Chemical & Biomedical Imaging最新文献

{"title":"An Activity-Based Sensing Approach to Monitor Nanomaterial-Promoted Changes in Labile Metal Pools in Living Systems.","authors":"Javier Bonet-Aleta, Aidan T Pezacki, Miku Oi, Jose L Hueso, Jesus Santamaria, Christopher J Chang","doi":"10.1021/cbmi.5c00237","DOIUrl":"https://doi.org/10.1021/cbmi.5c00237","url":null,"abstract":"<p><p>Metal-based nanoparticles are a promising class of materials for diagnosis and treatment of cancer and other diseases. However, mechanisms of action of these nanomedicines remain insufficiently understood due in large part to our limited understanding of the dynamic equilibria between solid metal nanoparticles and labile metal ions generated from these nanoparticles within complex biological milieus. Here, we apply activity-based sensing to directly identify and investigate the fate of labile copper pools with metal and oxidation state-specificity generated by anticancer copper nanomedicines. We found that treatment of cells with copper-releasing nanoparticles alter labile Cu-(I)/Cu-(II) ratios through an increase in labile Cu-(II), while overall labile copper levels decrease. Labile copper release triggers compensatory responses in two major antioxidant pathways, glutathione (GSH) and nuclear factor erythroid 2-related factor 2 (NRF2), as well as in metal homeostasis to limit copper availability via regulation of copper export (ATP7B) and copper import (CTR1) proteins. These findings establish the value of activity-based sensing as a generalizable approach for labile metal imaging to help decipher molecular mechanisms of bioactive metal nanoparticles and guide the development of more effective nanomedicine diagnostics and therapies to target metal-dependent disease vulnerabilities.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 4","pages":"669-683"},"PeriodicalIF":5.7,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13126392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147823594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Serendipitous Rise of Mid-infrared Photothermal Microscopy.","authors":"Xinyu Deng, Hyeon Jeong Lee, Delong Zhang","doi":"10.1021/cbmi.5c00250","DOIUrl":"10.1021/cbmi.5c00250","url":null,"abstract":"","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 1","pages":"1-5"},"PeriodicalIF":5.7,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12848811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146088065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design, Synthesis, and Evaluation of OncoFAP Derivatives with the Albumin Binder as Novel Antitumor Radiopharmaceuticals.","authors":"Biao Yang, Yinfei Chen, Yingzhou Liu, Changyu Shan, Tianyi Zhou, Wenzhu Hu, Yuan Feng, Zhaoguo Lin, Dexing Zeng, Rui An, Xiaoli Lan, Yongkang Gai","doi":"10.1021/cbmi.5c00118","DOIUrl":"https://doi.org/10.1021/cbmi.5c00118","url":null,"abstract":"<p><p>Fibroblast activation protein (FAP), a type II transmembrane serine protease, is selectively and highly expressed on the membranes of cancer-associated fibroblasts within the stroma of various epithelial-derived malignancies. In recent years, numerous quinoline-based small organic radioligands targeting FAP, such as OncoFAP, have been developed and clinically utilized for the diagnosis of both primary and metastatic tumors. However, despite their selective accumulation, conventional FAP ligands often suffer from a relatively short half-life in tumors, limiting their efficacy in radionuclide therapy. In this study, we leveraged the structure of UAMC-1110, which was known for its high affinity for FAP, and modified it by adding functional chemical groups (unnatural amino acid, linkers, and albumin binders) to improve its pharmacokinetic and pharmacodynamic properties, thereby enhancing tumor targeting and prolonging tumor retention. Among the synthesized derivatives, XH03-11 emerged as a standout candidate due to its excellent in vitro characteristics, superior in vivo biodistribution profile, and demonstrated therapeutic efficacy in tumor-bearing mice.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 4","pages":"550-561"},"PeriodicalIF":5.7,"publicationDate":"2025-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13126357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147822971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Perspective on Understanding Transient Stimulated Raman Scattering Spectroscopy with Ramsey Interferometry.","authors":"Yue Huang, Yiran Li, Na Li, Ping Wang","doi":"10.1021/cbmi.5c00149","DOIUrl":"10.1021/cbmi.5c00149","url":null,"abstract":"<p><p>Transient stimulated Raman scattering (T-SRS), as an emerging time-domain coherent Raman scattering (TD-CRS) technique, possesses unique natural line-width-limit spectral resolution and sub-mM sensitivity, and offers a powerful spectral platform for chemical identification and imaging of biomarkers in biological tissues. However, readers may face difficulties in understanding clear physical pictures of manipulating quantum states of biomolecules by deriving wave packet interference. Here, we reinterpreted T-SRS as Ramsey interferometry driven by two femtosecond half-π operations of the superposition of biomolecules at room-temperature, an analogue to second-scale Ramsey interference of cold atoms at a temperature of ∼1 μK. This perspective contrasts the features of coherent quantum control of Ramsey interference performed in cold atomic and macroscopic biological systems. Both the theoretical reasoning and numeric simulations of quantum evolution are discussed step by step. The interdisciplinary knowledge will foster the advancement of coherent Raman spectroscopy and precision measurements in chemistry and broad biomedical applications.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 12","pages":"787-791"},"PeriodicalIF":5.7,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12728749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145835274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptide-Based Probe Targeting Angiotensin Type‑1 Receptor Identifies Pancreatic Ductal Adenocarcinoma Using Multispectral Optoacoustic Tomography.","authors":"Rohit Singh, Happy Agarwal, Ryan C Bynum, William E Grizzle, Ajay Jain, Barish H Edil, Susanna Ulahannan, Min Li, Lacey R McNally","doi":"10.1021/cbmi.5c00128","DOIUrl":"10.1021/cbmi.5c00128","url":null,"abstract":"<p><p><b>Purpose</b> Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal and aggressive malignancies with limited treatment options. Curative surgical resection of the primary pancreatic mass is frequently limited by residual microscopic tumor foci, leading to a high recurrence rate in patients. To improve surgical outcomes, visualizing remaining tumor foci with fluorescent probes is crucial to achieve complete (R0) resection. <b>Methods</b> A peptide-based imaging probe targeting the angiotensin type-1 receptor was engineered with either 555 or 750 nm reporter dyes. Immunofluorescence and NIR imaging analyses were performed to assess receptor interactions and binding probes in AT1R-positive PDAC cells and low (HT-29) control cells. Probe uptake in the complex PDAC microenvironment of orthotopic mouse models (S2VP10 and S2O13) was evaluated using multispectral optoacoustic tomography (MSOT). Ex vivo biodistribution was confirmed, and toxicity was measured in the treated mice. <b>Results</b> PDAC cells demonstrated membranous expression of angiotensin type-1 receptor, which was absent in the HT-29 control cells. An engineered NIR probe, Angio1-750, was synthesized and characterized. Docking analysis of the engineered peptide with the targeted receptor demonstrated binding. PDAC cells treated with Angio1-750 probe showed a higher signal than the HT-29 cells. The PDAC tumor had a detectable signal in MSOT (S2VP10 61 ± 7.64 a.u. at 3 h and 43.18 ± 4.51 a.u. at 24 h, <i>P</i> < 0.0004; S2O13 25.30 ± 1.53 a.u. at 3 h and 17.33 ± 0.58 a.u. at 24 h, <i>P</i> = 0.05). Biodistribution analysis revealed tumor uptake and confirmed the renal excretion of the probe. <b>Conclusions</b> The data suggest successful tumor uptake of the Angio1-750 probe and detection using MSOT in PDAC xenografts.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 3","pages":"410-421"},"PeriodicalIF":5.7,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13014318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147522588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Proceedings of Advanced Electrochemiluminescence Imaging Technology and Applications in Accurate Analysis.","authors":"Tehseen Sehar, Guichuan Wei, Kainat, Zulqurnain, Iqra, Rafay Muhammad, Fanfan Guo, Ling Zhang","doi":"10.1021/cbmi.5c00105","DOIUrl":"https://doi.org/10.1021/cbmi.5c00105","url":null,"abstract":"<p><p>Electrochemiluminescence (ECL) imaging has emerged as a highly sensitive and spatially resolved bioanalytical technique that bridges electrochemical control and optical microscopy. With its unique surface-confined emission, minimal background noise, and tunable activation, ECL imaging offers distinct advantages over fluorescence and chemiluminescence, especially for cellular and single-particle analysis. In this review, advanced strategies and wireless, multiplexed, and high-throughput bioimaging platforms, such as bipolar electrochemistry, single-electrode electrochemical system, and multielectrode array are summarized. Applications of ECL imaging methods in the single-cell analysis, nanoparticle electrocatalysis studies, intracellular and <i>in vivo</i> detection, and high-resolution bioassays are described in detail. This review has comprehensively outlined recent progress in these advanced technologies and presenting some emerging tools such as miniaturized devices, wireless energy transmission, and next-generation materials driving the transformation of ECL imaging into a robust platform for diagnostics and research. This work has not only summarized past progress but also explored future breakthroughs in precision diagnostics and electrochemical microscopy.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 4","pages":"485-509"},"PeriodicalIF":5.7,"publicationDate":"2025-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13126399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147823350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel \"C-Clamp\" Design of Highly Selective Small-Molecule Sensors for Kinase Proteins.","authors":"Shaista Ahmad, Aleksandr A Barashkin, Haniyeh Jadidi, Alan Calderon, Harouna Amadou, Adam B Ornberg, Evgueni E Nesterov, Irina V Nesterova","doi":"10.1021/cbmi.5c00185","DOIUrl":"https://doi.org/10.1021/cbmi.5c00185","url":null,"abstract":"<p><p>We report an innovative design concept for small-molecule fluorescent sensors that are highly selective for protein targets. The sensor's enhanced selectivity stems from a \"C-clamping\" paradigm where three different binding domains cooperatively interact with a protein target. We established the sensor's performance both <i>in vitro</i> and in live cells using epidermal growth factor receptor (EGFR) tyrosine kinase as a proof-of-concept target. Detailed combined quantum mechanics/molecular mechanics computational studies strongly support both the experimentally established specificity and the \"C-clamp\" binding mechanism. To demonstrate the sensor's practical utility, we developed a single-compound assay for the quantitative profiling of EGFR kinase inhibitors upon fluorescent imaging in live cells. When bound to the protein target, the sensor is emissive in the near-infrared region and yields a turn-on quantitative fluorescent response toward small-molecule tyrosine kinase inhibitors. Furthermore, this sensing system produces differentiated responses to a series of clinically relevant EGFR kinase inhibitors in native environments. Overall, we envision that this work will empower the development of small-molecule systems for highly specific protein recognition and sensing, become an invaluable tool for assessing small-molecule/protein engagement, and be extended toward live-cell screening and fluorescent imaging of other important biomolecular targets.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 4","pages":"658-668"},"PeriodicalIF":5.7,"publicationDate":"2025-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13126394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147823398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solvatochromic BODIPYs with Polarity-Independent Absorption for Functional Super-resolution Imaging of Live Cell Membranes.","authors":"Yunshu Liu, Xiayi Gong, Bryn Yoder, Jessica M Gluck, Nelson R Vinueza, Xiaojing Liu, Yang Zhang","doi":"10.1021/cbmi.5c00131","DOIUrl":"https://doi.org/10.1021/cbmi.5c00131","url":null,"abstract":"<p><p>Functional super-resolution microscopy (SRM) has recently emerged as a powerful technique for concurrently mapping nanoscale physicochemical properties of the subcellular environment. This is accomplished by capturing both the spatial localization and single-molecule fluorescence spectral shifts of environmentally sensitive fluorescent probes. However, current fluorophore choices for functional SRM are largely limited to Nile Red derivatives, which suffer from several limitations. Here we report two solvatochromic and far-red-emitting fluorophores with polarity-independent absorption specifically tailored for functional SRM, based on a boron dipyrromethene (BODIPY) chromophore. We systematically characterize their photophysical and solvatochromic properties and directly compare their performance to that of Nile Red. A zwitterionic lipid is introduced to the dye to enable transient-binding-based single-molecule switching and to achieve selective targeting of the plasma membrane in live cells. The functional SRM capability of this probe is demonstrated by mapping nanoscale membrane polarity changes under the influence of cholesterol in live U2OS cells with a localization precision of 16-20 nm.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 4","pages":"527-532"},"PeriodicalIF":5.7,"publicationDate":"2025-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13126358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147823343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Folate Receptor Alpha Targeted Immuno-Positron Emission Tomography Probe for Noninvasive Imaging in Triple-Negative Breast Cancer.","authors":"Xiuli Ma, Nan Yang, Xiaoyi Guo, Jiayue Liu, Yan Zhang, Hua Zhu, Yiqiang Liu, Zhi Yang","doi":"10.1021/cbmi.5c00133","DOIUrl":"https://doi.org/10.1021/cbmi.5c00133","url":null,"abstract":"<p><p>Triple-negative breast cancer (TNBC) is characterized by considerable heterogeneity and a poor response to chemotherapy. While folate receptor alpha (FRα) has attracted growing interest as a therapeutic target due to its frequent overexpression in numerous epithelial-derived tumors, we sought to visually evaluate its specific expression profile in TNBC. Therefore, we first evaluated FRα expression in 176 clinical TNBC specimens. The vast majority (84%) of samples showed no FRα expression, with the remainder showing low to moderate positivity (1+ to 3+), and no significant correlation was found with standard clinicopathological features. Given the low and variable FRα expression in TNBC, we developed an immunoPET probe, ⁸⁹Zr-DFO-Mirvetuximab, for noninvasive quantification. This probe was synthesized by conjugating the anti-FRα antibody Mirvetuximab with the chelator desferrioxamine (DFO), followed by ⁸⁹Zr-labeling, exhibiting high radiochemical purity (>95%) and exceptional stability. Cell uptake assays revealed time-dependent accumulation, which was blocked by excess Mirvetuximab, demonstrating FRα-specific targeting. Binding affinity measurement confirmed high affinity for FRα, with a dissociation constant (<i>K</i> _d) of 27.46 nM. We further validated the probe in vivo using micro-PET/CT imaging and biodistribution studies in mice bearing CAL51 (FRα-high) and MDA-MB-231 (FRα-low) tumors. The results demonstrated significantly higher uptake in CAL51 models compared to MDA-MB-231 models (17.17 ± 2.24%ID/g vs 3.79 ± 1.15%ID/g, <i>P</i> < 0.001), which was further confirmed by subsequent immunohistochemical analysis. In conclusion, ⁸⁹Zr-DFO-Mirvetuximab holds significant potential for noninvasively identifying TNBC patients with sufficient FRα expression for targeted therapies like Mirvetuximab soravtansine, thereby supporting improved patient stratification and treatment response monitoring.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 4","pages":"562-570"},"PeriodicalIF":5.7,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13126377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147823382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Donor Modulation of Strong Near-Infrared Lipid-Droplet Probes for in Vivo Diagnosis of Nonalcoholic Fatty Liver Disease.","authors":"Caihong Xiang, Xiang Wang, Ruli Zheng, Xupeng Liu, Xiaowan Han, Zhisheng Gui, Qingping Xiong, Lianrui Hu, Guangle Niu","doi":"10.1021/cbmi.5c00167","DOIUrl":"https://doi.org/10.1021/cbmi.5c00167","url":null,"abstract":"<p><p>Lipid droplet accumulation is a pathological hallmark of nonalcoholic fatty liver disease (NAFLD); however, the utility of current lipid droplet-targeted fluorescent probes for in vivo diagnosis is hindered by shallow tissue penetration and strong background autofluorescence. To overcome these challenges, here, we report three donor-acceptor-acceptor solvatochromic probes (CTBT-ANPy, PTBT-ANPy, and MOPTBT-ANPy) with tunable electron-donating groups, enabling near-infrared (NIR) imaging of lipid droplets and in vivo NAFLD diagnosis. Owing to multiple intramolecular interactions and restriction of twisted intramolecular charge transfer, these probes exhibit intense NIR emissions (up to 709 nm, quantum yield of 34.2%) in low-polarity media. These lipophilic probes display high lipid-droplet specificity with bright light-up signals and wash-free capacity. With the aid of NIR-emissive MOPTBT-ANPy, in vivo high-contrast fluorescent discrimination of NAFLD in a mouse model was successfully achieved. This study underscores the potential of high-performance NIR probes for noninvasive accurate NAFLD diagnosis and monitoring.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 4","pages":"638-647"},"PeriodicalIF":5.7,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13126355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147823261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}