Chemical & Biomedical Imaging最新文献

Honoring the Contributions of Our Editorial Board Members 表彰编委会成员的贡献

Chemical & Biomedical Imaging Pub Date : 2025-04-15 DOI: 10.1021/cbmi.5c0002610.1021/cbmi.5c00026

Wenxi Lei, Deju Ye and Zijian Guo*,

引用次数: 0

Positron Emission Tomography Imaging of the Adaptive Immune System in Cardiovascular Diseases 心血管疾病中适应性免疫系统的正电子发射断层成像

Chemical & Biomedical Imaging Pub Date : 2025-03-18 DOI: 10.1021/cbmi.4c0011710.1021/cbmi.4c00117

Jaume Ramon Otaegui, Deborah Sultan, Gyu Seong Heo and Yongjian Liu*,

{"title":"Positron Emission Tomography Imaging of the Adaptive Immune System in Cardiovascular Diseases","authors":"Jaume Ramon Otaegui, Deborah Sultan, Gyu Seong Heo and Yongjian Liu*, ","doi":"10.1021/cbmi.4c0011710.1021/cbmi.4c00117","DOIUrl":"https://doi.org/10.1021/cbmi.4c00117https://doi.org/10.1021/cbmi.4c00117","url":null,"abstract":"Cardiovascular diseases are the leading cause of death around the globe. In recent years, a crucial role of the immune system has been acknowledged in cardiac disease progression, opening the door for immunomodulatory therapies. To this ongoing change of paradigm, positron emission tomography (PET) imaging of the immune system has become a remarkable tool to reveal immune cell trafficking and monitor disease progression and treatment response. Currently, PET imaging of the immune system in cardiovascular disease mainly focuses on the innate immune system such as macrophages, while the immune cells of the adaptive immune system including B and T cells are less studied. This can be ascribed to the lack of radiotracers specifically binding to B and T cell biomarkers compatible with PET imaging within the cardiovascular system. In this review, we summarize current knowledge about the role of the adaptive immune system (e.g., B and T cells) in major cardiovascular diseases and introduce key biomarkers for specific targeting of these immune cells and their subpopulations. Finally, we present available radiotracers for these biomarkers and propose a pathway for developing probes or optimizing those already used in other fields (e.g., oncology) to make them compatible with the cardiovascular system.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 4","pages":"209–224 209–224"},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00117","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Droplet Wettability on Scanning Electrochemical Cell Microscopy Performance in Stainless Steels 液滴润湿性对不锈钢扫描电化学显微镜性能的影响

Chemical & Biomedical Imaging Pub Date : 2025-03-13 DOI: 10.1021/cbmi.4c0010110.1021/cbmi.4c00101

Sarah R. Yassine, León Zendejas Medina, Egor Katkov, Robert Lacasse and Janine Mauzeroll*,

引用次数: 0

A Self-Immobilizing Photoacoustic Probe for Ratiometric In Vivo Imaging of Cu(II) in Tumors 一种用于肿瘤中Cu（II）体内比例成像的自固定光声探针

Chemical & Biomedical Imaging Pub Date : 2025-03-03 DOI: 10.1021/cbmi.4c0011510.1021/cbmi.4c00115

Qian Sun, Hang Liu, Ying Yang, Shankun Yao, Zhipeng Liu* and Zijian Guo*,

{"title":"A Self-Immobilizing Photoacoustic Probe for Ratiometric In Vivo Imaging of Cu(II) in Tumors","authors":"Qian Sun, Hang Liu, Ying Yang, Shankun Yao, Zhipeng Liu* and Zijian Guo*, ","doi":"10.1021/cbmi.4c0011510.1021/cbmi.4c00115","DOIUrl":"https://doi.org/10.1021/cbmi.4c00115https://doi.org/10.1021/cbmi.4c00115","url":null,"abstract":"Cu(II) ions play a critical role in tumor growth and metastasis, making in vivo high-resolution imaging of Cu(II) crucial for understanding its role in tumor pathophysiology. However, designing suitable molecular probes for this purpose remains challenging. Herein, we report the development of a photoacoustic probe for specific in vivo imaging of Cu(II) in tumors. This probe utilizes β-galactoside as a targeting group and incorporates a unique self-immobilization strategy. Upon β-galactosidase-mediated cleavage, the probe generates a reactive quinone methide intermediate that covalently binds to intracellular proteins, enabling selective tumor accumulation. The probe exhibits a ratiometric photoacoustic response to Cu(II) with high selectivity over that of other biological species. In vitro and in vivo studies demonstrated the efficacy of the probe for Cu(II) imaging in tumors. This research provides valuable insights into the role of Cu(II) in tumorigenesis and may facilitate the development of diagnostic and therapeutic approaches for cancer.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 4","pages":"260–266 260–266"},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorogenic Linkage Integration for Nonfluorescent Transformations (FLINT) 非荧光转化的荧光连锁集成（FLINT）

Chemical & Biomedical Imaging Pub Date : 2025-03-03 DOI: 10.1021/cbmi.4c0011410.1021/cbmi.4c00114

Bishal Pokhrel, Fatiha Farhana, Li Zuo, Rebecca L. Stratton, Pravin Pokhrel, Mohammad Akter Hossain, Jiahao Ji, Hanbin Mao and Hao Shen*,

{"title":"Fluorogenic Linkage Integration for Nonfluorescent Transformations (FLINT)","authors":"Bishal Pokhrel, Fatiha Farhana, Li Zuo, Rebecca L. Stratton, Pravin Pokhrel, Mohammad Akter Hossain, Jiahao Ji, Hanbin Mao and Hao Shen*, ","doi":"10.1021/cbmi.4c0011410.1021/cbmi.4c00114","DOIUrl":"https://doi.org/10.1021/cbmi.4c00114https://doi.org/10.1021/cbmi.4c00114","url":null,"abstract":"Since its creation, single-molecule optical imaging has revolutionized the study of catalytic processes, yet its application largely relies on probing fluorogenic reactions. To overcome this limitation, we propose the Fluorogenic Linkage Integration for Nonfluorescent Transformation (FLINT) approach, an imaging method to resolve nonfluorogenic reactions at the single-molecule level. Using glucose oxidation as a model reaction, we coupled this nonfluorogenic reaction with a fluorogenic Amplex Red (AR) → resorufin (RF) transformation to create a cascading reaction. This integration allowed us to monitor single-turnover events and extract key kinetic parameters for glucose oxidation despite their being invisible under the optical microscope. Our ensemble measurements combining cyclic voltammetry and fluorescence spectroscopy confirmed the cascade reaction mechanism and revealed first-order kinetics for both elementary reaction steps. At the single-molecule level, turnover time analysis provided detailed information on the reaction kinetics, distinguishing the relatively fast glucose oxidation from slower AR oxidation. We further confirmed the validity of the FLINT approach by comparing the catalytic performances of 5 nm gold nanoparticles (AuNPs) against that of 18 × 52 nm gold nanorods (AuNRs) and AuNP@DNA coronazymes. Furthermore, FLINT was used to evaluate the chiral selectivity of d- and l-glucose on coronazymes, suggesting the potential application of FLINT in enantioselective reactions. The FLINT approach is a significant advancement in single-molecule imaging as it enables the study of nonfluorogenic reactions with high spatiotemporal resolution.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 4","pages":"253–259 253–259"},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and Characterization of a Novel Photocleavable Fluorescent Dye Dyad for Diffusion Imaging 一种新型光可切割扩散成像荧光染料的合成与表征

Chemical & Biomedical Imaging Pub Date : 2025-02-21 DOI: 10.1021/cbmi.4c0008410.1021/cbmi.4c00084

Damian Schöngen, and , Dominik Wöll*,

{"title":"Synthesis and Characterization of a Novel Photocleavable Fluorescent Dye Dyad for Diffusion Imaging","authors":"Damian Schöngen,  and , Dominik Wöll*, ","doi":"10.1021/cbmi.4c0008410.1021/cbmi.4c00084","DOIUrl":"https://doi.org/10.1021/cbmi.4c00084https://doi.org/10.1021/cbmi.4c00084","url":null,"abstract":"We report the synthesis and characterization of a photocleavable fluorescent dye dyad. The two constituting dyes show a large spectral overlap and are in close proximity to each other, leading to efficient Förster Resonance Energy Transfer (FRET). Photocleavage of the dyad and the subsequent independent diffusion of both fluorophores qualifies the system to be used for high accuracy diffusion measurements. In contrast to previous work, the dyad reported here can be applied in polar solvents and cleaved by UV-A light. Beneficially, the photolabile linker provides two orthogonal labeling sites for various commercially available fluorescent labels. In this work, we chose the cationic organic dyes ATTO565 and ATTO647N. We outline the synthesis and spectral characterization of the system with UV–Vis and fluorescence spectroscopy as well as fluorescence lifetime and fluorescence quantum yield measurements. Furthermore, we performed proof-of-principle microscopy experiments to demonstrate its capability in polyvinyl acetate films.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 3","pages":"199–207 199–207"},"PeriodicalIF":0.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00084","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143675804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and Characterization of a Novel Photocleavable Fluorescent Dye Dyad for Diffusion Imaging. 一种新型光可切割扩散成像荧光染料的合成与表征。

Chemical & Biomedical Imaging Pub Date : 2025-02-21 eCollection Date: 2025-03-24 DOI: 10.1021/cbmi.4c00084

Damian Schöngen, Dominik Wöll

引用次数: 0

Label-Free Visualization and Segmentation of Endothelial Cell Mitochondria Using Holotomographic Microscopy and U-Net 利用全息层析显微镜和U-Net技术对内皮细胞线粒体进行无标记可视化和分割

Chemical & Biomedical Imaging Pub Date : 2025-02-18 DOI: 10.1021/cbmi.4c0010010.1021/cbmi.4c00100

Raul Michael, Tallah Modirzadeh, Tahir Bachar Issa and Patrick Jurney*,

{"title":"Label-Free Visualization and Segmentation of Endothelial Cell Mitochondria Using Holotomographic Microscopy and U-Net","authors":"Raul Michael, Tallah Modirzadeh, Tahir Bachar Issa and Patrick Jurney*, ","doi":"10.1021/cbmi.4c0010010.1021/cbmi.4c00100","DOIUrl":"https://doi.org/10.1021/cbmi.4c00100https://doi.org/10.1021/cbmi.4c00100","url":null,"abstract":"Understanding the physiological processes underlying cardiovascular disease (CVD) requires examination of endothelial cell (EC) mitochondrial networks, because mitochondrial function and adenosine triphosphate production are crucial in EC metabolism, and consequently influence CVD progression. Although current biochemical assays and immunofluorescence microscopy can reveal how mitochondrial function influences cellular metabolism, they cannot achieve live observation and tracking changes in mitochondrial networks through fusion and fission events. Holotomographic microscopy (HTM) has emerged as a promising technique for real-time, label-free visualization of ECs and their organelles, such as mitochondria. This nondestructive, noninterfering live cell imaging method offers unprecedented opportunities to observe mitochondrial network dynamics. However, because existing image processing tools based on immunofluorescence microscopy techniques are incompatible with HTM images, a machine-learning model is required. Here, we developed a model using a U-net learner with a Resnet18 encoder to identify four classes within HTM images: mitochondrial networks, cell borders, ECs, and background. This method accurately identifies mitochondrial structures and positions. With high accuracy and similarity metrics, the output image successfully provides visualization of mitochondrial networks within HTM images of ECs. This approach enables the study of mitochondrial networks and their effects, and holds promise in advancing understanding of CVD mechanisms.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 4","pages":"225–231 225–231"},"PeriodicalIF":0.0,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00100","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the Local Fate of Charge Carriers in Halide Perovskite Thin Films via Correlation Clustering Imaging 利用相关聚类成像揭示卤化物钙钛矿薄膜中载流子的局部命运

Chemical & Biomedical Imaging Pub Date : 2025-02-17 DOI: 10.1021/cbmi.4c0011310.1021/cbmi.4c00113

Sudipta Seth*, Boris Louis, Koki Asano, Toon Van Roy, Maarten B. J. Roeffaers, Elke Debroye, Ivan G. Scheblykin, Martin Vacha* and Johan Hofkens*,

{"title":"Unveiling the Local Fate of Charge Carriers in Halide Perovskite Thin Films via Correlation Clustering Imaging","authors":"Sudipta Seth*, Boris Louis, Koki Asano, Toon Van Roy, Maarten B. J. Roeffaers, Elke Debroye, Ivan G. Scheblykin, Martin Vacha* and Johan Hofkens*, ","doi":"10.1021/cbmi.4c0011310.1021/cbmi.4c00113","DOIUrl":"https://doi.org/10.1021/cbmi.4c00113https://doi.org/10.1021/cbmi.4c00113","url":null,"abstract":"As the field of metal halide perovskites matures, a range of compositionally different perovskite films has found a place in efficient optoelectronic devices. These films feature variable local structural stability, carrier diffusion, and recombination, while there is still a lack of easy-to-implement generic protocols for high-throughput characterization of these variable properties. Correlation clustering imaging (CLIM) is a recently developed tool that resolves peculiarities of local photophysics by assessing the dynamics of photoluminescence detected by wide-field optical microscopy. We demonstrate the capability of CLIM as a high-throughput characterization tool of perovskite films using MAPbI3 (MAPI) and triple cation mixed halide (TCMH) perovskites as examples where it resolves the interplay of carrier diffusion, recombination, and defect dynamics. We found significant differences in the appearance of metastable defect states in these two films. Despite a better surface quality and larger grain size, MAPI films showed more pronounced effects of fluctuating defect states than did TCMH films. As CLIM shows a significant difference between materials known to lead to different solar cell efficiencies, it can be considered a tool for quality control of thin films for perovskite optoelectronic devices.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 4","pages":"244–252 244–252"},"PeriodicalIF":0.0,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in Tracing Techniques: Mapping the Trajectory of Mesenchymal Stem-Cell-Derived Extracellular Vesicles. 追踪技术的进展：绘制间充质干细胞衍生细胞外囊泡的轨迹。

Chemical & Biomedical Imaging Pub Date : 2025-02-02 eCollection Date: 2025-03-24 DOI: 10.1021/cbmi.4c00085

Jingqi Li, Zhaoyu Wang, Yongchun Wei, Wenshuai Li, Mingzhu He, Jingjing Kang, Jia Xu, Dingbin Liu

{"title":"Advances in Tracing Techniques: Mapping the Trajectory of Mesenchymal Stem-Cell-Derived Extracellular Vesicles.","authors":"Jingqi Li, Zhaoyu Wang, Yongchun Wei, Wenshuai Li, Mingzhu He, Jingjing Kang, Jia Xu, Dingbin Liu","doi":"10.1021/cbmi.4c00085","DOIUrl":"10.1021/cbmi.4c00085","url":null,"abstract":"Mesenchymal stem-cell-derived extracellular vesicles (MSC-EVs) are nanoscale lipid bilayer vesicles secreted by mesenchymal stem cells. They inherit the parent cell's attributes, facilitating tissue repair and regeneration, promoting angiogenesis, and modulating the immune response, while offering advantages like reduced immunogenicity, straightforward administration, and enhanced stability for long-term storage. These characteristics elevate MSC-EVs as highly promising in cell-free therapy with notable clinical potential. It is critical to delve into their pharmacokinetics and thoroughly elucidate their intracellular and in vivo trajectories. A detailed summary and evaluation of existing tracing strategies are needed to establish standardized protocols. Here, we have summarized and anticipated the research progress of MSC-EVs in various biomedical imaging techniques, including fluorescence imaging, bioluminescence imaging, nuclear imaging (PET, SPECT), tomographic imaging (CT, MRI), and photoacoustic imaging. The challenges and prospects of MSC-EV tracing strategies, with particular emphasis on clinical translation, have been analyzed, with promising solutions proposed.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 3","pages":"137-168"},"PeriodicalIF":0.0,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0