Chemical & Biomedical Imaging最新文献_第8页

Lipid Metabolic Heterogeneity during Early Embryogenesis Revealed by Hyper-3D Stimulated Raman Imaging 超3d刺激拉曼成像揭示早期胚胎发生过程中的脂质代谢异质性

Chemical & Biomedical Imaging Pub Date : 2024-10-04 DOI: 10.1021/cbmi.4c0005510.1021/cbmi.4c00055

Jie Huang, Ling Zhang, Ninghui Shao, Yongqing Zhang, Yuyan Xu, Yihui Zhou, Delong Zhang, Jin Zhang* and Hyeon Jeong Lee*,

{"title":"Lipid Metabolic Heterogeneity during Early Embryogenesis Revealed by Hyper-3D Stimulated Raman Imaging","authors":"Jie Huang, Ling Zhang, Ninghui Shao, Yongqing Zhang, Yuyan Xu, Yihui Zhou, Delong Zhang, Jin Zhang* and Hyeon Jeong Lee*, ","doi":"10.1021/cbmi.4c0005510.1021/cbmi.4c00055","DOIUrl":"https://doi.org/10.1021/cbmi.4c00055https://doi.org/10.1021/cbmi.4c00055","url":null,"abstract":"Studying embryogenesis is fundamental to understanding developmental biology and reproductive medicine. Its process requires precise spatiotemporal regulations in which lipid metabolism plays a crucial role. However, the spatial dynamics of lipid species at the subcellular level remains obscure due to technical limitations. To address this challenge, we developed a hyperspectral 3D imaging and analysis method based on stimulated Raman scattering microscopy (hyper-3D SRS) to quantitatively assess lipid profiles in individual embryos through submicrometer resolution (x–y), 3D optical sectioning (z), and chemical bond-selective (Ω) imaging. Using hyper-3D SRS, individual lipid droplets (LDs) in single cells were identified and quantified. Our findings revealed that the LD profiles within a single embryo are not uniform, even as early as the 2-cell stage. Notably, we also discovered a dynamic relationship between the LD size and unsaturation degree as embryos develop, indicating diverse lipid metabolism during early development. Furthermore, abnormal LDs were observed in oocytes of a progeria mouse model, suggesting that LDs could serve as a potential biomarker for assessing oocyte/embryo quality. Overall, our results highlight the potential of hyper-3D SRS as a noninvasive method for studying lipid content, composition, and subcellular distribution in embryos. This technique provides valuable insights into lipid metabolism during embryonic development and has the potential for clinical applications in evaluating oocyte/embryo quality.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 1","pages":"15–24 15–24"},"PeriodicalIF":0.0,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00055","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143091820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring Macrophage Polarization with Gene Expression Reporters and Bioluminescence Phasor Analysis. 利用基因表达报告和生物发光相量分析监测巨噬细胞极化。

Chemical & Biomedical Imaging Pub Date : 2024-10-03 eCollection Date: 2024-11-25 DOI: 10.1021/cbmi.4c00049

Giulia Tedeschi, Mariana X Navarro, Lorenzo Scipioni, Tanvi K Sondhi, Jennifer A Prescher, Michelle A Digman

{"title":"Monitoring Macrophage Polarization with Gene Expression Reporters and Bioluminescence Phasor Analysis.","authors":"Giulia Tedeschi, Mariana X Navarro, Lorenzo Scipioni, Tanvi K Sondhi, Jennifer A Prescher, Michelle A Digman","doi":"10.1021/cbmi.4c00049","DOIUrl":"10.1021/cbmi.4c00049","url":null,"abstract":"Macrophages exhibit a spectrum of behaviors upon activation and are generally classified as one of two types: inflammatory (M1) or anti-inflammatory (M2). Tracking these phenotypes in living cells can provide insight into immune function but remains a challenging pursuit. Existing methods are mostly limited to static readouts or are difficult to employ for multiplexed imaging in complex 3D environments while maintaining cellular resolution. We aimed to fill this void using bioluminescent technologies. Here we report genetically engineered luciferase reporters for the long-term monitoring of macrophage polarization via spectral phasor analysis. M1- and M2-specific promoters were used to drive the expression of bioluminescent enzymes in macrophage cell lines. The readouts were multiplexed and discernible in both 2D and 3D formats with single-cell resolution in living samples. Collectively, this work expands the toolbox of methods for monitoring macrophage polarization and provides a blueprint for monitoring other multifaceted networks in heterogeneous environments.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 11","pages":"765-774"},"PeriodicalIF":0.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11600157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142752150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring Macrophage Polarization with Gene Expression Reporters and Bioluminescence Phasor Analysis 利用基因表达报告和生物发光相位分析监测巨噬细胞极化

Chemical & Biomedical Imaging Pub Date : 2024-10-03 DOI: 10.1021/cbmi.4c0004910.1021/cbmi.4c00049

Giulia Tedeschi, Mariana X. Navarro, Lorenzo Scipioni, Tanvi K. Sondhi, Jennifer A. Prescher* and Michelle A. Digman*,

{"title":"Monitoring Macrophage Polarization with Gene Expression Reporters and Bioluminescence Phasor Analysis","authors":"Giulia Tedeschi, Mariana X. Navarro, Lorenzo Scipioni, Tanvi K. Sondhi, Jennifer A. Prescher* and Michelle A. Digman*, ","doi":"10.1021/cbmi.4c0004910.1021/cbmi.4c00049","DOIUrl":"https://doi.org/10.1021/cbmi.4c00049https://doi.org/10.1021/cbmi.4c00049","url":null,"abstract":"Macrophages exhibit a spectrum of behaviors upon activation and are generally classified as one of two types: inflammatory (M1) or anti-inflammatory (M2). Tracking these phenotypes in living cells can provide insight into immune function but remains a challenging pursuit. Existing methods are mostly limited to static readouts or are difficult to employ for multiplexed imaging in complex 3D environments while maintaining cellular resolution. We aimed to fill this void using bioluminescent technologies. Here we report genetically engineered luciferase reporters for the long-term monitoring of macrophage polarization via spectral phasor analysis. M1- and M2-specific promoters were used to drive the expression of bioluminescent enzymes in macrophage cell lines. The readouts were multiplexed and discernible in both 2D and 3D formats with single-cell resolution in living samples. Collectively, this work expands the toolbox of methods for monitoring macrophage polarization and provides a blueprint for monitoring other multifaceted networks in heterogeneous environments.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 11","pages":"765–774 765–774"},"PeriodicalIF":0.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142694514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid and Large-Scale Synthesis of Chiral and Fluorescent Sulfur Quantum Dots for Intracellular Temperature Monitoring 用于胞内温度监测的手性荧光硫量子点的快速大规模合成

Chemical & Biomedical Imaging Pub Date : 2024-09-20 DOI: 10.1021/cbmi.4c0005210.1021/cbmi.4c00052

Li Zhao, Tianjian Sha, Yufu Liu, Qingsong Mei*, Haibin Li, Pinghua Sun, Haibo Zhou* and Huaihong Cai*,

{"title":"Rapid and Large-Scale Synthesis of Chiral and Fluorescent Sulfur Quantum Dots for Intracellular Temperature Monitoring","authors":"Li Zhao, Tianjian Sha, Yufu Liu, Qingsong Mei*, Haibin Li, Pinghua Sun, Haibo Zhou* and Huaihong Cai*, ","doi":"10.1021/cbmi.4c0005210.1021/cbmi.4c00052","DOIUrl":"https://doi.org/10.1021/cbmi.4c00052https://doi.org/10.1021/cbmi.4c00052","url":null,"abstract":"The large-scale preparation of fluorescent nanomaterials with laboratory-relevant chemical and optical properties will greatly forward their consumer market applications; however, it still remains challenging. In this work, a universal strategy was developed for the rapid and large-scale synthesis of fluorescent sulfur quantum dots that recently has drawn great attention because of their unique optical characteristics. From the fact that empty 3d orbitals of sulfide species are able to bind with lone-pair π electrons of the heteroatomic groups, many amino-group containing compounds, such as amino acid and polyethylenimine molecules, were exploited to synthesize sulfur quantum dots. This 10 min preparation period endowed sulfur quantum dots with bright blue fluorescence and also chirality. Due to the user-friendly and rapid operation, this strategy can be extended to the large-scale synthesis of sulfur quantum dots with a yield of 16.844 g for one batch of experiment. Moreover, it was found that the sulfur quantum dots exhibited a reversible temperature-dependent luminescent property with a sensitivity of 0.72%/°C, which showed excellent intracellular temperature monitoring capability for inflammation-related disease diagnostics.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 12","pages":"817–824 817–824"},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142874938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid and Large-Scale Synthesis of Chiral and Fluorescent Sulfur Quantum Dots for Intracellular Temperature Monitoring. 用于胞内温度监测的手性荧光硫量子点的快速大规模合成。

Chemical & Biomedical Imaging Pub Date : 2024-09-20 eCollection Date: 2024-12-23 DOI: 10.1021/cbmi.4c00052

Li Zhao, Tianjian Sha, Yufu Liu, Qingsong Mei, Haibin Li, Pinghua Sun, Haibo Zhou, Huaihong Cai

{"title":"Rapid and Large-Scale Synthesis of Chiral and Fluorescent Sulfur Quantum Dots for Intracellular Temperature Monitoring.","authors":"Li Zhao, Tianjian Sha, Yufu Liu, Qingsong Mei, Haibin Li, Pinghua Sun, Haibo Zhou, Huaihong Cai","doi":"10.1021/cbmi.4c00052","DOIUrl":"10.1021/cbmi.4c00052","url":null,"abstract":"The large-scale preparation of fluorescent nanomaterials with laboratory-relevant chemical and optical properties will greatly forward their consumer market applications; however, it still remains challenging. In this work, a universal strategy was developed for the rapid and large-scale synthesis of fluorescent sulfur quantum dots that recently has drawn great attention because of their unique optical characteristics. From the fact that empty 3d orbitals of sulfide species are able to bind with lone-pair π electrons of the heteroatomic groups, many amino-group containing compounds, such as amino acid and polyethylenimine molecules, were exploited to synthesize sulfur quantum dots. This 10 min preparation period endowed sulfur quantum dots with bright blue fluorescence and also chirality. Due to the user-friendly and rapid operation, this strategy can be extended to the large-scale synthesis of sulfur quantum dots with a yield of 16.844 g for one batch of experiment. Moreover, it was found that the sulfur quantum dots exhibited a reversible temperature-dependent luminescent property with a sensitivity of 0.72%/°C, which showed excellent intracellular temperature monitoring capability for inflammation-related disease diagnostics.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 12","pages":"817-824"},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11673185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142904044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescent Water-Soluble Polycationic Chitosan Polymers as Markers for Biological 3D Imaging 作为生物三维成像标记的荧光水溶性聚阳离子壳聚糖聚合物

Chemical & Biomedical Imaging Pub Date : 2024-09-10 DOI: 10.1021/cbmi.4c0002810.1021/cbmi.4c00028

Srishti Vajpayee, Tiziana Picascia, Fabio Casciano, Elisabetta Viale, Luca Ronda, Stefano Bettati, Daniela Milani, Norbert Gretz and Rossana Perciaccante*,

{"title":"Fluorescent Water-Soluble Polycationic Chitosan Polymers as Markers for Biological 3D Imaging","authors":"Srishti Vajpayee, Tiziana Picascia, Fabio Casciano, Elisabetta Viale, Luca Ronda, Stefano Bettati, Daniela Milani, Norbert Gretz and Rossana Perciaccante*, ","doi":"10.1021/cbmi.4c0002810.1021/cbmi.4c00028","DOIUrl":"https://doi.org/10.1021/cbmi.4c00028https://doi.org/10.1021/cbmi.4c00028","url":null,"abstract":"Over the last decades, various tissue-clearing techniques have broken the ground for the optical imaging of whole organs and whole-organisms, providing complete representative data sets of three-dimensional biological structures. Along with advancements in this field, the development of fluorescent markers for staining vessels and capillaries has offered insights into the complexity of vascular networks and their impact on disease progression. Here we describe the use of a modified water-soluble chitosan linked to cyanine dyes in combination with ethyl cinnamate-based optical tissue clearing for the 3D visualization of tissue vasculature in depth. The water-soluble fluorescent Chitosans have proven to be an optimal candidate for labeling both vessels and capillaries ex vivo thanks to their increased water solubility, high photostability, and optical properties in the near-infrared window. In addition, the nontoxicity of these markers broadens their applicability to in vitro and in vivo biological applications. Despite the availability of other fluorescent molecules for vascular staining, the present study, for the first time, demonstrates the potential of fluorescent chitosans to depict vessels at the capillary level and opens avenues for advancing the diagnostic field by reducing the complexity and costs of the currently available procedures.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 10","pages":"721–730 721–730"},"PeriodicalIF":0.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142550365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescent Water-Soluble Polycationic Chitosan Polymers as Markers for Biological 3D Imaging. 作为生物三维成像标记的荧光水溶性多阳离子壳聚糖聚合物。

Chemical & Biomedical Imaging Pub Date : 2024-09-10 eCollection Date: 2024-10-28 DOI: 10.1021/cbmi.4c00028

Srishti Vajpayee, Tiziana Picascia, Fabio Casciano, Elisabetta Viale, Luca Ronda, Stefano Bettati, Daniela Milani, Norbert Gretz, Rossana Perciaccante

{"title":"Fluorescent Water-Soluble Polycationic Chitosan Polymers as Markers for Biological 3D Imaging.","authors":"Srishti Vajpayee, Tiziana Picascia, Fabio Casciano, Elisabetta Viale, Luca Ronda, Stefano Bettati, Daniela Milani, Norbert Gretz, Rossana Perciaccante","doi":"10.1021/cbmi.4c00028","DOIUrl":"10.1021/cbmi.4c00028","url":null,"abstract":"Over the last decades, various tissue-clearing techniques have broken the ground for the optical imaging of whole organs and whole-organisms, providing complete representative data sets of three-dimensional biological structures. Along with advancements in this field, the development of fluorescent markers for staining vessels and capillaries has offered insights into the complexity of vascular networks and their impact on disease progression. Here we describe the use of a modified water-soluble chitosan linked to cyanine dyes in combination with ethyl cinnamate-based optical tissue clearing for the 3D visualization of tissue vasculature in depth. The water-soluble fluorescent Chitosans have proven to be an optimal candidate for labeling both vessels and capillaries ex vivo thanks to their increased water solubility, high photostability, and optical properties in the near-infrared window. In addition, the nontoxicity of these markers broadens their applicability to in vitro and in vivo biological applications. Despite the availability of other fluorescent molecules for vascular staining, the present study, for the first time, demonstrates the potential of fluorescent chitosans to depict vessels at the capillary level and opens avenues for advancing the diagnostic field by reducing the complexity and costs of the currently available procedures.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 10","pages":"721-730"},"PeriodicalIF":0.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11522997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142559439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precision Imaging of Biothiols in Live Cells and Treatment Evaluation during the Development of Liver Injury via a Near-Infrared Fluorescent Probe. 近红外荧光探针在肝损伤发展过程中对活细胞生物硫醇的精确成像及治疗评价。

Chemical & Biomedical Imaging Pub Date : 2024-08-28 eCollection Date: 2025-03-24 DOI: 10.1021/cbmi.4c00048

Yinshuang Yang, Xiaolan Liu, Deyang Xi, Yibin Zhang, Xiucai Gao, Kai Xu, Haiying Liu, Mingxi Fang

引用次数: 0

Precision Imaging of Biothiols in Live Cells and Treatment Evaluation during the Development of Liver Injury via a Near-Infrared Fluorescent Probe 近红外荧光探针在肝损伤发展过程中对活细胞生物硫醇的精确成像及治疗评价

Chemical & Biomedical Imaging Pub Date : 2024-08-28 DOI: 10.1021/cbmi.4c0004810.1021/cbmi.4c00048

Yinshuang Yang, Xiaolan Liu, Deyang Xi, Yibin Zhang, Xiucai Gao, Kai Xu*, Haiying Liu* and Mingxi Fang*,

引用次数: 0

Peptide PET Imaging: A Review of Recent Developments and a Look at the Future of Radiometal-Labeled Peptides in Medicine. 多肽 PET 成像：多肽正电子发射计算机断层成像：最新发展综述及放射性同位素标记多肽在医学中的未来展望》（Peptide PET Imaging: A Review of Recent Developments and a Look at the Future of Radiometal-Labeled Peptides in Medicine.

Chemical & Biomedical Imaging Pub Date : 2024-08-23 eCollection Date: 2024-09-23 DOI: 10.1021/cbmi.4c00030

Majed Shabsigh, Lee A Solomon

引用次数: 0