甜味剂、甘露醇和乳醇基因毒性作用的测定。

IF 1.2 4区 环境科学与生态学 Q4 ENVIRONMENTAL SCIENCES
Ebru Eker-Kartal, Ece Avuloglu-Yilmaz
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引用次数: 0

摘要

世界各地饮食习惯的变化导致食品中添加剂的使用增加。由于食品添加剂对健康的潜在影响一直存在争议,因此食品添加剂的安全性多年来一直是研究的主要焦点。本研究采用人外周血淋巴细胞染色体畸变(CAs)和微核(MN)检测方法,对甘露醇和乳醇这两种作为甜味剂食品添加剂的多元醇进行了体外遗传毒性评价。此外,还研究了这些甜味剂对核分裂指数(MI)和核分裂指数(NDI)的影响。甘露醇浓度分别为500、1000、2000、4000、8000 μg/mL,乳醇浓度分别为250、500、1000、2000、4000 μg/mL。结果表明,两种多元醇均不影响CA和MN频率,并且在所有处理浓度下均未引起NDI的显著变化。然而,甘露醇(除500和1000 μg/mL浓度外)和乳醇(除250 μg/mL浓度外)在几乎所有浓度和处理时间下均较对照组显著降低心肌梗死。综上所述,甘露醇和乳醇在体外培养的人淋巴细胞中没有显著的遗传毒性作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Determination of the genotoxic effects of sweeteners, mannitol and lactitol.

The changes in dietary habit around the world have led to an increased use of additives in the food. The safety of food additives has been a main focus of research for many years due to the ongoing debate on their potential effects on health. In this study, the in vitro genotoxic effects of mannitol and lactitol, polyols used as sweetener food additives, were evaluated using chromosomal aberrations (CAs) and micronucleus (MN) assays in human peripheral lymphocytes. Additionally, the effects of these sweeteners on the mitotic index (MI) and nuclear division index (NDI) were investigated. Concentrations of 500, 1000, 2000, 4000, and 8000 μg/mL for mannitol and 250, 500, 1000, 2000, and 4000 μg/mL for lactitol were used. The results indicated that both polyols did not affect CA and MN frequency, and did not cause a significant change in NDI at all treatment concentratoins. However, mannitol (except at concentrations of 500 and 1000 μg/mL) and lactitol (except at 250 μg/mL) significantly decreased the MI compared to the control at almost all concentrations and treatment times. In conclusion, it was observed that mannitol and lactitol did not have a significant genotoxic effect at the concentrations used in human lymphocytes in vitro.

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来源期刊
CiteScore
4.60
自引率
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发文量
10
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