Lyophilized Human Cord Blood Leukoconcentrate Affects Immune System of Rats with Ischemic Stroke

Q4 Medicine

Problems of Cryobiology and Cryomedicine Pub Date : 2022-12-30 DOI:10.15407/cryo32.04.277

V. Lebedynets, Ludmila Ostankova, M. Bondarovych, D. Lebedynets, M. Ostankov, O. Lutsenko, Igor Grisha, A. Goltsev

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引用次数: 1

Abstract

Cerebrovascular pathology, especially acute cerebrovascular accident is among the most complicated issues in medicine. Therefore, the search for efficient ways to treat neurodegenerative diseases has still remained topical. In experimental model of ischemic stroke (IS), the impact of lyophilized and cryopreserved human cord blood leukoconcentrate (lHCBL and cHCBL, respectively) on immune system humoral and cell components has been comparatively studied. The ischemic stroke was simulated in 6-monthold Wistar rats by occlusion of the middle cerebral artery. The lHCBL and cHCBL were administered intraperitoneally at a dose of 0.5 ml of 5 × 106 cells in 6 hrs after surgery (occlusion). In blood serum, the populations and subpopulations of lymphocytes (CD3+, CD4+, CD8+, CD16+, CD4+CD25+), the content of total immunoglobulins and circulating immune complexes were determined. The experimental model of IS showed both lyophilization and cryopreservation of HCBL to ensure the preservation of immunomodulatory potential of cells, that was confirmed in vivo. An equivalent corrective effect of lHCBL and cHCBL on indices of immune system cell and humoral components during IS development has been established. The inclusion of lHCBL, as well as cHCBL and native HCBL in basic therapy of IS promoted a more rapid recovery of the immune system indices in animals.

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冻干人脐带血浓缩物对缺血性中风大鼠免疫系统的影响

脑血管病理学，特别是急性脑血管意外是医学上最复杂的问题之一。因此，寻找治疗神经退行性疾病的有效方法仍然是一个热门话题。在缺血性脑卒中（IS）实验模型中，比较研究了冻干和冷冻保存的人脐血白细胞浓缩物（分别为lHCBL和cHCBL）对免疫系统体液和细胞成分的影响。通过阻断大脑中动脉，在6个月大的Wistar大鼠中模拟缺血性卒中。lHCBL和cHCBL在手术后6小时内腹膜内给药，剂量为0.5 ml的5×106个细胞（闭塞）。在血清中，测定淋巴细胞的群体和亚群（CD3+、CD4+、CD8+、CD16+、CD4+CD25+）、总免疫球蛋白和循环免疫复合物的含量。IS的实验模型显示了HCBL的冷冻干燥和冷冻保存，以确保细胞免疫调节潜力的保存，这在体内得到了证实。已经建立了lHCBL和cHCBL对IS发育过程中免疫系统细胞和体液成分指标的等效校正作用。在IS的基础治疗中加入lHCBL、cHCBL和天然HCBL促进了动物免疫系统指数的更快恢复。

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来源期刊

Problems of Cryobiology and Cryomedicine Medicine-Medicine (miscellaneous)

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine. The journal covers all topics related to low temperature biology, medicine and engineering. These include but are not limited to: low temperature storage of biologicals (human, animal or plant cells, tissues, and organs), including preparation for storage, thawing/warming, cell and tissue culturing etc. response of biologicals to low temperature; cold adaptation of animals and plants; utilisation of low temperature in medicine; experimental and clinical transplantation, cell and tissue based therapies; developing of cryobiological and cryomedical devices; organisation and functioning of low temperature banks etc.