Thermoplastic Analysis of Cluster Crystallization of Cryoprotective Solutions

Q4 Medicine

Problems of Cryobiology and Cryomedicine Pub Date : 2021-09-25 DOI:10.15407/cryo31.03.203

Oleksandr Osetskyi, T. Gurina, Anna Poliakova, Stanislav Sevastianov

引用次数: 1

Abstract

For the first time the possibility of using thermomechanical analysis to construct the fragments of state diagrams of cryoprotective solutions in the zone of glass transition temperatures has been considered. A method for studying cluster crystallization of cryoprotective solutions based on thermomechanical curves has been developed. The parameters of thermomechanical curves of frozen aqueous solutions of dimethyl sulfoxide (DMSO), polyethylene oxide 1500 (PEO-1500), glycerol were analyzed and the relationship between these parameters and the cluster crystallization kinetics for these solutions was established. On the basis of experimentally obtained thermomechanical curves for the frozen solutions of DMSO and PEO-1500 the possibility of formation of clusters of two types has been shown: on the basis of ice and cryoprotective substance microcrystals. Additional experimental data were obtained to construct a complete state diagrams of cryoprotective solutions, which include the existing cluster phase areas.

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低温保护溶液团簇结晶的热塑性分析

首次考虑了在玻璃化转变温度区使用热机械分析构建冷冻保护溶液状态图片段的可能性。开发了一种基于热机械曲线研究低温保护溶液簇结晶的方法。分析了二甲基亚砜（DMSO）、聚环氧乙烷1500（PEO-1500）、甘油冷冻水溶液的热力学曲线参数，建立了这些参数与溶液团簇结晶动力学的关系。根据实验获得的DMSO和PEO-1500冷冻溶液的热机械曲线，已经表明了形成两种类型团簇的可能性：基于冰和冷冻保护物质微晶。获得了额外的实验数据，以构建冷冻保护溶液的完整状态图，其中包括现有的簇相区域。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Problems of Cryobiology and Cryomedicine Medicine-Medicine (miscellaneous)

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine. The journal covers all topics related to low temperature biology, medicine and engineering. These include but are not limited to: low temperature storage of biologicals (human, animal or plant cells, tissues, and organs), including preparation for storage, thawing/warming, cell and tissue culturing etc. response of biologicals to low temperature; cold adaptation of animals and plants; utilisation of low temperature in medicine; experimental and clinical transplantation, cell and tissue based therapies; developing of cryobiological and cryomedical devices; organisation and functioning of low temperature banks etc.