胎盘低温贮藏对其提取物抗炎和创伤愈合性能的影响

Q4 Medicine

Problems of Cryobiology and Cryomedicine Pub Date : 2022-06-30 DOI:10.15407/cryo32.02.144

O. Bobrova, Ye. V. V. Naumenko, Myroslav Shchetinskyi, S. Narozhnyi, O. Nardid, М. Kalashnykova, Irina Shchetinska

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引用次数: 0

摘要

在红细胞热溶血和腺苷5'-二磷酸诱导的血小板聚集的细胞模型中，我们研究了胎盘在不同温度下的低温储存对其提取物抗炎活性的影响。在大鼠热烧伤模型中也评估了冷冻保存的胎盘提取物的伤口愈合效果。当胎盘保存长达一个月时，储存温度对提取物抑制红细胞热溶血和血小板聚集的百分比没有显著影响。胎盘在–20°C下储存三个月会降低其提取物的抗炎活性，并导致其在6个月内完全丧失。-196°C的胎盘冷冻保存能够保持抗炎和伤口愈合活性。冷冻保存的胎盘提取物对大鼠三度（B）皮肤烧伤的修复过程具有显著的积极作用。

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Low-Temperature Storage of Placenta Affects Anti-Inflammatory and Wound Healing Properties of Its Extracts

Here we have studied the effect of low-temperature storage of the placenta at different temperatures on anti-inflammatory activity of its extracts in cell models of erythrocyte thermohemolysis and adenosine-5’-diphosphate-induced platelet aggregation. The wound-healing effect of cryopreserved placental extracts was also evaluated in rat thermal burn model. When preserving placenta for up to a month, the temperature of storage was shown to cause no significant impact on the percentage of inhibition of erythrocyte thermohemolysis and platelet aggregation by the extracts. Placental storage for three months at –20°C decreased anti-inflammatory activity of its extracts, and led to its complete loss during 6 months. Placental cryopreservation at –196°C enabled to preserve the anti-inflammatory and wound-healing activity. The cryopreserved placenta-derived extracts showed a pronounced positive effect on reparative process at third-degree (B) skin burn injury in rats.

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来源期刊

Problems of Cryobiology and Cryomedicine Medicine-Medicine (miscellaneous)

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine. The journal covers all topics related to low temperature biology, medicine and engineering. These include but are not limited to: low temperature storage of biologicals (human, animal or plant cells, tissues, and organs), including preparation for storage, thawing/warming, cell and tissue culturing etc. response of biologicals to low temperature; cold adaptation of animals and plants; utilisation of low temperature in medicine; experimental and clinical transplantation, cell and tissue based therapies; developing of cryobiological and cryomedical devices; organisation and functioning of low temperature banks etc.