Study of Interaction of Glycerol Cryoprotectant and Its Derivatives with Dimethylacetamide in Aqueous Solution Using Fluorescent Probes

Q4 Medicine

Problems of Cryobiology and Cryomedicine Pub Date : 2021-06-25 DOI:10.15407/cryo31.02.139

Tetiana Dyubko, V. Pivovarenko, V. Chekanova, Yuliya Pakhomova, Yana Gvozdiuk, A. Kompaniets, A. Tatarets

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Abstract

In this paper we have studied the interaction of the mixtures of glycerol (GL) and its oxyethylated derivatives (OEG) with polymerization degree n = 3, 25 and 30 with dimethylacetamide (DMAc) in aqueous solution using 3-hydroxy-4´-(N, N dimethylaminoflavone) fluorescent probe. The combination of GL and its oxyethylated derivatives with DMAc was found to reduce the membranotropy of certain cryoprotective agents, forming a mixture. The combination of both GL and its low molecular weight derivative (OEGn=3) with DMAc reduced the membranotropy of the latter. At the same time, combining GL derivatives of high molecular weight (OEGn=25 and OEGn=30) with DMAc diminished the membranotropy of OEG. The OEGn=30 at concentrations above 1 wt.% was shown to form the micellar-type structures or micellar associates in aqueous solution. This enabled suggesting the membranotropic ability of high molecular weight OEG associates to be stipulated by possible interaction of their nonpolar segments with nonpolar sites on biomembrane surface. Structural rearrangements of molecular associates in aqueous solutions of low and high molecular weight cryoprotectant mixtures were designated as the experimentally established mechanism of cytotoxicity reduction in combined cryoprotective media.

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用荧光探针研究甘油低温保护剂及其衍生物与二甲基乙酰胺在水溶液中的相互作用

本文用3-羟基-4´-（n，n-二甲基氨基黄酮）荧光探针研究了聚合度分别为3、25和30的甘油（GL）及其氧乙基化衍生物（OEG）与二甲基乙酰胺（DMAc）在水溶液中的相互作用。发现GL及其氧乙基化衍生物与DMAc的组合可以降低某些冷冻保护剂的膜促性，形成混合物。GL及其低分子量衍生物（OEGn=3）与DMAc的组合降低了后者的膜促性。同时，将高分子量的GL衍生物（OEGn=25和OEGn=30）与DMAc结合降低了OEG的膜促性。浓度高于1wt.%的OEGn=30显示在水溶液中形成胶束型结构或胶束缔合物。这表明高分子量OEG缔合物的膜营养能力是由其非极性片段与生物膜表面非极性位点的可能相互作用决定的。低分子量和高分子量冷冻保护剂混合物的水溶液中分子缔合物的结构重排被指定为在组合冷冻保护介质中实验建立的细胞毒性降低机制。

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来源期刊

Problems of Cryobiology and Cryomedicine Medicine-Medicine (miscellaneous)

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine. The journal covers all topics related to low temperature biology, medicine and engineering. These include but are not limited to: low temperature storage of biologicals (human, animal or plant cells, tissues, and organs), including preparation for storage, thawing/warming, cell and tissue culturing etc. response of biologicals to low temperature; cold adaptation of animals and plants; utilisation of low temperature in medicine; experimental and clinical transplantation, cell and tissue based therapies; developing of cryobiological and cryomedical devices; organisation and functioning of low temperature banks etc.