Problems of Cryobiology and Cryomedicine最新文献_第8页

Osmotic Resistance of Rat’s Erythrocytes After Local Cold Injury 大鼠局部冷损伤后红细胞的渗透阻力

Problems of Cryobiology and Cryomedicine Pub Date : 2022-03-30 DOI: 10.15407/cryo32.01.024

G. Kovalov, O. Chabanenko, N. Orlova, N. Shpakova

引用次数: 0

Lyophilized Human Cord Blood Leukoconcentrate to Treat Brain Ischemia in Rats 冷冻人脐血浓缩白细胞治疗大鼠脑缺血

Problems of Cryobiology and Cryomedicine Pub Date : 2022-03-30 DOI: 10.15407/cryo32.01.044

V. Lebedinets, L. Ostankova, M. Bondarovych, O. Lutsenko, D. Lebedinets, Igor Grisha, M. Ostankov, K. Parkhomenko, A. Goltsev

引用次数: 2

Opportunities of Electron Microscopy When Solving Cryobiological Tasks. Retrospective Analysis 电子显微镜在解决低温生物学任务中的机会。回顾性分析

Problems of Cryobiology and Cryomedicine Pub Date : 2022-03-30 DOI: 10.15407/cryo32.01.003

N. Repin, L. Marchenko, T. Govorukha, A. Goltsev

{"title":"Opportunities of Electron Microscopy When Solving Cryobiological Tasks. Retrospective Analysis","authors":"N. Repin, L. Marchenko, T. Govorukha, A. Goltsev","doi":"10.15407/cryo32.01.003","DOIUrl":"https://doi.org/10.15407/cryo32.01.003","url":null,"abstract":"The history of the development and use of low-temperature electron microscopic methods of freeze-fracture, freeze-substitution and others in cryobiological research at the Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine is presented in this article. These methods’ possibilities in studying the processes of crystal formation in cryoprotectant solutions, cell suspensions, and tissues under various freezing conditions are demonstrated. Some results of the analysis of ultrastructural changes in biological systems of various organization levels, at different stages of ontogenesis, under the influence of cooling in a wide temperature range (from 37 to –196°C) are presented. The use of a high resolution electron microscopic method in combination with an accessory technical equipment and some methodological techniques allowed to obtain fundamental results important for cryobiology on ice crystals formation and localization in the intracellular volume, the temperature-dependent transmembrane proteins redistribution, changes in the ultrastructure of erythrocytes and their membranes during hypothermic storage.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47602241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Freeze-Drying Improved the Stability of Tuberculin Purified Protein Derivative (PPD) in Mammals 冷冻干燥提高结核菌素纯化蛋白衍生物（PPD）在哺乳动物体内的稳定性

Problems of Cryobiology and Cryomedicine Pub Date : 2022-03-30 DOI: 10.15407/cryo32.01.063

A. Zavhorodnii, V. Bilushko, A. Paliy, M. Kalashnyk, N. Kalashnyk, A. Paliy

引用次数: 0

Can Sea Urchin Eggs Shelf-Life Be Extended by Cold Storage? 冷藏能延长海胆卵的保质期吗?

Problems of Cryobiology and Cryomedicine Pub Date : 2022-03-30 DOI: 10.15407/cryo32.01.068

Sara Campos, E. Paredes, Jesús Troncoso Jesús Troncoso

引用次数: 1

Effect of Leu-Enkephalin (Dalargin) on Apoptosis and Necrosis of Leukocytes After Cold Stress 亮脑啡肽(Dalargin)对冷应激后白细胞凋亡和坏死的影响

Problems of Cryobiology and Cryomedicine Pub Date : 2022-03-30 DOI: 10.15407/cryo32.01.014

N. Moisieieva, O. Gulevskyy, O. Gorina

{"title":"Effect of Leu-Enkephalin (Dalargin) on Apoptosis and Necrosis of Leukocytes After Cold Stress","authors":"N. Moisieieva, O. Gulevskyy, O. Gorina","doi":"10.15407/cryo32.01.014","DOIUrl":"https://doi.org/10.15407/cryo32.01.014","url":null,"abstract":"The effect of synthetic leu-enkephalin (dalargin) on apoptosis and necrosis in leukocytes after cold stress on the morphological characteristics of cells and using fluorescent dyes Hoechst 33342 as well as propidium iodide was studied. The degree of cold stress influence depending on the duration of incubation of cell suspension at 0–2 and 37°C on the initiation of apoptosis and necrosis on the morphological characteristics of cells was determined. It has been proven that the ratio of apoptotic cells to necrotic ones was maximal after the use of the regimen as follows: preliminary incubation of leukocytes at 37°C – 15 min, cell exposure at 0–2°C – 15 min and subsequent incubation of leukocytes at 37°C. It has been confirmed that pre-incubation of cells with neuropeptide to cold stress affects the effectiveness of the protective action of dalargin, in particular, contributes to a significant (p ≤ 0.05) reduction in the percentage of leukocytes with morphological signs of apoptosis and necrosis. It was found that a consistent increase in temperature after cold stress to 37°C did not affect the percentage of cells with morphological signs of apoptosis and necrosis. It has been validated that the percentage of leukocytes with the apoptosis and necrosis signs after cold stress was significantly reduced after incubation of leukocytes with dalargin at a concentration of 10–9 mol/l.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43818219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryopreservation as Biotechnological Application of Dendritic Cells in Clinical Practice 冷冻保存作为树突状细胞的生物技术应用

Problems of Cryobiology and Cryomedicine Pub Date : 2021-12-25 DOI: 10.15407/cryo31.04.289

A. Goltsev, K. Yampolska, H. Kisielova, Maksym Оstankov, T. Dubrava, N. Babenko, Yuliya Gaevska, M. Bondarovych

{"title":"Cryopreservation as Biotechnological Application of Dendritic Cells in Clinical Practice","authors":"A. Goltsev, K. Yampolska, H. Kisielova, Maksym Оstankov, T. Dubrava, N. Babenko, Yuliya Gaevska, M. Bondarovych","doi":"10.15407/cryo31.04.289","DOIUrl":"https://doi.org/10.15407/cryo31.04.289","url":null,"abstract":"An important component of the pathogenesis of autoimmune diseases is the immune system deregulation as an impaired tolerance to its own antigens by reducing the content of T-regulatory cells. Their formation is closely related to the function of dendritic cells (DCs), so in autoimmune diseases the use of DCs with tolerogenic potential is promising for the restoration of antigen-specific tolerance. Recently, the issue of establishing the banks of tolerogenic DCs for clinical use, which involves their cryopreservation, has been actively discussed. To date, there is no common protocol for DCs freezing, which would take into account the different sources of their obtaining, the initial structural and functional state before freezing, composition of cryopreservation media and other factors. The review summarizes experimental data on cryopreservation of peripheral blood and bone marrow mononuclear cells or monocytes. The potential for their further ex vivo differentiation into DCs to ensure the stability of immature phenotype and tolerogenic function has been studied.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49305403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blood Characteristics in Dogs During Treatment of Babesiosis Using Transfusion of Cryopreserved Erythrocytes 输血冷冻红细胞治疗巴贝斯虫病期间狗的血液特征

Problems of Cryobiology and Cryomedicine Pub Date : 2021-12-25 DOI: 10.15407/cryo31.04.304

Оlha Denysova, G. Zhegunov, T. Yakimenko, N. Hladka, V. Prichodchenko, Olha Bobrutska

引用次数: 0

Endocrine Function of Ovarian Tissue Grafts After Normo- and Hypothermic Storage 卵巢组织移植物常温和低温保存后的内分泌功能

Problems of Cryobiology and Cryomedicine Pub Date : 2021-12-25 DOI: 10.15407/cryo31.04.326

V. Kiroshka, Yu. O. Bozhkova, A. Gavas, I. Rula, T. Bondarenko, G. Semko, V. Pyatikop

{"title":"Endocrine Function of Ovarian Tissue Grafts After Normo- and Hypothermic Storage","authors":"V. Kiroshka, Yu. O. Bozhkova, A. Gavas, I. Rula, T. Bondarenko, G. Semko, V. Pyatikop","doi":"10.15407/cryo31.04.326","DOIUrl":"https://doi.org/10.15407/cryo31.04.326","url":null,"abstract":"Morphological characteristics and function of ovarian tissue after 2–120 hrs of storage at 4 and 37°C in incubation media with different composition (mannitol-containing solution (MСS) and phosphate buffered saline (PBS)) were investigated. The function of ovarian tissue after normo- and hypothermic storage was studied by heterotopic transplantation to recipient animals simultaneously with bilateral ovariectomy. After 2-hour storage at 37ºC and 24-hour one at 4ºC in all the studied media, the estradiol and progesterone levels in plasma of recipient animals were experimentally proved to be 18.2–27.5 and 13.5–18.5 ng/ml, respectively, (after fresh tissue transplantation these values for estradiol and progesterone made (28.1 ± 3.6) pg/ml and (19.1 ± 2.8) ng/ml, respectively). Prolongation of tissue storage time at 37ºC increased the number of degenerative follicles, augmented TBARS concentration and reduced sex hormone levels after transplantation using both MCS and PBS. A protective effect of MCS only, manifested in endocrine function restoration in recipient animals after transplantation ((13.5 ± 2.5) pg/ml of estradiol) was found after 48 hrs of ovarian tissue hypothermic storage. If PBS was used, the estradiol level was within the indices for ovariectomized animals ((7.1 ± 1.5 (pg/ml)).","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49355585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Freezing Regimens and Gel Carrier Composition Influence Safety of Saccharomyces boulardii Immobilized Yeast Cells 冷冻方案和凝胶载体组成对博氏酵母固定化酵母细胞安全性的影响

Problems of Cryobiology and Cryomedicine Pub Date : 2021-12-25 DOI: 10.15407/cryo31.04.343

I. Vysekantsev, V. Martsenyuk, I. Buriak, T. Gurina

{"title":"Freezing Regimens and Gel Carrier Composition Influence Safety of Saccharomyces boulardii Immobilized Yeast Cells","authors":"I. Vysekantsev, V. Martsenyuk, I. Buriak, T. Gurina","doi":"10.15407/cryo31.04.343","DOIUrl":"https://doi.org/10.15407/cryo31.04.343","url":null,"abstract":"The effect of DMSO and sucrose added to alginate gel on the viability of immobilized Saccharomyces boulardii yeast cells after rapid and slow cooling to –196 °C has been studied. Thermomechanical analysis revealed the inflection of phase transitions corresponding to each component of the solutions when cooling those of 1% sodium alginate contained 20% sucrose or 5% DMSO down to –196 °C. Phase transitions caused by the presence of DMSO and sucrose in sodium alginate solutions were observed at lower temperatures than in impurity-free sodium alginate solution. In experiments to study the viability of free and immobilized yeast cells after freezing, it was found that the highest viability of free cells suspended in solutions of sodium alginate, DMSO, sucrose, and the cells immobilized in gel granules, was provided by a cooling rate of 1 deg/min followed by immersion in liquid nitrogen. After adding DMSO and sucrose separately to the alginate gel, as well as their combinations, the viability of immobilized cells increased. The maximum viability of immobilized cells was found in gel granules, which contained the following combinations of cryoprotectants: 5% DMSO and 10% sucrose, 5% DMSO and 20% sucrose, 10% DMSO and 10% sucrose and 10% DMSO and 20% sucrose.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48924808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0