Theoretical Estimation of Optimal Linear Cooling Rate for PK-15 Cell Suspension

Q4 Medicine

Problems of Cryobiology and Cryomedicine Pub Date : 2021-09-25 DOI:10.15407/cryo31.03.214

O. Gordienko, I. Kovalenko, S.Ju. Kovalenko, L. Kuleshova, O. Todrin

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引用次数: 1

Abstract

Preservation of cells during crystallization of the cell suspension is influenced by two types of damaging factors. The first type of cryoinjury occurs during the crystallization of the extracellular environment and is caused by dehydration of cells, increasing the concentration and ionic strength of extracellular and intracellular solutions. As the cooling rate rises, the damage rate of the first type decreases as a result of the reduced time of action of damaging factors. The second type of cryoinjury is intracellular crystallization, the probability of which enhances at high cooling rates, is considered the most destructive to cells. The optimal linear cooling rate for PK-15 cells is determined using a physico-mathematical model, which describes the probability of cryoinjury of cells in the linear freezing mode and is based on the two-factor theory of cryoinjury, thermodynamic theory of homogeneous crystallization and general theory of activation-type processes. The findings have shown that within the range of cooling rates < 0.5 °C/min the cryoinjury of PK-15 cells occurs mainly due to the effects of the solution, and at cooling rates > 2.5 °C/min this was mainly resulted from an intracellular crystallization. The dependence of the percentage of damaged cells on the cooling rate has a relatively wide minimum within the range of cooling rates of 0.5 °C/min… 2.5 °C/min.

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PK-15细胞悬浮液最佳线性冷却速率的理论估计

细胞悬浮液结晶过程中细胞的保存受到两类破坏因素的影响。第一种类型的低温损伤发生在细胞外环境的结晶过程中，是由细胞脱水引起的，细胞外和细胞内溶液的浓度和离子强度增加。随着冷却速率的增大，由于损伤因素作用时间的缩短，第一类损伤速率降低。第二种类型的低温损伤是细胞内结晶，其可能性在高冷却速率下增强，被认为是对细胞最具破坏性的。基于冷冻损伤双因素理论、均匀结晶热力学理论和活化型过程的一般理论，利用描述细胞在线性冷冻模式下冷冻损伤概率的物理数学模型确定了PK-15细胞的最佳线性冷却速率。结果表明，在冷却速率< 0.5°C/min范围内，PK-15细胞的低温损伤主要是由于溶液的作用，而在冷却速率> ~ 2.5°C/min范围内，低温损伤主要是由于细胞内结晶引起的。在0.5°C/min ~ 2.5°C/min的冷却速率范围内，损坏电池的百分比对冷却速率的依赖性相对较小。

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来源期刊

Problems of Cryobiology and Cryomedicine Medicine-Medicine (miscellaneous)

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine. The journal covers all topics related to low temperature biology, medicine and engineering. These include but are not limited to: low temperature storage of biologicals (human, animal or plant cells, tissues, and organs), including preparation for storage, thawing/warming, cell and tissue culturing etc. response of biologicals to low temperature; cold adaptation of animals and plants; utilisation of low temperature in medicine; experimental and clinical transplantation, cell and tissue based therapies; developing of cryobiological and cryomedical devices; organisation and functioning of low temperature banks etc.