Chemical & Biomedical Imaging最新文献

{"title":"An Activatable Semiconducting Nanoprobe for Early and Accurate Diagnosis of Liver Injury.","authors":"Fei Li, Shaobin Wu, Keyang Li, Jun Zhu, Shasha He, Huayu Tian","doi":"10.1021/cbmi.4c00022","DOIUrl":"https://doi.org/10.1021/cbmi.4c00022","url":null,"abstract":"<p><p>Liver injury, caused by factors like viral hepatitis and drug overdose, poses a significant health risk, with current diagnostic methods lacking specificity, increasing the need for more precise molecular imaging techniques. Herein, we present an activatable semiconducting liver injury reporter (SLIR) for early and accurate diagnosis of liver injury. The SLIR, which is composed of semiconducting polymers with an electron-withdrawing quenching segment, remains nonfluorescent until it encounters biothiols such as cysteine in the liver. SLIR accumulates efficiently in the liver and respond rapidly to biothiols, allowing accurate and early detection of liver damage. The recovery of SLIR fluorescence negatively reflects the dynamics of oxidative stress in the liver and provides information on the severity of tissue damage. Thus, the specificity of SLIR, the fast response, and the efficient targeting of the liver make it a promising tool for the precise diagnosis of liver damage at an early stage.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 8","pages":"569-576"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11503912/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142548908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Activatable Semiconducting Nanoprobe for Early and Accurate Diagnosis of Liver Injury","authors":"Fei Li,&nbsp;Shaobin Wu,&nbsp;Keyang Li,&nbsp;Jun Zhu,&nbsp;Shasha He* and Huayu Tian*,&nbsp;","doi":"10.1021/cbmi.4c0002210.1021/cbmi.4c00022","DOIUrl":"https://doi.org/10.1021/cbmi.4c00022https://doi.org/10.1021/cbmi.4c00022","url":null,"abstract":"<p >Liver injury, caused by factors like viral hepatitis and drug overdose, poses a significant health risk, with current diagnostic methods lacking specificity, increasing the need for more precise molecular imaging techniques. Herein, we present an activatable semiconducting liver injury reporter (SLIR) for early and accurate diagnosis of liver injury. The SLIR, which is composed of semiconducting polymers with an electron-withdrawing quenching segment, remains nonfluorescent until it encounters biothiols such as cysteine in the liver. SLIR accumulates efficiently in the liver and respond rapidly to biothiols, allowing accurate and early detection of liver damage. The recovery of SLIR fluorescence negatively reflects the dynamics of oxidative stress in the liver and provides information on the severity of tissue damage. Thus, the specificity of SLIR, the fast response, and the efficient targeting of the liver make it a promising tool for the precise diagnosis of liver damage at an early stage.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 8","pages":"569–576 569–576"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142075408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visible Tracking of Small Molecules of Gases with Fluorescent Donors","authors":"Jing Yu,&nbsp;Jie Xu,&nbsp;Siyue Ma,&nbsp;Chao Wang,&nbsp;Qing Miao,&nbsp;Linlin Wang and Guang Chen*,&nbsp;","doi":"10.1021/cbmi.4c00006","DOIUrl":"10.1021/cbmi.4c00006","url":null,"abstract":"<p >Biological gasotransmitters (small molecules of gases) play important roles in signal transduction mechanisms and disease treatments. Although a large number of small-molecule donors have been developed, visualizing the release of small molecules remains challenging. Owing to their unique optical properties, fluorophores have been widely applied in cellular imaging and tracking. Researchers have used various fluorophores to develop small-molecule donors with fluorescent activity for visualizing the release of small molecules and their related therapies. These include fluorophores and their derivatives such as boron-dipyrromethene (BODIPY), coumarin, 1,8-naphthalimide, hemicyanine, porphyrin, rhodamine, and fluorescein. In this review, we summarize the design concepts of functional fluorescent small-molecule donors in terms of different types of fluorophores. Then, we discuss how these donors release small molecules, and the imaging modalities and biomedical applications facilitated by their fluorescent properties. With the systematic discussion of these publications, we hope to provide useful references for the development of more practical, advanced fluorescent small-molecule donors in the future.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 6","pages":"401–412"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140784897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoscale Chemical Probing of Metal-Supported Ultrathin Ferrous Oxide via Tip-Enhanced Raman Spectroscopy and Scanning Tunneling Microscopy","authors":"Dairong Liu,&nbsp;Linfei Li and Nan Jiang*,&nbsp;","doi":"10.1021/cbmi.4c00015","DOIUrl":"10.1021/cbmi.4c00015","url":null,"abstract":"<p >Metal-supported ultrathin ferrous oxide (FeO) has attracted immense interest in academia and industry due to its widespread applications in heterogeneous catalysis. However, chemical insight into the local structural characteristics of FeO, despite its critical importance in elucidating structure–property relationships, remains elusive. In this work, we report the nanoscale chemical probing of gold (Au)-supported ultrathin FeO via ultrahigh-vacuum tip-enhanced Raman spectroscopy (UHV-TERS) and scanning tunneling microscopy (STM). For comparative analysis, single-crystal Au(111) and Au(100) substrates are used to tune the interfacial properties of FeO. Although STM images show distinctly different moiré superstructures on FeO nanoislands on Au(111) and Au(100), TERS demonstrates the same chemical nature of FeO by comparable vibrational features. In addition, combined TERS and STM measurements identify a unique wrinkled FeO structure on Au(100), which is correlated to the reassembly of the intrinsic Au(100) surface reconstruction due to FeO deposition. Beyond revealing the morphologies of ultrathin FeO on Au substrates, our study provides a thorough understanding of the local interfacial properties and interactions of FeO on Au, which could shed light on the rational design of metal-supported FeO catalysts. Furthermore, this work demonstrates the promising utility of combined TERS and STM in chemically probing the structural properties of metal-supported ultrathin oxides on the nanoscale.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 5","pages":"345–351"},"PeriodicalIF":0.0,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140221792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing Multichannel smFRET Approach to Dissecting Ribosomal Mechanisms","authors":"Ran Lin,&nbsp; and ,&nbsp;Yuhong Wang*,&nbsp;","doi":"10.1021/cbmi.4c0001010.1021/cbmi.4c00010","DOIUrl":"https://doi.org/10.1021/cbmi.4c00010https://doi.org/10.1021/cbmi.4c00010","url":null,"abstract":"<p >The ribosome, a 2.6 megadalton biomolecule measuring approximately 20 nm in diameter, coordinates numerous ligands, factors, and regulators to translate proteins with high fidelity and speed. Understanding its complex functions necessitates multiperspective observations. We developed a dual-FRET single-molecule Förste Resonance Energy Transfer method (dual-smFRET), allowing simultaneous observation and correlation of tRNA dynamics and Elongation Factor G (EF-G) conformations in the same complex, in a 10 s time window. By synchronizing laser shutters and motorized filter sets, two FRET signals are captured in consecutive 5 s intervals with a time gap of 50–100 ms. We observed distinct fluorescent emissions from single-, double-, and quadruple-labeled ribosome complexes. Through comprehensive spectrum analysis and correction, we distinguish and correlate conformational changes in two parts of the ribosome, offering additional perspectives on its coordination and timing during translocation. Our setup’s versatility, accommodating up to six FRET pairs, suggests broader applications in studying large biomolecules and various biological systems.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 7","pages":"501–509 501–509"},"PeriodicalIF":0.0,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141959304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimal Sparse Energy Sampling for X-ray Spectro-Microscopy: Reducing the X-ray Dose and Experiment Time Using Model Order Reduction","authors":"Paul D. Quinn*,&nbsp;Malena Sabaté Landman,&nbsp;Tom Davis,&nbsp;Melina Freitag,&nbsp;Silvia Gazzola and Sergey Dolgov,&nbsp;","doi":"10.1021/cbmi.3c00116","DOIUrl":"10.1021/cbmi.3c00116","url":null,"abstract":"<p >The application of X-ray spectro-microscopy to image changes in the chemical state in application areas such as catalysis, environmental science, or biological samples can be limited by factors such as the speed of measurement, the presence of dilute concentrations, radiation damage, and thermal drift during the measurement. We have adapted a reduced-order model approach, known as the discrete empirical interpolation method, which identifies how to optimally subsample the spectroscopic information, accounting for background variations in the signal, to provide an accurate approximation of an equivalent full spectroscopic measurement from the sampled material. This approach uses readily available prior information to guide and significantly reduce the sampling requirements impacting both the total X-ray dose and the acquisition time. The reduced-order model approach can be adapted more broadly to any spectral or spectro-microscopy measurement where a low-rank approximation can be made from prior information on the possible states of a system, and examples of the approach are presented.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 4","pages":"283–292"},"PeriodicalIF":0.0,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.3c00116","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140228688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimation of Medium Depolarization Index with Noise Immunity in Catheter-Based PS-OCT toward Vascular Plaques Detection","authors":"Qingrui Li,&nbsp;Zhenyang Ding*,&nbsp;Yongle Li*,&nbsp;Kun Liu,&nbsp;Rongyang Zhu,&nbsp;Junfeng Jiang and Tiegen Liu,&nbsp;","doi":"10.1021/cbmi.3c00119","DOIUrl":"10.1021/cbmi.3c00119","url":null,"abstract":"<p >Medium depolarization imaging by catheter-based polarization-sensitive optical coherence tomography (PS-OCT) can provide valuable insight into significant features of lipid, macrophages, and cholesterol crystals in atherosclerotic vulnerable plaques. In this paper, we demonstrate a method to achieve an accurate estimation of the medium depolarization index (EMDI) with noise immunity in catheter-based PS-OCT. EMDI is calculated by an iterative approximation based on Lu–Chipman matrix decomposition and Frobenius norm judgment of incoherent averaging of Mueller matrices. Monte Carlo simulation results verify that the medium depolarization measurement by EMDI is 3.3 times more accurate compared with those of the depolarization index (DI) and degree of polarization uniformity (DOPU). In experiments, we design a microsphere suspension with various concentrations and measure EMDI under different additive noise. Consistently, the measurement accuracy by EMDI is increased 2.85 times compared to those by DI and DOPU. For vascular plaques detection, we use protein and cholesterol gel as plaque phantoms. Based on PS-OCT images of plaque phantom in vitro and in ex vivo porcine coronary artery, the recognition rate of plaque by EMDI is 2.99 to 4.65 times higher than those by DI and DOPU evaluated by spatial response of the Laplacian operator (SRLO).</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 4","pages":"293–303"},"PeriodicalIF":0.0,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.3c00119","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140243663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The DIAD Approach to Correlative Synchrotron X-ray Imaging and Diffraction Analysis of Human Enamel","authors":"Cyril Besnard*,&nbsp;Ali Marie,&nbsp;Sisini Sasidharan,&nbsp;Hans Deyhle,&nbsp;Andrew M. James,&nbsp;Sharif I. Ahmed,&nbsp;Christina Reinhard,&nbsp;Robert A. Harper,&nbsp;Richard M. Shelton,&nbsp;Gabriel Landini and Alexander M. Korsunsky*,&nbsp;","doi":"10.1021/cbmi.3c00122","DOIUrl":"10.1021/cbmi.3c00122","url":null,"abstract":"<p >The Dual Imaging and Diffraction (DIAD) beamline at Diamond Light Source (Didcot, U.K.) implements a correlative approach to the dynamic study of materials based on concurrent analysis of identical sample locations using complementary X-ray modalities to reveal structural detail at various length scales. Namely, the underlying beamline principle and its practical implementation allow the collocation of chosen regions within the sample and their interrogation using real-space imaging (radiography and tomography) and reciprocal space scattering (diffraction). The switching between the two principal modes is made smooth and rapid by design, so that the data collected is interlaced to obtain near-simultaneous multimodal characterization. Different specific photon energies are used for each mode, and the interlacing of acquisition steps allows conducting static and dynamic experiments. Building on the demonstrated realization of this state-of-the-art approach requires further refining of the experimental practice, namely, the methods for gauge volume collocation under different modes of beam–sample interaction. To address this challenge, experiments were conducted at DIAD devoted to the study of human dental enamel, a hierarchical structure composed of hydroxyapatite mineral nanocrystals, as a static sample previously affected by dental caries (tooth decay) as well as under dynamic conditions simulating the process of acid demineralization. Collocation and correlation were achieved between WAXS (wide-angle X-ray scattering), 2D (radiographic), and 3D (tomographic) imaging. While X-ray imaging in 2D or 3D modes reveals real-space details of the sample microstructure, X-ray scattering data for each gauge volume provided statistical nanoscale and ultrastructural polycrystal reciprocal-space information such as phase and preferred orientation (texture). Careful registration of the gauge volume positions recorded during the scans allowed direct covisualization of the data from two modalities. Diffraction gauge volumes were identified and visualized within the tomographic data sets, revealing the underlying local information to support the interpretation of the diffraction patterns. The present implementation of the 4D microscopy paradigm allowed following the progression of demineralization and its correlation with time-dependent WAXS pattern evolution in an approach that is transferable to other material systems.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 3","pages":"222–232"},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.3c00122","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140076927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensing Mechanism of Cysteine Specific Fluorescence Probes and Their Application of Cysteine Recognition","authors":"Rizwana Asghar,&nbsp;Yongchuang Li,&nbsp;Fangjun Huo* and Caixia Yin*,&nbsp;","doi":"10.1021/cbmi.4c00001","DOIUrl":"10.1021/cbmi.4c00001","url":null,"abstract":"<p >Due to the biological importance of cysteine (Cys), the development of organic fluorescence probes for Cys has been a wide, potent, and outstanding research field in most recent years. It has been used as a biomarker in treating various diseases; therefore, developing a sensing mechanism for detecting Cys is very important. In this Review, we focus on and summarize the specific results of recent exciting literature regarding the sensing mechanism of Cys-specific fluorescence probes and their applications in Cys recognition. Moreover, a design strategy of the sensing mechanism of Cys can be classified into seven reaction mechanisms, including the aromatic substitution rearrangement reaction, cyclization of aldehyde, Michael addition reaction, Se–N or S–S or bond cleavage reaction, addition cyclization of acrylate, metal complex reaction, and nucleophilic substitution reaction. In all sections, discussions have corresponded to Cys-specific sensing mechanisms, which consist of emission, color changes, and detection limits and deal with the application and recognition sites of molecules. Future directions and challenges have been proposed for the preparation of Cys-specific probes.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 4","pages":"250–269"},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140422336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A NIR Dual-Channel Fluorescent Probe for Fluctuations of Intracellular Polarity and H2O2 and Its Applications for the Visualization of Inflammation and Ferroptosis","authors":"Xing Tian,&nbsp;Jiayi Cheng,&nbsp;Lei Yang*,&nbsp;Zhanxian Li* and Mingming Yu*,&nbsp;","doi":"10.1021/cbmi.3c0012310.1021/cbmi.3c00123","DOIUrl":"https://doi.org/10.1021/cbmi.3c00123https://doi.org/10.1021/cbmi.3c00123","url":null,"abstract":"<p >Hydrogen peroxide and polarity are closely related to many physiological activities and pathological processes. However, near-infrared fluorescent probes that are sensitive to both H₂O₂ and polarity are still scarce. Herein, we developed the first dual-channel near-infrared fluorescent probe NBO, with an AIE effect, enabling simultaneous monitoring of H₂O₂ and polarity. The probe presented high sensitivity, high selectivity, and low detection limit for H₂O₂. It also had high sensitivity to polarity, independent of pH and viscosity, with large Stokes shifts, good photostability, and low cytotoxicity. Moreover, NBO was able to detect both endogenous and exogenous H₂O₂ as well as polarity fluctuations <i>in vivo</i> as a method to effectively differentiate between cancer cells and normal cells. Importantly, it also could monitor the therapeutic effects of drugs in inflammation and iron-dead cells and mice. Based on NIR emission, NBO could be used as an imaging tool and a way to evaluate the therapeutic effect of drugs for inflammation and ferroptosis.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 7","pages":"518–525 518–525"},"PeriodicalIF":0.0,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.3c00123","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141955317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}