Chemical & Biomedical Imaging最新文献

{"title":"Laser-Treated Screen-Printed Carbon Electrodes for Electrochemiluminescence imaging.","authors":"Claudio Ignazio Santo, Guillermo Conejo-Cuevas, Francesco Paolucci, Francisco Javier Del Campo, Giovanni Valenti","doi":"10.1021/cbmi.4c00070","DOIUrl":"10.1021/cbmi.4c00070","url":null,"abstract":"<p><p>Electrochemiluminescence (ECL) is nowadays a powerful technique widely used in biosensing and imaging, offering high sensitivity and specificity for detecting and mapping biomolecules. Screen-printed electrodes (SPEs) offer a versatile and cost-effective platform for ECL applications due to their ease of fabrication, disposability, and suitability for large-scale production. This research introduces a novel method for improving the ECL characteristics of screen-printed carbon electrodes (SPCEs) through the application of CO₂ laser treatment following fabrication. Using advanced ECL microscopy, we analyze three distinct carbon paste-based electrodes and show that low-energy laser exposure (ranging from 7 to 12 mJ·cm^-2) enhances the electrochemical performance of the electrodes. This enhancement results from the selective removal of surface binders and contaminants achieved by the laser treatment. We employed ECL microscopy to characterize the ECL emission using a bead-based system incorporating magnetic microbeads, like those used in commercial platforms. This approach enabled high-resolution spatial mapping of the electrode surface, offering valuable insights into its electrochemical performance. Through quantitative assessment using a photomultiplier tube (PMT), it was observed that GST electrodes could detect biomarkers with high sensitivity, achieving an approximate detection limit (LOD) of 11 antibodies per μm². These findings emphasize the potential of laser-modified GST electrodes in enabling highly sensitive electrochemiluminescent immunoassays and various biosensing applications.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 12","pages":"835-841"},"PeriodicalIF":0.0,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11672215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142904022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laser-Treated Screen-Printed Carbon Electrodes for Electrochemiluminescence imaging","authors":"Claudio Ignazio Santo,&nbsp;Guillermo Conejo-Cuevas,&nbsp;Francesco Paolucci,&nbsp;Francisco Javier Del Campo* and Giovanni Valenti*,&nbsp;","doi":"10.1021/cbmi.4c0007010.1021/cbmi.4c00070","DOIUrl":"https://doi.org/10.1021/cbmi.4c00070https://doi.org/10.1021/cbmi.4c00070","url":null,"abstract":"<p >Electrochemiluminescence (ECL) is nowadays a powerful technique widely used in biosensing and imaging, offering high sensitivity and specificity for detecting and mapping biomolecules. Screen-printed electrodes (SPEs) offer a versatile and cost-effective platform for ECL applications due to their ease of fabrication, disposability, and suitability for large-scale production. This research introduces a novel method for improving the ECL characteristics of screen-printed carbon electrodes (SPCEs) through the application of CO₂ laser treatment following fabrication. Using advanced ECL microscopy, we analyze three distinct carbon paste-based electrodes and show that low-energy laser exposure (ranging from 7 to 12 mJ·cm^–2) enhances the electrochemical performance of the electrodes. This enhancement results from the selective removal of surface binders and contaminants achieved by the laser treatment. We employed ECL microscopy to characterize the ECL emission using a bead-based system incorporating magnetic microbeads, like those used in commercial platforms. This approach enabled high-resolution spatial mapping of the electrode surface, offering valuable insights into its electrochemical performance. Through quantitative assessment using a photomultiplier tube (PMT), it was observed that GST electrodes could detect biomarkers with high sensitivity, achieving an approximate detection limit (LOD) of 11 antibodies per μm². These findings emphasize the potential of laser-modified GST electrodes in enabling highly sensitive electrochemiluminescent immunoassays and various biosensing applications.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 12","pages":"835–841 835–841"},"PeriodicalIF":0.0,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00070","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142874928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multimodal Imaging Unveils the Impact of Nanotopography on Cellular Metabolic Activities","authors":"Zhi Li,&nbsp;Einollah Sarikhani,&nbsp;Sirasit Prayotamornkul,&nbsp;Dhivya Pushpa Meganathan,&nbsp;Zeinab Jahed* and Lingyan Shi*,&nbsp;","doi":"10.1021/cbmi.4c0005110.1021/cbmi.4c00051","DOIUrl":"https://doi.org/10.1021/cbmi.4c00051https://doi.org/10.1021/cbmi.4c00051","url":null,"abstract":"<p >Nanoscale surface topography is an effective approach in modulating cell-material interactions, significantly impacting cellular and nuclear morphologies, as well as their functionality. However, the adaptive changes in cellular metabolism induced by the mechanical and geometrical microenvironment of the nanotopography remain poorly understood. In this study, we investigated the metabolic activities in cells cultured on engineered nanopillar substrates by using a label-free multimodal optical imaging platform. This multimodal imaging platform, integrating two photon fluorescence (TPF) and stimulated Raman scattering (SRS) microscopy, allowed us to directly visualize and quantify metabolic activities of cells in 3D at the subcellular scale. We discovered that the nanopillar structure significantly reduced the cell spreading area and circularity compared to flat surfaces. Nanopillar-induced mechanical cues significantly modulate cellular metabolic activities with variations in nanopillar geometry further influencing these metabolic processes. Cells cultured on nanopillars exhibited reduced oxidative stress, decreased protein and lipid synthesis, and lower lipid unsaturation in comparison to those on flat substrates. Hierarchical clustering also revealed that pitch differences in the nanopillar had a more significant impact on cell metabolic activity than diameter variations. These insights improve our understanding of how engineered nanotopographies can be used to control cellular metabolism, offering possibilities for designing advanced cell culture platforms which can modulate cell behaviors and mimic natural cellular environment and optimize cell-based applications. By leveraging the unique metabolic effects of nanopillar arrays, one can develop more effective strategies for directing the fate of cells, enhancing the performance of cell-based therapies, and creating regenerative medicine applications.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 12","pages":"825–834 825–834"},"PeriodicalIF":0.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multimodal Imaging Unveils the Impact of Nanotopography on Cellular Metabolic Activities.","authors":"Zhi Li, Einollah Sarikhani, Sirasit Prayotamornkul, Dhivya Pushpa Meganathan, Zeinab Jahed, Lingyan Shi","doi":"10.1021/cbmi.4c00051","DOIUrl":"10.1021/cbmi.4c00051","url":null,"abstract":"<p><p>Nanoscale surface topography is an effective approach in modulating cell-material interactions, significantly impacting cellular and nuclear morphologies, as well as their functionality. However, the adaptive changes in cellular metabolism induced by the mechanical and geometrical microenvironment of the nanotopography remain poorly understood. In this study, we investigated the metabolic activities in cells cultured on engineered nanopillar substrates by using a label-free multimodal optical imaging platform. This multimodal imaging platform, integrating two photon fluorescence (TPF) and stimulated Raman scattering (SRS) microscopy, allowed us to directly visualize and quantify metabolic activities of cells in 3D at the subcellular scale. We discovered that the nanopillar structure significantly reduced the cell spreading area and circularity compared to flat surfaces. Nanopillar-induced mechanical cues significantly modulate cellular metabolic activities with variations in nanopillar geometry further influencing these metabolic processes. Cells cultured on nanopillars exhibited reduced oxidative stress, decreased protein and lipid synthesis, and lower lipid unsaturation in comparison to those on flat substrates. Hierarchical clustering also revealed that pitch differences in the nanopillar had a more significant impact on cell metabolic activity than diameter variations. These insights improve our understanding of how engineered nanotopographies can be used to control cellular metabolism, offering possibilities for designing advanced cell culture platforms which can modulate cell behaviors and mimic natural cellular environment and optimize cell-based applications. By leveraging the unique metabolic effects of nanopillar arrays, one can develop more effective strategies for directing the fate of cells, enhancing the performance of cell-based therapies, and creating regenerative medicine applications.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 12","pages":"825-834"},"PeriodicalIF":0.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11672213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142904024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Evolution of Sub-diffraction Chemical Imaging from Nanoscale to AI","authors":"Ji-Xin Cheng*,&nbsp;Tai-Yen Chen* and Peng Chen*,&nbsp;","doi":"10.1021/cbmi.4c0007910.1021/cbmi.4c00079","DOIUrl":"https://doi.org/10.1021/cbmi.4c00079https://doi.org/10.1021/cbmi.4c00079","url":null,"abstract":"","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 11","pages":"731–732 731–732"},"PeriodicalIF":0.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00079","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142694592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Evolution of Sub-diffraction Chemical Imaging from Nanoscale to AI.","authors":"Ji-Xin Cheng, Tai-Yen Chen, Peng Chen","doi":"10.1021/cbmi.4c00079","DOIUrl":"https://doi.org/10.1021/cbmi.4c00079","url":null,"abstract":"","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 11","pages":"731-732"},"PeriodicalIF":0.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11600144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142752184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sequencing and Optical Genome Mapping for the Adventurous Chemist","authors":"Elizabete Ruppeka Rupeika,&nbsp;Laurens D’Huys,&nbsp;Volker Leen and Johan Hofkens*,&nbsp;","doi":"10.1021/cbmi.4c0006010.1021/cbmi.4c00060","DOIUrl":"https://doi.org/10.1021/cbmi.4c00060https://doi.org/10.1021/cbmi.4c00060","url":null,"abstract":"<p >This review provides a comprehensive overview of the chemistries and workflows of the sequencing methods that have been or are currently commercially available, providing a very brief historical introduction to each method. The main optical genome mapping approaches are introduced in the same manner, although only a subset of these are or have ever been commercially available. The review comes with a deck of slides containing all of the figures for ease of access and consultation.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 12","pages":"784–807 784–807"},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142874917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sequencing and Optical Genome Mapping for the Adventurous Chemist.","authors":"Elizabete Ruppeka Rupeika, Laurens D'Huys, Volker Leen, Johan Hofkens","doi":"10.1021/cbmi.4c00060","DOIUrl":"10.1021/cbmi.4c00060","url":null,"abstract":"<p><p>This review provides a comprehensive overview of the chemistries and workflows of the sequencing methods that have been or are currently commercially available, providing a very brief historical introduction to each method. The main optical genome mapping approaches are introduced in the same manner, although only a subset of these are or have ever been commercially available. The review comes with a deck of slides containing all of the figures for ease of access and consultation.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 12","pages":"784-807"},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11673194/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142904061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Depth Mapping of Mesoporous Silica Nanoparticles in Malignant Glioma Cells Using Scattering-Type Scanning Near-Field Optical Microscopy.","authors":"George E Greaves, Alessandra Pinna, Jonathan M Taylor, Alexandra E Porter, Chris C Phillips","doi":"10.1021/cbmi.4c00053","DOIUrl":"10.1021/cbmi.4c00053","url":null,"abstract":"<p><p>Mesoporous silica nanoparticles (MSNPs) are promising nanomedicine vehicles due to their biocompatibility and ability to carry large cargoes. It is critical in nanomedicine development to be able to map their uptake in cells, including distinguishing surface associated MSNPs from those that are embedded or internalized into cells. Conventional nanoscale imaging techniques, such as electron and fluorescence microscopies, however, generally require the use of stains and labels to image both the biological material and the nanomedicines, which can interfere with the biological processes at play. We demonstrate an alternative imaging technique for investigating the interactions between cells and nanostructures, scattering-type scanning near-field optical microscopy (s-SNOM). s-SNOM combines the chemical sensitivity of infrared spectroscopy with the nanoscale spatial resolving power of scanning probe microscopy. We use the technique to chemically map the uptake of MSNPs in whole human glioblastoma cells and show that the simultaneously acquired topographical information can provide the embedding status of the MSNPs. We focus our imaging efforts on the lamellipodia and filopodia structures at the peripheries of the cells due to their significance in cancer invasiveness.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 12","pages":"842-849"},"PeriodicalIF":0.0,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11672216/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142904020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Depth Mapping of Mesoporous Silica Nanoparticles in Malignant Glioma Cells Using Scattering-Type Scanning Near-Field Optical Microscopy","authors":"George E. Greaves*,&nbsp;Alessandra Pinna,&nbsp;Jonathan M. Taylor,&nbsp;Alexandra E. Porter and Chris C. Phillips*,&nbsp;","doi":"10.1021/cbmi.4c0005310.1021/cbmi.4c00053","DOIUrl":"https://doi.org/10.1021/cbmi.4c00053https://doi.org/10.1021/cbmi.4c00053","url":null,"abstract":"<p >Mesoporous silica nanoparticles (MSNPs) are promising nanomedicine vehicles due to their biocompatibility and ability to carry large cargoes. It is critical in nanomedicine development to be able to map their uptake in cells, including distinguishing surface associated MSNPs from those that are embedded or internalized into cells. Conventional nanoscale imaging techniques, such as electron and fluorescence microscopies, however, generally require the use of stains and labels to image both the biological material and the nanomedicines, which can interfere with the biological processes at play. We demonstrate an alternative imaging technique for investigating the interactions between cells and nanostructures, scattering-type scanning near-field optical microscopy (s-SNOM). s-SNOM combines the chemical sensitivity of infrared spectroscopy with the nanoscale spatial resolving power of scanning probe microscopy. We use the technique to chemically map the uptake of MSNPs in whole human glioblastoma cells and show that the simultaneously acquired topographical information can provide the embedding status of the MSNPs. We focus our imaging efforts on the lamellipodia and filopodia structures at the peripheries of the cells due to their significance in cancer invasiveness.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 12","pages":"842–849 842–849"},"PeriodicalIF":0.0,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00053","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142874916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}