二甲基亚砜对L929细胞和L929基球体的渗透动力学

Q4 Medicine

Problems of Cryobiology and Cryomedicine Pub Date : 2021-12-25 DOI:10.15407/cryo31.04.316

A. Moisieiev, I. Kovalenko, S.Ju. Kovalenko, G. Bozhok, O. Gordienko

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引用次数: 2

摘要

本研究提出了一种利用传质物理模型和数学模型计算低温保护剂介质中多细胞结构可观渗透系数的算法。测定了不同温度下L929细胞的表面积体积比，计算了细胞膜表面积的热膨胀系数(β = 2.7 × 10-3 /°C)。测定了L929细胞及其球体的渗透失活体积。通过相对体积变化的动态曲线，得到了L929细胞和toto球体对DMSO的过滤系数和渗透系数。计算出的参数在单个细胞中是最高的，而在球体中的细胞随着其位置深度的增加而显著(p <0.05)降低，这种降低可能是由于球体中细胞用于细胞外物质渗透的可用表面的减少所规定的。本研究获得的球类材料的渗透性特征可用于制定球类材料的最佳低温保存方案。

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Dynamics of Dimethyl Sulfoxide Penetration Into L929 Cells and L929-Based Spheroids

The study proposes an algorithm for calculating of appreciable permeability coefficients for multicellular structures in a cryoprotectant medium using physical and mathematical model of mass transfer. The values of surface-area-to-volume ratio for L929 cells at different temperatures were determined and the thermal expansion coefficient of the surface area of cell membranes was calculated (β = 2.7 × 10-3 /°C). The osmotically inactive volume for L929 cells and their spheroids was determined. Filtration and permeability coefficients to DMSO for L929 cells and in toto spheroids were found from the dynamic curves of relative volume change. The calculated parameters are the highest for individual cells and significantly (p <0.05) decrease for cells in the spheroids with increasing depth of their location, this reduction may be stipulated by a decrease in the available surface of cells in the spheroids for the penetration of extracellular substances. Obtained in this research permeability characteristics of spheroids can be used to develop optimal cryopreservation regimens for them.

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来源期刊

Problems of Cryobiology and Cryomedicine Medicine-Medicine (miscellaneous)

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine. The journal covers all topics related to low temperature biology, medicine and engineering. These include but are not limited to: low temperature storage of biologicals (human, animal or plant cells, tissues, and organs), including preparation for storage, thawing/warming, cell and tissue culturing etc. response of biologicals to low temperature; cold adaptation of animals and plants; utilisation of low temperature in medicine; experimental and clinical transplantation, cell and tissue based therapies; developing of cryobiological and cryomedical devices; organisation and functioning of low temperature banks etc.