Chemical & Biomedical Imaging最新文献_第8页

Probing Electrochemical Strain Generation in Vanadium Oxide Cathodes during Cycling of Aqueous Zinc-Ion Batteries via Imaging Technique 利用成像技术探测锌离子电池循环过程中氧化钒阴极产生的电化学应变

Chemical & Biomedical Imaging Pub Date : 2025-03-16 DOI: 10.1021/cbmi.5c0000310.1021/cbmi.5c00003

Bret A. Marckx, Hunter Maclennan and Ömer Özgür Capraz*,

{"title":"Probing Electrochemical Strain Generation in Vanadium Oxide Cathodes during Cycling of Aqueous Zinc-Ion Batteries via Imaging Technique","authors":"Bret A. Marckx, Hunter Maclennan and Ömer Özgür Capraz*, ","doi":"10.1021/cbmi.5c0000310.1021/cbmi.5c00003","DOIUrl":"https://doi.org/10.1021/cbmi.5c00003https://doi.org/10.1021/cbmi.5c00003","url":null,"abstract":"Aqueous batteries have received a great deal of attention for grid-scale energy storage applications but suffer from low-capacity retention and utilization. A lack of understanding of chemomechanical instabilities and charge storage mechanisms in cathodes limits the development of advanced aqueous batteries. To shed light on these instabilities, operando techniques are necessary to probe the complex interplay between electrochemistry and mechanics during cycling. Here, we report an operando technique to probe electrochemical strains in cathodes in aqueous electrolytes during battery cycling via optical imaging and digital image correlation. Operando mechanical measurements indicate that the cathode undergoes positive strain generation during discharge and negative generation during charge. Strain derivatives reveal a close correlation between electrochemical and mechanical behaviors, highlighting the connection between electrochemistry and mechanics. This operando imaging technique is broadly applicable and paves the way for a deeper understanding of deformation mechanisms in aqueous, multivalent ion battery materials.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 6","pages":"352–358 352–358"},"PeriodicalIF":0.0,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.5c00003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144338103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probing Electrochemical Strain Generation in Vanadium Oxide Cathodes during Cycling of Aqueous Zinc-Ion Batteries via Imaging Technique. 利用成像技术探测锌离子电池循环过程中氧化钒阴极产生的电化学应变。

Chemical & Biomedical Imaging Pub Date : 2025-03-16 eCollection Date: 2025-06-23 DOI: 10.1021/cbmi.5c00003

Bret A Marckx, Hunter Maclennan, Ömer Özgür Capraz

{"title":"Probing Electrochemical Strain Generation in Vanadium Oxide Cathodes during Cycling of Aqueous Zinc-Ion Batteries via Imaging Technique.","authors":"Bret A Marckx, Hunter Maclennan, Ömer Özgür Capraz","doi":"10.1021/cbmi.5c00003","DOIUrl":"10.1021/cbmi.5c00003","url":null,"abstract":"Aqueous batteries have received a great deal of attention for grid-scale energy storage applications but suffer from low-capacity retention and utilization. A lack of understanding of chemomechanical instabilities and charge storage mechanisms in cathodes limits the development of advanced aqueous batteries. To shed light on these instabilities, operando techniques are necessary to probe the complex interplay between electrochemistry and mechanics during cycling. Here, we report an operando technique to probe electrochemical strains in cathodes in aqueous electrolytes during battery cycling via optical imaging and digital image correlation. Operando mechanical measurements indicate that the cathode undergoes positive strain generation during discharge and negative generation during charge. Strain derivatives reveal a close correlation between electrochemical and mechanical behaviors, highlighting the connection between electrochemistry and mechanics. This operando imaging technique is broadly applicable and paves the way for a deeper understanding of deformation mechanisms in aqueous, multivalent ion battery materials.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 6","pages":"352-358"},"PeriodicalIF":0.0,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12188393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144509407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Droplet Wettability on Scanning Electrochemical Cell Microscopy Performance in Stainless Steels 液滴润湿性对不锈钢扫描电化学显微镜性能的影响

Chemical & Biomedical Imaging Pub Date : 2025-03-13 DOI: 10.1021/cbmi.4c0010110.1021/cbmi.4c00101

Sarah R. Yassine, León Zendejas Medina, Egor Katkov, Robert Lacasse and Janine Mauzeroll*,

引用次数: 0

Impact of Droplet Wettability on Scanning Electrochemical Cell Microscopy Performance in Stainless Steels. 液滴润湿性对不锈钢扫描电化学显微镜性能的影响。

Chemical & Biomedical Imaging Pub Date : 2025-03-13 eCollection Date: 2025-04-28 DOI: 10.1021/cbmi.4c00101

Sarah R Yassine, León Zendejas Medina, Egor Katkov, Robert Lacasse, Janine Mauzeroll

引用次数: 0

Real-Time Visualization of Endogenous H2O2 Production in Mammalian Spheroids by Electrochemiluminescence 利用电化学发光技术实时可视化哺乳动物球体内源性H2O2生成

Chemical & Biomedical Imaging Pub Date : 2025-03-10 DOI: 10.1021/cbmi.4c0010510.1021/cbmi.4c00105

Vanshika Gupta, Francesco Falciani, Brady R. Layman, Megan L. Hill, Stefania Rapino* and Jeffrey E. Dick*,

{"title":"Real-Time Visualization of Endogenous H2O2 Production in Mammalian Spheroids by Electrochemiluminescence","authors":"Vanshika Gupta, Francesco Falciani, Brady R. Layman, Megan L. Hill, Stefania Rapino* and Jeffrey E. Dick*, ","doi":"10.1021/cbmi.4c0010510.1021/cbmi.4c00105","DOIUrl":"https://doi.org/10.1021/cbmi.4c00105https://doi.org/10.1021/cbmi.4c00105","url":null,"abstract":"Two-dimensional cell culture may be insufficient when it comes to understanding human disease. The redox behavior of complex, three-dimensional tissue is critical to understanding disease genesis and propagation. Unfortunately, few measurement tools are available for such three-dimensional models to yield quantitative insight into how reactive oxygen species (ROS) form over time. Here, we demonstrate an imaging platform for the real-time visualization of H2O2 formation for mammalian spheroids made of noncancerous human embryonic kidney cells (HEK-293) and metastatic breast cancer cells (MCF-7 and MDA-MB-231). We take advantage of the luminol and H2O2 electrochemiluminescence reaction on a transparent tin-doped indium oxide electrode. The luminescence of this reaction as a function of [H2O2] is linear (R2 = 0.98) with a dynamic range between 0.5 μM to 0.1 mM, and limit of detection of 2.26 ± 0.58 μM. Our method allows for the observation of ROS activity in growing spheroids days in advance of current techniques without the need to sacrifice the sample postanalysis. Finally, we use our procedure to demonstrate how key ROS pathways in cancerous spheroids can be up-regulated and downregulated through the addition of common metabolic drugs, rotenone and carbonyl cyanide-p-trifluoromethoxyphenylhydrazone. Our results suggest that the Warburg Effect can be studied for single mammalian cancerous spheroids, and the use of metabolic drugs allows one to implicate specific metabolic pathways in ROS formation. We expect this diagnostic tool to have wide applications in understanding the real-time propagation of human disease in a system more closely related to human tissue.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 5","pages":"310–321 310–321"},"PeriodicalIF":0.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00105","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144133895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-Time Visualization of Endogenous H₂O₂ Production in Mammalian Spheroids by Electrochemiluminescence. 利用电化学发光技术实时可视化哺乳动物球体内源性H2O2生成。

Chemical & Biomedical Imaging Pub Date : 2025-03-10 eCollection Date: 2025-05-26 DOI: 10.1021/cbmi.4c00105

Vanshika Gupta, Francesco Falciani, Brady R Layman, Megan L Hill, Stefania Rapino, Jeffrey E Dick

{"title":"Real-Time Visualization of Endogenous H2O2 Production in Mammalian Spheroids by Electrochemiluminescence.","authors":"Vanshika Gupta, Francesco Falciani, Brady R Layman, Megan L Hill, Stefania Rapino, Jeffrey E Dick","doi":"10.1021/cbmi.4c00105","DOIUrl":"10.1021/cbmi.4c00105","url":null,"abstract":"Two-dimensional cell culture may be insufficient when it comes to understanding human disease. The redox behavior of complex, three-dimensional tissue is critical to understanding disease genesis and propagation. Unfortunately, few measurement tools are available for such three-dimensional models to yield quantitative insight into how reactive oxygen species (ROS) form over time. Here, we demonstrate an imaging platform for the real-time visualization of H2O2 formation for mammalian spheroids made of noncancerous human embryonic kidney cells (HEK-293) and metastatic breast cancer cells (MCF-7 and MDA-MB-231). We take advantage of the luminol and H2O2 electrochemiluminescence reaction on a transparent tin-doped indium oxide electrode. The luminescence of this reaction as a function of [H2O2] is linear (R 2 = 0.98) with a dynamic range between 0.5 μM to 0.1 mM, and limit of detection of 2.26 ± 0.58 μM. Our method allows for the observation of ROS activity in growing spheroids days in advance of current techniques without the need to sacrifice the sample postanalysis. Finally, we use our procedure to demonstrate how key ROS pathways in cancerous spheroids can be up-regulated and downregulated through the addition of common metabolic drugs, rotenone and carbonyl cyanide-p-trifluoromethoxyphenylhydrazone. Our results suggest that the Warburg Effect can be studied for single mammalian cancerous spheroids, and the use of metabolic drugs allows one to implicate specific metabolic pathways in ROS formation. We expect this diagnostic tool to have wide applications in understanding the real-time propagation of human disease in a system more closely related to human tissue.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 5","pages":"310-321"},"PeriodicalIF":0.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12117415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Molecule Fluorescence Imaging of Energy-Related Catalytic Reactions 能量相关催化反应的单分子荧光成像

Chemical & Biomedical Imaging Pub Date : 2025-03-07 DOI: 10.1021/cbmi.4c0011210.1021/cbmi.4c00112

Qingdian Yan, Xianghong Li, Jianbin Luo and Ming Zhao*,

{"title":"Single-Molecule Fluorescence Imaging of Energy-Related Catalytic Reactions","authors":"Qingdian Yan, Xianghong Li, Jianbin Luo and Ming Zhao*, ","doi":"10.1021/cbmi.4c0011210.1021/cbmi.4c00112","DOIUrl":"https://doi.org/10.1021/cbmi.4c00112https://doi.org/10.1021/cbmi.4c00112","url":null,"abstract":"The pressing challenges of the energy crisis and environmental problems necessitate the pursuit of efficient and sustainable energy conversion technologies, wherein catalytic processes play a vital role in addressing these issues. Single-molecule fluorescence microscopy (SMFM) offers a transformative approach to understanding various catalytic reactions by enabling real-time visualization of molecular adsorption, diffusion, and transformation on catalytic surfaces. The unprecedented insights into the spatial distribution of active sites, catalytic heterogeneity, and the dynamics of key intermediates result in single- or subparticle level structure–property relations, thereby offering insightful perspectives for catalyst design and mechanistic understanding of energy-related catalytic processes. In this review, we provide an overview of the recent progress in using SMFM for investigating energy-related catalytic reactions. The advancement in SMFM imaging techniques for investigating nonfluorescent chemical processes is also highlighted. Finally, we conclude the review by commenting on the current challenges and prospects in advancing SMFM in energy research. We hope that the capable SMFM imaging techniques and insights will promote the development and realistic application of various energy-related catalytic reactions, together with inspiring researchers to explore the power of SMFM in other applications.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 5","pages":"280–300 280–300"},"PeriodicalIF":0.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144133940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Molecule Fluorescence Imaging of Energy-Related Catalytic Reactions. 能量相关催化反应的单分子荧光成像。

Chemical & Biomedical Imaging Pub Date : 2025-03-07 eCollection Date: 2025-05-26 DOI: 10.1021/cbmi.4c00112

Qingdian Yan, Xianghong Li, Jianbin Luo, Ming Zhao

{"title":"Single-Molecule Fluorescence Imaging of Energy-Related Catalytic Reactions.","authors":"Qingdian Yan, Xianghong Li, Jianbin Luo, Ming Zhao","doi":"10.1021/cbmi.4c00112","DOIUrl":"10.1021/cbmi.4c00112","url":null,"abstract":"The pressing challenges of the energy crisis and environmental problems necessitate the pursuit of efficient and sustainable energy conversion technologies, wherein catalytic processes play a vital role in addressing these issues. Single-molecule fluorescence microscopy (SMFM) offers a transformative approach to understanding various catalytic reactions by enabling real-time visualization of molecular adsorption, diffusion, and transformation on catalytic surfaces. The unprecedented insights into the spatial distribution of active sites, catalytic heterogeneity, and the dynamics of key intermediates result in single- or subparticle level structure-property relations, thereby offering insightful perspectives for catalyst design and mechanistic understanding of energy-related catalytic processes. In this review, we provide an overview of the recent progress in using SMFM for investigating energy-related catalytic reactions. The advancement in SMFM imaging techniques for investigating nonfluorescent chemical processes is also highlighted. Finally, we conclude the review by commenting on the current challenges and prospects in advancing SMFM in energy research. We hope that the capable SMFM imaging techniques and insights will promote the development and realistic application of various energy-related catalytic reactions, together with inspiring researchers to explore the power of SMFM in other applications.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 5","pages":"280-300"},"PeriodicalIF":0.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12117407/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nonlight-Driven Aggregation-Induced Emission Luminogens for Bioimaging and Theranostics. 用于生物成像和治疗的非光驱动聚集诱导发射发光源。

Chemical & Biomedical Imaging Pub Date : 2025-03-04 eCollection Date: 2025-06-23 DOI: 10.1021/cbmi.4c00108

Yong Tian, Weigeng Huang, Zhijia Sheng, Dingyuan Yan, Dong Wang, Ben Zhong Tang

{"title":"Nonlight-Driven Aggregation-Induced Emission Luminogens for Bioimaging and Theranostics.","authors":"Yong Tian, Weigeng Huang, Zhijia Sheng, Dingyuan Yan, Dong Wang, Ben Zhong Tang","doi":"10.1021/cbmi.4c00108","DOIUrl":"10.1021/cbmi.4c00108","url":null,"abstract":"Aggregation-induced emission luminogens (AIEgens) have been prosperously developed and applied in the fields of optical imaging and theranostics since its establishment. Nowadays, AIEgens can fulfill nearly all requirements in optical imaging and theranostics with emission spectra ranging from visible to near-infrared wavelengths. Although a variety of AIEgens with varying wavelengths and functionalities have been continuously designed, their performance is heavily dependent on the use of conventional light sources, such as xenon lamps and lasers, which severely hinder further applications due to limited penetration depth and background autofluorescence in biological tissues. To mitigate these limitations and maximize the potential of AIEgens, unconventional excitation sources such as chemical energy, ultrasound, and X-ray offer effective alternatives that circumvent the drawbacks associated with traditional light-based constant excitation. In this Review, we introduce the fundamental principles governing the combination of unconventional excitation sources with AIEgens, highlight recent advancements in using AIEgens excited by these unconventional sources for bioimaging and theranostics, and discuss current challenges and future perspectives aimed at advancing the biomedical applications of AIEgens.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 6","pages":"341-351"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12188483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144509405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Albumin Encapsulation of Cyanine Dye for High-Performance NIR-II Imaging-Guided Photodynamic Therapy 高效NIR-II成像引导光动力治疗中花青素染料白蛋白包封。

IF 5.7

Chemical & Biomedical Imaging Pub Date : 2025-03-04 DOI: 10.1021/cbmi.5c00005

Lang Bai, Yiyang Jia, Zihan Wang, Zeen Wang, Yunlong Jia, Yuewei Zhang and Shoujun Zhu*,

{"title":"Albumin Encapsulation of Cyanine Dye for High-Performance NIR-II Imaging-Guided Photodynamic Therapy","authors":"Lang Bai, Yiyang Jia, Zihan Wang, Zeen Wang, Yunlong Jia, Yuewei Zhang and Shoujun Zhu*, ","doi":"10.1021/cbmi.5c00005","DOIUrl":"10.1021/cbmi.5c00005","url":null,"abstract":"Albumin encapsulation is a powerful strategy for drug delivery, yet its potential has not been fully explored for photodynamic therapy (PDT) agents. Cl-containing near-infrared (NIR) cyanine dyes are intrinsically PDT agents and tend to covalently bind with albumin; however, their PDT efficiency in tumors is largely compromised due to limited accumulation of the complex (size less than 10 nm) to the tumor site. To maximize their PDT effect while retaining sufficient NIR brightness for imaging-guided PDT, we developed a DTT-promoted encapsulation strategy to enhance singlet oxygen release for Cl-containing dyes. By disrupting disulfide bonds in albumin, the protein shell is loosened, increasing size while maintaining singlet oxygen release, partial brightness, and photostability. In vivo experiments reveal the rapid tumor accumulation of IR-6B3@DTT-HSA, enabling flexible treatment timing. This strategy enhances targeted delivery and PDT efficacy, paving the way for broader applications in cancer therapy.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 7","pages":"424–432"},"PeriodicalIF":5.7,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12308594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144762325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0