Chemical & Biomedical Imaging最新文献_第10页

Microtopography-Induced Nuclear Deformation Triggers Chromatin Reorganization and Cytoskeleton Remodeling 微形貌诱导的核变形触发染色质重组和细胞骨架重塑

Chemical & Biomedical Imaging Pub Date : 2024-05-10 DOI: 10.1021/cbmi.4c0003510.1021/cbmi.4c00035

Hong Liang, Ya-Jun Wang, Yixin Liu, Wei Liu, Baohong Liu* and Yan-Jun Liu*,

{"title":"Microtopography-Induced Nuclear Deformation Triggers Chromatin Reorganization and Cytoskeleton Remodeling","authors":"Hong Liang, Ya-Jun Wang, Yixin Liu, Wei Liu, Baohong Liu* and Yan-Jun Liu*, ","doi":"10.1021/cbmi.4c0003510.1021/cbmi.4c00035","DOIUrl":"https://doi.org/10.1021/cbmi.4c00035https://doi.org/10.1021/cbmi.4c00035","url":null,"abstract":"Cells can adapt to diverse topographical substrates through contact guidance, which regulates the cellular and nuclear morphologies and functions. How adaptive deformation of the cell body and nucleus coordinates to protect genetic material within mechanical microenvironments remains poorly understood. In this study, we engineered micrometer-level narrow-spacing micropillars to mimic constricted extracellular topographies in vivo, enabling us to explore variances in the nuclear architecture, cytoskeleton distribution, and chromatin conformation. The results showed that the area and volume of cell nuclei were distinctly smaller on micropillar topography. Actin and vimentin densely encapsulated the micropillars surrounding the nucleus, effectively segregating it from the micropillars. Additionally, nucleo-cytoskeleton lamin A/C exhibited a polarized distribution at the protrusion of the deformed nuclei. Notably, the degree of heterochromatin was altered in response to significant nuclear deformation, leading to a downregulation trend in H3K9me3 expression. These findings suggest that mechanical constraints imposed by microtopography profoundly influence cell behaviors, providing insights into disease diagnosis and therapeutic interventions in vivo.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 7","pages":"481–489 481–489"},"PeriodicalIF":0.0,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00035","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141959308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescent Molecular Rotors Quantify an Adjuvant-Induced Softening of Plant Wax 荧光分子旋转仪量化佐剂诱导的植物蜡软化现象

Chemical & Biomedical Imaging Pub Date : 2024-05-07 DOI: 10.1021/cbmi.4c00005

Petr S. Sherin*, Markus Rueckel* and Marina K. Kuimova*,

{"title":"Fluorescent Molecular Rotors Quantify an Adjuvant-Induced Softening of Plant Wax","authors":"Petr S. Sherin*, Markus Rueckel* and Marina K. Kuimova*, ","doi":"10.1021/cbmi.4c00005","DOIUrl":"10.1021/cbmi.4c00005","url":null,"abstract":"Epicuticular wax is the outmost layer of plant leaves that protects them from desiccation and penetration of harmful reagents. There is an intense industrial effort in the development of softening agents, adjuvants, that can adjust the permeability of the wax toward pesticides and, thus, play an important role in sustainable agriculture. However, mechanistic understanding of the structure and dynamic properties within the plant wax, particularly upon the application of adjuvants, is currently lacking. In this work, we demonstrate that fluorescence lifetime imaging microscopy (FLIM) combined with molecular rotors, fluorescent probes sensitive to viscosity, can directly probe the microviscosity of amorphous and crystalline phases of model plant wax layers. Moreover, this approach is able to quantify the changes in viscosity in both phases upon the addition of water and adjuvant solutions on top of the wax. We show that water permeation mostly perturbs the crystalline phase of the wax, while our chosen adjuvant, Plurafac LF431, mainly softens the amorphous phase of the wax. Our technique provides a facile and quantitative way to monitor dynamic properties within plant waxes with diffraction-limited resolution and reveals the effect of organic substances on wax structure and rigidity, crucial for designing next-generation agents to improve agricultural efficiency.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 6","pages":"453–461"},"PeriodicalIF":0.0,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141003966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing Open-World Bacterial Raman Spectra Identification by Feature Regularization for Improved Resilience against Unknown Classes 通过特征正则化增强开放世界细菌拉曼光谱识别能力，提高对未知类别的复原力

Chemical & Biomedical Imaging Pub Date : 2024-05-06 DOI: 10.1021/cbmi.4c00007

Yaroslav Balytskyi*, Nataliia Kalashnyk, Inna Hubenko, Alina Balytska and Kelly McNear,

{"title":"Enhancing Open-World Bacterial Raman Spectra Identification by Feature Regularization for Improved Resilience against Unknown Classes","authors":"Yaroslav Balytskyi*, Nataliia Kalashnyk, Inna Hubenko, Alina Balytska and Kelly McNear, ","doi":"10.1021/cbmi.4c00007","DOIUrl":"https://doi.org/10.1021/cbmi.4c00007","url":null,"abstract":"The combination of deep learning techniques and Raman spectroscopy shows great potential offering precise and prompt identification of pathogenic bacteria in clinical settings. However, the traditional closed-set classification approaches assume that all test samples belong to one of the known pathogens, and their applicability is limited since the clinical environment is inherently unpredictable and dynamic, unknown, or emerging pathogens may not be included in the available catalogs. We demonstrate that the current state-of-the-art neural networks identifying pathogens through Raman spectra are vulnerable to unknown inputs, resulting in an uncontrollable false positive rate. To address this issue, first we developed an ensemble of ResNet architectures combined with the attention mechanism that achieves a 30-isolate accuracy of 87.8 ± 0.1%. Second, through the integration of feature regularization by the Objectosphere loss function, our model both achieves high accuracy in identifying known pathogens from the catalog and effectively separates unknown samples drastically reducing the false positive rate. Finally, the proposed feature regularization method during training significantly enhances the performance of out-of-distribution detectors during the inference phase improving the reliability of the detection of unknown classes. Our algorithm for Raman spectroscopy empowers the identification of previously unknown, uncataloged, and emerging pathogens ensuring adaptability to future pathogens that may surface. Moreover, it can be extended to enhance open-set medical image classification, bolstering its reliability in dynamic operational settings.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 6","pages":"442–452"},"PeriodicalIF":0.0,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141474776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence Imaging Using Deep-Red Indocyanine Blue, a Complementary Partner for Near-Infrared Indocyanine Green 使用深红靛蓝（近红外靛蓝绿的互补搭档）进行荧光成像

Chemical & Biomedical Imaging Pub Date : 2024-05-02 DOI: 10.1021/cbmi.4c00008

Rananjaya S. Gamage, and , Bradley D. Smith*,

{"title":"Fluorescence Imaging Using Deep-Red Indocyanine Blue, a Complementary Partner for Near-Infrared Indocyanine Green","authors":"Rananjaya S. Gamage,  and , Bradley D. Smith*, ","doi":"10.1021/cbmi.4c00008","DOIUrl":"10.1021/cbmi.4c00008","url":null,"abstract":"Indocyanine Blue (ICB) is the deep-red pentamethine analogue of the widely used clinical near-infrared heptamethine cyanine dye Indocyanine Green (ICG). The two fluorophores have the same number of functional groups and molecular charge and vary only by a single vinylene unit in the polymethine chain, which produces a predictable difference in spectral and physicochemical properties. We find that the two dyes can be employed as a complementary pair in diverse types of fundamental and applied fluorescence imaging experiments. A fundamental fluorescence spectroscopy study used ICB and ICG to test a recently proposed Förster Resonance Energy Transfer (FRET) mechanism for enhanced fluorescence brightness in heavy water (D2O). The results support two important corollaries of the proposal: (a) the strategy of using heavy water to increase the brightness of fluorescent dyes for microscopy or imaging is most effective when the dye emission band is above 650 nm, and (b) the magnitude of the heavy water florescence enhancement effect for near-infrared ICG is substantially diminished when the ICG surface is dehydrated due to binding by albumin protein. Two applied fluorescence imaging studies demonstrated how deep-red ICB can be combined with a near-infrared fluorophore for paired agent imaging in the same living subject. One study used dual-channel mouse imaging to visualize increased blood flow in a model of inflamed tissue, and a second mouse tumor imaging study simultaneously visualized the vasculature and cancerous tissue in separate fluorescence channels. The results suggest that ICB and ICG can be incorporated within multicolor fluorescence imaging methods for perfusion imaging and hemodynamic characterization of a wide range of diseases.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 5","pages":"384–397"},"PeriodicalIF":0.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141018311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptide-Based Turn-On Fluorescent Probes for Highly Specific Detection of Survivin Protein in the Cancer Cells 用于高度特异性检测癌细胞中 Survivin 蛋白的肽基开启荧光探针

Chemical & Biomedical Imaging Pub Date : 2024-05-01 DOI: 10.1021/cbmi.4c00017

Takeshi Fuchigami*, Tomoe Nakayama, Yusuke Miyanari, Iori Nozaki, Natsumi Ishikawa, Ayako Tagawa, Sakura Yoshida, Masayuki Munekane, Morio Nakayama and Kazuma Ogawa,

{"title":"Peptide-Based Turn-On Fluorescent Probes for Highly Specific Detection of Survivin Protein in the Cancer Cells","authors":"Takeshi Fuchigami*, Tomoe Nakayama, Yusuke Miyanari, Iori Nozaki, Natsumi Ishikawa, Ayako Tagawa, Sakura Yoshida, Masayuki Munekane, Morio Nakayama and Kazuma Ogawa, ","doi":"10.1021/cbmi.4c00017","DOIUrl":"10.1021/cbmi.4c00017","url":null,"abstract":"Survivin is highly expressed in most human cancers, making it a promising target for cancer diagnosis and treatment. In this study, we developed peptide probes consisting of Bor65–75, a high-affinity survivin-binding peptide, and a survivin protein segment using peptide linkers as survivin-sensitive fluorescent probes (SSFPs). All conjugates were attached to 5(6)-carboxyfluorescein (FAM) at the C-terminal as a fluorophore and to 4((4(dimethylamino)phenyl)azo)benzoic acid (DABCYL) at the N-terminal as a quencher. Fluorescence (or Förster) resonance energy transfer (FRET) quenching via intramolecular binding of Bor65–75 with survivin protein segment could be diminished by the approach of survivin to SSFPs, which dissociate Bor65–75 from SSPF and increased the distance between FAM and DABCYL. A binding assay using recombinant human survivin protein (rSurvivin) demonstrated moderate to high affinity of SSFPs for survivin (dissociation constants (Kd) = 121–1740 nM). Although the SSFPs (0.5 μM) had almost no fluorescence under baseline conditions, a dose-dependent increase in fluorescence intensity was observed in the presence of rSurvivin (0.1–2.0 μM). In particular, the proline-rich SSFP (SSFP5) showed the highest (2.7-fold) fluorescence induction at 2.0 μM survivin compared to the signals in the absence of survivin. Confocal fluorescence imaging demonstrated that SSFP5 exhibited clear fluorescence signals in survivin-positive MDA-MB-231 cells, whereas no marked fluorescence signals were observed in survivin-negative MCF-10A cells. Collectively, these results suggest that SSFPs can be used as survivin-specific FRET imaging probes.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 5","pages":"374–383"},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141035076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Progress in Molecular Probes for Imaging of Acute Kidney Injury 急性肾损伤成像分子探针的最新进展

Chemical & Biomedical Imaging Pub Date : 2024-05-01 DOI: 10.1021/cbmi.4c0002410.1021/cbmi.4c00024

Muqadas Sitara, Wangning Zhang, Han Gao, Jiwei Li* and Jiangwei Tian*,

{"title":"Recent Progress in Molecular Probes for Imaging of Acute Kidney Injury","authors":"Muqadas Sitara, Wangning Zhang, Han Gao, Jiwei Li* and Jiangwei Tian*, ","doi":"10.1021/cbmi.4c0002410.1021/cbmi.4c00024","DOIUrl":"https://doi.org/10.1021/cbmi.4c00024https://doi.org/10.1021/cbmi.4c00024","url":null,"abstract":"Acute kidney injury (AKI), a prevalent and complex clinical condition associated with elevated risks of morbidity and mortality, necessitates the meticulous detection and monitoring of kidney damage globally. Biomedicine has shown keen interest in molecular probes and detectors for AKI due to their sensitivity, rapidity, and cost-effectiveness. Bioimaging technologies play a significant role in identifying and quantifying AKI indicators, enhancing diagnostic approaches, and potentially refining clinical therapies for immediate injury control. Molecular probes serve as valuable tools for drug screening, uncovering renoprotective components, signaling pathways, and the nephrotoxic effects of drugs. This review comprehensively summarizes the latest advancements in molecular probes, emphasizing their exceptional efficacy in various characteristics, including renal cleanability, multichannel detection capability, near-infrared-II responsiveness, and reactivity toward reactive oxygen species. These probes offer enhanced benefits for assessing kidney damage and evaluating the therapeutic effects of medications while simultaneously reducing toxic effects. Additionally, the review delves into future potentials and challenges in this field, aiming to inspire the development and enhancement of molecular bioimaging for the early diagnosis and treatment of kidney diseases.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 8","pages":"526–541 526–541"},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142075704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling the Thermoelastic Sample Response for Subdiffraction Infrared Spectroscopic Imaging 为亚衍射红外光谱成像建立热弹性样品响应模型

Chemical & Biomedical Imaging Pub Date : 2024-04-29 DOI: 10.1021/cbmi.4c00018

Seth Kenkel, and , Rohit Bhargava*,

{"title":"Modeling the Thermoelastic Sample Response for Subdiffraction Infrared Spectroscopic Imaging","authors":"Seth Kenkel,  and , Rohit Bhargava*, ","doi":"10.1021/cbmi.4c00018","DOIUrl":"10.1021/cbmi.4c00018","url":null,"abstract":"There is significant and increasing interest in using the photothermal effect to record infrared (IR) absorption spectra localized to volumes that are considerably smaller than the wavelength of excitation, i.e., subdiffraction imaging. As opposed to conventional IR microscopy, in which absorption and scattering of the illuminating light is measured, subdiffraction imaging can be achieved through detection of the sample’s thermal response to IR absorption-induced heating. While this relationship has been examined by a variety of coarse-grained models, a generalized analysis of the dependence of temperature and surface deformation arising from an absorber below the surface has not been reported. Here, we present an analytical model to understand a sample’s thermoelastic response in photothermal measurements. The model shows important dependence of the ability to record subdiffraction data on modulation frequency of exciting light, limitations imposed by optical sensing, and the potential to discern location of objects ultimately limited by noise and sharpness of the detecting mechanism. This foundational analysis should allow for better modeling, understanding, and harnessing of the relationship between absorption and sample response that underlies IR photothermal measurements.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 6","pages":"413–421"},"PeriodicalIF":0.0,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11200252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141472566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-Time Tracking of Vesicles in Living Cells Reveals That Tau-Hyperphosphorylation Suppresses Unidirectional Transport by Motor Proteins 实时跟踪活细胞中的囊泡发现 Tau 过度磷酸化抑制了马达蛋白的单向运输

Chemical & Biomedical Imaging Pub Date : 2024-04-23 DOI: 10.1021/cbmi.4c00016

Eunsang Lee, Donghee Kim, Yo Han Song, Kyujin Shin, Sanggeun Song, Minho Lee, Yeongchang Goh, Mi Hee Lim, Ji-Hyun Kim, Jaeyoung Sung* and Kang Taek Lee*,

{"title":"Real-Time Tracking of Vesicles in Living Cells Reveals That Tau-Hyperphosphorylation Suppresses Unidirectional Transport by Motor Proteins","authors":"Eunsang Lee, Donghee Kim, Yo Han Song, Kyujin Shin, Sanggeun Song, Minho Lee, Yeongchang Goh, Mi Hee Lim, Ji-Hyun Kim, Jaeyoung Sung* and Kang Taek Lee*, ","doi":"10.1021/cbmi.4c00016","DOIUrl":"https://doi.org/10.1021/cbmi.4c00016","url":null,"abstract":"Synaptic vesicle transport by motor proteins along microtubules is a crucially active process underlying neuronal communication. It is known that microtubules are destabilized by tau-hyperphosphorylation, which causes tau proteins to detach from microtubules and form neurofibril tangles. However, how tau-phosphorylation affects the transport dynamics of motor proteins on the microtubule remains unknown. Here, we discover that the long-distance unidirectional motion of vesicle-motor protein multiplexes (VMPMs) in living cells is suppressed under tau-hyperphosphorylation, with the consequent loss of fast vesicle-transport along the microtubule. The VMPMs in hyperphosphorylated cells exhibit seemingly bidirectional random motion, with dynamic properties far different from those of VMPM motion in normal cells. We establish a parsimonious physicochemical model of VMPM’s active motion that provides a unified, quantitative explanation and predictions for our experimental results. Our analysis reveals that, under hyperphosphorylation conditions, motor protein multiplexes have both static and dynamic motility fluctuations. The loss of fast vesicle-transport along the microtubule can be a mechanism of neurodegenerative disorders associated with tau-hyperphosphorylation.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 5","pages":"362–373"},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141156243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multivariate Analysis of Individual Bacterial Outer Membrane Vesicles Using Fluorescence Microscopy 利用荧光显微镜对单个细菌外膜囊泡进行多元分析

Chemical & Biomedical Imaging Pub Date : 2024-04-19 DOI: 10.1021/cbmi.4c00014

Aarshi N. Singh, Justin B Nice, Meishan Wu, Angela C. Brown and Nathan J. Wittenberg*,

{"title":"Multivariate Analysis of Individual Bacterial Outer Membrane Vesicles Using Fluorescence Microscopy","authors":"Aarshi N. Singh, Justin B Nice, Meishan Wu, Angela C. Brown and Nathan J. Wittenberg*, ","doi":"10.1021/cbmi.4c00014","DOIUrl":"10.1021/cbmi.4c00014","url":null,"abstract":"Gram-negative bacteria produce outer membrane vesicles (OMVs) that play a critical role in cell–cell communication and virulence. OMVs have emerged as promising therapeutic agents for various biological applications such as vaccines and targeted drug delivery. However, the full potential of OMVs is currently constrained by inherent heterogeneities, such as size and cargo differences, and traditional ensemble assays are limited in their ability to reveal OMV heterogeneity. To overcome this issue, we devised an innovative approach enabling the identification of various characteristics of individual OMVs. This method, employing fluorescence microscopy, facilitates the detection of variations in size and surface markers. To demonstrate our method, we utilize the oral bacterium Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) which produces OMVs with a bimodal size distribution. As part of its virulence, A. actinomycetemcomitans secretes leukotoxin (LtxA) in two forms: soluble and surface associated with the OMVs. We observed a correlation between the size and toxin presence where larger OMVs were much more likely to possess LtxA compared to the smaller OMVs. In addition, we noted that, among the smallest OMVs (<100 nm diameter), the fractions that are toxin positive range from 0 to 30%, while the largest OMVs (>200 nm diameter) are between 70 and 100% toxin positive.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 5","pages":"352–361"},"PeriodicalIF":0.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140684259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements and Practical Considerations for Biophysical Research: Navigating the Challenges and Future of Super-resolution Microscopy 生物物理研究的进展和实际考虑因素：驾驭超分辨率显微镜的挑战与未来

Chemical & Biomedical Imaging Pub Date : 2024-04-19 DOI: 10.1021/cbmi.4c00019

Huanhuan Chen, Guangjie Yan, Meng-Hsuan Wen, Kameron N. Brooks, Yuteng Zhang, Pei-San Huang and Tai-Yen Chen*,

{"title":"Advancements and Practical Considerations for Biophysical Research: Navigating the Challenges and Future of Super-resolution Microscopy","authors":"Huanhuan Chen, Guangjie Yan, Meng-Hsuan Wen, Kameron N. Brooks, Yuteng Zhang, Pei-San Huang and Tai-Yen Chen*, ","doi":"10.1021/cbmi.4c00019","DOIUrl":"10.1021/cbmi.4c00019","url":null,"abstract":"The introduction of super-resolution microscopy (SRM) has significantly advanced our understanding of cellular and molecular dynamics, offering a detailed view previously beyond our reach. Implementing SRM in biophysical research, however, presents numerous challenges. This review addresses the crucial aspects of utilizing SRM effectively, from selecting appropriate fluorophores and preparing samples to analyzing complex data sets. We explore recent technological advancements and methodological improvements that enhance the capabilities of SRM. Emphasizing the integration of SRM with other analytical methods, we aim to overcome inherent limitations and expand the scope of biological insights achievable. By providing a comprehensive guide for choosing the most suitable SRM methods based on specific research objectives, we aim to empower researchers to explore complex biological processes with enhanced precision and clarity, thereby advancing the frontiers of biophysical research.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"2 5","pages":"331–344"},"PeriodicalIF":0.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.4c00019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140683836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0