Chemical & Biomedical Imaging最新文献

Honoring the Contributions of Our Editorial Board Members 表彰编委会成员的贡献

Chemical & Biomedical Imaging Pub Date : 2025-04-15 DOI: 10.1021/cbmi.5c0002610.1021/cbmi.5c00026

Wenxi Lei, Deju Ye and Zijian Guo*,

引用次数: 0

Aptamers as a New Frontier in Molecular Cancer Imaging Technologies 适体：肿瘤分子成像技术的新前沿

Chemical & Biomedical Imaging Pub Date : 2025-04-09 DOI: 10.1021/cbmi.4c0010310.1021/cbmi.4c00103

Yingying Li, Tong Shao, Jingyu Kuang, Heqing Yi, Lvyun Zhu* and Xue-Qiang Wang*,

{"title":"Aptamers as a New Frontier in Molecular Cancer Imaging Technologies","authors":"Yingying Li, Tong Shao, Jingyu Kuang, Heqing Yi, Lvyun Zhu* and Xue-Qiang Wang*, ","doi":"10.1021/cbmi.4c0010310.1021/cbmi.4c00103","DOIUrl":"https://doi.org/10.1021/cbmi.4c00103https://doi.org/10.1021/cbmi.4c00103","url":null,"abstract":"Molecular imaging has emerged as a transformative tool in cancer diagnosis, enabling the visualization of biological processes at the cellular and molecular levels. Aptamers, single-stranded oligonucleotides with high affinity and specificity for target molecules, have gained significant attention as versatile probes for molecular imaging due to their unique properties, including small size, ease of modification, low immunogenicity, and rapid tissue penetration. This review explores the integration of aptamers with various imaging agents to enhance cancer diagnosis and therapy. Aptamer-based imaging probes offer high sensitivity and real-time visualization of tumor markers. Aptamer-based fluorescence probes and aptamer-conjugated magnetic resonance imaging (MRI) probes, including gadolinium-based contrast agents, improve tumor targeting and imaging resolution. Additionally, aptamers have been utilized in single-photon emission computed tomography (SPECT) and positron emission tomography (PET) imaging to enhance the specificity of radiotracers for cancer detection. Furthermore, aptamer-targeted ultrasound and computed tomography (CT) imaging demonstrate the potential for noninvasive and precise tumor localization. By leveraging the unique advantages of aptamers, these imaging strategies not only improve diagnostic accuracy but also pave the way for image-guided cancer therapies. This review highlights the significant role of aptamers in advancing molecular imaging and their potential to revolutionize cancer diagnosis and treatment.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 5","pages":"267–279 267–279"},"PeriodicalIF":0.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00103","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144133889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Positron Emission Tomography Imaging of the Adaptive Immune System in Cardiovascular Diseases. 心血管疾病中适应性免疫系统的正电子发射断层成像。

Chemical & Biomedical Imaging Pub Date : 2025-03-19 eCollection Date: 2025-04-28 DOI: 10.1021/cbmi.4c00117

Jaume Ramon Otaegui, Deborah Sultan, Gyu Seong Heo, Yongjian Liu

{"title":"Positron Emission Tomography Imaging of the Adaptive Immune System in Cardiovascular Diseases.","authors":"Jaume Ramon Otaegui, Deborah Sultan, Gyu Seong Heo, Yongjian Liu","doi":"10.1021/cbmi.4c00117","DOIUrl":"https://doi.org/10.1021/cbmi.4c00117","url":null,"abstract":"Cardiovascular diseases are the leading cause of death around the globe. In recent years, a crucial role of the immune system has been acknowledged in cardiac disease progression, opening the door for immunomodulatory therapies. To this ongoing change of paradigm, positron emission tomography (PET) imaging of the immune system has become a remarkable tool to reveal immune cell trafficking and monitor disease progression and treatment response. Currently, PET imaging of the immune system in cardiovascular disease mainly focuses on the innate immune system such as macrophages, while the immune cells of the adaptive immune system including B and T cells are less studied. This can be ascribed to the lack of radiotracers specifically binding to B and T cell biomarkers compatible with PET imaging within the cardiovascular system. In this review, we summarize current knowledge about the role of the adaptive immune system (e.g., B and T cells) in major cardiovascular diseases and introduce key biomarkers for specific targeting of these immune cells and their subpopulations. Finally, we present available radiotracers for these biomarkers and propose a pathway for developing probes or optimizing those already used in other fields (e.g., oncology) to make them compatible with the cardiovascular system.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 4","pages":"209-224"},"PeriodicalIF":0.0,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Positron Emission Tomography Imaging of the Adaptive Immune System in Cardiovascular Diseases 心血管疾病中适应性免疫系统的正电子发射断层成像

Chemical & Biomedical Imaging Pub Date : 2025-03-18 DOI: 10.1021/cbmi.4c0011710.1021/cbmi.4c00117

Jaume Ramon Otaegui, Deborah Sultan, Gyu Seong Heo and Yongjian Liu*,

{"title":"Positron Emission Tomography Imaging of the Adaptive Immune System in Cardiovascular Diseases","authors":"Jaume Ramon Otaegui, Deborah Sultan, Gyu Seong Heo and Yongjian Liu*, ","doi":"10.1021/cbmi.4c0011710.1021/cbmi.4c00117","DOIUrl":"https://doi.org/10.1021/cbmi.4c00117https://doi.org/10.1021/cbmi.4c00117","url":null,"abstract":"Cardiovascular diseases are the leading cause of death around the globe. In recent years, a crucial role of the immune system has been acknowledged in cardiac disease progression, opening the door for immunomodulatory therapies. To this ongoing change of paradigm, positron emission tomography (PET) imaging of the immune system has become a remarkable tool to reveal immune cell trafficking and monitor disease progression and treatment response. Currently, PET imaging of the immune system in cardiovascular disease mainly focuses on the innate immune system such as macrophages, while the immune cells of the adaptive immune system including B and T cells are less studied. This can be ascribed to the lack of radiotracers specifically binding to B and T cell biomarkers compatible with PET imaging within the cardiovascular system. In this review, we summarize current knowledge about the role of the adaptive immune system (e.g., B and T cells) in major cardiovascular diseases and introduce key biomarkers for specific targeting of these immune cells and their subpopulations. Finally, we present available radiotracers for these biomarkers and propose a pathway for developing probes or optimizing those already used in other fields (e.g., oncology) to make them compatible with the cardiovascular system.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 4","pages":"209–224 209–224"},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00117","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Droplet Wettability on Scanning Electrochemical Cell Microscopy Performance in Stainless Steels 液滴润湿性对不锈钢扫描电化学显微镜性能的影响

Chemical & Biomedical Imaging Pub Date : 2025-03-13 DOI: 10.1021/cbmi.4c0010110.1021/cbmi.4c00101

Sarah R. Yassine, León Zendejas Medina, Egor Katkov, Robert Lacasse and Janine Mauzeroll*,

引用次数: 0

Impact of Droplet Wettability on Scanning Electrochemical Cell Microscopy Performance in Stainless Steels. 液滴润湿性对不锈钢扫描电化学显微镜性能的影响。

Chemical & Biomedical Imaging Pub Date : 2025-03-13 eCollection Date: 2025-04-28 DOI: 10.1021/cbmi.4c00101

Sarah R Yassine, León Zendejas Medina, Egor Katkov, Robert Lacasse, Janine Mauzeroll

引用次数: 0

Real-Time Visualization of Endogenous H2O2 Production in Mammalian Spheroids by Electrochemiluminescence 利用电化学发光技术实时可视化哺乳动物球体内源性H2O2生成

Chemical & Biomedical Imaging Pub Date : 2025-03-10 DOI: 10.1021/cbmi.4c0010510.1021/cbmi.4c00105

Vanshika Gupta, Francesco Falciani, Brady R. Layman, Megan L. Hill, Stefania Rapino* and Jeffrey E. Dick*,

{"title":"Real-Time Visualization of Endogenous H2O2 Production in Mammalian Spheroids by Electrochemiluminescence","authors":"Vanshika Gupta, Francesco Falciani, Brady R. Layman, Megan L. Hill, Stefania Rapino* and Jeffrey E. Dick*, ","doi":"10.1021/cbmi.4c0010510.1021/cbmi.4c00105","DOIUrl":"https://doi.org/10.1021/cbmi.4c00105https://doi.org/10.1021/cbmi.4c00105","url":null,"abstract":"Two-dimensional cell culture may be insufficient when it comes to understanding human disease. The redox behavior of complex, three-dimensional tissue is critical to understanding disease genesis and propagation. Unfortunately, few measurement tools are available for such three-dimensional models to yield quantitative insight into how reactive oxygen species (ROS) form over time. Here, we demonstrate an imaging platform for the real-time visualization of H2O2 formation for mammalian spheroids made of noncancerous human embryonic kidney cells (HEK-293) and metastatic breast cancer cells (MCF-7 and MDA-MB-231). We take advantage of the luminol and H2O2 electrochemiluminescence reaction on a transparent tin-doped indium oxide electrode. The luminescence of this reaction as a function of [H2O2] is linear (R2 = 0.98) with a dynamic range between 0.5 μM to 0.1 mM, and limit of detection of 2.26 ± 0.58 μM. Our method allows for the observation of ROS activity in growing spheroids days in advance of current techniques without the need to sacrifice the sample postanalysis. Finally, we use our procedure to demonstrate how key ROS pathways in cancerous spheroids can be up-regulated and downregulated through the addition of common metabolic drugs, rotenone and carbonyl cyanide-p-trifluoromethoxyphenylhydrazone. Our results suggest that the Warburg Effect can be studied for single mammalian cancerous spheroids, and the use of metabolic drugs allows one to implicate specific metabolic pathways in ROS formation. We expect this diagnostic tool to have wide applications in understanding the real-time propagation of human disease in a system more closely related to human tissue.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 5","pages":"310–321 310–321"},"PeriodicalIF":0.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00105","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144133895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Molecule Fluorescence Imaging of Energy-Related Catalytic Reactions 能量相关催化反应的单分子荧光成像

Chemical & Biomedical Imaging Pub Date : 2025-03-07 DOI: 10.1021/cbmi.4c0011210.1021/cbmi.4c00112

Qingdian Yan, Xianghong Li, Jianbin Luo and Ming Zhao*,

{"title":"Single-Molecule Fluorescence Imaging of Energy-Related Catalytic Reactions","authors":"Qingdian Yan, Xianghong Li, Jianbin Luo and Ming Zhao*, ","doi":"10.1021/cbmi.4c0011210.1021/cbmi.4c00112","DOIUrl":"https://doi.org/10.1021/cbmi.4c00112https://doi.org/10.1021/cbmi.4c00112","url":null,"abstract":"The pressing challenges of the energy crisis and environmental problems necessitate the pursuit of efficient and sustainable energy conversion technologies, wherein catalytic processes play a vital role in addressing these issues. Single-molecule fluorescence microscopy (SMFM) offers a transformative approach to understanding various catalytic reactions by enabling real-time visualization of molecular adsorption, diffusion, and transformation on catalytic surfaces. The unprecedented insights into the spatial distribution of active sites, catalytic heterogeneity, and the dynamics of key intermediates result in single- or subparticle level structure–property relations, thereby offering insightful perspectives for catalyst design and mechanistic understanding of energy-related catalytic processes. In this review, we provide an overview of the recent progress in using SMFM for investigating energy-related catalytic reactions. The advancement in SMFM imaging techniques for investigating nonfluorescent chemical processes is also highlighted. Finally, we conclude the review by commenting on the current challenges and prospects in advancing SMFM in energy research. We hope that the capable SMFM imaging techniques and insights will promote the development and realistic application of various energy-related catalytic reactions, together with inspiring researchers to explore the power of SMFM in other applications.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 5","pages":"280–300 280–300"},"PeriodicalIF":0.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144133940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Self-Immobilizing Photoacoustic Probe for Ratiometric In Vivo Imaging of Cu(II) in Tumors 一种用于肿瘤中Cu（II）体内比例成像的自固定光声探针

Chemical & Biomedical Imaging Pub Date : 2025-03-03 DOI: 10.1021/cbmi.4c0011510.1021/cbmi.4c00115

Qian Sun, Hang Liu, Ying Yang, Shankun Yao, Zhipeng Liu* and Zijian Guo*,

{"title":"A Self-Immobilizing Photoacoustic Probe for Ratiometric In Vivo Imaging of Cu(II) in Tumors","authors":"Qian Sun, Hang Liu, Ying Yang, Shankun Yao, Zhipeng Liu* and Zijian Guo*, ","doi":"10.1021/cbmi.4c0011510.1021/cbmi.4c00115","DOIUrl":"https://doi.org/10.1021/cbmi.4c00115https://doi.org/10.1021/cbmi.4c00115","url":null,"abstract":"Cu(II) ions play a critical role in tumor growth and metastasis, making in vivo high-resolution imaging of Cu(II) crucial for understanding its role in tumor pathophysiology. However, designing suitable molecular probes for this purpose remains challenging. Herein, we report the development of a photoacoustic probe for specific in vivo imaging of Cu(II) in tumors. This probe utilizes β-galactoside as a targeting group and incorporates a unique self-immobilization strategy. Upon β-galactosidase-mediated cleavage, the probe generates a reactive quinone methide intermediate that covalently binds to intracellular proteins, enabling selective tumor accumulation. The probe exhibits a ratiometric photoacoustic response to Cu(II) with high selectivity over that of other biological species. In vitro and in vivo studies demonstrated the efficacy of the probe for Cu(II) imaging in tumors. This research provides valuable insights into the role of Cu(II) in tumorigenesis and may facilitate the development of diagnostic and therapeutic approaches for cancer.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 4","pages":"260–266 260–266"},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorogenic Linkage Integration for Nonfluorescent Transformations (FLINT) 非荧光转化的荧光连锁集成（FLINT）

Chemical & Biomedical Imaging Pub Date : 2025-03-03 DOI: 10.1021/cbmi.4c0011410.1021/cbmi.4c00114

Bishal Pokhrel, Fatiha Farhana, Li Zuo, Rebecca L. Stratton, Pravin Pokhrel, Mohammad Akter Hossain, Jiahao Ji, Hanbin Mao and Hao Shen*,

{"title":"Fluorogenic Linkage Integration for Nonfluorescent Transformations (FLINT)","authors":"Bishal Pokhrel, Fatiha Farhana, Li Zuo, Rebecca L. Stratton, Pravin Pokhrel, Mohammad Akter Hossain, Jiahao Ji, Hanbin Mao and Hao Shen*, ","doi":"10.1021/cbmi.4c0011410.1021/cbmi.4c00114","DOIUrl":"https://doi.org/10.1021/cbmi.4c00114https://doi.org/10.1021/cbmi.4c00114","url":null,"abstract":"Since its creation, single-molecule optical imaging has revolutionized the study of catalytic processes, yet its application largely relies on probing fluorogenic reactions. To overcome this limitation, we propose the Fluorogenic Linkage Integration for Nonfluorescent Transformation (FLINT) approach, an imaging method to resolve nonfluorogenic reactions at the single-molecule level. Using glucose oxidation as a model reaction, we coupled this nonfluorogenic reaction with a fluorogenic Amplex Red (AR) → resorufin (RF) transformation to create a cascading reaction. This integration allowed us to monitor single-turnover events and extract key kinetic parameters for glucose oxidation despite their being invisible under the optical microscope. Our ensemble measurements combining cyclic voltammetry and fluorescence spectroscopy confirmed the cascade reaction mechanism and revealed first-order kinetics for both elementary reaction steps. At the single-molecule level, turnover time analysis provided detailed information on the reaction kinetics, distinguishing the relatively fast glucose oxidation from slower AR oxidation. We further confirmed the validity of the FLINT approach by comparing the catalytic performances of 5 nm gold nanoparticles (AuNPs) against that of 18 × 52 nm gold nanorods (AuNRs) and AuNP@DNA coronazymes. Furthermore, FLINT was used to evaluate the chiral selectivity of d- and l-glucose on coronazymes, suggesting the potential application of FLINT in enantioselective reactions. The FLINT approach is a significant advancement in single-molecule imaging as it enables the study of nonfluorogenic reactions with high spatiotemporal resolution.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 4","pages":"253–259 253–259"},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0