Chemical & Biomedical Imaging最新文献_第9页

Advances in Small-Molecule Fluorescent pH Probes for Monitoring Mitophagy 用于监测有丝分裂的小分子 pH 值荧光探针的研究进展

Chemical & Biomedical Imaging Pub Date : 2023-08-02 DOI: 10.1021/cbmi.3c00070

Yurui Liu, Duoteng Zhang, Yunwei Qu, Fang Tang, Hui Wang, Aixiang Ding* and Lin Li*,

{"title":"Advances in Small-Molecule Fluorescent pH Probes for Monitoring Mitophagy","authors":"Yurui Liu, Duoteng Zhang, Yunwei Qu, Fang Tang, Hui Wang, Aixiang Ding* and Lin Li*, ","doi":"10.1021/cbmi.3c00070","DOIUrl":"10.1021/cbmi.3c00070","url":null,"abstract":"Mitochondria play a crucial role in regulating cellular energy homeostasis and cell death, making them essential organelles. Maintaining proper cellular functions relies on the removal of damaged mitochondria through a process called mitophagy. Mitophagy is associated with changes in the pH value and has implications for numerous diseases. To effectively monitor mitophagy, fluorescent probes that exhibit high selectivity and sensitivity based on pH detection have emerged as powerful tools. In this review, we present recent advancements in the monitoring of mitophagy using small-molecule fluorescence pH probes. We focus on various sensing mechanisms employed by these probes, including intramolecular charge transfer (ICT), fluorescence resonance energy transfer (FRET), through bond energy transfer (TBET), and photoelectron transfer (PET). Additionally, we discuss disease models used for studying mitophagy and summarize the design requirements for small-molecule fluorescent pH probes suitable for monitoring the mitophagy process. Lastly, we highlight the remaining challenges in this field and propose potential directions for the future development of mitophagy probes.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.3c00070","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131050061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facet-Dependent Photoelectrochemistry on Single Crystal Organic–Inorganic Halide Perovskite Electrodes 单晶有机-无机卤化物钙钛矿电极的面依赖性光电化学研究

Chemical & Biomedical Imaging Pub Date : 2023-08-02 DOI: 10.1021/cbmi.3c00069

Partha Saha, Md. Maksudur Rahman, Chloe L. Tolbert and Caleb M. Hill*,

{"title":"Facet-Dependent Photoelectrochemistry on Single Crystal Organic–Inorganic Halide Perovskite Electrodes","authors":"Partha Saha, Md. Maksudur Rahman, Chloe L. Tolbert and Caleb M. Hill*, ","doi":"10.1021/cbmi.3c00069","DOIUrl":"10.1021/cbmi.3c00069","url":null,"abstract":"Organometallic halide perovskites have garnered significant attention in various fields of material science, particularly solar energy conversion, due to their desirable optoelectronic properties and compatibility with scalable fabrication techniques. It is often unclear, however, how carrier generation and transport within complex polycrystalline films are influenced by variations in local structure. Elucidating how distinct structural motifs within these heterogeneous systems affect behavior could help guide the continued improvement of perovskite-based solar cells. Here, we present studies applying scanning electron microscopy (SECCM) to map solar energy harvesting within well-defined model systems of organometallic halide perovskites. Methylammonium lead bromide (MAPbBr3) single crystals were prepared via a low-temperature solution-based route, and their photoelectrochemical properties were mapped via SECCM using p-benzoquinone (BQ) in dichloromethane as a redox mediator. Correlated SECCM mapping and electron microscopy studies enabled facet-to-facet variations in photoelectrochemical performance to be revealed and carrier transport lengths to be evaluated. The photoelectrochemical behavior observed within individual single crystals was quite heterogeneous, attributable to local variations in crystal structure/orientations, intrafacet junctions, and the presence of other structural defects. These observations underscore the significance of controlling the microstructure of single perovskite crystals, presenting a promising avenue for further enhancement of perovskite-based solar cells.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/87/f6/im3c00069.PMC10467489.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10128083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Next Generation of Mass Spectrometry Imaging: from Micrometer to Subcellular Resolution 下一代质谱成像:从微米到亚细胞分辨率

Chemical & Biomedical Imaging Pub Date : 2023-07-24 DOI: 10.1021/cbmi.3c00061

Lei Xing, Cong-Lin Zhao, Han-Zhang Mou, JianBin Pan, Bin Kang*, Hong-Yuan Chen and Jing-Juan Xu*,

引用次数: 0

Supertemporal Resolution Imaging of Membrane Potential via Stroboscopic Microscopy 频闪显微镜膜电位的超时间分辨率成像

Chemical & Biomedical Imaging Pub Date : 2023-07-20 DOI: 10.1021/cbmi.3c00054

Luxin Peng, and , Peng Zou*,

{"title":"Supertemporal Resolution Imaging of Membrane Potential via Stroboscopic Microscopy","authors":"Luxin Peng,  and , Peng Zou*, ","doi":"10.1021/cbmi.3c00054","DOIUrl":"https://doi.org/10.1021/cbmi.3c00054","url":null,"abstract":"Membrane potential and its fluctuation are fundamental biophysical phenomena essential to cellular activities and functions. Compared to traditional electrode-based techniques, the optical recording via developed genetically encoded voltage indicators (GEVIs) offers a combination of noninvasiveness, high spatial resolution, and increased measurement throughput. However, its application is limited by the insufficient acquisition rate and time accuracy of the camera. Here we design and apply a stroboscopic illumination scheme to boost the temporal resolution of voltage imaging, while simultaneously eliminating the artifacts caused by nonsynchronized exposure in the rolling-shutter mode. We demonstrate that commonly used GEVIs are compatible with stroboscopic voltage imaging (SVI), and our SVI scheme offers a 5-fold faster acquisition frame rate than that of conventional continuous illumination. The GEVIs tested maintain high sensitivities in the SVI mode, supporting faithful reports of intracellular depolarization waveform and intercellular gap junction-mediated depolarization coupling in human embryonic kidney 293T (HEK 293T) cell populations. SVI allows resolving the action potential (AP) waveform with less distortion and mapping action potential initiation and propagation dynamics in cultured neurons in kilohertz, beyond the restriction from the camera in the field of view.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.3c00054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49768285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Benzothiazole-Based Fluorescent Probe as a Simple and Effective Platform for Functional Mitochondria Imaging 基于苯并噻唑的荧光探针作为功能线粒体成像的简单有效平台

Chemical & Biomedical Imaging Pub Date : 2023-07-10 DOI: 10.1021/cbmi.3c00055

Kyeong-Im Hong, Younghun Kim, Jeong Heon Lee, Kang Ho Chu and Woo-Dong Jang*,

{"title":"Benzothiazole-Based Fluorescent Probe as a Simple and Effective Platform for Functional Mitochondria Imaging","authors":"Kyeong-Im Hong, Younghun Kim, Jeong Heon Lee, Kang Ho Chu and Woo-Dong Jang*, ","doi":"10.1021/cbmi.3c00055","DOIUrl":"https://doi.org/10.1021/cbmi.3c00055","url":null,"abstract":"Mitochondrial imaging is crucial for studying disease pathogenesis and diagnosis. However, commercially available mitochondria-specific fluorescent probes are limited due to the difficulty in modifying cationic probes for functional bioimaging of mitochondria. To address this, we prepared a hydroxythiophene-conjugated benzothiazole (BzT-OH) as a platform for the design of functional fluorescent probes. Two probes, BzT-OAc and BzT-OAcryl, were synthesized by substituting the hydroxy group in BzT-OH with acetate and acrylate groups, respectively. BzT-OAc demonstrated blue fluorescence that shifted to green emission after enzymatic cleavage of the acetate group, allowing for monitoring of endogenous esterase activity. BzT-OAcryl showed high selectivity for cysteine at pH > 8.0, owing to its pH-responsive property, and could detect pH perturbations caused by mitochondrial dysfunction. Both probes exhibited high biocompatibility, quantum yield, and large Stokes shifts in mitochondria. BzT-OH can be easily conjugated with other functional groups and substrates of various enzymes for designing fluorescent probes for functional mitochondrial imaging. The photofunctional property of the probe can be changed due to the involvement of the hydroxyl group in the excited state intermolecular proton transfer.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.3c00055","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49768601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Application of Bio-orthogonality for In Vivo Animal Imaging 生物正交性在动物体内成像中的应用

Chemical & Biomedical Imaging Pub Date : 2023-07-07 DOI: 10.1021/cbmi.3c00033

Jun Yang, Biyue Zhu and Chongzhao Ran*,

引用次数: 1

Exploring Lignification Complexity in Plant Cell Walls with Airyscan Super-resolution Microscopy and Bioorthogonal Chemistry 利用airscan超分辨显微镜和生物正交化学研究植物细胞壁木质素化的复杂性

Chemical & Biomedical Imaging Pub Date : 2023-07-06 DOI: 10.1021/cbmi.3c00052

Clémence Simon, Oriane Morel, Godfrey Neutelings, Fabien Baldacci-Cresp, Marie Baucher, Corentin Spriet, Christophe Biot, Simon Hawkins and Cédric Lion*,

{"title":"Exploring Lignification Complexity in Plant Cell Walls with Airyscan Super-resolution Microscopy and Bioorthogonal Chemistry","authors":"Clémence Simon, Oriane Morel, Godfrey Neutelings, Fabien Baldacci-Cresp, Marie Baucher, Corentin Spriet, Christophe Biot, Simon Hawkins and Cédric Lion*, ","doi":"10.1021/cbmi.3c00052","DOIUrl":"https://doi.org/10.1021/cbmi.3c00052","url":null,"abstract":"In this paper, we present the use of multiplex click/bioorthogonal chemistry combined with super-resolution Airyscan microscopy to track biomolecules in living systems with a focus on studying lignin formation in plant cell walls. While laser scanning confocal microscopy (LSCM) provided insights into the tissue-scale dynamics of lignin formation and distribution in our previous reports, its limited resolution precluded an in-depth analysis of lignin composition at the unique cell wall or substructure level. To overcome this limitation, we explored the use of Airyscan microscopy, which, among the super-resolution techniques available, offers an optimal balance between performance, cost, accessibility, and ease of implementation. Our study demonstrates that a triple labeling strategy using copper-catalyzed azide–alkyne cycloaddition (CuAAC), strain-promoted azide–alkyne cycloaddition (SPAAC), and inverse electronic-demand Diels–Alder cycloaddition (IEDDA) to label modified lignin metabolic precursors can be combined with Airyscan microscopy to reveal the zones of active lignification at the single cell level with improved sensitivity and resolution. This approach enables insights into the lignin composition in wall substructures, such as pits or in wall layers that are otherwise not distinguishable by classical LSCM. Our work emphasizes the importance of studying lignin formation in plant cell walls and demonstrates the potential of combining bioorthogonal chemistry and super-resolution microscopy techniques for studying biomolecules in living systems.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.3c00052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49768340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Bioimaging Story of AIEgens 艾庚的生物成像故事

Chemical & Biomedical Imaging Pub Date : 2023-07-03 DOI: 10.1021/cbmi.3c00056

Siyuan Wang, Kun Zhou, Xinyan Lyu, Haowen Li, Zijie Qiu*, Zheng Zhao* and Ben Zhong Tang*,

引用次数: 0

Electrochemiluminescence Imaging of Cellular Contact Guidance on Microfabricated Substrates 微基板上细胞接触引导的电化学发光成像

Chemical & Biomedical Imaging Pub Date : 2023-06-29 DOI: 10.1021/cbmi.3c00066

Lurong Ding, Ping Zhou*, Yajuan Yan and Bin Su*,

{"title":"Electrochemiluminescence Imaging of Cellular Contact Guidance on Microfabricated Substrates","authors":"Lurong Ding, Ping Zhou*, Yajuan Yan and Bin Su*, ","doi":"10.1021/cbmi.3c00066","DOIUrl":"https://doi.org/10.1021/cbmi.3c00066","url":null,"abstract":"Cells tend to align and move by following anisotropic topographical cues, namely the phenomenon known as contact guidance─an essential step in cell alignment, adhesion, and migration. The effect of topographical cues on individual cells has been investigated extensively, but that on cell aggregates still remains to be fully understood. Considering the high surface sensitivity of electrochemiluminescence (ECL) microscopy, it was used in this work to explore the impact of surface topography on cell behaviors. First, we studied the variations of cell–matrix adhesions of cells cultured on different topographical features. Both fibroblast-like and epithelial cells plated on microgrooved electrodes exhibited obvious contact guidance behavior. Then, the effect of surface topography on cellular collective migration was investigated. Topographic cues would be a barrier for cell migration if the orientation of microgrooves was perpendicular to the direction of migration; otherwise, it would be a helper. Finally, it was found that relaxation of cytoskeleton contractility or reduction in adhesion density could weaken the directed migration of leading cells, because the alteration of migration directionality was retarded. In contrast, such interactions were lost on the contact guidance response of follower cells, as they still aligned by following the topographic cues.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/cbmi.3c00066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49768483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Switchable and Functional Fluorophores for Multidimensional Single-Molecule Localization Microscopy 用于多维单分子定位显微镜的可切换和功能荧光团

Chemical & Biomedical Imaging Pub Date : 2023-06-28 DOI: 10.1021/cbmi.3c00045

Yunshu Liu, Md Abul Shahid, Hongjing Mao, Jiahui Chen, Michael Waddington, Ki-Hee Song* and Yang Zhang*,

{"title":"Switchable and Functional Fluorophores for Multidimensional Single-Molecule Localization Microscopy","authors":"Yunshu Liu, Md Abul Shahid, Hongjing Mao, Jiahui Chen, Michael Waddington, Ki-Hee Song* and Yang Zhang*, ","doi":"10.1021/cbmi.3c00045","DOIUrl":"10.1021/cbmi.3c00045","url":null,"abstract":"Multidimensional single-molecule localization microscopy (mSMLM) represents a paradigm shift in the realm of super-resolution microscopy techniques. It affords the simultaneous detection of single-molecule spatial locations at the nanoscale and functional information by interrogating the emission properties of switchable fluorophores. The latter is finely tuned to report its local environment through carefully manipulated laser illumination and single-molecule detection strategies. This Perspective highlights recent strides in mSMLM with a focus on fluorophore designs and their integration into mSMLM imaging systems. Particular interests are the accomplishments in simultaneous multiplexed super-resolution imaging, nanoscale polarity and hydrophobicity mapping, and single-molecule orientational imaging. Challenges and prospects in mSMLM are also discussed, which include the development of more vibrant and functional fluorescent probes, the optimization of optical implementation to judiciously utilize the photon budget, and the advancement of imaging analysis and machine learning techniques.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ba/6f/im3c00045.PMC10466381.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10148799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1