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Problems of Cryobiology and Cryomedicine最新文献

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Pituitary-Thyroid System in Rats of Different Ages Under Desynchronosis, Whole-Body Cryostimulation, and Cord Blood Injection 不同年龄大鼠垂体-甲状腺系统在失同步、全身冷冻刺激和脐带血注射下的变化
Problems of Cryobiology and Cryomedicine Pub Date : 2022-09-30 DOI: 10.15407/cryo32.03.196
V. Lomako, O. Shylo, L. Samokhina, D. Lutsenko
{"title":"Pituitary-Thyroid System in Rats of Different Ages Under Desynchronosis, Whole-Body Cryostimulation, and Cord Blood Injection","authors":"V. Lomako, O. Shylo, L. Samokhina, D. Lutsenko","doi":"10.15407/cryo32.03.196","DOIUrl":"https://doi.org/10.15407/cryo32.03.196","url":null,"abstract":"The state of the pituitary-thyroid system central and peripheral links (according to the levels of thyroid stimulating hormone (TSH) and thyroid hormones T4 and T3 in blood serum) were studied in 6- and 18-month-old rats under initiated circadian desynchronosis (CD) by the light regime inverting. The CD was simulated against the background of separate and combined eff ect of both the whole body cryostimulation (–120°C) (WBC) and injections of the nucleated cells (NCs) of human cord blood, applied preventively (for a day and week, respectively). In 6-month-old rats, the TSH level decreased under the CD and its initiation after separate NCs administration and combined with WBC (by 3.4, 3.9 and 6.3 times, respectively) and remained unchanged under CD against the WBC. In 18-month-old rats the TSH level was reduced only at CD by 1.8 times. The T4 level in rats of both age groups did not change under the CD as well as during its development against the background of a combined effect of WBC and NCs administration in 6-month-old animals and against the NCs injection in 18 months-old rats. The T4 level in rats of both age groups decreased at the background of separate effect of the WBC (by 1.6 and 1.4 times, respectively). In 18-month-old animals, the T4 increased under the CD development after a combined effect of WBC and NCs injection by 1.3 times. No changes in the T3 level were found.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45201216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction of Cytokine Profile in Autoimmune Diseases with Cryopreserved Embryofetoplacental Complex Products 用冷冻胚胎胎盘复合物制品校正自身免疫性疾病的细胞因子谱
Problems of Cryobiology and Cryomedicine Pub Date : 2022-06-30 DOI: 10.15407/cryo32.02.121
A. Goltsev, O. Lutsenko, Kateryna Yampolska, Yuliia Gaevska, M. Bondarovych, Lyudmyla Ostankova, L. Sokil, L. Stepaniuk, Ihor Grisha
{"title":"Correction of Cytokine Profile in Autoimmune Diseases with Cryopreserved Embryofetoplacental Complex Products","authors":"A. Goltsev, O. Lutsenko, Kateryna Yampolska, Yuliia Gaevska, M. Bondarovych, Lyudmyla Ostankova, L. Sokil, L. Stepaniuk, Ihor Grisha","doi":"10.15407/cryo32.02.121","DOIUrl":"https://doi.org/10.15407/cryo32.02.121","url":null,"abstract":"In this work, we experimentally substantiated the possibility of correcting the cytokine profile to reduce the inflammation in mice with adjuvant arthritis (AA) via combined use of lyophilized human cord blood leukoconcentrate (lHCBL) and cryopreserved human placental extract (cHPE). Adjuvant arthritis was modelled in CBA/H mice. To day 7 after pathology induction, the animals were injected with lHCBL and cHPE singly or in combination. To days 14 and 28 of observation, all the introduced products showed a therapeutic effect. To 28 day of observation, the efficiency of combined use of lHCBL and cHPE was 3.1 and 2.5 times higher vs. the monotherapy (respectively), likely resulting from their synergy in respect to a decrease in arthritis index, TNFα concentration, impact of lHCBL and cHPE on IFNγ and IL-6 levels, correspondingly.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44995432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Physiological Activity of Salmonella spp. Bacteria After Lyophilization and Rehydration 冻干和复水后沙门氏菌的生理活性
Problems of Cryobiology and Cryomedicine Pub Date : 2022-06-30 DOI: 10.15407/cryo32.02.158
M. Romanko, V. Ushkalov, A. Paliy, A. Paliy, R. Petrov, L. Livoshchenko, Ye.M. Livoshchenko
{"title":"Physiological Activity of Salmonella spp. Bacteria After Lyophilization and Rehydration","authors":"M. Romanko, V. Ushkalov, A. Paliy, A. Paliy, R. Petrov, L. Livoshchenko, Ye.M. Livoshchenko","doi":"10.15407/cryo32.02.158","DOIUrl":"https://doi.org/10.15407/cryo32.02.158","url":null,"abstract":"","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49036200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Evaluation of Mitotic Activity of Bone Marrow Under Short-Term Hypothermia 短期低温下骨髓有丝分裂活性的评价
Problems of Cryobiology and Cryomedicine Pub Date : 2022-06-30 DOI: 10.15407/cryo32.02.081
R. Aminov, Оleksandr Frolov, A. Aminova, L. Makyeyeva
{"title":"Evaluation of Mitotic Activity of Bone Marrow Under Short-Term Hypothermia","authors":"R. Aminov, Оleksandr Frolov, A. Aminova, L. Makyeyeva","doi":"10.15407/cryo32.02.081","DOIUrl":"https://doi.org/10.15407/cryo32.02.081","url":null,"abstract":"In this research, we have studied the mitotic activity of bone marrow when applying medicinal leeches and short-term hypothermia. The bone marrow mitotic indices were compared within an immediate examination of the thigh removed from the body and during a short-term hypothermia. As a result, no statistical differences were found between the mitotic levels during immediate examination and a short-term hypothermia in the intact animals and those after medicinal leech application, that evidenced to a hypothermal fixation of initial level of this index, preservation of biomembrane physiological state prior to hypotonic stress and cell morphology. The used here short-term hypothermia on bone marrow cells may be included in the multi-profile algorithm of laboratory analysis when studying a mitotic index in actively proliferating cells.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43354240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolism Reactivation in Erythrocytes Cryopreserved with Polyethylene Glycol: Protein State and Reactive Oxygen Species Production 聚乙二醇冷冻保存红细胞的代谢再活化：蛋白质状态和活性氧的产生
Problems of Cryobiology and Cryomedicine Pub Date : 2022-06-30 DOI: 10.15407/cryo32.02.092
N. Zemlianskykh, L. Babijchuk
{"title":"Metabolism Reactivation in Erythrocytes Cryopreserved with Polyethylene Glycol: Protein State and Reactive Oxygen Species Production","authors":"N. Zemlianskykh, L. Babijchuk","doi":"10.15407/cryo32.02.092","DOIUrl":"https://doi.org/10.15407/cryo32.02.092","url":null,"abstract":"Production of reactive oxygen species (ROS) and protein state of membrane-cytoskeleton complex (MCC) in the erythrocytes, subjected to freeze-thawing with polyethylene glycol (PEG) and subsequently transferred into physiological conditions in vitro after cryoprotective agent removal were examined. Results of SDS-PAGE with β-mercaptoethanol showed a decrease in spectrin content in the MCC profile of cryopreserved erythrocytes. The using of diamide revealed an increase in the accessibility of –SH groups of spectrin for the reagent and an enhanced level of high molecular weight polypeptide complexes relative to the control. A decrease in the content of protein band 8 (peroxiredoxin) in the MCC of cryopreserved erythrocytes revealed by SDS-PAGE with β-mercaptoethanol is apparently associated with a change in its localization caused by an increased ROS production at the previous stages of cryopreservation. At the same time, the absence of significant changes in the ROS production relative to the control upon the metabolism reactivation in erythrocytes indicated the ability of cryopreserved cells to control the oxidative processes during short-term incubation.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49612498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Low-Temperature Storage of Placenta Affects Anti-Inflammatory and Wound Healing Properties of Its Extracts 胎盘低温贮藏对其提取物抗炎和创伤愈合性能的影响
Problems of Cryobiology and Cryomedicine Pub Date : 2022-06-30 DOI: 10.15407/cryo32.02.144
O. Bobrova, Ye. V. V. Naumenko, Myroslav Shchetinskyi, S. Narozhnyi, O. Nardid, М. Kalashnykova, Irina Shchetinska
{"title":"Low-Temperature Storage of Placenta Affects Anti-Inflammatory and Wound Healing Properties of Its Extracts","authors":"O. Bobrova, Ye. V. V. Naumenko, Myroslav Shchetinskyi, S. Narozhnyi, O. Nardid, М. Kalashnykova, Irina Shchetinska","doi":"10.15407/cryo32.02.144","DOIUrl":"https://doi.org/10.15407/cryo32.02.144","url":null,"abstract":"Here we have studied the effect of low-temperature storage of the placenta at different temperatures on anti-inflammatory activity of its extracts in cell models of erythrocyte thermohemolysis and adenosine-5’-diphosphate-induced platelet aggregation. The wound-healing effect of cryopreserved placental extracts was also evaluated in rat thermal burn model. When preserving placenta for up to a month, the temperature of storage was shown to cause no significant impact on the percentage of inhibition of erythrocyte thermohemolysis and platelet aggregation by the extracts. Placental storage for three months at –20°C decreased anti-inflammatory activity of its extracts, and led to its complete loss during 6 months. Placental cryopreservation at –196°C enabled to preserve the anti-inflammatory and wound-healing activity. The cryopreserved placenta-derived extracts showed a pronounced positive effect on reparative process at third-degree (B) skin burn injury in rats.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49295426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of Low-Temperature Modes of Winter Rye Seed Storage on Its Yield-Related Traits 冬黑麦种子低温贮藏方式对产量性状的影响
Problems of Cryobiology and Cryomedicine Pub Date : 2022-06-30 DOI: 10.15407/cryo32.02.111
O. Zadorozhna, Dmytro Yehorov
{"title":"Influence of Low-Temperature Modes of Winter Rye Seed Storage on Its Yield-Related Traits","authors":"O. Zadorozhna, Dmytro Yehorov","doi":"10.15407/cryo32.02.111","DOIUrl":"https://doi.org/10.15407/cryo32.02.111","url":null,"abstract":"The effect of different storage modes for the seeds of winter rye breeidng sample (Secale cereale L. subsp. cereale var. vulgare Koern) on its longevity, germination and yield structure was studied. Seed samples with a moisture content of 5–7% were stored in an airtight container for up to 42 months at 4 and –20°C. Control of plant germination and assessment of yield-related traits were carried out for three years according to standard methods. The research findings demonstrated the variation of seed germination depending on the sample genotype. No differences were noted in the longevity of seeds after 30 and 42 months of storage under various temperature regimens. The results of the analysis of yield-related traits of plants for two or three consecutive years did not show any significant advantages when influenced by 4 or –20°C on the spike length, number of productive stems and the mass of grain derived from the spike. However, at the same time, differences were found for plant height and weight of 1,000 grains in some cases in the seeds stored at temperatures of 4 or −20°C.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44698949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cardiovascular System Response to Cold Stress in Individuals with Different Levels of Blood Pressure 不同血压水平个体的心血管系统对冷应激的反应
Problems of Cryobiology and Cryomedicine Pub Date : 2022-06-30 DOI: 10.15407/cryo32.02.134
S. Vadziuk, P. Tabas, Vitaliy Kulchytskyi, Ihor Pankiv
{"title":"Cardiovascular System Response to Cold Stress in Individuals with Different Levels of Blood Pressure","authors":"S. Vadziuk, P. Tabas, Vitaliy Kulchytskyi, Ihor Pankiv","doi":"10.15407/cryo32.02.134","DOIUrl":"https://doi.org/10.15407/cryo32.02.134","url":null,"abstract":"The study determines the peculiarities of the response of the central and peripheral links of to a cold stimulus in people with different levels of blood pressure (BP). The dynamics of blood pressure, heart rate, rheographic index and cardiorhythmography before and after the cold pressor test were evaluated. A significantly higher increase in systolic and diastolic BP in response to the cold pressor test was found in the persons with a normal high level of initial blood pressure (130/85–139/89 mm Hg) (p < 0.05). During the examination, a higher percentage of those with a hyperreactive blood pressure response was found (25%). In the experiment participants with initial blood pressure > 130/85 mm Hg a significant influence of the sympathetic nervous system on the cardiovascular system central and peripheral links in response to cold stress was observed. The individuals with normal (120/80– 129/84 mm Hg) and normal low (110/70-120/80 mm Hg) blood pressure showed a satisfactory response of the cardiovascular system to cold stress, so they should expect less health risks when working at low ambient temperatures.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42950924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Osmotic Characteristics of Erythrocytes After Freezing with Composition-Modified Cryopreservatives 复合改性冷冻防腐剂对红细胞冷冻后的渗透特性
Problems of Cryobiology and Cryomedicine Pub Date : 2022-06-30 DOI: 10.15407/cryo32.02.104
V. Ramazanov, Yelyzaveta Volovelska, V. Bondarenko
{"title":"Osmotic Characteristics of Erythrocytes After Freezing with Composition-Modified Cryopreservatives","authors":"V. Ramazanov, Yelyzaveta Volovelska, V. Bondarenko","doi":"10.15407/cryo32.02.104","DOIUrl":"https://doi.org/10.15407/cryo32.02.104","url":null,"abstract":"Here, we have studied the osmotic parameters of erythrocytes frozen in the composition-modified media with glycerol and 1,2-propanediol. A decreased glycerol concentration in a cryopreservative and an increased content of impermeable protective component sorbitol were established to affect the erythrocyte characteristics at different stages of freezing and hypothermic storage. Reduction of 1,2-propanediol and NaCl concentrations when augmenting the sucrose content in cryopreservative agent enabled changing a two-stage mode of erythrocyte freeze-thawing to the single-stage one. The presented here modification of cryoprotective medium allowed to diminish the level of damage to erythrocytes during freeze-thawing-washing out, that might prevent the inflammation development in a body when transfusing these cells.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42986783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Cryopreserved Human Cord Blood on Leukocyte Indices in Rats 人脐血冷冻保存对大鼠白细胞指标的影响
Problems of Cryobiology and Cryomedicine Pub Date : 2022-03-30 DOI: 10.15407/cryo32.01.058
V. Lomako, L. Pirozhenko
{"title":"Effect of Cryopreserved Human Cord Blood on Leukocyte Indices in Rats","authors":"V. Lomako, L. Pirozhenko","doi":"10.15407/cryo32.01.058","DOIUrl":"https://doi.org/10.15407/cryo32.01.058","url":null,"abstract":"","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49547701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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