Cryopreservation as Biotechnological Application of Dendritic Cells in Clinical Practice

Q4 Medicine

Problems of Cryobiology and Cryomedicine Pub Date : 2021-12-25 DOI:10.15407/cryo31.04.289

A. Goltsev, K. Yampolska, H. Kisielova, Maksym Оstankov, T. Dubrava, N. Babenko, Yuliya Gaevska, M. Bondarovych

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引用次数: 0

Abstract

An important component of the pathogenesis of autoimmune diseases is the immune system deregulation as an impaired tolerance to its own antigens by reducing the content of T-regulatory cells. Their formation is closely related to the function of dendritic cells (DCs), so in autoimmune diseases the use of DCs with tolerogenic potential is promising for the restoration of antigen-specific tolerance. Recently, the issue of establishing the banks of tolerogenic DCs for clinical use, which involves their cryopreservation, has been actively discussed. To date, there is no common protocol for DCs freezing, which would take into account the different sources of their obtaining, the initial structural and functional state before freezing, composition of cryopreservation media and other factors. The review summarizes experimental data on cryopreservation of peripheral blood and bone marrow mononuclear cells or monocytes. The potential for their further ex vivo differentiation into DCs to ensure the stability of immature phenotype and tolerogenic function has been studied.

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冷冻保存作为树突状细胞的生物技术应用

自身免疫性疾病发病机制的一个重要组成部分是免疫系统失调，这是一种通过减少T调节细胞的含量来损害对自身抗原的耐受性。它们的形成与树突状细胞（DC）的功能密切相关，因此在自身免疫性疾病中，使用具有耐受潜力的DC有望恢复抗原特异性耐受。最近，建立临床使用的耐受性DC库的问题，包括其冷冻保存，已经被积极讨论。到目前为止，还没有一个通用的DC冷冻方案，该方案将考虑其获得的不同来源、冷冻前的初始结构和功能状态、冷冻保存介质的组成和其他因素。综述了外周血和骨髓单核细胞或单核细胞冷冻保存的实验数据。已经研究了它们进一步体外分化为DC以确保未成熟表型和耐受功能的稳定性的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Problems of Cryobiology and Cryomedicine Medicine-Medicine (miscellaneous)

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine. The journal covers all topics related to low temperature biology, medicine and engineering. These include but are not limited to: low temperature storage of biologicals (human, animal or plant cells, tissues, and organs), including preparation for storage, thawing/warming, cell and tissue culturing etc. response of biologicals to low temperature; cold adaptation of animals and plants; utilisation of low temperature in medicine; experimental and clinical transplantation, cell and tissue based therapies; developing of cryobiological and cryomedical devices; organisation and functioning of low temperature banks etc.