Chemical & Biomedical Imaging最新文献_第2页

Monitoring Macrophage Polarization with Gene Expression Reporters and Bioluminescence Phasor Analysis.

Chemical & Biomedical Imaging Pub Date : 2024-10-03 eCollection Date: 2024-11-25 DOI: 10.1021/cbmi.4c00049

Giulia Tedeschi, Mariana X Navarro, Lorenzo Scipioni, Tanvi K Sondhi, Jennifer A Prescher, Michelle A Digman

{"title":"Monitoring Macrophage Polarization with Gene Expression Reporters and Bioluminescence Phasor Analysis.","authors":"Giulia Tedeschi, Mariana X Navarro, Lorenzo Scipioni, Tanvi K Sondhi, Jennifer A Prescher, Michelle A Digman","doi":"10.1021/cbmi.4c00049","DOIUrl":"10.1021/cbmi.4c00049","url":null,"abstract":"Macrophages exhibit a spectrum of behaviors upon activation and are generally classified as one of two types: inflammatory (M1) or anti-inflammatory (M2). Tracking these phenotypes in living cells can provide insight into immune function but remains a challenging pursuit. Existing methods are mostly limited to static readouts or are difficult to employ for multiplexed imaging in complex 3D environments while maintaining cellular resolution. We aimed to fill this void using bioluminescent technologies. Here we report genetically engineered luciferase reporters for the long-term monitoring of macrophage polarization via spectral phasor analysis. M1- and M2-specific promoters were used to drive the expression of bioluminescent enzymes in macrophage cell lines. The readouts were multiplexed and discernible in both 2D and 3D formats with single-cell resolution in living samples. Collectively, this work expands the toolbox of methods for monitoring macrophage polarization and provides a blueprint for monitoring other multifaceted networks in heterogeneous environments.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 11","pages":"765-774"},"PeriodicalIF":0.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11600157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142752150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring Macrophage Polarization with Gene Expression Reporters and Bioluminescence Phasor Analysis 利用基因表达报告和生物发光相位分析监测巨噬细胞极化

Chemical & Biomedical Imaging Pub Date : 2024-10-03 DOI: 10.1021/cbmi.4c0004910.1021/cbmi.4c00049

Giulia Tedeschi, Mariana X. Navarro, Lorenzo Scipioni, Tanvi K. Sondhi, Jennifer A. Prescher* and Michelle A. Digman*,

{"title":"Monitoring Macrophage Polarization with Gene Expression Reporters and Bioluminescence Phasor Analysis","authors":"Giulia Tedeschi, Mariana X. Navarro, Lorenzo Scipioni, Tanvi K. Sondhi, Jennifer A. Prescher* and Michelle A. Digman*, ","doi":"10.1021/cbmi.4c0004910.1021/cbmi.4c00049","DOIUrl":"https://doi.org/10.1021/cbmi.4c00049https://doi.org/10.1021/cbmi.4c00049","url":null,"abstract":"Macrophages exhibit a spectrum of behaviors upon activation and are generally classified as one of two types: inflammatory (M1) or anti-inflammatory (M2). Tracking these phenotypes in living cells can provide insight into immune function but remains a challenging pursuit. Existing methods are mostly limited to static readouts or are difficult to employ for multiplexed imaging in complex 3D environments while maintaining cellular resolution. We aimed to fill this void using bioluminescent technologies. Here we report genetically engineered luciferase reporters for the long-term monitoring of macrophage polarization via spectral phasor analysis. M1- and M2-specific promoters were used to drive the expression of bioluminescent enzymes in macrophage cell lines. The readouts were multiplexed and discernible in both 2D and 3D formats with single-cell resolution in living samples. Collectively, this work expands the toolbox of methods for monitoring macrophage polarization and provides a blueprint for monitoring other multifaceted networks in heterogeneous environments.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 11","pages":"765–774 765–774"},"PeriodicalIF":0.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142694514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid and Large-Scale Synthesis of Chiral and Fluorescent Sulfur Quantum Dots for Intracellular Temperature Monitoring

Chemical & Biomedical Imaging Pub Date : 2024-09-20 DOI: 10.1021/cbmi.4c0005210.1021/cbmi.4c00052

Li Zhao, Tianjian Sha, Yufu Liu, Qingsong Mei*, Haibin Li, Pinghua Sun, Haibo Zhou* and Huaihong Cai*,

{"title":"Rapid and Large-Scale Synthesis of Chiral and Fluorescent Sulfur Quantum Dots for Intracellular Temperature Monitoring","authors":"Li Zhao, Tianjian Sha, Yufu Liu, Qingsong Mei*, Haibin Li, Pinghua Sun, Haibo Zhou* and Huaihong Cai*, ","doi":"10.1021/cbmi.4c0005210.1021/cbmi.4c00052","DOIUrl":"https://doi.org/10.1021/cbmi.4c00052https://doi.org/10.1021/cbmi.4c00052","url":null,"abstract":"The large-scale preparation of fluorescent nanomaterials with laboratory-relevant chemical and optical properties will greatly forward their consumer market applications; however, it still remains challenging. In this work, a universal strategy was developed for the rapid and large-scale synthesis of fluorescent sulfur quantum dots that recently has drawn great attention because of their unique optical characteristics. From the fact that empty 3d orbitals of sulfide species are able to bind with lone-pair π electrons of the heteroatomic groups, many amino-group containing compounds, such as amino acid and polyethylenimine molecules, were exploited to synthesize sulfur quantum dots. This 10 min preparation period endowed sulfur quantum dots with bright blue fluorescence and also chirality. Due to the user-friendly and rapid operation, this strategy can be extended to the large-scale synthesis of sulfur quantum dots with a yield of 16.844 g for one batch of experiment. Moreover, it was found that the sulfur quantum dots exhibited a reversible temperature-dependent luminescent property with a sensitivity of 0.72%/°C, which showed excellent intracellular temperature monitoring capability for inflammation-related disease diagnostics.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 12","pages":"817–824 817–824"},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142874938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescent Water-Soluble Polycationic Chitosan Polymers as Markers for Biological 3D Imaging 作为生物三维成像标记的荧光水溶性聚阳离子壳聚糖聚合物

Chemical & Biomedical Imaging Pub Date : 2024-09-10 DOI: 10.1021/cbmi.4c0002810.1021/cbmi.4c00028

Srishti Vajpayee, Tiziana Picascia, Fabio Casciano, Elisabetta Viale, Luca Ronda, Stefano Bettati, Daniela Milani, Norbert Gretz and Rossana Perciaccante*,

{"title":"Fluorescent Water-Soluble Polycationic Chitosan Polymers as Markers for Biological 3D Imaging","authors":"Srishti Vajpayee, Tiziana Picascia, Fabio Casciano, Elisabetta Viale, Luca Ronda, Stefano Bettati, Daniela Milani, Norbert Gretz and Rossana Perciaccante*, ","doi":"10.1021/cbmi.4c0002810.1021/cbmi.4c00028","DOIUrl":"https://doi.org/10.1021/cbmi.4c00028https://doi.org/10.1021/cbmi.4c00028","url":null,"abstract":"Over the last decades, various tissue-clearing techniques have broken the ground for the optical imaging of whole organs and whole-organisms, providing complete representative data sets of three-dimensional biological structures. Along with advancements in this field, the development of fluorescent markers for staining vessels and capillaries has offered insights into the complexity of vascular networks and their impact on disease progression. Here we describe the use of a modified water-soluble chitosan linked to cyanine dyes in combination with ethyl cinnamate-based optical tissue clearing for the 3D visualization of tissue vasculature in depth. The water-soluble fluorescent Chitosans have proven to be an optimal candidate for labeling both vessels and capillaries ex vivo thanks to their increased water solubility, high photostability, and optical properties in the near-infrared window. In addition, the nontoxicity of these markers broadens their applicability to in vitro and in vivo biological applications. Despite the availability of other fluorescent molecules for vascular staining, the present study, for the first time, demonstrates the potential of fluorescent chitosans to depict vessels at the capillary level and opens avenues for advancing the diagnostic field by reducing the complexity and costs of the currently available procedures.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 10","pages":"721–730 721–730"},"PeriodicalIF":0.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142550365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescent Water-Soluble Polycationic Chitosan Polymers as Markers for Biological 3D Imaging. 作为生物三维成像标记的荧光水溶性多阳离子壳聚糖聚合物。

Chemical & Biomedical Imaging Pub Date : 2024-09-10 eCollection Date: 2024-10-28 DOI: 10.1021/cbmi.4c00028

Srishti Vajpayee, Tiziana Picascia, Fabio Casciano, Elisabetta Viale, Luca Ronda, Stefano Bettati, Daniela Milani, Norbert Gretz, Rossana Perciaccante

{"title":"Fluorescent Water-Soluble Polycationic Chitosan Polymers as Markers for Biological 3D Imaging.","authors":"Srishti Vajpayee, Tiziana Picascia, Fabio Casciano, Elisabetta Viale, Luca Ronda, Stefano Bettati, Daniela Milani, Norbert Gretz, Rossana Perciaccante","doi":"10.1021/cbmi.4c00028","DOIUrl":"10.1021/cbmi.4c00028","url":null,"abstract":"Over the last decades, various tissue-clearing techniques have broken the ground for the optical imaging of whole organs and whole-organisms, providing complete representative data sets of three-dimensional biological structures. Along with advancements in this field, the development of fluorescent markers for staining vessels and capillaries has offered insights into the complexity of vascular networks and their impact on disease progression. Here we describe the use of a modified water-soluble chitosan linked to cyanine dyes in combination with ethyl cinnamate-based optical tissue clearing for the 3D visualization of tissue vasculature in depth. The water-soluble fluorescent Chitosans have proven to be an optimal candidate for labeling both vessels and capillaries ex vivo thanks to their increased water solubility, high photostability, and optical properties in the near-infrared window. In addition, the nontoxicity of these markers broadens their applicability to in vitro and in vivo biological applications. Despite the availability of other fluorescent molecules for vascular staining, the present study, for the first time, demonstrates the potential of fluorescent chitosans to depict vessels at the capillary level and opens avenues for advancing the diagnostic field by reducing the complexity and costs of the currently available procedures.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 10","pages":"721-730"},"PeriodicalIF":0.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11522997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142559439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptide PET Imaging: A Review of Recent Developments and a Look at the Future of Radiometal-Labeled Peptides in Medicine. 多肽 PET 成像：多肽正电子发射计算机断层成像：最新发展综述及放射性同位素标记多肽在医学中的未来展望》（Peptide PET Imaging: A Review of Recent Developments and a Look at the Future of Radiometal-Labeled Peptides in Medicine.

Chemical & Biomedical Imaging Pub Date : 2024-08-23 eCollection Date: 2024-09-23 DOI: 10.1021/cbmi.4c00030

Majed Shabsigh, Lee A Solomon

引用次数: 0

Peptide PET Imaging: A Review of Recent Developments and a Look at the Future of Radiometal-Labeled Peptides in Medicine 多肽 PET 成像：近期发展回顾与放射性同位素标记肽在医学中的未来展望

Chemical & Biomedical Imaging Pub Date : 2024-08-22 DOI: 10.1021/cbmi.4c0003010.1021/cbmi.4c00030

Majed Shabsigh, and , Lee A. Solomon*,

引用次数: 0

DNA-FRET Constructs Enable Multiplexed Fluorescence Detection at the Single-Molecule Level DNA-FRET 构建可实现单分子水平的多重荧光检测

Chemical & Biomedical Imaging Pub Date : 2024-08-08 DOI: 10.1021/cbmi.4c0005410.1021/cbmi.4c00054

Juan Wang, and , Hanyang Yu*,

引用次数: 0

Milk Exosome-Glow Nanosystem for Cancer Cellular and Tissue Bioimaging. 用于癌症细胞和组织生物成像的牛奶外泌体-光纳米系统。

Chemical & Biomedical Imaging Pub Date : 2024-08-07 eCollection Date: 2024-10-28 DOI: 10.1021/cbmi.4c00040

Nycol M Cotto, Neeraj Chauhan, Benilde Adriano, Deepak S Chauhan, Marco Cabrera, Subhash C Chauhan, Murali M Yallapu

{"title":"Milk Exosome-Glow Nanosystem for Cancer Cellular and Tissue Bioimaging.","authors":"Nycol M Cotto, Neeraj Chauhan, Benilde Adriano, Deepak S Chauhan, Marco Cabrera, Subhash C Chauhan, Murali M Yallapu","doi":"10.1021/cbmi.4c00040","DOIUrl":"10.1021/cbmi.4c00040","url":null,"abstract":"Milk-derived exosomes are widely used for diagnosis, delivery, imaging, and theranostic applications. Near-Infrared (NIR) based fluorescence bioimaging is an attractive and safer technique that is used for clinical applications. However, almost all NIR imaging agents tend to have poor photostability, short half-life, nonspecific protein binding, and concentration-dependent aggregation(s). Therefore, there is an unmet clinical need to develop newer and safer modalities to package and deliver NIR imaging agents. Bovine milk exosomes are natural, biocompatible, safe, and efficient nanocarriers that facilitate the delivery of micro- and macromolecules. Herein, we developed an exosome-based NIR dye loaded nanoimaging formulation that offers improved solubility and photostability of NIR dye. Following the acetic acid based extracellular vesicle (EV) treatment method, we extracted the bovine milk exosomes from a variety of pasteurized grade milk. The EVs were screened for their physicochemical properties such as particle size and concentration and zeta potential. The stability of these exosomes was also determined under different conditions, including storage temperatures, pH, and salt concentrations. Next, indocyanine green, a model NIR dye was loaded into these exosomes (Exo-Glow) via a sonication method and further assessed for their improved fluorescence intensity and photostability using an IVIS imaging system. Initial screening suggested that size of the selected bovine milk exosomes was ∼100-135 nm with an average particle concentration of 5.8 × 102 particles/mL. Exo-Glow further demonstrated higher fluorescence intensity in cancer cells and tissues when compared to free dye. These results showed that Exo-Glow has the potential to serve as a safer NIR imaging tool for cancer cells/tissues.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 10","pages":"711-720"},"PeriodicalIF":0.0,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11522989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142559455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Milk Exosome-Glow Nanosystem for Cancer Cellular and Tissue Bioimaging 用于癌症细胞和组织生物成像的牛奶外泌体-光纳米系统

Chemical & Biomedical Imaging Pub Date : 2024-08-07 DOI: 10.1021/cbmi.4c0004010.1021/cbmi.4c00040

Nycol M. Cotto, Neeraj Chauhan, Benilde Adriano, Deepak S. Chauhan, Marco Cabrera, Subhash C. Chauhan and Murali M. Yallapu*,

{"title":"Milk Exosome-Glow Nanosystem for Cancer Cellular and Tissue Bioimaging","authors":"Nycol M. Cotto, Neeraj Chauhan, Benilde Adriano, Deepak S. Chauhan, Marco Cabrera, Subhash C. Chauhan and Murali M. Yallapu*, ","doi":"10.1021/cbmi.4c0004010.1021/cbmi.4c00040","DOIUrl":"https://doi.org/10.1021/cbmi.4c00040https://doi.org/10.1021/cbmi.4c00040","url":null,"abstract":"Milk-derived exosomes are widely used for diagnosis, delivery, imaging, and theranostic applications. Near-Infrared (NIR) based fluorescence bioimaging is an attractive and safer technique that is used for clinical applications. However, almost all NIR imaging agents tend to have poor photostability, short half-life, nonspecific protein binding, and concentration-dependent aggregation(s). Therefore, there is an unmet clinical need to develop newer and safer modalities to package and deliver NIR imaging agents. Bovine milk exosomes are natural, biocompatible, safe, and efficient nanocarriers that facilitate the delivery of micro- and macromolecules. Herein, we developed an exosome-based NIR dye loaded nanoimaging formulation that offers improved solubility and photostability of NIR dye. Following the acetic acid based extracellular vesicle (EV) treatment method, we extracted the bovine milk exosomes from a variety of pasteurized grade milk. The EVs were screened for their physicochemical properties such as particle size and concentration and zeta potential. The stability of these exosomes was also determined under different conditions, including storage temperatures, pH, and salt concentrations. Next, indocyanine green, a model NIR dye was loaded into these exosomes (Exo-Glow) via a sonication method and further assessed for their improved fluorescence intensity and photostability using an IVIS imaging system. Initial screening suggested that size of the selected bovine milk exosomes was ∼100–135 nm with an average particle concentration of 5.8 × 102 particles/mL. Exo-Glow further demonstrated higher fluorescence intensity in cancer cells and tissues when compared to free dye. These results showed that Exo-Glow has the potential to serve as a safer NIR imaging tool for cancer cells/tissues.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 10","pages":"711–720 711–720"},"PeriodicalIF":0.0,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142517319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0