Impact of Biologically Active Complexes of L. rhamnosus GG and S. boulardii After Storage Under Low Temperatures on Biofilm Forming Ability in Corynebacteria

Q4 Medicine

Problems of Cryobiology and Cryomedicine Pub Date : 2021-06-25 DOI:10.15407/cryo31.02.127

O. Isayenko, O. Knysh, M. Popov, V. Minukhin, Eugeny Babych, O. Peretyatko

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引用次数: 0

Abstract

This paper describes the temperature regimens and shelf life of biologically active complexes (structural components and metabolites) of Lactobacillus rhamnosus GG and Saccharomyces boulardii, which have antimicrobial activity and reduce biofi lm formation in pathogenic agents of Corynebacterium spp. Preservation of biological activity of complexes after 6-month storage (observation period) at (−23 ± 1)°C and for 60 days (observation period) under hypothermia at (4 ± 1)°C has been demonstrated. The degree of inhibition of biofi lm formation in pathogenic corynebacteria depended on sensitivity of the Corynebacterium spp. test-culture strain to products of microbial origin. A mixture of lactobacillus and saccharomycete metabolites displayed weak biofi lm formation by toxicogenic strains of corynebacteria (p < 0.05). Our fi ndings testifi ed to a possible use of biologically active substances, stored at 4 and −23°C in designing the L. rhamnosus GG and S. boulardii complexes and in technological processes of their production to prevent the persistence of the diphtheria pathogens.

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鼠李糖乳杆菌GG和布拉氏乳杆菌生物活性复合物低温贮藏对棒状杆菌生物膜形成能力的影响

本文介绍了鼠李糖乳杆菌GG和博拉氏酵母菌的生物活性复合物(结构成分和代谢物)的温度方案和保质期，这两种复合物具有抗菌活性，可以减少棒状杆菌病原菌的生物膜形成，并在(- 23±1)°C下保存6个月(观察期)，在(4±1)°C低温下保存60天(观察期)。致病棒状杆菌对生物膜形成的抑制程度取决于棒状杆菌试验培养菌株对微生物源产物的敏感性。乳酸菌和酵母菌混合代谢产物在毒理菌株杆状菌的作用下形成的生物膜较弱(p < 0.05)。我们的研究结果证明，在4°C和- 23°C下储存的生物活性物质可能用于设计鼠李糖乳杆菌GG和博氏菌复合物，并在其生产的技术过程中防止白喉病原体的持续存在。

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来源期刊

Problems of Cryobiology and Cryomedicine Medicine-Medicine (miscellaneous)

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine. The journal covers all topics related to low temperature biology, medicine and engineering. These include but are not limited to: low temperature storage of biologicals (human, animal or plant cells, tissues, and organs), including preparation for storage, thawing/warming, cell and tissue culturing etc. response of biologicals to low temperature; cold adaptation of animals and plants; utilisation of low temperature in medicine; experimental and clinical transplantation, cell and tissue based therapies; developing of cryobiological and cryomedical devices; organisation and functioning of low temperature banks etc.