Problems of Cryobiology and Cryomedicine最新文献

Brain Cortex Morphology in Rats After Cold Exposures 寒冷暴露后大鼠的大脑皮层形态学

Problems of Cryobiology and Cryomedicine Pub Date : 2023-09-30 DOI: 10.15407/cryo33.03.193

V. Lomako, O. Shylo, L. Pirozhenko

引用次数: 0

Therapeutic Hypothermia and Cell Therapy Change Cognitive Functions of Spontaneously Hypertensive Rats 治疗性低温和细胞疗法改变自发性高血压大鼠的认知功能

Problems of Cryobiology and Cryomedicine Pub Date : 2023-09-30 DOI: 10.15407/cryo33.03.201

O. Kudokotseva, I. Lomakin, V. Babiichuk, L. Babiichuk, Igor Kandybko

{"title":"Therapeutic Hypothermia and Cell Therapy Change Cognitive Functions of Spontaneously Hypertensive Rats","authors":"O. Kudokotseva, I. Lomakin, V. Babiichuk, L. Babiichuk, Igor Kandybko","doi":"10.15407/cryo33.03.201","DOIUrl":"https://doi.org/10.15407/cryo33.03.201","url":null,"abstract":"The paper presents the research results of spatial memory and learning processes for the comprehensive analysis of cognitive function of spontaneously hypertensive rats under the influence of independent and combined use of rhythmic craniocerebral hypothermia (RCH) and the introduction of cryopreserved cord blood nucleated cells (cCBNCs). It was shown that SHR rats have a cognitive deficit, which is manifested in reduced speed of the learning process and memory formation in the Morris water maze (MWM). The administration of therapeutic RCH and/or cell therapy (cCBNCs introduction) 30 days before training in the MWM improved the cognitive functions of SHR rats: the target search strategy changed, and the time to search for a platform hidden under water was significantly reduced. These changes indicated the acceleration of the processes of information perception, learning, and the formation of spatial memory. The preservation and reproduction of long-term memory and as a result of the formed strategy of direct finding of the target in the MWM was most influenced by certain therapy with the samples of cryopreserved cord blood in combination with the procedure of therapeutic hypothermia. It was established that this approach exerted a neuroprotective effect, contributing to the partial elimination of cognitive deficits in SHR by improving spatial memory and accelerating learning processes.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139332674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quarter-Century Experience in Cryopreservation of Human Oocytes by Vitrifi cation. What Has Been Achieved and What is Next? 通过玻璃化冷冻保存人类卵母细胞的四分之一世纪经验。已取得的成就和下一步计划？

Problems of Cryobiology and Cryomedicine Pub Date : 2023-09-30 DOI: 10.15407/cryo33.03.177

T. Yurchuk, M. Petrushko

引用次数: 0

Can Cold-Tolerant Hybrids Be Created by Crossing Cold-Tolerant Parental Lines? 耐寒亲本杂交能否培育出耐寒杂交种？

Problems of Cryobiology and Cryomedicine Pub Date : 2023-09-30 DOI: 10.15407/cryo33.03.212

V. Kolomatska, Liana Liana, Valentyna Syvenko, Nina Leonova

引用次数: 0

Cryobiology and Cryomedicine Techniques’ Boosting in Wartime 战时低温生物学和低温医学技术的发展

Problems of Cryobiology and Cryomedicine Pub Date : 2023-06-30 DOI: 10.15407/cryo33.02.077

Anatoliy Goltsev, Mykola Bondarovych, Nataliia Volkova, Olena Lutsenko, Yuliia Gaevska, Natalia Babenko, Olha Chelombytko, Maksym Ostankov, Dmytro Vvedenskyi, Hanna Kisielova, Tetiana Dubrava

引用次数: 0

Protective Media Composition and Freezing Regimen Experimental Substantiation During Mycobacterium Avium Freeze-Drying 禽分枝杆菌冻干过程中保护介质组成及冷冻方案的实验验证

Problems of Cryobiology and Cryomedicine Pub Date : 2023-06-30 DOI: 10.15407/cryo33.02.139

Andrii Zavhorodnii, Viktor Bilushko, Mykola Kalashnyk, Svitlana Pozmogova, Anatoliy Paliy, Olena Pavlichenko

引用次数: 0

Heat Shock Proteins as Key Triggers to Form Cryopreserved Progenitors-Derived Tolerogenic Dendritic Cells 热休克蛋白是形成低温保存的祖细胞衍生耐受性树突状细胞的关键触发因素

Problems of Cryobiology and Cryomedicine Pub Date : 2023-06-30 DOI: 10.15407/cryo33.02.144

Hanna Kisielova, Tetiana Dubrava, Anatoliy Goltsev

引用次数: 0

Trolox Antioxidant as a Factor in Stabilization of Human Cord Blood Nucleated Cells During Cryopreservation Trolox抗氧化剂在人脐带血有核细胞冷冻保存过程中的稳定作用

Problems of Cryobiology and Cryomedicine Pub Date : 2023-06-30 DOI: 10.15407/cryo33.02.122

Pavlo Zubov, Oksana Zubova, Lyubov Babijchuk

{"title":"Trolox Antioxidant as a Factor in Stabilization of Human Cord Blood Nucleated Cells During Cryopreservation","authors":"Pavlo Zubov, Oksana Zubova, Lyubov Babijchuk","doi":"10.15407/cryo33.02.122","DOIUrl":"https://doi.org/10.15407/cryo33.02.122","url":null,"abstract":"The paper presents experimental data on the determination of preservation rate of human cord blood nucleated cells and their apoptosis/necrosis stages to determine the number of viable functionally active cells after cryopreservation in solutions with different concentrations of DMSO and the water-soluble analogue of vitamin E, the antioxidant trolox. Counting the number of human cord blood nucleated cells after freezing in media with the addition of trolox revealed their maximum preservation in the samples with 7.5% DMSO and 50, 70 or 200 μM of the antioxidant. Using the flow cytometry with the addition of the Annexin V FITC reagent, which specifically binds to phospholipids, and the DNA dye 7-amino-actinomycin D (7-AAD), it was established that trolox in concentrations of 50–70 μM provided an increase in the number of viable cells with intact membrane (AnnexinV─/7AAD─) by 12–16% compared to the control, which involved the use of only DMSO in the cryoprotective solution. The obtained results indicate the effectiveness of using the antioxidant trolox and the prospects of developing trolox-containing cryoprotective mixtures for freezing and long-term storage of nucleated cord blood cells, including hematopoietic progenitor cells.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136366748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiotic Sensitivity of Escherichia Coli Museum Strains After Long-Term Storage in a Lyophilized State at Moderately Low Temperatures 中低温长期冻干状态下大肠杆菌博物馆菌株对抗生素的敏感性

Problems of Cryobiology and Cryomedicine Pub Date : 2023-06-30 DOI: 10.15407/cryo33.02.115

Yulia Yagnuk, Tetiana Gurina, Olena Peretyatko, Nadia Sklyar, Аndrii Yagnuk, Svitlana Kalinichenko

{"title":"Antibiotic Sensitivity of Escherichia Coli Museum Strains After Long-Term Storage in a Lyophilized State at Moderately Low Temperatures","authors":"Yulia Yagnuk, Tetiana Gurina, Olena Peretyatko, Nadia Sklyar, Аndrii Yagnuk, Svitlana Kalinichenko","doi":"10.15407/cryo33.02.115","DOIUrl":"https://doi.org/10.15407/cryo33.02.115","url":null,"abstract":"Sensitivity of microbial cultures to antibiotics was determined using disco-diffusion method, broth serial dilution and agar serial dilution. Restored viability and detection of antibiotic resistance in the museum Escherichia coli strains stored for a lasting time in a lyophilized state indicates that freeze-drying in combination with moderately low temperatures for subsequent long-term storage is an eff ective method of maintaining collection microorganism strains in a functionally stable state, including preservation of their antibiotic resistance. It was found that one of the 21 studied strains was resistant to ampicillin, ampicillin-sulbactam, cephalosporins of the I–III generations, two strains were resistant to chloramphenicol, and four strains were insensitive to tetracycline. Detection of the resistance to cephalosporins and protected penicillins in E. coli strain isolated in 1963, long before the discovery and use of these antibiotics in clinical practice, confirms that antibiotic resistance is a phenomenon associated rather with the general mechanisms of adaptation of pathogens to adverse external environmental factors than with the widespread use of antimicrobial drugs.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136366758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Effectiveness of Umbilical Cord Cryoextract in Aesthetic Medicine (in Vitro Study) 脐带冷冻提取物在美容医学中的效果评价(体外研究)

Problems of Cryobiology and Cryomedicine Pub Date : 2023-06-30 DOI: 10.15407/cryo33.02.133

Nadiia Shevchenko, Olena Terekhova, Anna Kaverinska, Olga Prokopiuk, Viktoriya Lazurenko, Volodymyr Prokopіuk

引用次数: 0