复合改性冷冻防腐剂对红细胞冷冻后的渗透特性

Q4 Medicine

Problems of Cryobiology and Cryomedicine Pub Date : 2022-06-30 DOI:10.15407/cryo32.02.104

V. Ramazanov, Yelyzaveta Volovelska, V. Bondarenko

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引用次数: 0

摘要

在这里，我们研究了用甘油和1,2-丙二醇组成的改性介质冷冻红细胞的渗透参数。在冷冻和低温储存的不同阶段，冷冻保存剂中甘油浓度的降低和不可渗透保护成分山梨醇含量的增加影响了红细胞的特性。当增加冷冻保存剂中的蔗糖含量时，降低1,2-丙二醇和NaCl的浓度，使红细胞冻融的两阶段模式变为单阶段模式。本文提出的冷冻保护介质的修饰可以降低冻融冲洗过程中对红细胞的损伤水平，这可能会防止在输注这些细胞时体内炎症的发展。

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Osmotic Characteristics of Erythrocytes After Freezing with Composition-Modified Cryopreservatives

Here, we have studied the osmotic parameters of erythrocytes frozen in the composition-modified media with glycerol and 1,2-propanediol. A decreased glycerol concentration in a cryopreservative and an increased content of impermeable protective component sorbitol were established to affect the erythrocyte characteristics at different stages of freezing and hypothermic storage. Reduction of 1,2-propanediol and NaCl concentrations when augmenting the sucrose content in cryopreservative agent enabled changing a two-stage mode of erythrocyte freeze-thawing to the single-stage one. The presented here modification of cryoprotective medium allowed to diminish the level of damage to erythrocytes during freeze-thawing-washing out, that might prevent the inflammation development in a body when transfusing these cells.

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来源期刊

Problems of Cryobiology and Cryomedicine Medicine-Medicine (miscellaneous)

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine. The journal covers all topics related to low temperature biology, medicine and engineering. These include but are not limited to: low temperature storage of biologicals (human, animal or plant cells, tissues, and organs), including preparation for storage, thawing/warming, cell and tissue culturing etc. response of biologicals to low temperature; cold adaptation of animals and plants; utilisation of low temperature in medicine; experimental and clinical transplantation, cell and tissue based therapies; developing of cryobiological and cryomedical devices; organisation and functioning of low temperature banks etc.