Problems of Cryobiology and Cryomedicine最新文献

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Lyophilized Human Cord Blood Leukoconcentrate Affects Immune System of Rats with Ischemic Stroke 冻干人脐带血浓缩物对缺血性中风大鼠免疫系统的影响
Problems of Cryobiology and Cryomedicine Pub Date : 2022-12-30 DOI: 10.15407/cryo32.04.277
V. Lebedynets, Ludmila Ostankova, M. Bondarovych, D. Lebedynets, M. Ostankov, O. Lutsenko, Igor Grisha, A. Goltsev
{"title":"Lyophilized Human Cord Blood Leukoconcentrate Affects Immune System of Rats with Ischemic Stroke","authors":"V. Lebedynets, Ludmila Ostankova, M. Bondarovych, D. Lebedynets, M. Ostankov, O. Lutsenko, Igor Grisha, A. Goltsev","doi":"10.15407/cryo32.04.277","DOIUrl":"https://doi.org/10.15407/cryo32.04.277","url":null,"abstract":"Cerebrovascular pathology, especially acute cerebrovascular accident is among the most complicated issues in medicine. Therefore, the search for efficient ways to treat neurodegenerative diseases has still remained topical. In experimental model of ischemic stroke (IS), the impact of lyophilized and cryopreserved human cord blood leukoconcentrate (lHCBL and cHCBL, respectively) on immune system humoral and cell components has been comparatively studied. The ischemic stroke was simulated in 6-monthold Wistar rats by occlusion of the middle cerebral artery. The lHCBL and cHCBL were administered intraperitoneally at a dose of 0.5 ml of 5 × 106 cells in 6 hrs after surgery (occlusion). In blood serum, the populations and subpopulations of lymphocytes (CD3+, CD4+, CD8+, CD16+, CD4+CD25+), the content of total immunoglobulins and circulating immune complexes were determined. The experimental model of IS showed both lyophilization and cryopreservation of HCBL to ensure the preservation of immunomodulatory potential of cells, that was confirmed in vivo. An equivalent corrective effect of lHCBL and cHCBL on indices of immune system cell and humoral components during IS development has been established. The inclusion of lHCBL, as well as cHCBL and native HCBL in basic therapy of IS promoted a more rapid recovery of the immune system indices in animals.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49164099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Establishing key factors for successful mussel biobanking 建立贻贝生物库成功的关键因素
Problems of Cryobiology and Cryomedicine Pub Date : 2022-12-30 DOI: 10.15407/cryo32.04.299a
Sara Campos Rosende, D. Costas, J. Troncoso, E. Paredes
{"title":"Establishing key factors for successful mussel biobanking","authors":"Sara Campos Rosende, D. Costas, J. Troncoso, E. Paredes","doi":"10.15407/cryo32.04.299a","DOIUrl":"https://doi.org/10.15407/cryo32.04.299a","url":null,"abstract":"","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42891494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of cryopreserved multipotent mesenchymal stromal cells on regulation of immune inflammation in adjuvant arthritis 冷冻保存的多能间充质基质细胞对佐剂性关节炎免疫炎症调节的影响
Problems of Cryobiology and Cryomedicine Pub Date : 2022-12-30 DOI: 10.15407/cryo32.04.305a
D. Vvedenskyi, N. Volkova, M. Yukhta, A. Goltsev
{"title":"Influence of cryopreserved multipotent mesenchymal stromal cells on regulation of immune inflammation in adjuvant arthritis","authors":"D. Vvedenskyi, N. Volkova, M. Yukhta, A. Goltsev","doi":"10.15407/cryo32.04.305a","DOIUrl":"https://doi.org/10.15407/cryo32.04.305a","url":null,"abstract":"","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46100757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Natural deep eutectic systems: efficient alternative cryoprotectant agents for the cryopreservation of cells 天然深共晶体系:用于细胞冷冻保存的有效替代冷冻保护剂
Problems of Cryobiology and Cryomedicine Pub Date : 2022-12-30 DOI: 10.15407/cryo32.04.298
A. R. Jesus, Alexandre Paiva, A. Duarte
{"title":"Natural deep eutectic systems: efficient alternative cryoprotectant agents for the cryopreservation of cells","authors":"A. R. Jesus, Alexandre Paiva, A. Duarte","doi":"10.15407/cryo32.04.298","DOIUrl":"https://doi.org/10.15407/cryo32.04.298","url":null,"abstract":"","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46328488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Search for methodological approaches of organotypic cultivation for following cryopreservation 为后续冷冻保存寻找器官型培养的方法学方法
Problems of Cryobiology and Cryomedicine Pub Date : 2022-12-30 DOI: 10.15407/cryo32.04.307
Аnna Kaverinska, A. Tkachenko, Volodymyr Prokopiuk
{"title":"Search for methodological approaches of organotypic cultivation for following cryopreservation","authors":"Аnna Kaverinska, A. Tkachenko, Volodymyr Prokopiuk","doi":"10.15407/cryo32.04.307","DOIUrl":"https://doi.org/10.15407/cryo32.04.307","url":null,"abstract":"","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47952614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improvement of scaffold cryopreservation by use of magnetic nanoparticles 磁性纳米粒子对支架冷冻保存的改进
Problems of Cryobiology and Cryomedicine Pub Date : 2022-12-30 DOI: 10.15407/cryo32.04.303
S. Leal-Marin, Kai Höltje, A. Lopera, C. Garcia, B. Glasmacher, O. Gryshkov
{"title":"Improvement of scaffold cryopreservation by use of magnetic nanoparticles","authors":"S. Leal-Marin, Kai Höltje, A. Lopera, C. Garcia, B. Glasmacher, O. Gryshkov","doi":"10.15407/cryo32.04.303","DOIUrl":"https://doi.org/10.15407/cryo32.04.303","url":null,"abstract":"","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44880861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antagonistic activity of immobilized symbiotics after lyophilization and storage at different temperatures 固定化共生体在冻干和不同温度下的拮抗活性
Problems of Cryobiology and Cryomedicine Pub Date : 2022-12-30 DOI: 10.15407/cryo32.04.308a
Halyna Shkarlat, Ya.O. Cherkashyna, L. Stepanyuk, Stanislav Sevastianov
{"title":"Antagonistic activity of immobilized symbiotics after lyophilization and storage at different temperatures","authors":"Halyna Shkarlat, Ya.O. Cherkashyna, L. Stepanyuk, Stanislav Sevastianov","doi":"10.15407/cryo32.04.308a","DOIUrl":"https://doi.org/10.15407/cryo32.04.308a","url":null,"abstract":"","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45060398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of placental tissue freeze-thawing modes on composition and biological activity of its cryoextracts 胎盘组织冻融方式对其冷冻提取物组成及生物活性的影响
Problems of Cryobiology and Cryomedicine Pub Date : 2022-12-30 DOI: 10.15407/cryo32.04.308
Yu.V. Chyzh, L. Marchenko, T. Govorukha, M. Repin
{"title":"Effect of placental tissue freeze-thawing modes on composition and biological activity of its cryoextracts","authors":"Yu.V. Chyzh, L. Marchenko, T. Govorukha, M. Repin","doi":"10.15407/cryo32.04.308","DOIUrl":"https://doi.org/10.15407/cryo32.04.308","url":null,"abstract":"","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43090608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Blood Hypothermic Storage and Erythrocyte Cryopreservation in Dogs 狗的血液低温储存和红细胞低温保存
Problems of Cryobiology and Cryomedicine Pub Date : 2022-12-30 DOI: 10.15407/cryo32.04.245
G. Zhegunov, O. Denysova, Galina Zhegunova
{"title":"Blood Hypothermic Storage and Erythrocyte Cryopreservation in Dogs","authors":"G. Zhegunov, O. Denysova, Galina Zhegunova","doi":"10.15407/cryo32.04.245","DOIUrl":"https://doi.org/10.15407/cryo32.04.245","url":null,"abstract":"This review presents the analysis of reported data on the current methods for blood hypothermic storage and erythrocyte cryopreservation in dogs. The relevance of designing and improving the techniques for canine erythrocyte cryopreservation, cryobanking, as well as the application of cryopreserved blood components for transfusion were substantiated. The features of canine blood hypothermic storage and specific use of cryoprotectants with various modes of their action were considered. The advantages of applying DMSO and combined cryopreservatives during low temperature storage of canine erythrocytes were specified.","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45799341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Toxicity of cryoprotectants to Chlorococcum dissectum microalgae cells 低温保护剂对解剖绿球微藻细胞的毒性研究
Problems of Cryobiology and Cryomedicine Pub Date : 2022-12-30 DOI: 10.15407/cryo32.04.304a
K. Vozovyk, N. Chernobai, N. Kadnikova, L. Rozanov
{"title":"Toxicity of cryoprotectants to Chlorococcum dissectum microalgae cells","authors":"K. Vozovyk, N. Chernobai, N. Kadnikova, L. Rozanov","doi":"10.15407/cryo32.04.304a","DOIUrl":"https://doi.org/10.15407/cryo32.04.304a","url":null,"abstract":"","PeriodicalId":53457,"journal":{"name":"Problems of Cryobiology and Cryomedicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47822978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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