人b淋巴母细胞样TK6细胞II期药物代谢酶的表达。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-01-01 Epub Date: 2022-03-11 DOI:10.1080/26896583.2022.2044242

Xilin Li, Yuxi Li, Kylie G Ning, Si Chen, Lei Guo, Jessica A Bonzo, Nan Mei

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引用次数: 1

摘要

体外遗传毒性检测在化学品风险评估中起着重要作用。人b淋巴母细胞样细胞系TK6被广泛用作监管安全性评估的标准细胞系。像许多其他哺乳动物细胞系一样，TK6细胞的代谢能力有限;因此，通常需要外源性代谢激活源用于遗传毒性试验。此前，我们开发了一组tk6衍生的细胞系，这些细胞系分别表达14种细胞色素p450 (CYPs)中的一种。在本研究中，我们调查了一组主要的II期药物代谢酶，以表征它们在TK6细胞中的基线表达。这些结果可以作为这种常用细胞系的参考酶谱。

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The expression of Phase II drug-metabolizing enzymes in human B-lymphoblastoid TK6 cells.

In vitro genotoxicity testing plays an important role in chemical risk assessment. The human B-lymphoblastoid cell line TK6 is widely used as a standard cell line for regulatory safety evaluations. Like many other mammalian cell lines, TK6 cells have limited metabolic capacity; therefore, usually require a source of exogenous metabolic activation for use in genotoxicity testing. Previously, we developed a set of TK6-derived cell lines that individually express one of fourteen cytochrome P450s (CYPs). In the present study, we surveyed a panel of major Phase II drug-metabolizing enzymes to characterize their baseline expression in TK6 cells. These results may serve as a reference enzymatic profile of this commonly used cell line.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.