Chemical & Biomedical Imaging最新文献

{"title":"Tetrahedral DNA Framework–Bridged Aptasensing Method for Ultrasensitive Determination of Cardiac Troponin I","authors":"Jiaqi Cai,&nbsp;Wenshuai Zhou,&nbsp;Liang Gao,&nbsp;Xiaolin Yang,&nbsp;Meng Li,&nbsp;Chengxiao Zhang,&nbsp;Honglan Qi","doi":"10.1021/cbmi.5c00292","DOIUrl":"https://doi.org/10.1021/cbmi.5c00292","url":null,"abstract":"<p>The development of a sensitive and accurate method forthe determinationof cardiac troponin I (cTnI), as an ideal biomarker of acute myocardialinfarction (AMI), is critical for the early diagnosis of AMI. In thiswork, a tetrahedral DNA framework (TDF)-bridged aptasensing methodwas proposed for the ultrasensitive determination of cTnI via electrogeneratedchemiluminescence (ECL) and fluorescence imaging methods, in whicha specific anti-cTnI aptamer-tagged tetrahedral DNA framework (TDF-aptamer)was designed as a capture probe by incorporating a specific aptameragainst cTnI within the three-dimensional TDF. The TDF-aptamer wasmodified onto a gold electrode surface through the Au–S bond,providing a specifically captured platform for cTnI with a 3D bindingspace at the nanoscale. One microsized signal probe (Ab2-biotin•SA@MB-Ru1)was synthesized by using magnetic Fe₃O₄ as carriersto load a large amount of ruthenium complex and a specific cTnI antibody.After the sandwich assay among the modified TDF-aptamer, cTnI, andthe signal probe, both ECL and fluorescence signals were recordedfor the cTnI assay with desirable selectivity and high sensitivity(linear range of 5 to 80 pg/mL using ECL intensity detection and 0.5to 500 pg/mL using ECL and fluorescence imaging methods). This workdemonstrated that the combination of the specific TDF-aptamer andmicrosized Ab2-biotin•SA@MB-Ru1 within the imaging method canenhance the specific recognition and detection sensitivity for cTnI,which is promising for AMI diagnosis.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 9","pages":"2065–2073"},"PeriodicalIF":6.7,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149067493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imaging from Macro to Nanoscale: Multimodal Advances in Chemical and Biomedical Imaging","authors":"Rong Zhou,&nbsp;Peng Chen,&nbsp;Neso Sojic","doi":"10.1021/cbmi.6c00066","DOIUrl":"https://doi.org/10.1021/cbmi.6c00066","url":null,"abstract":"<p>Imaging increasingly serves as a multiscale frameworkfor linkingmolecular mechanisms to cellular behavior, tissue architecture, andorgan phenotypes in biology and unraveling fundamental processes inchemistry, physics and materials science. This Perspective highlightsrecent advances in chemical and biomedical imaging across macro-,micro-, and nanoscales, using representative examples published in <em>Chemical and Biomedical Imaging</em> (CBMI). At the macroscale,we discuss chemically selective MRI, including endogenous and exogenousCEST strategies, together with photoacoustic imaging as a hybrid modalitywith functional and chemical contrast. At the microscale, we considerfluorescence, label-free optical and vibrational imaging, and selectedX-ray approaches that expand sensitivity, specificity, and temporalresolution in biological and materials systems. At the nanoscale,we highlight super-resolution fluorescence microscopy, single-moleculemethods, tip-enhanced Raman spectroscopy, and correlative imagingstrategies that resolve local heterogeneity and molecular organization.Across scales, a common theme emerges that advances in probes, contrastmechanisms, instrumentation, and sample handling are enabling chemicallyinformed imaging that connects molecular specificity with biologicalcontext.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 9","pages":"1876–1885"},"PeriodicalIF":6.7,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149071050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Imaging of In Vivo Zinc Distribution Using Nuclear and Magnetic Resonance Imaging","authors":"Changhua Mu,&nbsp;Xiaoxi Liu,&nbsp;Umama Ali,&nbsp;Peder E. Z. Larson,&nbsp;Sunita P. Ho,&nbsp;Robert R. Flavell","doi":"10.1021/cbmi.5c00264","DOIUrl":"https://doi.org/10.1021/cbmi.5c00264","url":null,"abstract":"<p>Zinc, a ubiquitous trace element, is essential to numerousbiologicalprocesses, including enzymatic catalysis, gene expression, and cellularsignaling. Precise zinc homeostasis, maintained by a complex interplayof transporters, storage proteins, and regulatory mechanisms, is criticalfor cellular function and organismal health. Disrupted zinc homeostasishas been implicated in the pathophysiology of diabetes, neurodegenerativedisorders, and cancers, through alterations in zinc-dependent signalingpathways, oxidative stress responses, and apoptosis mechanisms. Thus,zinc biodistribution is a potential biomarker enabling improved detectionof zinc-related pathologies, assessment of disease prognosis, anddevelopment of more targeted prevention strategies. While fluorescencetechniques have advanced our understanding of cellular zinc, theirapplication in human studies remains restricted. This highlights theneed for zinc-specific biosensors that can be integrated with clinicalimaging technologies, such as Positron Emission Tomography (PET),Single Photon Emission Computed Tomography (SPECT), and Magnetic ResonanceImaging (MRI), to enable in vivo visualization of zinc distributionand dynamics. This review describes the mechanistic understandingof zinc-associated diseases, details the design principles of molecularzinc biosensors (including radiopharmaceuticals and MRI contrast agents),and identifies the challenges and future directions for developingmore specific and sensitive probes. For nuclear imaging (PET and SPECT),this includes probes utilizing radioactive zinc ions ⁶⁵Zn, ⁶²Zn, and ⁶³Zn, and zinc chelators labeledwith isotopes like ¹⁸F, ¹¹C, and ¹²⁵I. For MRI, detection mechanisms leverage relaxivity (<em>r</em>₁), Chemical Exchange Saturation Transfer (CEST), andhyperpolarization technologies. These imaging modalities exhibit thepotential of bridging the gap between cellular and systemic zinc organ-leveldynamics, offering a “horizontal” perspective on diseasepathogenesis and facilitating precision medicine.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 9","pages":"1886–1914"},"PeriodicalIF":6.7,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149067462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid Metabolism-Related Rapid Pathological Diagnosis and Staging of Breast Tissue Sections Using Two-Photon Fluorescence Imaging Assisted with a Carbonized Polymer Dot Probe","authors":"Zitong Yu,&nbsp;Jiaqi Wang,&nbsp;Ying Liu,&nbsp;Weiqing Xu,&nbsp;Bing Han,&nbsp;Shuping Xu","doi":"10.1021/cbmi.6c00025","DOIUrl":"https://doi.org/10.1021/cbmi.6c00025","url":null,"abstract":"<p>Rapid and accurate intraoperative pathologic diagnosisis essentialfor intraoperative decision-making, improving patient prognosis, andavoiding reoperation. This study developed a unique rapid pathologicaldiagnosis and staging technique for intraoperative tissue sectionsbased on lipidomic relevance, using two-photon fluorescence (TPF)imaging assisted by a lipid-targeted carbonized polymer dot (CPD)probe (∼8 nm) and multiple classification algorithms, includingmachine learning and deep convolutional networks. This platform enablesultrabright TPF imaging in response to lipid microenvironments andcan be completed within 10 min. Its feasibility was validated throughrapid diagnostics of intraoperative frozen sections of breast tissues.Due to abnormal levels of lipid metabolism, normal breast tissuesand pathologically staged I–III cancerous tissues were welldistinguishable, and the sensitivities and specificities for pathologicdiagnosis were all above 90%. The combination of CPD and TPF microscopyprovides a rapid and accurate method for detecting lipidomes in pathologicalfrozen sections, marking a significant advancement over hematoxylinand eosin (H&amp;E)-staining-based pathological diagnosis in termsof speed, ease of operation, and relevance to chemical components.This study is vital for advancing lipidome-related research and enhancingthe understanding of the lipidomic atlas.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 9","pages":"2091–2101"},"PeriodicalIF":6.7,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149067491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SpotOn: A Tool to Enhance Echo Acoustic Standard Deposition for Quantitative Mass Spectrometry Imaging","authors":"Emily R. Sekera,&nbsp;Nathaniel Twarog,&nbsp;Rebekah DeVries,&nbsp;P. Jake Slavish,&nbsp;John J. Bowling,&nbsp;Anang A. Shelat","doi":"10.1021/cbmi.6c00010","DOIUrl":"https://doi.org/10.1021/cbmi.6c00010","url":null,"abstract":"<p>The quantitative evaluation of therapeutics in the drugdiscoverypipeline is essential for elucidating their pharmacokinetic and pharmacodynamicprofiles within complex biological systems. Traditionally, autoradiographyand liquid chromatography-mass spectrometry (LC-MS) have been employedfor such analyses. However, matrix-assisted laser desorption ionizationmass spectrometry imaging (MALDI-MSI) is emerging as a promising alternative,offering a more rapid untargeted workflow. Quantitative MALDI-MSI(qMSI) relies on accurate calibration to achieve robust and reproduciblequantification of analytes. Here, we present an open-source softwaretool, SpotOn, designed to facilitate the discrete deposition of low-volumecalibration standards onto glass slides or tissue sections. By enablingprecise placement and significantly reducing the volume of standardsrequired, our tool affords the generation of more calibration pointsacross a wider dynamic range, thereby improving the robustness andaccuracy of quantitative analyses. Software was validated using JQ1on both glass slides and murine liver tissue. The resulting calibrationcurves demonstrated strong linearity, with coefficients of determination(<em>R</em>²) exceeding 0.92. This tool streamlinesthe qMSI workflow, conserves valuable reagents, and enhances analyticalreliability, making it a helpful resource.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 9","pages":"2119–2125"},"PeriodicalIF":6.7,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149067567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expanding the Medicinal Chemistry Toolbox for PET Imaging: Aliphatic 11C/18F-Trifluoromethylation as a Late-Stage Strategy","authors":"Xin Zhou,&nbsp;Yinlong Li,&nbsp;Steven H. Liang","doi":"10.1021/cbmi.6c00096","DOIUrl":"https://doi.org/10.1021/cbmi.6c00096","url":null,"abstract":"","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 9","pages":"1872–1875"},"PeriodicalIF":6.7,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149067463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rare-Earth Nanomaterials for Advanced Theranostics of Atherosclerosis","authors":"Qianqian Bai,&nbsp;Wenlong Ma,&nbsp;Feiyu Zhao,&nbsp;Abdulrahman AL-Ammari,&nbsp;Shanyue Guan,&nbsp;Byung Ho Kang,&nbsp;Xiao Yu Tian,&nbsp;Lei Zhang","doi":"10.1021/cbmi.5c00284","DOIUrl":"https://doi.org/10.1021/cbmi.5c00284","url":null,"abstract":"<p>Atherosclerosis (AS) poses major challenges for precisediagnosisand targeted therapy due to its complex inflammatory and oxidativeplaque microenvironment. This review begins by dissecting the cellularand molecular hallmarks of AS progression, from endothelial dysfunctionto plaque rupture, identifying key targets including adhesion molecules,scavenger receptors, and immune costimulatory pathways that can beexploited for nanotheranostic intervention. Rare-earth nanoparticles(RENPs) offer a versatile platform that integrates multimodal imagingwith therapeutic functions. We highlight the strategic design of RENPs,including core–shell engineering, surface functionalization,and doping strategies, to enhance NIR-II luminescence, lifetime multiplexing,and X-ray-activated persistent luminescence for high-resolution vascularimaging and fluorescence-guided surgery. Their intrinsic enzyme-mimeticactivities, particularly the cerium-based nanozymes that scavengereactive oxidative species, are examined as a key therapeutic mechanismtargeting oxidative stress within plaques. A critical discussion ofbiocompatibility analyzes the element- and form-dependent toxicityprofiles, emphasizing the divergent effects between therapeutic nanomaterialsand potentially harmful rare-earth ions. These insights underscorethe necessity of rigorous safety evaluations alongside functionaldevelopment. Finally, we outline future directions and challengesin the clinical translation of RENP-based theranostic strategies,emphasizing the need for optimized targeting, biosafety, and multifunctionalintegration to achieve the precise diagnosis and effective treatmentof atherosclerotic cardiovascular diseases.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 9","pages":"1933–1961"},"PeriodicalIF":6.7,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149071051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Holographic Fingerprinting Reveals Oligomer-Driven Phase Separation in Bovine Serum Albumin","authors":"Fook Chiong Cheong,&nbsp;Seung Y. Lee,&nbsp;Sujata Bais,&nbsp;Satyam Khanal,&nbsp;Saumya Saurabh","doi":"10.1021/cbmi.5c00258","DOIUrl":"https://doi.org/10.1021/cbmi.5c00258","url":null,"abstract":"<p>Biomolecular condensates formed throughphase separationare fundamentalto the cellular organization. Although the physical principles underlyingthe phase separation of intrinsically disordered proteins are wellunderstood, the molecular determinants of condensate formation inglobular proteins remain elusive. Here, we employ holographic particlecharacterization, a label-free, high-throughput imaging technique,to investigate the self-assembly of bovine serum albumin (BSA), amodel globular protein. We show that this technique reliably differentiatesbetween liquid-like and gel-like condensates by their distinct refractiveindices and morphologies. Coupled with size-exclusion chromatography,our analysis reveals that BSA phase separation strictly depends onhigher-order oligomeric assemblies. Monomeric and dimeric fractionsfailed to form condensates under identical crowding conditions. Furthermore,the internal packing density of these condensates is tunable via pH-drivenprotonation changes but remains insensitive to ionic screening. Thesefindings support a model of “emergent multivalency,”in which oligomerization increases the effective interaction valenceand creates a structural scaffold that enables hydrophobically stabilizedphase separation, consistent with phase separation coupled to percolationframeworks.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 9","pages":"2023–2031"},"PeriodicalIF":6.7,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149067459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AI for Chemical and Biomedical Imaging: From Data Acquisition to Actionable Insight","authors":"Yong Fan,&nbsp;Sokratis Makrogiannis,&nbsp;Rong Zhou,&nbsp;Peng Chen","doi":"10.1021/cbmi.6c00198","DOIUrl":"https://doi.org/10.1021/cbmi.6c00198","url":null,"abstract":"","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 9","pages":"1869–1871"},"PeriodicalIF":6.7,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149067578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemiluminescence and Photoinduced Electrochemiluminescence Probing of Energy Conversion Catalysis","authors":"Jing-Yi Chen,&nbsp;Cong-Hui Xu,&nbsp;Wei Zhao","doi":"10.1021/cbmi.6c00028","DOIUrl":"https://doi.org/10.1021/cbmi.6c00028","url":null,"abstract":"<p>Electrochemiluminescence (ECL) and its photoinduced variant,photoinducedelectrochemiluminescence (PECL), have progressed beyond analyticaldetection tools to emerge as powerful spatiotemporal probes for interfacialelectrocatalysis and photoelectrochemical processes. By transducinglocal electron-transfer events, reactive intermediates, and charge-carrierdynamics into photon emission, ECL and PECL provide a distinctiveelectrochemical–optical platform that combines high sensitivity,negligible optical background, and precise spatiotemporal controllability.This review systematically surveys recent methodological advancesand representative applications of ECL and PECL in energy conversioncatalysis. ECL-based approaches are shown to enable visualizationof catalytic heterogeneity, dynamic evolution, and structure–activityrelationships across a wide range of catalytic materials. Particularemphasis is placed on mechanistic insights into key electrocatalyticreactions, including the oxygen reduction, oxygen evolution, and hydrogenevolution reactions, in which spatial and temporal resolved ECL signalselucidate reaction pathways, intermediate dynamics, and local microenvironmentalvariations. In parallel, PECL is discussed as a selective probe forphotoinduced charge-carrier utilization, recombination, and interfacialkinetics in photocatalytic and photoelectrochemical systems. Finally,current challenges and future perspectives for advancing ECL- andPECL-based methodologies in energy-related catalysis are outlined.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 9","pages":"1962–1977"},"PeriodicalIF":6.7,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149067470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}