Problems of Cryobiology and Cryomedicine最新文献_第6页

Human mesenchymal stromal cells alginate encapsulation beneficial effect for storage at ambient temperature under hypoxic conditions 人间充质间质细胞海藻酸盐包封对常温低氧条件下的储存有益

Problems of Cryobiology and Cryomedicine Pub Date : 2022-12-30 DOI: 10.15407/cryo32.04.296a

Oleksandra Hubenia, Yuriy Kot, N. Trufanova

引用次数: 0

Cell-to-Cell Adhesion and Electrical Parameters of Murine Embryo Membranes After Cryopreservation by Vitrification 小鼠胚胎膜玻璃化冷冻保存后的细胞间粘附和电参数

Problems of Cryobiology and Cryomedicine Pub Date : 2022-12-30 DOI: 10.15407/cryo32.04.256

Y. Smolyaninova, V. Shigimaga, Lyudmila Popivnenko, I. Kovalenko

{"title":"Cell-to-Cell Adhesion and Electrical Parameters of Murine Embryo Membranes After Cryopreservation by Vitrification","authors":"Y. Smolyaninova, V. Shigimaga, Lyudmila Popivnenko, I. Kovalenko","doi":"10.15407/cryo32.04.256","DOIUrl":"https://doi.org/10.15407/cryo32.04.256","url":null,"abstract":"In this work, we have studied the impact of various stages of cryopreservation by vitrification in ethylene glycolsucrose medium on plasma membranes adhesive properties of 8-cell murine embryos and their specific electrical conductivity. Embryos were divided into the following experimental groups: the control, group 1 (incubation in vitrification medium) and group 2 (complete cycle of cryopreservation). The embryo exposure to vitrification medium did not affect their ability to cell-to-cell adhesion. After a complete cycle of cryopreservation, no embryo adhesion was observed. Electrical conductivity of embryo membranes was determined using the pulsed conductometry. After incubation in vitrification medium, their resistance to electric pulse was shown to decrease, that was manifested in the phenomenon of irreversible electric breakdown. The average values of electrical conductivity varied within the following ranges: ((12.1 ± 1.5)...(55.5 ± 2.6)), ((28.7 ± 5.7)...(44.± 8.9)), ((31.0 ± 9.3)...(87.9 ± 26.1)) μS/cm in the control, groups 1 and 2, respectively. These findings may be explained by appearance of first structural disorders in lipid bilayer of embryo membranes even at the stage of their incubation in vitrification medium. The lack of adhesive properties of blastomeres after vitrification-warming testified to a damage to the membrane adhesion proteins.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43140651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristic of migration in culture of newborn and fetal rat neural cells 新生和胎鼠神经细胞培养中的迁移特性

Problems of Cryobiology and Cryomedicine Pub Date : 2022-12-30 DOI: 10.15407/cryo32.04.306

S. Vsevolodska, O. Sukach, O. Ochenashko

引用次数: 0

Influence of the Me2SO removal on key parameters of cryopreserved autologous peripheral progenitor cells concentrates: a retrospective study in patients with primary or secondary amyloidosis Me2SO去除对冷冻保存的自体外周祖细胞浓缩物关键参数的影响:原发性或继发性淀粉样变性患者的回顾性研究

Problems of Cryobiology and Cryomedicine Pub Date : 2022-12-30 DOI: 10.15407/cryo32.04.298a

Miroslava Jandová, P. Měřička, J. Gregor, M. Lánská, D. Vokurková, I. Fátorová, A. Bezrouk, J. Radocha

引用次数: 0

Neurons of the olfactory neurogenic region of the rat: a morphological study 大鼠嗅觉神经源区神经元的形态学研究

Problems of Cryobiology and Cryomedicine Pub Date : 2022-12-30 DOI: 10.15407/cryo32.04.309

Alexandra Popovičová, K. Fabianová, M. Martončíková, A. Raček, E. Račeková

引用次数: 0

Cardioprotective Effect of Low Molecular Weight (Below 5 kDa) Fraction of Cattle Cord Blood Cryohemolysate 牛脐血冷冻溶血液低分子量（5kDa以下）组分的心脏保护作用

Problems of Cryobiology and Cryomedicine Pub Date : 2022-09-30 DOI: 10.15407/cryo32.03.206

I. Shcheniavsky, I. Kovalenko, O. Gulevsky

引用次数: 0

Medical and Psychological Rehabilitation for Patients with Spinal Degenerative Joint Diseases Using the Whole-Body Extreme Air Cryotherapy 全身极端空气冷冻治疗脊柱退行性关节疾病患者的医学和心理康复

Problems of Cryobiology and Cryomedicine Pub Date : 2022-09-30 DOI: 10.15407/cryo32.03.226

O. Panchenko, A. Kabantseva

{"title":"Medical and Psychological Rehabilitation for Patients with Spinal Degenerative Joint Diseases Using the Whole-Body Extreme Air Cryotherapy","authors":"O. Panchenko, A. Kabantseva","doi":"10.15407/cryo32.03.226","DOIUrl":"https://doi.org/10.15407/cryo32.03.226","url":null,"abstract":"Here, we have studied the rehabilitation efficiency for patients with spinal degenerative joint diseases using the wholebody extreme air cryotherapy (WBEACT) according to our own technique. The assessment of pain intensity on visual-analogue scale upon completing the rehabilitation therapy showed the pain disappearance in 68.4% of patients, and its significant reduction in 31.6%; increased daily motor activity, and decreased restrictions during walking in 90.8% of cases; improved well-being in 35.7%; enhanced activity in 43.9%, and mood change in 38.8%. Each WBEACT session increased the muscle strength and endurance for both genders of patients, thus testifying to a stimulating effect of cryotherapy to human body. The WBEACT application for rehabilitation of patients with spinal degenerative joint diseases enabled to normalize the psycho-emotional state as quickly as possible and improve functional state of neuromuscular apparatus, i. e. to reduce the pain and muscle tonic syndromes, strengthen the muscular skeleton, and increase the range of motion and endurance while standing, sitting and walking.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48828953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physical Factors of Beetroot Seed Presowing Treatment Affect Sowing Quality and Crop Yield 甜菜种子预处理的物理因素对播种质量和产量的影响

Problems of Cryobiology and Cryomedicine Pub Date : 2022-09-30 DOI: 10.15407/cryo32.03.183

Nadiia Shevchenko, G. Kovalenko, N. Bashtan, A. Mozgovska, Tatiana Miroshnichenko, T. Strybul, T. Ivchenko, O. Kuts

{"title":"Physical Factors of Beetroot Seed Presowing Treatment Affect Sowing Quality and Crop Yield","authors":"Nadiia Shevchenko, G. Kovalenko, N. Bashtan, A. Mozgovska, Tatiana Miroshnichenko, T. Strybul, T. Ivchenko, O. Kuts","doi":"10.15407/cryo32.03.183","DOIUrl":"https://doi.org/10.15407/cryo32.03.183","url":null,"abstract":"In this work, we have studied the impact of ozonation, freezing down to liquid nitrogen temperature and their combination on sowing quality and crop yield for Dii cultivar beetroot seeds. The optimal moisture, ozone dose within the ozone-air mixture, and the exposure time, not reducing the germination rate, were determined. The ozonation, freezing, and their combined effect affected neither the seed germination in laboratory, nor biometric indices in a field. The seed freezing down to liquid nitrogen temperature was found to significantly increase its crop yield. Biochemical analysis of root crops showed all the ways of presowing treatment to reduce the content of total sugars and betanine. After freezing and ozonation, the amount of dry matter decreased, but after combined exposure it augmented. Ozonation and subsequent freezing lowered the content of ascorbic acid. Significantly reduced studied parameters did not reach the minimum values specific to this crop. Freezing and a combined impact of the studied factors on the seeds increased the nitrate content in root crops, which did not exceed the maximum allowable rate.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45153331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sperm DNA Cryodamage in Domestic and Farm Animals: Detection Methods and Ways of DNA Integrity Improvement 家畜和农场动物精子DNA低温损伤:DNA完整性改善的检测方法和途径

Problems of Cryobiology and Cryomedicine Pub Date : 2022-09-30 DOI: 10.15407/cryo32.03.171

T. Yurchuk, O. Pavlovych, M. Petrushko

引用次数: 0

Morphogenetic Responses of Triticum aestivum L. Calus Culture During Long-Term Effect of Low Positive Temperatures 低正温度长期作用下小麦Calus培养的形态发生反应

Problems of Cryobiology and Cryomedicine Pub Date : 2022-09-30 DOI: 10.15407/cryo32.03.214

O. Avksentieva, V. Zhmurko, Viktoriia Chumakova

引用次数: 0