Opportunities of Electron Microscopy When Solving Cryobiological Tasks. Retrospective Analysis

Q4 Medicine

Problems of Cryobiology and Cryomedicine Pub Date : 2022-03-30 DOI:10.15407/cryo32.01.003

N. Repin, L. Marchenko, T. Govorukha, A. Goltsev

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Abstract

The history of the development and use of low-temperature electron microscopic methods of freeze-fracture, freeze-substitution and others in cryobiological research at the Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine is presented in this article. These methods’ possibilities in studying the processes of crystal formation in cryoprotectant solutions, cell suspensions, and tissues under various freezing conditions are demonstrated. Some results of the analysis of ultrastructural changes in biological systems of various organization levels, at different stages of ontogenesis, under the influence of cooling in a wide temperature range (from 37 to –196°C) are presented. The use of a high resolution electron microscopic method in combination with an accessory technical equipment and some methodological techniques allowed to obtain fundamental results important for cryobiology on ice crystals formation and localization in the intracellular volume, the temperature-dependent transmembrane proteins redistribution, changes in the ultrastructure of erythrocytes and their membranes during hypothermic storage.

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电子显微镜在解决低温生物学任务中的机会。回顾性分析

本文介绍了乌克兰国家科学院低温生物学和低温医学问题研究所低温电镜冷冻断裂、冷冻替代等方法在低温生物学研究中的发展和使用历史。这些方法在研究冷冻保护剂溶液、细胞悬浮液和组织在各种冷冻条件下晶体形成过程中的可能性被证明。本文介绍了在37 ~ -196℃的低温条件下，不同组织水平的生物系统在个体发生的不同阶段的超微结构变化的分析结果。使用高分辨率电子显微镜方法，结合辅助技术设备和一些方法学技术，可以获得对低温生物学至关重要的基本结果，包括细胞内体积中冰晶的形成和定位，温度依赖性跨膜蛋白的再分配，低温储存期间红细胞及其膜的超微结构的变化。

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来源期刊

Problems of Cryobiology and Cryomedicine Medicine-Medicine (miscellaneous)

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine. The journal covers all topics related to low temperature biology, medicine and engineering. These include but are not limited to: low temperature storage of biologicals (human, animal or plant cells, tissues, and organs), including preparation for storage, thawing/warming, cell and tissue culturing etc. response of biologicals to low temperature; cold adaptation of animals and plants; utilisation of low temperature in medicine; experimental and clinical transplantation, cell and tissue based therapies; developing of cryobiological and cryomedical devices; organisation and functioning of low temperature banks etc.