Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis最新文献_第4页

Genotoxicity of nanoparticles evaluated using the in vitro micronucleus assay, a review of recent data^§. 使用体外微核试验评估纳米颗粒的遗传毒性，最近数据的回顾§。

IF 2.3 4区环境科学与生态学

Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis Pub Date : 2025-01-01 Epub Date: 2025-05-17 DOI: 10.1080/26896583.2025.2503646

Alexander W Alund, Li Xia, Tao Chen

{"title":"Genotoxicity of nanoparticles evaluated using the in vitro micronucleus assay, a review of recent data§.","authors":"Alexander W Alund, Li Xia, Tao Chen","doi":"10.1080/26896583.2025.2503646","DOIUrl":"10.1080/26896583.2025.2503646","url":null,"abstract":"The in vitro micronucleus assay is a well-known and established component of the standard genotoxicity test battery. The growing use of nanomaterials around the world along with human exposure to them has increased the need for risk assessment with regard to safety, including potential genotoxicity. The in vitro micronucleus assay is one of the most used tests for evaluating the genotoxicity of nanomaterials. This review compiles studies since 2017 that performed assessments of micronucleus formation in vitro after cellular exposure to different nanomaterials. Genotoxicity of a broad range of nanomaterials including silver, cerium, zinc, gold, nickel, cadmium, titanium, carbon, and aluminum in different cell types were reviewed. While clear trends could be seen for some nanoparticle types like silver and cerium nanoparticles, others like gold nanoparticles showed mixed results. This review highlights the usefulness and effectiveness of the micronucleus assay for studying the genotoxicity of nanomaterials, in part, and is also careful to note that standard guidelines should be followed when conducting this assay in order to generate reliable and quality-driven data.","PeriodicalId":53200,"journal":{"name":"Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis","volume":" ","pages":"209-227"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12439229/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144095804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of HepaRG cells for genotoxicity assessment: a review. 应用 HepaRG 细胞进行遗传毒性评估：综述。

IF 2.3 4区环境科学与生态学

Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis Pub Date : 2024-01-01 Epub Date: 2024-04-02 DOI: 10.1080/26896583.2024.2331956

Xiaoqing Guo, Hannah Xu, Ji-Eun Seo

{"title":"Application of HepaRG cells for genotoxicity assessment: a review.","authors":"Xiaoqing Guo, Hannah Xu, Ji-Eun Seo","doi":"10.1080/26896583.2024.2331956","DOIUrl":"10.1080/26896583.2024.2331956","url":null,"abstract":"There has been growing interest in the use of human-derived metabolically competent cells for genotoxicity testing. The HepaRG cell line is considered one of the most promising cell models because it is TP53-proficient and retains many characteristics of primary human hepatocytes. In recent years, HepaRG cells, cultured in both a traditional two-dimensional (2D) format and as more advanced in-vivo-like 3D spheroids, have been employed in assays that measure different types of genetic toxicity endpoints, including DNA damage, mutations, and chromosomal damage. This review summarizes published studies that have used HepaRG cells for genotoxicity assessment, including cell model evaluation studies and risk assessment for various compounds. Both 2D and 3D HepaRG models can be adapted to several high-throughput genotoxicity assays, generating a large number of data points that facilitate quantitative benchmark concentration modeling. With further validation, HepaRG cells could serve as a unique, human-based new alternative methodology for in vitro genotoxicity testing.","PeriodicalId":53200,"journal":{"name":"Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis","volume":" ","pages":"214-237"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12395423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140875024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolism and liver toxicity of cannabidiol. 大麻二酚的代谢和肝毒性。

IF 1.2 4区环境科学与生态学

Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis Pub Date : 2024-01-01 Epub Date: 2024-06-21 DOI: 10.1080/26896583.2024.2366741

Si Chen, Yuxi Li, Xilin Li, Qiangen Wu, Montserrat Puig, Frederic Moulin, Jeremy Gingrich, Lei Guo

{"title":"Metabolism and liver toxicity of cannabidiol.","authors":"Si Chen, Yuxi Li, Xilin Li, Qiangen Wu, Montserrat Puig, Frederic Moulin, Jeremy Gingrich, Lei Guo","doi":"10.1080/26896583.2024.2366741","DOIUrl":"10.1080/26896583.2024.2366741","url":null,"abstract":"Increasing public interest has resulted in the widespread use of non-pharmaceutical cannabidiol (CBD) products. The sales of CBD products continue to rise, accompanied by concerns regarding unsubstantiated benefits, lack of product quality control, and potential health risks. Both animal and human studies have revealed a spectrum of toxicological effects linked to the use of CBD. Adverse effects related to exposure of humans to CBD include changes in appetite, gastrointestinal discomfort, fatigue, and elevated liver aminotransferase enzymes. Animal studies reported changes in organ weight, reproduction, liver function, and the immune system. This review centers on human-derived data, including clinical studies and in vitro investigations. Animal studies are also included when human data is not available. The objective is to offer an overview of CBD-related hepatotoxicity, metabolism, and potential CBD-drug interactions, thereby providing insights into the current understanding of CBD's impact on human health. It's important to note that this review does not serve as a risk assessment but seeks to summarize available information to contribute to the broader understanding of potential toxicological effects of CBD on the liver.","PeriodicalId":53200,"journal":{"name":"Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis","volume":" ","pages":"238-254"},"PeriodicalIF":1.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11404724/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141433349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of antimicrobial and antioxidant activity of zinc oxide nanoparticles biosynthesized with Ziziphus spina-christi leaf extracts. 评估用刺五加叶提取物生物合成的纳米氧化锌的抗菌和抗氧化活性。

IF 2.5 4区环境科学与生态学

Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis Pub Date : 2024-01-01 Epub Date: 2023-12-18 DOI: 10.1080/26896583.2023.2293443

Bushra H Shnawa, Parwin J Jalil, Ali Al-Ezzi, Renjbar M Mhamedsharif, Daniyal A Mohammed, Donia M Biro, Mukhtar H Ahmed

{"title":"Evaluation of antimicrobial and antioxidant activity of zinc oxide nanoparticles biosynthesized with Ziziphus spina-christi leaf extracts.","authors":"Bushra H Shnawa, Parwin J Jalil, Ali Al-Ezzi, Renjbar M Mhamedsharif, Daniyal A Mohammed, Donia M Biro, Mukhtar H Ahmed","doi":"10.1080/26896583.2023.2293443","DOIUrl":"10.1080/26896583.2023.2293443","url":null,"abstract":"Background: Due to their simplicity, eco-friendliness, availability and non-toxicity, the greener fabrication of metal and metal oxide nanoparticles has been a highly attractive research area over the last decade. Aim: This study aimed to assess the antioxidant and antimicrobial activities of the green synthesized zinc oxide nanoparticles (ZnO-NPs) using an aqueous leaf extract of Ziziphus spina-christi. Method: The antioxidant property of ZnO-NPs was analyzed by the α, α-diphenyl-β-picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2). Additionally, the diffusion agar method assessed the antimicrobial activities against bacteria and fungi. Results: ZnO-NPs synthesized by Z. spina-christi had shown promising H2O2 and DPPH free radical scavenging actions compared to vitamin C. The ZnO-NPs exhibited significant antibacterial activity against the tested bacteria with various susceptibility as a concentration-dependent effect. The largest zone of inhibition for Staphylococcus aureus (S. aureus) was observed (36 ± 2 mm) compared to Escherichia coli (E. coli) (15 ± 2 mm) by the same concentration of ZnO-NPs. The ZnO-NPs showed remarkable antifungal activity against Aspergillus niger. Conclusion: It can be concluded that, ZnO-NP have been imposed as suitable antimicrobial agent being able to combat both S. aureus and E. coli in vitro.","PeriodicalId":53200,"journal":{"name":"Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis","volume":" ","pages":"93-108"},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138799657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the fate of disinfection by-products in swimming pools: current empirical and mechanistic modeling insights. 了解游泳池中消毒副产品的归宿：当前的经验和机理模型见解。

IF 1.2 4区环境科学与生态学

Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis Pub Date : 2024-01-01 Epub Date: 2024-08-29 DOI: 10.1080/26896583.2024.2396250

Shaorong Chen, Zijian Li

{"title":"Understanding the fate of disinfection by-products in swimming pools: current empirical and mechanistic modeling insights.","authors":"Shaorong Chen, Zijian Li","doi":"10.1080/26896583.2024.2396250","DOIUrl":"10.1080/26896583.2024.2396250","url":null,"abstract":"Disinfecting swimming pool water plays a crucial role in preventing the spread of harmful bacteria. However, the interaction between disinfectants and precursors can lead to the formation of potentially disinfection by-products (DBPs). Prolonged exposure to these DBPs may pose health risks. This review study investigates recent research advancements concerning the formation, exposure, and regulation of DBPs within swimming pools. It also provides an overview of existing models that predict DBPs generation in pools, highlighting their limitations. The review explores the mechanisms behind DBPs formation under different disinfectant and precursor conditions. It specifically discusses two types of models that simulate the production of these by-products. Compared to drinking water, swimming pool water presents unique challenges for model development due to its complex mix of external substances, human activities, and environmental factors. Existing models can be categorized as empirical or mechanistic. Empirical models focus on water quality parameters and operational practices, while mechanistic models delve deeper into the kinetics of DBPs generation and the dynamic nature of these compounds. By employing these models, it becomes possible to minimize DBPs production, optimize equipment design, enhance operational efficiency, and manage mechanical ventilation systems effectively.","PeriodicalId":53200,"journal":{"name":"Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis","volume":" ","pages":"366-401"},"PeriodicalIF":1.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142114713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing the impact and mechanisms of environmental pollutants (heavy metals and pesticides) on the male reproductive system: a comprehensive review. 评估环境污染物（重金属和杀虫剂）对男性生殖系统的影响和机制：全面综述。

IF 2.5 4区环境科学与生态学

Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis Pub Date : 2024-01-01 Epub Date: 2024-01-19 DOI: 10.1080/26896583.2024.2302738

Rohit Gautam, Eepsita Priyadarshini, Arbind Kumar Patel, Taruna Arora

{"title":"Assessing the impact and mechanisms of environmental pollutants (heavy metals and pesticides) on the male reproductive system: a comprehensive review.","authors":"Rohit Gautam, Eepsita Priyadarshini, Arbind Kumar Patel, Taruna Arora","doi":"10.1080/26896583.2024.2302738","DOIUrl":"10.1080/26896583.2024.2302738","url":null,"abstract":"The escalation of technological advancements, coupled with the increased use of hazardous chemicals, has emerged as a significant concern for human health. Exposure to environmental pollutants like heavy metals and pesticides (insecticides, herbicides and fungicides) is known to significantly contribute to various health problems, particularly affecting reproductive health. Disturbances in reproductive potential and reproductive toxicity in males are particularly worrisome. Existing literature suggests that exposure to these environmental pollutants significantly alters male reproductive parameters. Thus, it is imperative to thoroughly analyze, comprehend, and evaluate their impact on male reproductive toxicity. Oxidative stress and disruptions in redox equilibrium are major factors through which these pollutants induce changes in sperm parameters and affect the reproductive system. Insecticides, fungicides, and herbicides act as endocrine disruptors, interfering with the secretion and function of reproductive hormones such as testosterone and luteinizing hormone (LH), consequently impacting spermatogenesis. Additionally, heavy metals are reported to bio-accumulate in reproductive organs, acting as endocrine disruptors and triggering oxidative stress. The co-operative association of these pollutants can lead to severe damage. In this comprehensive review, we have conducted an in-depth analysis of the impact of these environmental pollutants on the male reproductive system, shedding light on the underlying mechanisms of action.","PeriodicalId":53200,"journal":{"name":"Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis","volume":" ","pages":"126-153"},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139492962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 1.2 4区环境科学与生态学

Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis Pub Date : 2024-01-01 Epub Date: 2024-02-26 DOI: 10.1080/26896583.2024.2319010

Hyung-Min Ahn, Insu Park, Chang Geun Kim, Young Kyung Ko, Jeong-An Gim

{"title":"Factors related to tumor response rate from TCGA three omics data-variants, expression, methylation.","authors":"Hyung-Min Ahn, Insu Park, Chang Geun Kim, Young Kyung Ko, Jeong-An Gim","doi":"10.1080/26896583.2024.2319010","DOIUrl":"10.1080/26896583.2024.2319010","url":null,"abstract":"The Cancer Genome Atlas (TCGA) and its patient-derived multi-omics datasets have been the backbone of cancer research, and with novel approaches, it continues to shed new insight into the disease. In this study, we delved into a method of multi-omics integration of patient datasets and the association of biological pathways related to the disease. First, across thirty-three types of cancer present in TCGA, we merged genomic mutations and drug response datasets and filtered for the viable variant-drug response combinations available in TCGA, containing more than three samples for each drug response label with RNA sequencing (RNA-seq) and genomic methylation data available for each patient. We identified two distinct combinations in TCGA, one being pancreatic adenocarcinoma patients with/without rs121913529 variant in KRAS gene treated with gemcitabine, and the other low-grade glioma with/without rs121913500 variant in IDH1 gene administered with temozolomide. In these two groups, different patterns of gene expression were observed in the pathways often associated with cancer progression, such as mTOR and PDGF between the patients with complete response and progressive disease. Our result will provide yet another example of the relevance of these biological pathways in cancer drug response and a way for multi-omics integration in cancer datasets.","PeriodicalId":53200,"journal":{"name":"Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis","volume":" ","pages":"173-188"},"PeriodicalIF":1.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139974547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heavy metal and microbial testing of selected cosmetic products in the Palestinian market. 对巴勒斯坦市场上选定的化妆品进行重金属和微生物检测。

IF 2.5 4区环境科学与生态学

Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis Pub Date : 2024-01-01 Epub Date: 2024-01-19 DOI: 10.1080/26896583.2023.2281199

Murad Abualhasan, Liza Naffa, Ro'a Alarda, Baraa Zahi, Ameed Amireh, Munir Al-Atrash

{"title":"Heavy metal and microbial testing of selected cosmetic products in the Palestinian market.","authors":"Murad Abualhasan, Liza Naffa, Ro'a Alarda, Baraa Zahi, Ameed Amireh, Munir Al-Atrash","doi":"10.1080/26896583.2023.2281199","DOIUrl":"10.1080/26896583.2023.2281199","url":null,"abstract":"Excessive and continuous use of cosmetic products containing heavy metals can lead to harmful effects. International regulations mandate limited quantities of heavy metals contamination in cosmetic preparations to ensure consumer safety. This research aims to evaluate heavy metal and microbial contamination levels in selected cosmetic products available in the Palestinian market. We collected 35 samples randomly from 23 companies, representing four product types, and analyzed them for the presence of seven heavy metals: zinc (Zn), cadmium (Cd), lead (Pb), chromium (Cr), iron (Fe), copper (Cu), and arsenic (As) using an atomic absorption spectrometer. We also interviewed pharmacists who sold these cosmetics to assess their knowledge of allowed limits and toxic effects associated with increased heavy metal content in cosmetics. The results indicated that all tested products exceeded the allowed limit for Cd (9.5 ± 2.3 ppm), Cu (33.8 ± 9.2 ppm), and Zn (151.0 ± 7.4 ppm). However, none of the tested samples showed microbial contamination. These findings underscore the significant heavy metal contamination of cosmetics present in the Palestinian market. Thus, there is a pressing need to register and quality-test all cosmetic products sold in the Palestinian market and to raise the pharmacists' awareness and knowledge regarding heavy metals in cosmetics.","PeriodicalId":53200,"journal":{"name":"Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis","volume":" ","pages":"1-15"},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138500105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

More about making profits or providing safe drinking water? A state-of-the-art review on sachet water contamination in Nigeria. 盈利更重要还是提供安全饮用水更重要？尼日利亚袋装水污染现状综述。

IF 1.2 4区环境科学与生态学

Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis Pub Date : 2024-01-01 Epub Date: 2024-03-04 DOI: 10.1080/26896583.2024.2319009

Johnson C Agbasi, Arinze Longinus Ezugwu, Michael Ekuru Omeka, Ifeanyi Adolphus Ucheana, Chiedozie Chukwuemeka Aralu, Hillary Onyeka Abugu, Johnbosco C Egbueri

{"title":"More about making profits or providing safe drinking water? A state-of-the-art review on sachet water contamination in Nigeria.","authors":"Johnson C Agbasi, Arinze Longinus Ezugwu, Michael Ekuru Omeka, Ifeanyi Adolphus Ucheana, Chiedozie Chukwuemeka Aralu, Hillary Onyeka Abugu, Johnbosco C Egbueri","doi":"10.1080/26896583.2024.2319009","DOIUrl":"10.1080/26896583.2024.2319009","url":null,"abstract":"Public health concerns on surface and groundwater contamination worldwide have increased. Sachet water contamination has also raised serious concerns across many developing countries. While previous studies attempted to address this issue, this review takes a different approach by utilizing a comprehensive analysis of physicochemical parameters, heavy metals, and microbial loads tested in sachet water across Nigeria's six geopolitical zones, within the period of 2020-2023. In this review study, over 50 articles were carefully analyzed. Collected data unveiled regional variations in the quality of sachet water across Nigeria. Noteworthy concerns revolve around levels of pH, total hardness, magnesium, calcium, nickel, iron, lead, mercury, arsenic, and cadmium. Fecal contamination was also identified as a significant issue, with the prevalence of several pathogens like Escherichia coli, Salmonella typhi, Enterobacter cloacae, Staphylococcus aureus, and Enterococcus faecalis. The manufacturing, delivery, storage, and final sale of sachet water, as well as poor environmental hygiene, were identified as potential contamination sources. The intake of contaminated sachet water exposes the citizens to waterborne and carcinogenic diseases. While the sachet water industry keeps growing and making profits, it is apparent that improvement calls made by previous studies, regarding the quality of water produced, have not been paid serious attention.","PeriodicalId":53200,"journal":{"name":"Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis","volume":" ","pages":"255-297"},"PeriodicalIF":1.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140029584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular and molecular alterations in a human hepatocellular in vitro model of nonalcoholic fatty liver disease development and stratification. 非酒精性脂肪肝发展和分层的人类肝细胞体外模型中的细胞和分子变化。

IF 2.5 4区环境科学与生态学

Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis Pub Date : 2024-01-01 Epub Date: 2024-01-19 DOI: 10.1080/26896583.2023.2293493

Rose A Willett, Volodymyr P Tryndyak, Frederick A Beland, Igor P Pogribny

{"title":"Cellular and molecular alterations in a human hepatocellular in vitro model of nonalcoholic fatty liver disease development and stratification.","authors":"Rose A Willett, Volodymyr P Tryndyak, Frederick A Beland, Igor P Pogribny","doi":"10.1080/26896583.2023.2293493","DOIUrl":"10.1080/26896583.2023.2293493","url":null,"abstract":"The rapidly increasing incidence of nonalcoholic fatty liver disease (NAFLD) is a growing health crisis worldwide. If not detected early, NAFLD progression can lead to irreversible pathological states, including liver fibrosis and cirrhosis. Using in vitro models to understand the molecular pathogenesis has been extremely beneficial; however, most studies have utilized only short-term exposures, highlighting a limitation in current research to model extended fat-induced liver injury. We treated Hep3B cells continuously with a low dose of oleic and palmitic free fatty acids (FFAs) for 7 or 28 days. Transcriptomic analysis identified dysregulated molecular pathways and differential expression of 984 and 917 genes after FFA treatment for 7 and 28 days respectively. DNA methylation analysis of altered DNA methylated regions (DMRs) found 7 DMRs in common. Pathway analysis of differentially expressed genes (DEGs) revealed transcriptomic changes primarily involved in lipid metabolism, small molecule biochemistry, and molecular transport. Western blot analysis revealed changes in PDK4 and CPT1A protein levels, indicative of mitochondrial stress. In line with this, there was mitochondrial morphological change demonstrating breakdown of the mitochondrial network. This in vitro model of human NAFL mimics results observed in human patients and may be used as a pre-clinical model for drug intervention.","PeriodicalId":53200,"journal":{"name":"Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis","volume":" ","pages":"74-92"},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138799437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0