteristics of Probiotic Esherichia Coli Strain M-17 After Immobilization in Gel Carriers and Storage at Various Low Temperatures","authors":"I. Petrov, I. Vysekantsev, Y. Cherkashina, O. Peretyatko, Yu. Yagniuk","doi":"10.15407/cryo31.03.273","DOIUrl":"https://doi.org/10.15407/cryo31.03.273","url":null,"abstract":"Іммобілізовані пробіотики все більше застосовують у виробництві пробіотичних препаратів, лікувально-профілактичних продуктів харчування, кормових домішок [6]. Технології довгострокового зберігання комерційних препаратів і продуктів на основі іммобілізованих пробіотиків знаходяться в стадії розробки. Найбільш ефективними і широко вживаними методами консервування вільних клітин різних мікроорганізмів є ліофілізація і зберігання за низьких температур [5]. Мета роботи – вивчення збереженості виробничих характеристик пробіотичного штаму Esherichia coli M-17 (E. coli M-17) після іммобілізації в гелевих носіях і зберігання за різних низьких температур.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42122922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermoplastic Analysis of Cluster Crystallization of Cryoprotective Solutions 低温保护溶液团簇结晶的热塑性分析

Problems of Cryobiology and Cryomedicine Pub Date : 2021-09-25 DOI: 10.15407/cryo31.03.203

Oleksandr Osetskyi, T. Gurina, Anna Poliakova, Stanislav Sevastianov

引用次数: 1