Chemical & Biomedical Imaging最新文献_第8页

Peptide-Based Turn-On Fluorescent Probes for Highly Specific Detection of Survivin Protein in the Cancer Cells 用于高度特异性检测癌细胞中 Survivin 蛋白的肽基开启荧光探针

Chemical & Biomedical Imaging Pub Date : 2024-05-01 DOI: 10.1021/cbmi.4c00017

Takeshi Fuchigami*, Tomoe Nakayama, Yusuke Miyanari, Iori Nozaki, Natsumi Ishikawa, Ayako Tagawa, Sakura Yoshida, Masayuki Munekane, Morio Nakayama and Kazuma Ogawa,

{"title":"Peptide-Based Turn-On Fluorescent Probes for Highly Specific Detection of Survivin Protein in the Cancer Cells","authors":"Takeshi Fuchigami*, Tomoe Nakayama, Yusuke Miyanari, Iori Nozaki, Natsumi Ishikawa, Ayako Tagawa, Sakura Yoshida, Masayuki Munekane, Morio Nakayama and Kazuma Ogawa, ","doi":"10.1021/cbmi.4c00017","DOIUrl":"10.1021/cbmi.4c00017","url":null,"abstract":"Survivin is highly expressed in most human cancers, making it a promising target for cancer diagnosis and treatment. In this study, we developed peptide probes consisting of Bor65–75, a high-affinity survivin-binding peptide, and a survivin protein segment using peptide linkers as survivin-sensitive fluorescent probes (SSFPs). All conjugates were attached to 5(6)-carboxyfluorescein (FAM) at the C-terminal as a fluorophore and to 4((4(dimethylamino)phenyl)azo)benzoic acid (DABCYL) at the N-terminal as a quencher. Fluorescence (or Förster) resonance energy transfer (FRET) quenching via intramolecular binding of Bor65–75 with survivin protein segment could be diminished by the approach of survivin to SSFPs, which dissociate Bor65–75 from SSPF and increased the distance between FAM and DABCYL. A binding assay using recombinant human survivin protein (rSurvivin) demonstrated moderate to high affinity of SSFPs for survivin (dissociation constants (Kd) = 121–1740 nM). Although the SSFPs (0.5 μM) had almost no fluorescence under baseline conditions, a dose-dependent increase in fluorescence intensity was observed in the presence of rSurvivin (0.1–2.0 μM). In particular, the proline-rich SSFP (SSFP5) showed the highest (2.7-fold) fluorescence induction at 2.0 μM survivin compared to the signals in the absence of survivin. Confocal fluorescence imaging demonstrated that SSFP5 exhibited clear fluorescence signals in survivin-positive MDA-MB-231 cells, whereas no marked fluorescence signals were observed in survivin-negative MCF-10A cells. Collectively, these results suggest that SSFPs can be used as survivin-specific FRET imaging probes.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 5","pages":"374–383"},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141035076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Progress in Molecular Probes for Imaging of Acute Kidney Injury 急性肾损伤成像分子探针的最新进展

Chemical & Biomedical Imaging Pub Date : 2024-05-01 DOI: 10.1021/cbmi.4c0002410.1021/cbmi.4c00024

Muqadas Sitara, Wangning Zhang, Han Gao, Jiwei Li* and Jiangwei Tian*,

{"title":"Recent Progress in Molecular Probes for Imaging of Acute Kidney Injury","authors":"Muqadas Sitara, Wangning Zhang, Han Gao, Jiwei Li* and Jiangwei Tian*, ","doi":"10.1021/cbmi.4c0002410.1021/cbmi.4c00024","DOIUrl":"https://doi.org/10.1021/cbmi.4c00024https://doi.org/10.1021/cbmi.4c00024","url":null,"abstract":"Acute kidney injury (AKI), a prevalent and complex clinical condition associated with elevated risks of morbidity and mortality, necessitates the meticulous detection and monitoring of kidney damage globally. Biomedicine has shown keen interest in molecular probes and detectors for AKI due to their sensitivity, rapidity, and cost-effectiveness. Bioimaging technologies play a significant role in identifying and quantifying AKI indicators, enhancing diagnostic approaches, and potentially refining clinical therapies for immediate injury control. Molecular probes serve as valuable tools for drug screening, uncovering renoprotective components, signaling pathways, and the nephrotoxic effects of drugs. This review comprehensively summarizes the latest advancements in molecular probes, emphasizing their exceptional efficacy in various characteristics, including renal cleanability, multichannel detection capability, near-infrared-II responsiveness, and reactivity toward reactive oxygen species. These probes offer enhanced benefits for assessing kidney damage and evaluating the therapeutic effects of medications while simultaneously reducing toxic effects. Additionally, the review delves into future potentials and challenges in this field, aiming to inspire the development and enhancement of molecular bioimaging for the early diagnosis and treatment of kidney diseases.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 8","pages":"526–541 526–541"},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142075704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling the Thermoelastic Sample Response for Subdiffraction Infrared Spectroscopic Imaging 为亚衍射红外光谱成像建立热弹性样品响应模型

Chemical & Biomedical Imaging Pub Date : 2024-04-29 DOI: 10.1021/cbmi.4c00018

Seth Kenkel, and , Rohit Bhargava*,

{"title":"Modeling the Thermoelastic Sample Response for Subdiffraction Infrared Spectroscopic Imaging","authors":"Seth Kenkel,  and , Rohit Bhargava*, ","doi":"10.1021/cbmi.4c00018","DOIUrl":"10.1021/cbmi.4c00018","url":null,"abstract":"There is significant and increasing interest in using the photothermal effect to record infrared (IR) absorption spectra localized to volumes that are considerably smaller than the wavelength of excitation, i.e., subdiffraction imaging. As opposed to conventional IR microscopy, in which absorption and scattering of the illuminating light is measured, subdiffraction imaging can be achieved through detection of the sample’s thermal response to IR absorption-induced heating. While this relationship has been examined by a variety of coarse-grained models, a generalized analysis of the dependence of temperature and surface deformation arising from an absorber below the surface has not been reported. Here, we present an analytical model to understand a sample’s thermoelastic response in photothermal measurements. The model shows important dependence of the ability to record subdiffraction data on modulation frequency of exciting light, limitations imposed by optical sensing, and the potential to discern location of objects ultimately limited by noise and sharpness of the detecting mechanism. This foundational analysis should allow for better modeling, understanding, and harnessing of the relationship between absorption and sample response that underlies IR photothermal measurements.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 6","pages":"413–421"},"PeriodicalIF":0.0,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11200252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141472566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-Time Tracking of Vesicles in Living Cells Reveals That Tau-Hyperphosphorylation Suppresses Unidirectional Transport by Motor Proteins 实时跟踪活细胞中的囊泡发现 Tau 过度磷酸化抑制了马达蛋白的单向运输

Chemical & Biomedical Imaging Pub Date : 2024-04-23 DOI: 10.1021/cbmi.4c00016

Eunsang Lee, Donghee Kim, Yo Han Song, Kyujin Shin, Sanggeun Song, Minho Lee, Yeongchang Goh, Mi Hee Lim, Ji-Hyun Kim, Jaeyoung Sung* and Kang Taek Lee*,

{"title":"Real-Time Tracking of Vesicles in Living Cells Reveals That Tau-Hyperphosphorylation Suppresses Unidirectional Transport by Motor Proteins","authors":"Eunsang Lee, Donghee Kim, Yo Han Song, Kyujin Shin, Sanggeun Song, Minho Lee, Yeongchang Goh, Mi Hee Lim, Ji-Hyun Kim, Jaeyoung Sung* and Kang Taek Lee*, ","doi":"10.1021/cbmi.4c00016","DOIUrl":"https://doi.org/10.1021/cbmi.4c00016","url":null,"abstract":"Synaptic vesicle transport by motor proteins along microtubules is a crucially active process underlying neuronal communication. It is known that microtubules are destabilized by tau-hyperphosphorylation, which causes tau proteins to detach from microtubules and form neurofibril tangles. However, how tau-phosphorylation affects the transport dynamics of motor proteins on the microtubule remains unknown. Here, we discover that the long-distance unidirectional motion of vesicle-motor protein multiplexes (VMPMs) in living cells is suppressed under tau-hyperphosphorylation, with the consequent loss of fast vesicle-transport along the microtubule. The VMPMs in hyperphosphorylated cells exhibit seemingly bidirectional random motion, with dynamic properties far different from those of VMPM motion in normal cells. We establish a parsimonious physicochemical model of VMPM’s active motion that provides a unified, quantitative explanation and predictions for our experimental results. Our analysis reveals that, under hyperphosphorylation conditions, motor protein multiplexes have both static and dynamic motility fluctuations. The loss of fast vesicle-transport along the microtubule can be a mechanism of neurodegenerative disorders associated with tau-hyperphosphorylation.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 5","pages":"362–373"},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141156243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multivariate Analysis of Individual Bacterial Outer Membrane Vesicles Using Fluorescence Microscopy 利用荧光显微镜对单个细菌外膜囊泡进行多元分析

Chemical & Biomedical Imaging Pub Date : 2024-04-19 DOI: 10.1021/cbmi.4c00014

Aarshi N. Singh, Justin B Nice, Meishan Wu, Angela C. Brown and Nathan J. Wittenberg*,

{"title":"Multivariate Analysis of Individual Bacterial Outer Membrane Vesicles Using Fluorescence Microscopy","authors":"Aarshi N. Singh, Justin B Nice, Meishan Wu, Angela C. Brown and Nathan J. Wittenberg*, ","doi":"10.1021/cbmi.4c00014","DOIUrl":"10.1021/cbmi.4c00014","url":null,"abstract":"Gram-negative bacteria produce outer membrane vesicles (OMVs) that play a critical role in cell–cell communication and virulence. OMVs have emerged as promising therapeutic agents for various biological applications such as vaccines and targeted drug delivery. However, the full potential of OMVs is currently constrained by inherent heterogeneities, such as size and cargo differences, and traditional ensemble assays are limited in their ability to reveal OMV heterogeneity. To overcome this issue, we devised an innovative approach enabling the identification of various characteristics of individual OMVs. This method, employing fluorescence microscopy, facilitates the detection of variations in size and surface markers. To demonstrate our method, we utilize the oral bacterium Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) which produces OMVs with a bimodal size distribution. As part of its virulence, A. actinomycetemcomitans secretes leukotoxin (LtxA) in two forms: soluble and surface associated with the OMVs. We observed a correlation between the size and toxin presence where larger OMVs were much more likely to possess LtxA compared to the smaller OMVs. In addition, we noted that, among the smallest OMVs (<100 nm diameter), the fractions that are toxin positive range from 0 to 30%, while the largest OMVs (>200 nm diameter) are between 70 and 100% toxin positive.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 5","pages":"352–361"},"PeriodicalIF":0.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140684259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements and Practical Considerations for Biophysical Research: Navigating the Challenges and Future of Super-resolution Microscopy 生物物理研究的进展和实际考虑因素：驾驭超分辨率显微镜的挑战与未来

Chemical & Biomedical Imaging Pub Date : 2024-04-19 DOI: 10.1021/cbmi.4c00019

Huanhuan Chen, Guangjie Yan, Meng-Hsuan Wen, Kameron N. Brooks, Yuteng Zhang, Pei-San Huang and Tai-Yen Chen*,

{"title":"Advancements and Practical Considerations for Biophysical Research: Navigating the Challenges and Future of Super-resolution Microscopy","authors":"Huanhuan Chen, Guangjie Yan, Meng-Hsuan Wen, Kameron N. Brooks, Yuteng Zhang, Pei-San Huang and Tai-Yen Chen*, ","doi":"10.1021/cbmi.4c00019","DOIUrl":"10.1021/cbmi.4c00019","url":null,"abstract":"The introduction of super-resolution microscopy (SRM) has significantly advanced our understanding of cellular and molecular dynamics, offering a detailed view previously beyond our reach. Implementing SRM in biophysical research, however, presents numerous challenges. This review addresses the crucial aspects of utilizing SRM effectively, from selecting appropriate fluorophores and preparing samples to analyzing complex data sets. We explore recent technological advancements and methodological improvements that enhance the capabilities of SRM. Emphasizing the integration of SRM with other analytical methods, we aim to overcome inherent limitations and expand the scope of biological insights achievable. By providing a comprehensive guide for choosing the most suitable SRM methods based on specific research objectives, we aim to empower researchers to explore complex biological processes with enhanced precision and clarity, thereby advancing the frontiers of biophysical research.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 5","pages":"331–344"},"PeriodicalIF":0.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140683836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Imaging-Assisted Antisense Oligonucleotide Delivery for Tumor-Targeted Gene Therapy 成像辅助反义寡核苷酸递送用于肿瘤靶向基因疗法

Chemical & Biomedical Imaging Pub Date : 2024-04-17 DOI: 10.1021/cbmi.4c00012

Hanwen Liao, Siyi Wang, Xiaoning Wang, David Zixiang Dai, Yan Zhang*, Chenghong Zhu* and Jinbo Li*,

引用次数: 0

Methylene Blue: An FDA-Approved NIR-II Fluorogenic Probe with Extremely Low pH Responsibility for Hyperchlorhydria Imaging 亚甲基蓝：用于高氯酸血症成像的 FDA 批准的具有极低 pH 值的 NIR-II 荧光探针

Chemical & Biomedical Imaging Pub Date : 2024-04-12 DOI: 10.1021/cbmi.4c0001110.1021/cbmi.4c00011

Guanjun Deng, Siwei Zhang, Xinghua Peng, Gongcheng Ma, Luxuan Liu, Yuyu Tan, Ping Gong*, Ben Zhong Tang*, Lintao Cai* and Pengfei Zhang*,

引用次数: 0

An Activatable Semiconducting Nanoprobe for Early and Accurate Diagnosis of Liver Injury. 用于早期准确诊断肝损伤的可激活半导体纳米探针

Chemical & Biomedical Imaging Pub Date : 2024-04-01 eCollection Date: 2024-08-26 DOI: 10.1021/cbmi.4c00022

Fei Li, Shaobin Wu, Keyang Li, Jun Zhu, Shasha He, Huayu Tian

引用次数: 0

An Activatable Semiconducting Nanoprobe for Early and Accurate Diagnosis of Liver Injury 用于早期准确诊断肝损伤的可激活半导体纳米探针

Chemical & Biomedical Imaging Pub Date : 2024-04-01 DOI: 10.1021/cbmi.4c0002210.1021/cbmi.4c00022

Fei Li, Shaobin Wu, Keyang Li, Jun Zhu, Shasha He* and Huayu Tian*,

{"title":"An Activatable Semiconducting Nanoprobe for Early and Accurate Diagnosis of Liver Injury","authors":"Fei Li, Shaobin Wu, Keyang Li, Jun Zhu, Shasha He* and Huayu Tian*, ","doi":"10.1021/cbmi.4c0002210.1021/cbmi.4c00022","DOIUrl":"https://doi.org/10.1021/cbmi.4c00022https://doi.org/10.1021/cbmi.4c00022","url":null,"abstract":"Liver injury, caused by factors like viral hepatitis and drug overdose, poses a significant health risk, with current diagnostic methods lacking specificity, increasing the need for more precise molecular imaging techniques. Herein, we present an activatable semiconducting liver injury reporter (SLIR) for early and accurate diagnosis of liver injury. The SLIR, which is composed of semiconducting polymers with an electron-withdrawing quenching segment, remains nonfluorescent until it encounters biothiols such as cysteine in the liver. SLIR accumulates efficiently in the liver and respond rapidly to biothiols, allowing accurate and early detection of liver damage. The recovery of SLIR fluorescence negatively reflects the dynamics of oxidative stress in the liver and provides information on the severity of tissue damage. Thus, the specificity of SLIR, the fast response, and the efficient targeting of the liver make it a promising tool for the precise diagnosis of liver damage at an early stage.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 8","pages":"569–576 569–576"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142075408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0