Problems of Cryobiology and Cryomedicine最新文献_第10页

Gel Chromatographic Examination of Serum of Rats and Hamsters Under Artificial and Natural Hibernation 人工和自然冬眠大鼠和仓鼠血清的凝胶色谱检测

Problems of Cryobiology and Cryomedicine Pub Date : 2021-09-25 DOI: 10.15407/cryo31.03.191

O. Shylo, V. Lomako, O. Semenchenko

{"title":"Gel Chromatographic Examination of Serum of Rats and Hamsters Under Artificial and Natural Hibernation","authors":"O. Shylo, V. Lomako, O. Semenchenko","doi":"10.15407/cryo31.03.191","DOIUrl":"https://doi.org/10.15407/cryo31.03.191","url":null,"abstract":"In this study, molecular composition of hamster and rat blood was studied by gel permeation chromatography under natural (NH) and artificial hibernation (AH). The control group was represented by 5 fractions of molecules in hamsters and 7 in rats. The areas under peaks of the fractions similar in molecular weight in hamsters and rats were larger in rats. NH was characterized by appearance of new fractions (1,350, 2,350, and 6,350 Da) and an increase in areas under peaks of the control fractions (1,140 and 1,980 Da). Artificial hibernation in rats led to an increase in areas under peaks of 1,140 Da fraction, a decrease in that of 530 Da, and disappearance of 1290 Da, as well as the appearance of new fractions (650, 830, 950, 2350, and 5110 Da). Two hrs of later AH, the areas under peaks of 1,140 and 1,520 Da fractions were greater and that of 530 Da was lesser; 650, 2,350 and 5,110 Da fractions disappeared, 1,290 Da reappeared and new fraction of 4,030 Da appeared. New fractions of 5,820 and 6,530 Da were found 24 hrs later. In hamsters under AH, the areas under peaks of 1,140, 1,600, and 3,330 Da increased; as well as a new peak in 5,280 Da appeared, both in the control rats and those in 24 hrs after AH.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42075106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Placental Cryoextract and Renin-Angiotensin-Aldosterone System Blockade Mitigate Renal Failure in Rats 胎盘冷冻提取物和肾素-血管紧张素-醛固酮系统阻断减轻大鼠肾功能衰竭

Problems of Cryobiology and Cryomedicine Pub Date : 2021-09-25 DOI: 10.15407/cryo31.03.223

M. Repin, Yu.V. Chyzh, L. Marchenko, Tetyana Govorukha, Oleksandr Brusentsov

引用次数: 1

Influence of Cryopreservation on Phenotype and Functional Properties of Multipotent Mesenchymal Stromal Cells Derived From Different Sources 冷冻保存对不同来源的多能干间充质基质细胞表型和功能特性的影响

Problems of Cryobiology and Cryomedicine Pub Date : 2021-09-25 DOI: 10.15407/cryo31.03.268

N. Volkova, Dmytro Vvedensky, M. Yukhta, Аnatoliy Goltsev

引用次数: 0

Comparative Evaluation of Wound Healing Effect of Cord Blood Low Molecular Weight Fraction (Below 5 kDa) and Actovegin Drug in Skin Cryolesions 脐血低分子量组分（5kDa以下）与Actovegin药物对皮肤冷冻损伤愈合效果的比较评价

Problems of Cryobiology and Cryomedicine Pub Date : 2021-06-30 DOI: 10.15407/cryo31.02.116

O. Gulevsky, N. Moisieieva, O. Gorina, Yu. S. Akhatova

引用次数: 0

Influence of Hypothermia and Cell Therapy on Structural Changes in Brain Tissues of Rats with Dyscirculatory Encephalopathy of Mixed Genesis 低温和细胞治疗对混合型循环障碍性脑病大鼠脑组织结构变化的影响

Problems of Cryobiology and Cryomedicine Pub Date : 2021-06-25 DOI: 10.15407/cryo31.02.151

Vladyslav Babiychuk, V. Aidarova, I. Lomakin, O. Kudokotseva, Roy Nataliia, O. Naumova, О. Protsenko

{"title":"Influence of Hypothermia and Cell Therapy on Structural Changes in Brain Tissues of Rats with Dyscirculatory Encephalopathy of Mixed Genesis","authors":"Vladyslav Babiychuk, V. Aidarova, I. Lomakin, O. Kudokotseva, Roy Nataliia, O. Naumova, О. Protsenko","doi":"10.15407/cryo31.02.151","DOIUrl":"https://doi.org/10.15407/cryo31.02.151","url":null,"abstract":"The study examines the morphological changes that occur in brain of SHR rats on the background of developing hypertensive dyscirculatory encephalopathy (DE), complicated by chronic alcohol intoxication (CAI). CAI has been proven to potentiate the progressive aging of brain in rats with hypertensive DE by aggravating its course and to lead to the development of DE of mixed genesis. The effectiveness of a single and combined use of rhythmic craniocerebral hypothermia (rCCH) and the introduction of cryopreserved cord blood nucleated cells (cCBNCs) to reduce the progressive vascular lesions of brain and the effects of chronic ischemia in animals with DE of mixed genesis. It has been proven that the combined use of rCCH and cCBNCs in the rats with a mixed DE had a more pronounced positive effect on morphological structure of brain tissues and vessels than their single use. There was a further decrease in the signs of dystrophic, destructive and pathologically altered compensatory adaptive processes in brain on the background of a significant decrease in neuroglial index, stimulation of angiogenesis, complete or partial absence of signs of smooth muscle cell hyperplasia, acute hemorrhages and ischemic strokes, spasms of arterial vessels’ walls and plethory in veins, that contributed to restoration of brain microhemocirculation and cytoarchitectonics.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47449579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of Interaction of Glycerol Cryoprotectant and Its Derivatives with Dimethylacetamide in Aqueous Solution Using Fluorescent Probes 用荧光探针研究甘油低温保护剂及其衍生物与二甲基乙酰胺在水溶液中的相互作用

Problems of Cryobiology and Cryomedicine Pub Date : 2021-06-25 DOI: 10.15407/cryo31.02.139

Tetiana Dyubko, V. Pivovarenko, V. Chekanova, Yuliya Pakhomova, Yana Gvozdiuk, A. Kompaniets, A. Tatarets

{"title":"Study of Interaction of Glycerol Cryoprotectant and Its Derivatives with Dimethylacetamide in Aqueous Solution Using Fluorescent Probes","authors":"Tetiana Dyubko, V. Pivovarenko, V. Chekanova, Yuliya Pakhomova, Yana Gvozdiuk, A. Kompaniets, A. Tatarets","doi":"10.15407/cryo31.02.139","DOIUrl":"https://doi.org/10.15407/cryo31.02.139","url":null,"abstract":"In this paper we have studied the interaction of the mixtures of glycerol (GL) and its oxyethylated derivatives (OEG) with polymerization degree n = 3, 25 and 30 with dimethylacetamide (DMAc) in aqueous solution using 3-hydroxy-4´-(N, N dimethylaminoflavone) fluorescent probe. The combination of GL and its oxyethylated derivatives with DMAc was found to reduce the membranotropy of certain cryoprotective agents, forming a mixture. The combination of both GL and its low molecular weight derivative (OEGn=3) with DMAc reduced the membranotropy of the latter. At the same time, combining GL derivatives of high molecular weight (OEGn=25 and OEGn=30) with DMAc diminished the membranotropy of OEG. The OEGn=30 at concentrations above 1 wt.% was shown to form the micellar-type structures or micellar associates in aqueous solution. This enabled suggesting the membranotropic ability of high molecular weight OEG associates to be stipulated by possible interaction of their nonpolar segments with nonpolar sites on biomembrane surface. Structural rearrangements of molecular associates in aqueous solutions of low and high molecular weight cryoprotectant mixtures were designated as the experimentally established mechanism of cytotoxicity reduction in combined cryoprotective media.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45277101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of Osmotic Responses of Sweet Potato Meristems in Cryoprotective Solutions 甘薯分生组织在低温保护溶液中渗透反应的发现

Problems of Cryobiology and Cryomedicine Pub Date : 2021-06-25 DOI: 10.15407/cryo31.02.180

N. Shevchenko, G. Kovalenko, I. Kovalenko, T. Stribul

{"title":"Discovery of Osmotic Responses of Sweet Potato Meristems in Cryoprotective Solutions","authors":"N. Shevchenko, G. Kovalenko, I. Kovalenko, T. Stribul","doi":"10.15407/cryo31.02.180","DOIUrl":"https://doi.org/10.15407/cryo31.02.180","url":null,"abstract":"Батат (Ipomoea batatas L.) вирощується у багатьох регіонах світу та за виробництвом посідає сьоме місце серед найважливіших харчових культур. Вірусні захворювання (зокрема Sweet potato feathery mottle virus та Sweet potato chlorotic stunt virus) перешкоджають одержанню великих урожаїв цієї рослини [1, 4, 5]. Відомо, що батат розмножується вегетативно, тому постійне постачання безвірусного посадкового матеріалу має вирішальне значення для його сталого виробництва. Культуру меристем широко використовують для отримання безвірусних клонів культурних рослин. Ефективність видалення вірусів залежить від розміру верхівкових меристем: чим менший розмір зразка, тим більша ймовірність отримання безвірусної рослини. Останнім часом кріоконсервування вважається ефективним методом елімінації вірусів з рослинного матеріалу [5]. За даними літератури життєздатність меристем батату після низькотемпературного впливу не перевищує 40% [3]. Це може бути пов’язане з тим, що термін експозиції зразків у кріозахисних розчинах є або недостатнім для ефективної дегідратації, або, навпаки, занадто тривалим, що призводить до прояву токсичної дії хімічних сполук. Отже, підбір кріозахисного середовища та термінів експозиції меристем – надзвичайно актуальна проблема. Метою даної роботи було дослідження осмотичних реакцій меристем батату у кріозахисних розчинах для визначення терміну експозиції з максималь-","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44459734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Use of Modern Cryomethods in Combined Surgical Treatment of Patients with Purulent Chronic Wounds 现代冷冻方法在化脓性慢性伤口联合手术治疗中的应用

Problems of Cryobiology and Cryomedicine Pub Date : 2021-06-25 DOI: 10.15407/cryo31.02.109

K. Goltsev, I. Kryvoruchko, G. Goltsev, K. Parkhomenko, E. Horoshun

引用次数: 0

Impact of Biologically Active Complexes of L. rhamnosus GG and S. boulardii After Storage Under Low Temperatures on Biofilm Forming Ability in Corynebacteria 鼠李糖乳杆菌GG和布拉氏乳杆菌生物活性复合物低温贮藏对棒状杆菌生物膜形成能力的影响

Problems of Cryobiology and Cryomedicine Pub Date : 2021-06-25 DOI: 10.15407/cryo31.02.127

O. Isayenko, O. Knysh, M. Popov, V. Minukhin, Eugeny Babych, O. Peretyatko

{"title":"Impact of Biologically Active Complexes of L. rhamnosus GG and S. boulardii After Storage Under Low Temperatures on Biofilm Forming Ability in Corynebacteria","authors":"O. Isayenko, O. Knysh, M. Popov, V. Minukhin, Eugeny Babych, O. Peretyatko","doi":"10.15407/cryo31.02.127","DOIUrl":"https://doi.org/10.15407/cryo31.02.127","url":null,"abstract":"This paper describes the temperature regimens and shelf life of biologically active complexes (structural components and metabolites) of Lactobacillus rhamnosus GG and Saccharomyces boulardii, which have antimicrobial activity and reduce biofi lm formation in pathogenic agents of Corynebacterium spp. Preservation of biological activity of complexes after 6-month storage (observation period) at (−23 ± 1)°C and for 60 days (observation period) under hypothermia at (4 ± 1)°C has been demonstrated. The degree of inhibition of biofi lm formation in pathogenic corynebacteria depended on sensitivity of the Corynebacterium spp. test-culture strain to products of microbial origin. A mixture of lactobacillus and saccharomycete metabolites displayed weak biofi lm formation by toxicogenic strains of corynebacteria (p < 0.05). Our fi ndings testifi ed to a possible use of biologically active substances, stored at 4 and −23°C in designing the L. rhamnosus GG and S. boulardii complexes and in technological processes of their production to prevent the persistence of the diphtheria pathogens.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44331053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental Cryoablation of Thyroid Gland with Propilthiouracyl-Induced Diffuse Hyperplasiae 丙硫脲基诱导甲状腺弥漫性增生的实验冷冻消融

Problems of Cryobiology and Cryomedicine Pub Date : 2021-06-25 DOI: 10.15407/cryo31.02.168

K. Pobielienskyi, O. Pakhomov, Gurina Tetyana Gurina Tetyana, Liliia Pobielienska, Ievgen Legach Ievgen, G. Bozhok

{"title":"Experimental Cryoablation of Thyroid Gland with Propilthiouracyl-Induced Diffuse Hyperplasiae","authors":"K. Pobielienskyi, O. Pakhomov, Gurina Tetyana Gurina Tetyana, Liliia Pobielienska, Ievgen Legach Ievgen, G. Bozhok","doi":"10.15407/cryo31.02.168","DOIUrl":"https://doi.org/10.15407/cryo31.02.168","url":null,"abstract":"Cryoablation under endoscopic control is considered to be a promising approach in therapy of benign nodules of thyroid gland (TG). However, pathologically altered TG tissue differs in thermal conductivity and heat capacity from normal one, therefore the model experiments in animals are necessary to determine the cryoablation parameters. In this research, the changes of temperature during cryoablation of experimental rat TG under normal conditions and the one with propylthiouracil (PTU)-induced diffuse hyperplasia (DH) were comparatively assessed. TG was cryo-ablated in rats, previously received a 0.1% PTU solution within 90 days, using a copper cryoprobe, cooled to liquid nitrogen temperature. The process was controlled using thermocouples placed at different distances from the iceball. Differences between thermograms of intact TG tissue and the samples with PTU-induced DH were established. To achieve the destruction effect of TG with DH to a depth of more than 1 mm, the need of implementing two freeze-thaw cycles with 120-second cryoprobe exposure was proven.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45764705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0