Chemical & Biomedical Imaging最新文献_第6页

Scanning Photoelectrochemical (Cell) Microscopy for In Situ Measurements of Photo(electro)catalysis 扫描光电化学（电池）显微镜用于光（电）催化的原位测量。

IF 6.7

Chemical & Biomedical Imaging Pub Date : 2026-07-27 Epub Date: 2026-02-03 DOI: 10.1021/cbmi.5c00272

Kyle Morgan, Namodhi Wijerathne, Md Yeasin Pabel, Bo-Lin Chen, Wei David Wei

{"title":"Scanning Photoelectrochemical (Cell) Microscopy for In Situ Measurements of Photo(electro)catalysis","authors":"Kyle Morgan, Namodhi Wijerathne, Md Yeasin Pabel, Bo-Lin Chen, Wei David Wei","doi":"10.1021/cbmi.5c00272","DOIUrl":"10.1021/cbmi.5c00272","url":null,"abstract":"Photo(electro)catalysis promises energy-efficient andselectivegeneration of chemical products. However, rational catalyst designis constrained by an incomplete understanding of the underlying mechanisms,necessitating in situ, surface-sensitive, and spatiallylocalized characterization techniques. Scanning photoelectrochemical(cell) microscopy (SPECM/SPECCM) utilizes ultramicroelectrodes nearthe catalyst surface to spatially map structure-dependent activity,charge-transfer dynamics, and active site generation. By directlycorrelating local structure with catalytic behavior, critical insightsinto the factors governing catalytic activity, stability, and selectivityare gained, guiding the rational design of high-performance photo(electro)catalysts.In this review, we first introduce scanning electrochemical techniquesfollowed by a detailed description of common methods of light integration,underscoring their individual strengths and limitations. We then highlightthe ways SPECM and SPECCM have been utilized to gain information onstructure-dependent activity in anisotropic materials, charge-transferin heterostructures, and photogenerated active site formation. Weconclude with an outlook on potential research directions to furtheradvance the mechanistic insights obtainable through scanning photoelectrochemicaltechniques.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 7","pages":"1278–1292"},"PeriodicalIF":6.7,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148622563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Step Expansion SMLM Enables Molecular-Resolution Imaging in Cells and Isolated Proteins 单步扩展SMLM实现细胞和分离蛋白的分子分辨率成像。

IF 6.7

Chemical & Biomedical Imaging Pub Date : 2026-07-27 Epub Date: 2026-05-13 DOI: 10.1021/cbmi.6c00054

Danush Taban, Marvin Jungblut, Made Budiarta, Dominic A. Helmerich, Cerridwen Kiesel, Sarah E. Plutkis, Luke D. Lavis, Donatus Krah, Ali H. Shaib, Sören Doose, Philip Kollmannsberger, Silvio O. Rizzoli, Gerti Beliu, Markus Sauer

引用次数: 0

In Situ Electrochemical Monitoring of Bacillus cereus Biofilm Formation 蜡样芽孢杆菌生物膜形成的原位电化学监测。

IF 6.7

Chemical & Biomedical Imaging Pub Date : 2026-07-27 Epub Date: 2026-02-16 DOI: 10.1021/cbmi.5c00271

Aleksandar Mijajlovic, Dalibor Stankovic, Milica Sentic, Vlad Costache, Shanshan Wang, Hadi Jbara, Julien Deschamps, Romain Briandet, Neso Sojic, Jasmina Vidic

{"title":"In Situ Electrochemical Monitoring of Bacillus cereus Biofilm Formation","authors":"Aleksandar Mijajlovic, Dalibor Stankovic, Milica Sentic, Vlad Costache, Shanshan Wang, Hadi Jbara, Julien Deschamps, Romain Briandet, Neso Sojic, Jasmina Vidic","doi":"10.1021/cbmi.5c00271","DOIUrl":"10.1021/cbmi.5c00271","url":null,"abstract":"Unwanted biofilms pose significantchallenges in the food industry,on medical devices, in water supply systems and marine environments,and even in space exploration. Label-free, advanced sensing approachessuch as electrical impedance spectroscopy (EIS) have been investigatedas tools for monitoring biofilm formation. The electrode materialand the surrounding medium, differing in carbon source, ionic strength,or nutrient availability, can strongly influence bacterial metabolicactivity, extracellular polymeric substance (EPS) production, andconsequently the resulting impedimetric parameters. Here, we report Bacillus cereus (B. cereus) biofilm formationon gold and indium tin oxide (ITO) surfaces in two bacterial mediathat promote biofilm formation. We characterized the impedance responsesof biofilms formed at different time points after inoculation anddemonstrated a direct correlation between variations in charge transferresistance and biofilm structure. Non-Faradaic EIS, confocal laserscanning microscopy, and scanning electron microscopy all indicatedthat B. cereus exhibited faster biofilm developmenton ITO than on gold, possibly due to differences in surface chargeor antibacterial effects associated with the nanostructured gold surface.We demonstrate a strong correlation between EIS measurements and microscopicimaging observations of early biofilm formation and the extent ofbiofilm development. This combination of methodologies provides areliable approach for detecting and characterizing biofilms, particularlygiven the high heterogeneity and dynamic behavior of microbial communities.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 7","pages":"1361–1369"},"PeriodicalIF":6.7,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148622530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic Drugs: Concept, Current Status, and Challenges in New-Age Diagnosis Platform Development 诊断药物：新时代诊断平台发展的概念、现状与挑战

IF 6.7

Chemical & Biomedical Imaging Pub Date : 2026-07-27 Epub Date: 2026-01-30 DOI: 10.1021/cbmi.5c00236

Suresh K. Kalangi, Rajesh S. Bhosale, Tejender S. Thakur

{"title":"Diagnostic Drugs: Concept, Current Status, and Challenges in New-Age Diagnosis Platform Development","authors":"Suresh K. Kalangi, Rajesh S. Bhosale, Tejender S. Thakur","doi":"10.1021/cbmi.5c00236","DOIUrl":"10.1021/cbmi.5c00236","url":null,"abstract":"Unmet medical needs form the basis for the discoveryof diagnostictools and drugs for diseases that elude existing treatment options.Herein, we provide a perspective on the current trends in diagnosisand treatment using conventional and state-of-the-art diagnostic drugs.Our report highlights the evolution of diagnosis and therapy, moreso in the form of after pulmonary combining the drug discovery processwith diagnostics known as “drug-diagnostic codevelopment”,which has opened the doors for precision medicine. Modern-age diagnosticdrugs, such as conjugating a drug to fluorophores via a covalent bond,have the potential to both detect and treat diseases. However, fluorescencequenching in solutions is a major bottleneck. This can be addressedusing quantum dots (QDs) conjugated to drugs such as cadmium telluride(CdTe) QDs conjugated to 5-fluorouracil (5-FU), naproxen, and celecoxib.However, these conjugates could get aggregated, and they may alsopose health risks due to the involvement of heavy metals. Supramolecularchemistry could play a pivotal role in addressing both the aggregationand safety issues without compromising its utility. The discoveryof supramolecular aggregation-induced emission (AIE) probes and theirapplications to drug conjugates have been demonstrated in a few studies.Taken together, this report summarizes the significance of advanceddiagnostic drugs possessing AIE-conjugated probes for use in diagnosisand therapy.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 7","pages":"1248–1257"},"PeriodicalIF":6.7,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148622522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research on the Application of Fluorescent Probes in Revealing the Mechanism of Cerebral Ischemia-Reperfusion Injury 荧光探针在揭示脑缺血再灌注损伤机制中的应用研究。

IF 6.7

Chemical & Biomedical Imaging Pub Date : 2026-07-27 Epub Date: 2026-02-10 DOI: 10.1021/cbmi.5c00254

Yan Shi, Jie Lv, Na Zhou, Fangjun Huo, Caixia Yin

{"title":"Research on the Application of Fluorescent Probes in Revealing the Mechanism of Cerebral Ischemia-Reperfusion Injury","authors":"Yan Shi, Jie Lv, Na Zhou, Fangjun Huo, Caixia Yin","doi":"10.1021/cbmi.5c00254","DOIUrl":"10.1021/cbmi.5c00254","url":null,"abstract":"Despite being a key treatment for ischemic stroke─abraininjury with high incidence, mortality, disability, and recurrencerates─reperfusion therapy can cause secondary cerebral ischemia-reperfusioninjury (CIRI). During the course of CIRI, a large amount of reactiveoxygen species (ROS) and reactive nitrogen species (RNS) burst out,causing cellular redox imbalance, followed by cellular oxidative stress,and ultimately leading to neuronal death. ROS and reactive sulfurspecies (RSS) are two important oxidizing and reducing substancesin the body, playing a significant role in regulating redox homeostasis.Therefore, real-time monitoring of their levels and dynamic changescan provide effective strategies for alleviating CIRI. However, organicsmall molecule probes have deficiencies in selectivity and blood–brainbarrier (BBB) permeability, making real-time monitoring of activespecies changes during CIRI in the brain more difficult. Therefore,this review summarizes the application and research progress of organicsmall molecule fluorescent probes in the field of CIRI in the pastfive years, and classifies them according to design strategies, whichis expected to provide guidance for the subsequent development oforganic small molecule fluorescent probes for CIRI and expand theirapplication in the biomedical field.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 7","pages":"1258–1277"},"PeriodicalIF":6.7,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148622471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Histological Processing on Endogenous Elemental Contents and Distributions in Breast Cancer Tissue Sections 组织学处理对乳腺癌组织切片内源性元素含量和分布的影响。

IF 6.7

Chemical & Biomedical Imaging Pub Date : 2026-07-27 Epub Date: 2026-02-04 DOI: 10.1021/cbmi.5c00217

Amy V. Lovejoy, Alexander P. Morrell, Diego Mesa, Alex Griffiths, Piotr R. Golda, Kay L. Dawson, Evie Wren, Katharina Kreissig, Charles Coombes, Mark Rehkämper, Rebekah E.T. Moore

{"title":"Impact of Histological Processing on Endogenous Elemental Contents and Distributions in Breast Cancer Tissue Sections","authors":"Amy V. Lovejoy, Alexander P. Morrell, Diego Mesa, Alex Griffiths, Piotr R. Golda, Kay L. Dawson, Evie Wren, Katharina Kreissig, Charles Coombes, Mark Rehkämper, Rebekah E.T. Moore","doi":"10.1021/cbmi.5c00217","DOIUrl":"10.1021/cbmi.5c00217","url":null,"abstract":"Trace metal homeostasis is criticalfor cellular functionand oftendisrupted in diseases such as cancer. Spatially resolved techniquesenable mapping of metal distributions within complex cellular environments,surpassing the limitations of bulk analyses. Formalin-fixed, paraffin-embedded(FFPE) tissues are widely used in research, yet the impact of FFPEprocessing on endogenous elemental contents and distributions remainsunclear, with conflicting findings across sample types. This studyevaluates the suitability of FFPE human breast carcinoma tissue sectionsfor multielemental bioimaging by direct comparison with paired freshfrozen (FF) tissues and assesses potential sources of contaminationor elemental losses during processing. Three FF human breast carcinomaspecimens were bisected and processed as either FF or FFPE. Serialsections underwent analyses by laser ablation inductively coupledplasma mass spectrometry (LA-ICP-MS), alongside hematoxylin and eosin(H&E)-staining, to compare elemental profiles in whole sectionsand morphologically distinct regions. In parallel, FFPE processingreagents encompassing tissue-marking dyes, formalin, paraffin wax,xylene, and ethanol were analyzed via solution ICP-MS to contextualizethe in situ findings. The multielemental imagingresults revealed that Zn, Mn, and Fe were largely preserved in FFPEsections, supporting the continued use of such tissues for in situ metal analyses. Addition of Cu was observed forall FFPE samples, most likely caused by exposure to blue tissue-markingdye. Formalin induced variable elemental leaching, most notably affectingMg. Paraffin wax, ethanol, and xylene contained minimal trace metallevels both before and after contact with tissues.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 7","pages":"1390–1404"},"PeriodicalIF":6.7,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148622503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Versatile Electrochemiluminescence Imaging Platform for Multiplexed Biosensing and Point-of-Care Application 多功能电化学发光成像平台，用于多路生物传感和护理点应用。

IF 6.7

Chemical & Biomedical Imaging Pub Date : 2026-07-27 Epub Date: 2026-02-06 DOI: 10.1021/cbmi.5c00274

Stephania Rodríguez Muiña, Gabriele Giagu, Giovanni Valenti, Francisco Javier del Campo

{"title":"Versatile Electrochemiluminescence Imaging Platform for Multiplexed Biosensing and Point-of-Care Application","authors":"Stephania Rodríguez Muiña, Gabriele Giagu, Giovanni Valenti, Francisco Javier del Campo","doi":"10.1021/cbmi.5c00274","DOIUrl":"10.1021/cbmi.5c00274","url":null,"abstract":"Electrochemiluminescence (ECL) offershigh analytical sensitivity,low optical background, and spatially confined light emission at electrodeinterfaces, enabling a large global market for bead-based ECL immunoassays.Existing ECL instrumentation remains bulky, costly, and largely confinedto centralized laboratories with limited access to true spatiallyresolved imaging. Here, we present a compact, modular ECL imagingplatform that enables low-light, spatially resolved, and temporallysynchronized electrochemical–optical measurements. The systemintegrates a Raspberry Pi 4 controller, a monochrome OV9281 global-shuttercamera, and an EmStat4s potentiostat, coordinated through open-sourcePython software providing sub-100 ms synchronization and full controlof camera parameters (resolution, exposure, gain, frame rate, andfocus). The complete system, of approximately 21 × 15 ×21 cm, has a component bill of materials of 2337 €, which couldbe reduced to a few hundred euros by using a custom potentiostat,making it substantially more affordable than commercial ECL instruments.Platform performance was validated using both homogeneous solution-phaseand heterogeneous bead-based [Ru(bpy)3]2+/tripropylamine(TPrA) assays on carbon screen-printed electrodes. Synchronized cyclicvoltammetry and chronoamperometry captured ECL transient behavior,including onset, peak emission, and decay dynamics. Quantitative bead-basedassays showed excellent linearity with low-picomolar range limitsof detection, while spatially resolved analysis enabled multiplexedmeasurements on a single electrode via a region-of-interest readout.These results demonstrate the importance of controlled and optimizedcamera parameters for quantitative ECL imaging and establish thiscompact, low-cost, and modular platform as a practical alternativefor spatially resolved ECL biosensing and multiplex point-of-careapplications.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 7","pages":"1491–1500"},"PeriodicalIF":6.7,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148622644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface Plasmon Resonance Imaging and Microscopy Modalities for Information-Rich, Label-Free Analysis of Biomolecular Interactions and Disease Biomarkers 表面等离子体共振成像和显微技术用于信息丰富、无标记的生物分子相互作用和疾病生物标志物分析。

IF 6.7

Chemical & Biomedical Imaging Pub Date : 2026-07-27 Epub Date: 2026-02-19 DOI: 10.1021/cbmi.5c00290

Victor A. Hanson, Westley Van Zant, Cole P. Ebel, Alexander S. Malinick, Daniel D. Stuart, Luke N. Stemple, Quan Cheng

{"title":"Surface Plasmon Resonance Imaging and Microscopy Modalities for Information-Rich, Label-Free Analysis of Biomolecular Interactions and Disease Biomarkers","authors":"Victor A. Hanson, Westley Van Zant, Cole P. Ebel, Alexander S. Malinick, Daniel D. Stuart, Luke N. Stemple, Quan Cheng","doi":"10.1021/cbmi.5c00290","DOIUrl":"10.1021/cbmi.5c00290","url":null,"abstract":"Label-free imaging techniques arepowerful tools forcharacterizingbiomolecular interactions, offering important advantages over traditionalfluorescence-based imaging methods. Among these approaches, surfaceplasmon resonance imaging (SPRi) has emerged as a particularly versatileand enabling platform owing to its simple experimental configuration,rapid data acquisition, and high imaging sensitivity. A related variant,surface plasmon resonance microscopy (SPRM), further extends the capabilitiesof SPR-based sensing by providing enhanced spatial resolution andexpanded sensing depth, allowing interrogation of interactions atthe single-particle and single-event level. The combined versatilityof SPRi and SPRM has supported a wide range of applications, includingmolecular recognition, quantitative interaction analysis, extracellularvesicle detection, and nanobubble characterization. More recently,the integration of machine-learning approaches into both instrumentaldevelopment and postacquisition data analysis has significantly enhancedthe ability of SPR-based imaging techniques to address complex sampleenvironments, enabling multiplexed, high-throughput, and information-richmeasurements. This review provides a comprehensive overview of recentadvances in SPRi and SPRM, with a particular emphasis on innovativemethodological developments and emerging applications within the broaderSPR research landscape. Key topics include advances in optical configuration,machine-learning-assisted analysis, nanoparticle and nanoscale objectcharacterization, and the development of sensing platforms for biomarkerassessment.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 7","pages":"1315–1337"},"PeriodicalIF":6.7,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148622608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[18F]AlF-RESCA-R8B4 and [68Ga]Ga-NOTA-R8B4: A Pair of Novel CD70-Targeted ImmunoPET/CT Imaging Agents for Clinical Use [18F]AlF-RESCA-R8B4和[68Ga]Ga-NOTA-R8B4：一对新型cd70靶向免疫pet /CT显像剂

IF 6.7

Chemical & Biomedical Imaging Pub Date : 2026-07-27 Epub Date: 2026-02-20 DOI: 10.1021/cbmi.6c00002

You Zhang, Qianyun Wu, Shuxian An, Dongsheng Xu, Wenzhi Jia, Zhoumi Hu, Cheng Wang, Gang Huang, Jin Zhang, Jianjun Liu, Weijun Wei

{"title":"[18F]AlF-RESCA-R8B4 and [68Ga]Ga-NOTA-R8B4: A Pair of Novel CD70-Targeted ImmunoPET/CT Imaging Agents for Clinical Use","authors":"You Zhang, Qianyun Wu, Shuxian An, Dongsheng Xu, Wenzhi Jia, Zhoumi Hu, Cheng Wang, Gang Huang, Jin Zhang, Jianjun Liu, Weijun Wei","doi":"10.1021/cbmi.6c00002","DOIUrl":"10.1021/cbmi.6c00002","url":null,"abstract":"Precise diagnosis of primary and metastatic clear cellrenal cellcarcinoma (ccRCC) is an unmet clinical challenge. CD70 is a biomarkerfor ccRCC, with high, consistent expression in both primary and metastaticdiseases. Devising immuno-positron emission tomography/computed tomography(immunoPET/CT) imaging tracers targeting CD70 will enable noninvasive,accurate diagnosis of ccRCC. By immunizing alpacas with the recombinanthuman CD70 protein, we obtained a single-domain antibody (sdAb), R8B4,with an affinity of 9.583 nM. First, by harnessing 18Flabeling, we developed a CD70 immunoPET/CT tracer, [18F]AlF-RESCA-R8B4,and verified its diagnostic accuracy in preclinical ccRCC models.Subsequently, the more readily available [68Ga]Ga-NOTA-R8B4was constructed using 68Ga labeling, and its targetingperformance was also validated. Compared with [18F]AlF-RESCA-R8B4,[68Ga]Ga-NOTA-R8B4 showed lower renal accumulation. Followingthis, we conducted a preliminary investigator-initiated clinical trialthat demonstrated that [18F]AlF-RESCA-R8B4 clearly visualizedCD70-positive metastatic lesions in ccRCC patients. With improvedpharmacokinetics and reduced kidney accumulation, [68Ga]Ga-NOTA-R8B4immunoPET/CT shows preliminary, yet promising, value for accuratelydetecting primary lesions in ccRCC patients. Future studies are warrantedto explore the diagnostic and predictive value of the CD70-targetedimmunoPET/CT imaging approaches in ccRCC patients.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 7","pages":"1521–1529"},"PeriodicalIF":6.7,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148622470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiamyloid Antibody Effects on Aβ-42 Protein Aggregates Profiled Using Nanospectroscopy 抗淀粉样蛋白抗体对Aβ-42蛋白聚集体的影响

IF 6.7

Chemical & Biomedical Imaging Pub Date : 2026-07-27 Epub Date: 2026-02-19 DOI: 10.1021/cbmi.5c00188

Nico Kummer, Martina Cihova, Peter Niraj Nirmalraj

{"title":"Antiamyloid Antibody Effects on Aβ-42 Protein Aggregates Profiled Using Nanospectroscopy","authors":"Nico Kummer, Martina Cihova, Peter Niraj Nirmalraj","doi":"10.1021/cbmi.5c00188","DOIUrl":"10.1021/cbmi.5c00188","url":null,"abstract":"Anti-amyloid-β (Aβ) monoclonal antibodiesare designedto selectively target protein biomarkers and promote amyloid clearancein Alzheimer’s disease. Yet the effects of antibodies on individualAβ protein aggregates remain to be fully clarified. The workpresented here employs nanospectroscopy (atomic force microscopy combinedwith infrared spectroscopy) to resolve morphological and secondarystructural changes in Aβ-42 protein aggregates upon treatmentwith aducanumab and lecanemab antibodies (biosimilars). The treatmentof Aβ-42 peptides with aducanumab resulted in reduction of oligomerprevalence and formation of larger-diameter fibril bundles confirmedfrom the AFM height maps and infrared spectral readouts recorded atnanometer-scale spatial resolution. Conversely, lecanemab treatmentof Aβ-42 peptides did not suppress oligomer generation but wasobserved to preferentially bind along the full length of the nodular-shapedprotofibril surface. Importantly, neither aducanumab nor lecanemabwas observed to induce any surface adsorption-driven disassembly ofAβ-42 protofibrils or elongated mature fibrils. Thus, nanospectroscopyenables direct characterization of antibody–amyloid interfacialinteractions and provides insights into the distinct modes of actionof emerging anti-Aβ therapeutics through label-free chemicalimaging.","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 7","pages":"1379–1389"},"PeriodicalIF":6.7,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148622500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0