人工和自然冬眠大鼠和仓鼠血清的凝胶色谱检测

Q4 Medicine

Problems of Cryobiology and Cryomedicine Pub Date : 2021-09-25 DOI:10.15407/cryo31.03.191

O. Shylo, V. Lomako, O. Semenchenko

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引用次数: 0

摘要

本研究采用凝胶渗透色谱法研究了天然（NH）和人工冬眠（AH）条件下仓鼠和大鼠血液的分子组成。对照组在仓鼠中用5个分子级分表示，在大鼠中用7个。仓鼠和大鼠中分子量相似的组分的峰下面积在大鼠中较大。NH的特征是出现了新的级分（1350、2350和6350Da），并且对照级分的峰下面积增加（1140和1980Da）。大鼠的人工冬眠导致1140Da组分峰值下的面积增加，530Da组分的面积减少，1290Da消失，以及新组分（6508309502350和5110Da）的出现。AH后2小时，1140和1520Da级分的峰下面积较大，530Da级分的峰值下面积较小；650、2350和5110Da组分消失，1290Da重新出现，4030Da的新组分出现。24小时后发现5820和6530Da的新级分。在AH下的仓鼠中，1140、1600和3330Da峰值下的面积增加；以及在对照大鼠和AH后24小时出现5280Da的新峰值。

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Gel Chromatographic Examination of Serum of Rats and Hamsters Under Artificial and Natural Hibernation

In this study, molecular composition of hamster and rat blood was studied by gel permeation chromatography under natural (NH) and artificial hibernation (AH). The control group was represented by 5 fractions of molecules in hamsters and 7 in rats. The areas under peaks of the fractions similar in molecular weight in hamsters and rats were larger in rats. NH was characterized by appearance of new fractions (1,350, 2,350, and 6,350 Da) and an increase in areas under peaks of the control fractions (1,140 and 1,980 Da). Artificial hibernation in rats led to an increase in areas under peaks of 1,140 Da fraction, a decrease in that of 530 Da, and disappearance of 1290 Da, as well as the appearance of new fractions (650, 830, 950, 2350, and 5110 Da). Two hrs of later AH, the areas under peaks of 1,140 and 1,520 Da fractions were greater and that of 530 Da was lesser; 650, 2,350 and 5,110 Da fractions disappeared, 1,290 Da reappeared and new fraction of 4,030 Da appeared. New fractions of 5,820 and 6,530 Da were found 24 hrs later. In hamsters under AH, the areas under peaks of 1,140, 1,600, and 3,330 Da increased; as well as a new peak in 5,280 Da appeared, both in the control rats and those in 24 hrs after AH.

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来源期刊

Problems of Cryobiology and Cryomedicine Medicine-Medicine (miscellaneous)

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine. The journal covers all topics related to low temperature biology, medicine and engineering. These include but are not limited to: low temperature storage of biologicals (human, animal or plant cells, tissues, and organs), including preparation for storage, thawing/warming, cell and tissue culturing etc. response of biologicals to low temperature; cold adaptation of animals and plants; utilisation of low temperature in medicine; experimental and clinical transplantation, cell and tissue based therapies; developing of cryobiological and cryomedical devices; organisation and functioning of low temperature banks etc.